scholarly journals Molecular typing of HLA class II antigens in a São Paulo population

1998 ◽  
Vol 21 (3) ◽  
pp. 301-305 ◽  
Author(s):  
A.C. Goldberg ◽  
J.M. Chiarella ◽  
M.L.C. Marin ◽  
C. Rosales ◽  
D. Banic ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Normal Population ◽  
Sao Paulo ◽  
Normal Sample ◽  
São Paulo ◽  
Chain Reaction ◽  
Essential Information ◽  
Polymerase Chain ◽  
Hla Dqa1 ◽  
The City

In the present paper we show data obtained from a normal population with a racially mixed profile typical of the city of São Paulo, State of São Paulo. Data were generated with polymerase chain reaction using sequence specific primers (PCR-SSP) for HLA-DRB and polymerase chain reaction followed by hybridization with sequence specific oligonucleotide probes (PCR-SSO) for HLA-DQA1 and HLA-DQB1 loci. HLA-DRB, DQA1, DQB1 and haplotype frequencies as well as common linkage disequilibria were found. This population was also shown to be in genetic equilibrium according to the Hardy-Weinberg law. HLA-DR typing of a normal sample from the city of Porto Velho, State of Rondonia, highlighted the importance of different sets of HLA profiles found in other regions of the country. This database provides essential information for screening studies of disease associations, forensic analyses and transplants.

scholarly journals Comparative performances of seven quantitative Reverse-Transcription Polymerase Chain Reaction assays (RT-qPCR) for detecting SARS-CoV-2 infection in samples from individuals suspected of COVID-19 in São Paulo, Brazil

2021 ◽  
Vol 1 (1-2) ◽  
pp. 100012
Author(s):  
Lucila Okuyama Fukasawa ◽  
Cláudio Tavares Sacchi ◽  
Maria Gisele Gonçalves ◽  
Ana Paula Silva Lemos ◽  
Samanta Cristine Grassi Almeida ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Reverse Transcription ◽  
Sao Paulo ◽  
São Paulo ◽  
Chain Reaction ◽  
Polymerase Chain

Prevalence ofMycoplasma genitaliumamong HIV-infected men in São Paulo city detected by realtime polymerase chain reaction

2010 ◽  
Vol 21 (1) ◽  
pp. 23-25 ◽  
Author(s):  
F A M da Costa ◽  
R C da Silva ◽  
L B Arruda ◽  
P Montanheiro ◽  
A J da Silva Duarte ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Sao Paulo ◽  
São Paulo ◽  
Chain Reaction ◽  
Polymerase Chain

scholarly journals Detection of Brazilian spotted fever infection by polymerase chain reaction in a patient from the state of São Paulo

2005 ◽  
Vol 100 (3) ◽  
pp. 277-279 ◽  
Author(s):  
Elvira Maria Mendes Nascimento ◽  
Flávia de Sousa Gehrke ◽  
Rosa Amélia Maldonado ◽  
Silvia Colombo ◽  
Luiz Jacintho da Silva ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Spotted Fever ◽  
Sao Paulo ◽  
The State ◽  
São Paulo ◽  
Chain Reaction ◽  
Brazilian Spotted Fever ◽  
Polymerase Chain

scholarly journals Detection of coronavirus-2 by real-time reverse transcription polymerase chain reaction in conjunctival swabs from patients with severe form of Coronavirus disease 2019 in São Paulo, Brazil

Clinics ◽  
2021 ◽  
Vol 76 ◽  
Author(s):  
Mariana Akemi Matsura Misawa ◽  
Tatiana Tanaka ◽  
Tomás Minelli ◽  
Pedro Gomes Oliveira Braga ◽  
Juliana Mika Kato ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Real Time ◽  
Reverse Transcription ◽  
Sao Paulo ◽  
Severe Form ◽  
São Paulo ◽  
Chain Reaction ◽  
Polymerase Chain

Dissemination of IMP-1 Metallo-β-Lactamase–Producing Acinetobacter Species in a Brazilian Teaching Hospital

2006 ◽  
Vol 27 (7) ◽  
pp. 742-747 ◽  
Author(s):  
Maria C. B. Tognim ◽  
Ana C. Gales ◽  
Andréia P. Penteado ◽  
Suzane Silbert ◽  
Hélio S. Sader
Keyword(s):  
Polymerase Chain Reaction ◽  
Teaching Hospital ◽  
Sao Paulo ◽  
São Paulo ◽  
Chain Reaction ◽  
Acinetobacter Species ◽  
Positive Polymerase Chain Reaction ◽  
Carbapenem Resistant ◽  
Resistant Strains ◽  
Polymerase Chain

Objective.To evaluate the emergence and dissemination of metallo-β-lactamase (MBL)–producing Acinetobacter species.Design.All carbapenem-resistant Acinetobacter strains (1 strain per patient) collected during the period 1993–2001 were evaluated.Setting.A Brazilian tertiary care teaching hospital (Hospital São Paulo, São Paulo).Methods.Seventy-three strains of carbapenem-resistant Acinetobacter species were recovered from the organism bank of the hospital. All isolates were tested for antimicrobial susceptibility by broth microdilution methods, and the production of MBL was initially assessed by phenotypic tests (MBL Etest strip and a disk approximation test). The MBL enzymes were identified by polymerase chain reaction using primers for blaIMP’, blaVIM’, and blaSPM’ followed by gene sequencing. Genetic similarity among the carbapenem-resistant strains was evaluated by automated ribotyping.Results.Only colistin and ampicillin-sulbactam showed reasonable in vitro activity against carbapenem-resistant isolates (97% and 74% of isolates susceptible, respectively). More than half of the isolates (55%) had a positive MBL phenotypic test result and a positive polymerase chain reaction result for blaIMP_1 The proportion of IMP-1–producing Acinetobacter isolates among carbapenem-resistant strains increased from 0% in the 1993-1997 period to 29% in 1998 and 100% in the 1999-2001 period. No carbapenem-resistant Acinetobacter isolates that harbored blaVIM or blaSPM were detected. Molecular typing results revealed 20 ribogroups among carbapenem-resistant isolates. During the study period of 1994-2001, we identified 2 major ribogroups, 52-1 (MBL-negative and MBL-positive strains) and 60-7 (MBL-positive strains), that had a coefficient of similarity of 0.85 or higher.Conclusions.Our results indicate that IMP-1–producing strains of Acinetobacter emerged in our institution in 1998. Since then, production of this MBL was detected not only in the major ribogroups of carbapenem-resistant Acinetobacter species but also among isolates that belonged to 17 distinct ribogroups, indicating that this important mechanism of antimicrobial resistance was disseminated among distinct clones.

scholarly journals Molecular diagnosis of the curly lettuce parasitic contamination from hydroponic cultivation from supermarkets

2020 ◽  
Vol 29 (4) ◽  
Author(s):  
Fernanda Pinto-Ferreira ◽  
Jonatan Batista Reis ◽  
Aline Ticiani Pereira Paschoal ◽  
Letícia Santos Balbino ◽  
Amanda Bertão-Santos ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Toxoplasma Gondii ◽  
Dna Extraction ◽  
Chain Reaction ◽  
Cryptosporidium Spp ◽  
Hydroponic Cultivation ◽  
Polymerase Chain ◽  
Fluctuation Method ◽  
The City ◽  
Commercial Kit

Abstract The consumption of vegetables has increased in recent years due to the search for a healthier diet that is rich in fiber and has fewer calories. To assess the parasitic contamination of lettuce sold in markets, a survey of parasites was carried out from a supermarket chain in the city of Londrina, Paraná. A total of thirty samples of lettuce were purchased in the ten markets visited, three in each, of which ten were conventionally cultivated, ten were hydroponically cultivated, and ten were organically cultivated. All samples were analyzed using the sedimentation methods of Hoffman, Pons and Janer and the fluctuation method of Faust and colleagues and Willis with adaptations. In addition, the samples were subjected to DNA extraction by a commercial kit and polymerase chain reaction to detect Toxoplasma gondii, Cryptosporidium spp. and Giardia spp., which are protozoa that cause food and waterborne parasitic outbreaks. All samples were negative for sedimentation and flotation techniques. One of the hydroponically cultivated samples was positive for T. gondii. The results demonstrate the risk of curly lettuce contamination from hydroponic cultivation and the need for proper cleaning of these foods before consumption.

scholarly journals Circulation of Dengue Virus Serotypes in the City of Makkah, Saudi Arabia, as Determined by Reverse Transcription Polymerase Chain Reaction

2017 ◽  
Vol 2017 ◽  
pp. 1-5 ◽  
Author(s):  
Sameer R. Organji ◽  
Hussein H. Abulreesh ◽  
Gamal E. H. Osman
Keyword(s):  
Polymerase Chain Reaction ◽  
Saudi Arabia ◽  
Dengue Virus ◽  
Dengue Fever ◽  
Reverse Transcription ◽  
Chain Reaction ◽  
Denv Serotypes ◽  
Polymerase Chain ◽  
Low Prevalence ◽  
The City

The present study was aimed to investigate the circulation of four dengue virus (DENV) serotypes in Makkah, Western Saudi Arabia. Blood samples were collected from 25 dengue fever-suspected patients and were subjected to molecular typing for DENV-1, DENV-2, DENV-3, and DENV-4 serotypes of dengue virus, by reverse transcription polymerase chain reaction (RT-PCR), using six sets of primers. Of the 25 samples, only six samples (24%) were found to be positive for dengue virus infection. The prevalence of DENV-1 was higher (50% of DENV-positive samples), as compared to DENV-2 (33.3%) and DENV-3 (16.6%) serotypes. The fourth serotype, DENV-4, was not detected in any of the DENV-positive samples. Although Makkah is considered endemic to dengue fever, we observed low prevalence of dengue virus in the city, which may be attributed to various factors. Nonetheless, the results presented herein confirm the circulation of DENV serotypes in the Western region of Saudi Arabia. To the best of our knowledge, the current study so far is the first report demonstrating the prevalence of the DENV-1 serotype in the city Makkah, Saudi Arabia.

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