Improving the method for determining the physiological and sanitary potential of Gherkin seeds

Gherkin seeds usually show irregular physiological quality. Seed production requires fast and reliable tests to evaluate seed quality. Germination test is considered a recognized analysis method; however, seed technology has pursuit the improvement of vigor tests aiming the evaluation of seed’s physiological potential. Thus, the objective of this work was to evaluate procedures to perform the test of accelerated aging and determine the physiological and sanitary potential of gherkin seeds. Four seed lots of cultivar Liso Calcuta were used in the study. To evaluate the initial physiological quality the water content was determined and germination and emergence tests, as well as indices of germination speed and emergence speed were used. The accelerated aging test was performed as traditionally and with saturated saline solution, with 48, 72 and 96 hours, at temperatures of 41oC and 45oC. After aging, the water content was determined, and seeds’ germination and sanity tests were performed. The experiment was set under a completely random design in factorial 4x3x2 (lots x aging periods x temperatures). The standard accelerated aging test and the test with saturated saline solution at 41oC for 96 hours were efficient to evaluate the vigor of gherkin seeds. Saturated saline solution provides uniform water absorption and deterioration in gherkin seeds, allowing to discriminate seed lots in different vigor levels. The salinity test after accelerated aging with saline solution reduces the incidence of some fungi.

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Accelerated Aging Test to Determine the Vigor of Mungbean Seeds

Journal of Agricultural Science ◽

10.5539/jas.v11n7p42 ◽

2019 ◽

Vol 11 (7) ◽

pp. 42

Author(s):

Luciana Maria da Silva ◽

Anderson Dias Vaz de Souza ◽

Rogério Lámim Silva Junior ◽

Natália Arruda ◽

Nei Peixoto ◽

...

Keyword(s):

Seed Quality ◽

Germination Rate ◽

Accelerated Aging ◽

Dry Weight ◽

Precise Determination ◽

Germination Test ◽

Accelerated Aging Test ◽

Seedling Length ◽

Aging Test ◽

Randomized Experimental Design

The use of good quality seeds is fundamental for proper establishment of a crop. In this way, for more precise determination of seed quality, vigor tests are performed in addition to the germination test. These tests enable the selection of the best lots for commercialization and planting. This study evaluates the effects of temperature and exposure times of the accelerated aging test for vigor classification of mungbean seed lots. Seeds of the mungbean cultivar Esmeralda were used, being obtained from four cultivated plots at the State University of Goiás (UEG), Ipameri Câmpus, in the 2013, 2015, 2016, and 2017 harvests. The lots were initially characterized using germination and vigor tests (first germination count, germination rate index, seedling length, and seedling fresh and dry weight). The accelerated aging test was conducted with a completely randomized experimental design, in a 2 × 4 factorial arrangement consisting of two temperatures (40 and 42 °C) and four times (24, 48, 72, and 96 hours), with four replicates of 50 seeds per lot. The lots showed significant differences in the germination test, first germination count, germination rate index, and seedling length. The accelerated aging test was efficient in classifying lots. Lot 3 obtained the best results, while lot 1 obtained the lowest ones. The combination of 42 °C temperature and 72 h of seed exposure to the accelerated aging test is the best to classify mungbean seed lots.

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Tests for evaluating the physiological quality of pitaya seeds

Semina Ciências Agrárias ◽

10.5433/1679-0359.2015v36n6sup2p4047 ◽

2015 ◽

Vol 36 (6Supl2) ◽

pp. 4047

Author(s):

Thiago Alberto Ortiz ◽

Aline Moritz ◽

Lúcia Sadayo Assari Takahashi ◽

Mariana Ragassi Urbano

Keyword(s):

Electrical Conductivity ◽

Pearson Correlation ◽

Accelerated Aging ◽

Seed Vigor ◽

Water Volume ◽

Germination Test ◽

Accelerated Aging Test ◽

Aging Test ◽

Physiological Quality

Germination test is used to assess the physiological quality of seeds; however, since it is carried out under ideal conditions, this test has not been shown sufficient for this purpose. Instead, it is possible to use vigor tests, although the lack of standardized methodologies has reduced their applicability and reproducibility. Thus, this study aimed to develop methodologies for conducting tests of germination, accelerated aging, and electrical conductivity for the evaluation of the physiological quality of pitaya seeds. For this purpose, seeds from ripe Hylocereus undatus fruits were used. A completely randomized experimental design was used with four replications. The physiological quality of the seeds was assessed using germination, accelerated aging, and electrical conductivity tests, and the speed of germination index (SGI) and mean germination time (MGT) were determined for both the germination test and accelerated aging test. For the statistical analysis, we performed regression model adjustments and calculated the Pearson correlation coefficient (p < 0.05). The germination test for H. undatus seeds can be performed at 25 °C, with the aim of reaching the highest SGI and lowest MGT values. The accelerated aging test can be conducted at 43 °C for 48 h, because combining these factors favors the expression of seed vigor, allowing seeds to achieve the maximum SGI and minimum MGT, while reducing the time of the assay. The electrical conductivity test can be performed using 25 seeds at a temperature of 30 °C and a water volume of 10 mL, since under these conditions there is less interference from external factors on the leachate content of the solution.

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QUALIDADE FISIOLÓGICA DE SEMENTES DE SOJA SUBMETIDAS À DESSECAÇÃO EM PRÉ-COLHEITA

Colloquium Agrariae ◽

10.5747/ca.2018.v14.n4.a254 ◽

2018 ◽

Vol 14 (4) ◽

pp. 112-120

Author(s):

Renan Canzi Comin ◽

Geri Eduardo Meneghello ◽

Jader Job Franco ◽

Alexandre Moscarelli Levien ◽

Kassiana Kehl ◽

...

Keyword(s):

Seed Quality ◽

Accelerated Aging ◽

Glufosinate Ammonium ◽

Accelerated Aging Test ◽

Aging Test ◽

Physiological Quality ◽

Important Idea ◽

The City ◽

Technical Recommendations

The use of high quality soybean seeds (Glycine maxL.) is one of the most important aspects to obtain satisfactory productivity indexes. The desiccation practice is an important idea of the producers to anticipate theharvest and to minimize the rapiddeterioration of seed quality in the field. In this context, the objective of this work was to evaluate the effect of the use of desiccants applied in R7stageon the physiological quality of soybean seeds.The experiment was conducted in experimental area, located in the city of Passo Fundo, Rio Grande do Sul. Four soybean cultivars (FPS Jupiter RR, FPS Paranapanema RR, BRS Tordilha RR and FPS Urano RR) were planted under experimental design, managed according to technical recommendations for the crop.Upon reaching the R7 stage, different desiccation treatments (without desiccation, paraquat, diquat and glufosinate ammonium) were applied to the subplot.The seeds were harvested with a plot harvester and the weight of one thousand seeds, germination test and accelerated aging test were evaluated, being the last two evaluated at 30 and 180 days after harvest. The pre-harvest desiccation at the R7 stage of soybean cultivation did not negatively affect the physiological quality of the seeds and, generally way, for the cultivars FPS Jupiter RR and FPS Urano RR, it was possible to obtain more vigorous seeds at 30 DAC. Under the conditions under which the experiment was conducted, the diquat, paraquat and glufosinate ammonium herbicides can be used for pre-harvest desiccation in the seed production fields.

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Saturated salt accelerated aging and computerized analysis of seedling images to evaluate soybean seed performance

Journal of Seed Science ◽

10.1590/2317-1545v32n2935 ◽

2014 ◽

Vol 36 (2) ◽

pp. 213-221 ◽

Cited By ~ 5

Author(s):

Juliana Tieme Yagushi ◽

Denis Santiago Costa ◽

José de Barros França-Neto

Keyword(s):

Seed Quality ◽

Imaging System ◽

Seedling Emergence ◽

Soybean Seed ◽

Accelerated Aging ◽

Seed Vigor ◽

Accelerated Aging Test ◽

Aging Test ◽

Physiological Quality ◽

Tetrazolium Test

Seed deterioration causes losses to seed companies during the storage. For researches, the challenge has been to develop techniques which improve the seed longevity as well as laboratory analysis methods required for evaluating the evolution of deterioration in storage. This study had the objective of determining the effectiveness of the accelerated aging test with a saturated NaCl solution and Seed Vigor Imaging System (SVIS) analysis in checking soybean seed physiological in comparison with the recommended vigor tests. Soybean seeds from the cultivars BRS 184 and M-Soy 7908 RR were used, each of them were represented by six lots and stored for six months under two environment conditions. Seed physiological quality was checked every two months in storage through germination evaluations, accelerated aging (different methods), tetrazolium test (vigor and viability) seedling emergence, and SVIS analysis. The tetrazolium test is the most advisable method for evaluating soybean seed quality during storage. The results of the saturated salt accelerated aging test with NaCl at 41 ºC for 72 or 96 hours and the SVIS evaluation are also both efficient for estimating the physiological potential of seeds.

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Physiological quality of soybean seeds of cultivars submitted to harvesting delay and its association with seedling emergence in the field

Journal of Seed Science ◽

10.1590/s2317-15372013000200002 ◽

2013 ◽

Vol 35 (2) ◽

pp. 147-152 ◽

Cited By ~ 12

Author(s):

Fábio Oliveira Diniz ◽

Múcio Silva Reis ◽

Luiz Antônio dos Santos Dias ◽

Eduardo Fontes Araújo ◽

Tuneo Sediyama ◽

...

Keyword(s):

Seed Quality ◽

Seedling Emergence ◽

Accelerated Aging ◽

Soybean Seeds ◽

Test Results ◽

Accelerated Aging Test ◽

Aging Test ◽

Physiological Quality ◽

The Relationship

With the aim of studying the relationship between the physiological quality of soybean seeds submitted to three harvest times and seedling emergence in the field, seeds of cultivars UFV-16, Splendor, Vencedora, Confiança, UFV-18, UFV-TN 105, Garantia and Celeste were harvested at stages R8, R8+15 and R8+30 days. The following tests were performed: accelerated aging, electrical conductivity, emergence in sand, emergence speed index and seedling emergence in the field. Germination and vigor were reduced in seeds harvested at 30 days after stage R8 in all the tests, except for the accelerated aging test, which showed a decline in vigor seeds harvested with a delay of 15 days. Seeds of cultivars UFV-16, Confiança and Garantia showed lower physiological quality in most tests, while the seeds of cultivar Celeste had higher physiological quality than the others. There was similarity between the test results of seed quality and seedling emergence in the field.

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Parameters of the Accelerated Aging Test for the Determination of Tobacco (Nicotiana tabacum L.) Seed Vigor

Journal of Agricultural Studies ◽

10.5296/jas.v8i1.15828 ◽

2020 ◽

Vol 8 (1) ◽

pp. 247

Author(s):

Luis Henrique Konzen ◽

Daniele Brandstetter Rodrigues ◽

Alcimar Spindola Mazon ◽

William Aldrei Guareschi ◽

Jerffeson Araujo Cavalcante ◽

...

Keyword(s):

Saline Solution ◽

Seedling Emergence ◽

Accelerated Aging ◽

Exposure Period ◽

Seed Vigor ◽

Germination Test ◽

Accelerated Aging Test ◽

Tobacco Seeds ◽

Aging Test

Vigor tests are very important to obtain additional information to the standard germination test and can assist in decision-making. The aim of this work was to evaluate accelerated aging test methodologies for the determination of vigor in tobacco seeds. For the study, ten seed lots of tobacco were used. The initial quality of the tobacco seeds was determined through the germination test, first count of germination, germination speed index, seedling emergence at 7, 14 and 21 days after sowing, emergence speed index and the accelerated aging with water performed as proposed by the Association of Official Seed Analysts. After the determination of seed initial quality, the accelerated aging test was studied in the following methods: accelerated aging with water, saturated saline solution (40g NaCl 100mL-1 water) and unsaturated saline solution (11g NaCl 100mL-1 water), submitted to the temperatures of 45 and 41°C, for the exposure periods of 24, 48 and 72 hours. According to the results obtained, the accelerated aging test with water conducted under a temperature of 45°C combined with a 24-hour exposure period is shown as adequate for evaluating the vigor of tobacco seeds.

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Tests for evaluating the physiological quality of pitaya seeds

Semina Ciências Agrárias ◽

10.5433/1679-0359.2015v36n6supl2p4047 ◽

2015 ◽

Vol 36 (6Supl2) ◽

pp. 4047

Author(s):

Thiago Alberto Ortiz ◽

Aline Moritz ◽

Lúcia Sadayo Assari Takahashi ◽

Mariana Ragassi Urbano

Keyword(s):

Electrical Conductivity ◽

Pearson Correlation ◽

Accelerated Aging ◽

Seed Vigor ◽

Water Volume ◽

Germination Test ◽

Accelerated Aging Test ◽

Aging Test ◽

Physiological Quality

Germination test is used to assess the physiological quality of seeds; however, since it is carried out under ideal conditions, this test has not been shown sufficient for this purpose. Instead, it is possible to use vigor tests, although the lack of standardized methodologies has reduced their applicability and reproducibility. Thus, this study aimed to develop methodologies for conducting tests of germination, accelerated aging, and electrical conductivity for the evaluation of the physiological quality of pitaya seeds. For this purpose, seeds from ripe Hylocereus undatus fruits were used. A completely randomized experimental design was used with four replications. The physiological quality of the seeds was assessed using germination, accelerated aging, and electrical conductivity tests, and the speed of germination index (SGI) and mean germination time (MGT) were determined for both the germination test and accelerated aging test. For the statistical analysis, we performed regression model adjustments and calculated the Pearson correlation coefficient (p < 0.05). The germination test for H. undatus seeds can be performed at 25 °C, with the aim of reaching the highest SGI and lowest MGT values. The accelerated aging test can be conducted at 43 °C for 48 h, because combining these factors favors the expression of seed vigor, allowing seeds to achieve the maximum SGI and minimum MGT, while reducing the time of the assay. The electrical conductivity test can be performed using 25 seeds at a temperature of 30 °C and a water volume of 10 mL, since under these conditions there is less interference from external factors on the leachate content of the solution.

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Accelerated Aging Test in Thyme Seeds (Thymus vulgaris L.)

Journal of Agricultural Studies ◽

10.5296/jas.v9i1.17761 ◽

2020 ◽

Vol 9 (1) ◽

pp. 32

Author(s):

Flávia Mendes dos Santos Lourenço ◽

Marco Eustáquio de Sá ◽

Oscar Mitsuo Yamashita ◽

Vinícius Martins Silva ◽

Juliana Trindade Martins ◽

...

Keyword(s):

Exposure Time ◽

Saline Solution ◽

Seed Quality ◽

Accelerated Aging ◽

Cold Test ◽

Dry Mass ◽

Thymus Vulgaris ◽

Aging Period ◽

Accelerated Aging Test ◽

Aging Test

Evaluating seed quality with precision and accuracy is one of the priorities for obtaining high quality material. This study aimed to evaluate whether the variations in the performance of the accelerated aging test, in terms of exposure time and temperature, in the traditional form or in saline solution, would result in an efficient combination to evaluate the physiological quality of thyme seeds (Thymus vulgaris L.). The seeds were submitted to the tests of total germination, germination speed index, first germination count, seedlings emergence, seedling length, seedling dry mass, cold test, electrical conductivity, variations in the conduction of the accelerated aging test and the water content of the seeds was performed after each accelerated aging period. The treatments were the combination of temperatures (36, 38, 40 and 42 ºC), exposure time (24, 36, 48 and 72 hours) and two procedures (traditional and saline), with four replications for each batch. Traditional accelerated aging test at 42 ºC and 24 hours of exposure time, and saline solution accelerated aging test at 38 ºC and 24 hours of exposure time showed sensibility to evaluate physiological potential for thyme seed. For both saline solution and traditional accelerated aging test, the exposition time of 24 hours was efficient to evaluate physiological potential of thyme seeds.

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ACCELERATED AGING AND ELECTRICAL CONDUCTIVITY TESTS IN CRAMBE SEEDS

Ciência e Agrotecnologia ◽

10.1590/s1413-70542015000100001 ◽

2015 ◽

Vol 39 (1) ◽

pp. 7-14 ◽

Cited By ~ 4

Author(s):

Juliana Joice Pereira Lima ◽

Marcella Nunes de Freitas ◽

Renato Mendes Guimarães ◽

Antônio Rodrigues Vieira ◽

Maria Alice Bento Ávila

Keyword(s):

Electrical Conductivity ◽

Chloride Solution ◽

Accelerated Aging ◽

Germination Test ◽

Crambe Seed ◽

Accelerated Aging Test ◽

Aging Test ◽

Physiological Quality

The aim of this study was to adapt the methodology of the accelerated aging and electrical conductivity tests for determination of physiological potential in crambe seeds. Six seed lots of crambe (cv. FMS Brilhante) were subjected to determination of moisture content, germination test, first count germination, emergence, and emergence speed index. For the accelerated aging test, the traditional methodology was used with water, and with a saturated potassium chloride and sodium chloride solution in three periods of exposure (24, 48, and 72 hours) at 41°C; the electrical conductivity test was performed with four pre-soaking treatments (0, 2, 4, and 8 hours) and four soaking periods (4, 8, 16, and 24 hours) at 25°C. The accelerated aging test with water for 72 hours and the electrical conductivity test with 2 hours of pre-soaking and assessment after 16 hours were effective for classification of the crambe seed lots in regard to physiological quality.

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STRESS CONDITIONS AND ITS EFFECTS ON THE PHYSIOLOGICAL POTENTIAL OF PAPAVER SP. SEEDS

Revista Científica Rural ◽

10.30945/rcr-v21i3.3087 ◽

2019 ◽

Vol 21 (3) ◽

pp. 166-182

Author(s):

Andréa Bicca Noguez Martins ◽

Ivan Ricardo Carvalho ◽

Giordano Gelain Conte ◽

Anna dos Santos Suñe ◽

Bruna Barreto Dos Reis ◽

...

Keyword(s):

Saline Solution ◽

Seedling Emergence ◽

Accelerated Aging ◽

Accelerated Aging Test ◽

Aging Test ◽

Wide Range ◽

Poppy Seeds ◽

Physiological Quality ◽

Rate Of Emergence

The physiological quality of seeds is commonly characterized by the germination and vigor, which can be defined as the sum of attributes that confers the potential for seeds to germinate, rapidly emerge and result in normal seedlings under a wide range of environmental conditions. The purpose of the study was to test alternative methodologies for the accelerated aging test to evaluate the physiological potential of poppy seeds. Samples obtained from four batches were subjected to germination test at a temperature of 20 °C, traditional accelerated aging and accelerated aging diluted with saline solution (11 g of NaCl in 100 mL-1 of water) using three temperatures, 41 °C, 42 ºC and 43 ºC , for periods of 12 and 24 hours. The percentage of germination, the rate of emergence velocity, performed together with the emergence speed and the percentage of seedling emergence test were evaluated. It is concluded that the methodology of traditional accelerated aging with temperature of 43 °C and period of 12h shows to be promising for the verification of the physiological quality of poppy seeds.

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