scholarly journals Genetic variability among and within races of Heterodera glycines Ichinohe assessed by RAPD markers

2000 ◽  
Vol 23 (2) ◽  
pp. 223-229 ◽  
Author(s):  
Adailton T. da Silva ◽  
Júlio C.V. Penna ◽  
Luiz R. Goulart ◽  
Maria A. dos Santos ◽  
Neylson E. Arantes
Keyword(s):  
Genetic Variability ◽  
Soybean Cyst Nematode ◽  
Rapd Markers ◽  
Crop Yields ◽  
Heterodera Glycines ◽  
Profile Analysis ◽  
Random Amplified Polymorphic Dna ◽  
Southeastern Brazil ◽  
Slight Variation ◽  
Great Genetic Diversity

Heterodera glycines Ichinohe, the soybean cyst nematode (SCN) was detected in Brazil in 1992 and since then it has been causing losses to Brazilian soybean crop yields. SCN populations have great genetic diversity which makes it difficult to manage this disease. The objectives of this research were to investigate the genetic variation of 16 SCN populations sampled in middle western and southeastern Brazil, utilizing random amplified polymorphic DNA (RAPD) techniques, and to establish useful and specific RAPD markers for SCN race 3. RAPD markers demonstrated genetic variability among and within SCN populations, and they could be used for monitoring nematode population dynamics. The OPA-07 primer was a reliable molecular marker for race 3, while electrophoretic profile analysis of DNA fragments amplified with OPA-10 primer detected slight variation within those populations identified as race 3. The SCN population from Chapadão do Céu, GO - sample 2 - was the most genetically distant from the other populations.

Detection of genetic diversity in tea (Camellia sinensis) using RAPD markers

Genome ◽  
1995 ◽  
Vol 38 (2) ◽  
pp. 201-210 ◽  
Author(s):  
F. N. Wachira ◽  
R. Waugh ◽  
W. Powell ◽  
C. A. Hackett
Keyword(s):  
Genetic Diversity ◽  
Genetic Variability ◽  
Southeast Asia ◽  
Camellia Sinensis ◽  
Rapd Markers ◽  
Rapd Analysis ◽  
Random Amplified Polymorphic Dna ◽  
Systematic Assessment ◽  
Tree Crop ◽  
Taxonomic Relationships

Camellia sinensis is a beverage tree crop native to Southeast Asia and introductions have been made into several nonindigenous countries. No systematic assessment of genetic variability in tea has been done anywhere. In this study, random amplified polymorphic DNA (RAPD) analysis was used to estimate genetic diversity and taxonomic relationships in 38 clones belonging to the three tea varieties, assamica, sinensis, and assamica ssp. lasiocalyx. Extensive genetic variability was detected between species, which was partitioned into between and within population components. Seventy percent of the variation was detected within populations. Analyses based on band sharing separated the three populations in a manner consistent with both the present taxonomy of tea and with the known pedigrees of some clones. RAPD analysis also discriminated all of the 38 commercial clones, even those which cannot be distinguished on the basis of morphological and phenotypic traits.Key words: genetic diversity, RAPDs, Camellia sinensis.

scholarly journals Genetic characterization of the Moxotó goat breed using RAPD markers

2005 ◽  
Vol 40 (3) ◽  
pp. 233-239 ◽  
Author(s):  
Rejane Rodrigues de Oliveira ◽  
Andréa Alves do Egito ◽  
Maria Norma Ribeiro ◽  
Samuel Rezende Paiva ◽  
Maria do Socorro Maués Albuquerque ◽  
...  
Keyword(s):  
Genetic Variability ◽  
Rapd Markers ◽  
Genetic Characterization ◽  
Random Amplified Polymorphic Dna ◽  
Genetic Distances ◽  
Goat Breed ◽  
Breeding Programs ◽  
Analysis Of Molecular Variance ◽  
Polymorphic Bands

The objective of this study was to verify the genetic diversity between and within seven populations of Moxotó goat (n = 264) from the States of Pernambuco, Paraíba and Rio Grande do Norte, using RAPD (Random Amplified Polymorphic DNA). Moxotó, as well as other naturalized breeds, suffers genetic losses due to the indiscriminate miscegenation with breeds raised in the Northeast Region of Brazil. The genetic characterization of these genetic resources is essential to conservation and breeding programs. DNA was extracted from lymphocytes using a non-organic protocol. The 16 primers used were selected from 120 decamer oligonucleotide primers and generated 56 polymorphic bands. The analysis of molecular variance (AMOVA) showed that the greater part of total genetic variability (71.55%) was due to differences between individuals within populations, while 21.21% was among populations. The analysis of variance among the pairs of populations demonstrated that the populations located in Floresta, PE x Angicos, RN presented a smaller value of intrapopulational differentiation (8.9%), indicating low genetic variability among them. Nei's genetic distances varied between 0.0546 and 0.1868 in the populations. The dendrogram generated showed that the Canindé breed, used as outgroup, clustered with the populations of Moxotó, indicating a possible common origin of the naturalized goat breeds.

scholarly journals Isozyme and RAPD studies in Prosopis glandulosa and P. Velutina (Leguminosae, Mimosoideae)

2000 ◽  
Vol 23 (3) ◽  
pp. 639-648 ◽  
Author(s):  
Cecilia Bessega ◽  
Beatriz O. Saidman ◽  
Juan C. Vilardi
Keyword(s):  
Gene Flow ◽  
Genetic Structure ◽  
Genetic Variability ◽  
Genetic Differentiation ◽  
Rapd Markers ◽  
Random Amplified Polymorphic Dna ◽  
Mantel Test ◽  
Prosopis Glandulosa ◽  
Geographical Distance ◽  
Taxonomic Studies

Allozyme and random amplified polymorphic DNA (RAPD) techniques have been compared for their usefulness for genetic and taxonomic studies in Prosopis glandulosa and P. velutina populations. Isozymes and RAPDs yielded similarly high estimates of genetic variability. Genetic structure and differentiation were analyzed through non-hierarchical Wright's F DT. For all populations considered, both markers produced low gene flow (Nm < 1) estimates. When only P. glandulosa populations were analyzed, isozyme data yielded higher gene flow estimates (Nm > 1), in agreement with that expected for conspecific populations. However, in RAPD data the expected reduction in F DT and the increase in Nm were not observed. Correlation between F DT and geographical distance matrices (Mantel test) for all populations was significant (P = 0.02) when based on isozymes, but not so (P = 0.33) when based on RAPDs. No significant associations among genetic and geographical or climatic variables were observed. Two isoenzyme systems (GOT and PRX) enabled us to distinguish between P. glandulosa and P. velutina, but no diagnostic band for recognition of populations or species studied here were detected by RAPD. However, RAPD markers showed higher values for genetic differentiation among conspecific populations of P. glandulosa and a lower coefficient of variation than those obtained from isozymes.

scholarly journals Genetic diversity among forty coffee varieties assessed by RAPD markers associated with restriction digestion

2005 ◽  
Vol 48 (4) ◽  
pp. 511-521 ◽  
Author(s):  
Leandro Eugênio Cardamoni Diniz ◽  
Claudete de Fátima Ruas ◽  
Valdemar de Paula Carvalho ◽  
Fabrício Medeiros Torres ◽  
Eduardo Augusto Ruas ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Genetic Variation ◽  
Genetic Variability ◽  
Clustering Analysis ◽  
Genomic Dna ◽  
Rapd Markers ◽  
Random Amplified Polymorphic Dna ◽  
Pcr Amplification ◽  
Restriction Digestion ◽  
Agronomic Features

The genetic variability of 40 accessions of_C. arabica was evaluated using a combination of the random amplified polymorphic DNA (RAPD) technique and restriction digestion of genomic DNA. The genetic variability and the relatedness among all accessions were initially evaluated using 195 RAPD primers which revealed a very low level of genetic variation. To improve the efficiency in the detection of polymorphism, the genomic DNA of all accessions were submitted to digestion with restriction endonucleases prior to PCR amplification. A total of 24 primers combined with restriction digestion of DNA rendered 318 bands, of which 266 (83.65%) were polymorphic. The associations among genotypes were estimated using UPGMA-clustering analysis. The accessions were properly clustered according to pedigree and agronomic features. The ability to distinguish among coffee accessions was greater for RAPD plus restriction digestion than for RAPD alone, providing evidences that the combination of the techniques was very efficient for the estimation of genetic relationship among_C. arabica genotypes.

Genetic variability and phylogeny inferred from random amplified polymorphic DNA data reflect life strategy of entomopathogenic fungi

2000 ◽  
Vol 78 (9) ◽  
pp. 1150-1155 ◽  
Author(s):  
Miroslav Oborník ◽  
Marek Klíc ◽  
Lukáš Zizka
Keyword(s):  
Genetic Variability ◽  
Entomopathogenic Fungi ◽  
Rapd Markers ◽  
Random Amplified Polymorphic Dna ◽  
Genetic Distances ◽  
Major Influence ◽  
Life Strategy ◽  
Verticillium Lecanii ◽  
Paecilomyces Fumosoroseus ◽  
Neighbor Joining

Our analysis of the genetic variability and phylogenetic relationships among the isolates of mitosporic entomopathogenic fungi Paecilomyces fumosoroseus (Wize) Brown & Smith (10 isolates), Paecilomyces farinosus (Holm ex S.F. Gray) Brown & Smith (3 isolates), Paecilomyces lilacinus (Thom) Samson (1 isolate), and Verticillium lecanii (Zimmerman) Viegas (6 isolates) was based on the use of random amplified polymorphic DNA (RAPD) markers. Neighbor-joining analysis characterized P. fumosoroseus and V. lecanii as monophyletic taxa. The analysis also supported monophyly of the genus Paecilomyces. We have used the distribution of genetic distances to demonstrate the genetic variability within studied species, in Paecilomyces spp. (Hyphomycetes) and Aschersonia spp. (Coelomycetes) in particular. We conclude that the RAPD-based analysis is useful for the reconstruction of intraspecific and interspecific relationships of the entomopathogenic fungi. Moreover, it can be employed to study the intergeneric relationships, especially in the case of closely related genera of the hyphomycetous entomopathogenic fungi. We propose that RAPD-based phylogeny and genetic variability reflect their life strategy. In our opinion, the mobility of spores represents a factor with major influence on the evolution of these entomopathogenic fungi.Key words: Paecilomyces, Verticillium, Aschersonia, RAPD, phylogeny, genetic variability.

scholarly journals Intra and inter populational genetic variability in Maytenus ilicifolia Mart. ex Reiss. 1861, through RAPD markers

2007 ◽  
Vol 67 (4 suppl) ◽  
pp. 957-961 ◽  
Author(s):  
AJ. Mossi ◽  
RL. Cansian ◽  
O. Leontiev-Orlov ◽  
EM. Zanin ◽  
CH. Oliveira ◽  
...  
Keyword(s):  
Genetic Variability ◽  
Dna Markers ◽  
Rapd Markers ◽  
Random Amplified Polymorphic Dna ◽  
Folk Medicine ◽  
And Cluster Analysis ◽  
Genetic Pool ◽  
Maytenus Ilicifolia ◽  
Two Populations ◽  
Anthropic Action

Maytenus ilicifolia is a medicinal plant largely used in the South Brazilian folk medicine. The aim of this study was to quantify the intra and inter populational genetic variability in three populations of M. ilicifolia, focusing on the genetic conservation of this species, which has been threatened by anthropic action. RAPD (Random Amplified Polymorphic DNA) markers were used to analyze 30 plants of each of the three populations collected in the Alto Uruguai Gaúcho region. Fourteen selected primers generated a total of 158 bands, 71.5% of which were polymorphic. The comparison of Jaccard’s distances showed that the intra populational variation was higher than the inter populational variability, and cluster analysis allowed the separation of the three populations. Just 7.6% of the bands were specific of at least two populations. Data indicate that the analyzed M. ilicifolia populations represent a single genetic pool, and therefore any of the population thoroughly can represent the overall genetic variability of the species in the sampled region.

scholarly journals GENETIC VARIABILITY OF THE CEREAL (POACEAE) GERMPLASM COLLECTION MONITORED BY PROTEIN AND MOLECULAR MARKERS

AGROFOR ◽  
2018 ◽  
Vol 2 (1) ◽  
Author(s):  
Miodrag DIMITRIJEVIĆ ◽  
Sofija PETROVIĆ ◽  
Borislav BANJAC ◽  
Goran BARAĆ ◽  
Aleksandra YURIEVNA DRAGOVIĆ ◽  
...  
Keyword(s):  
Genetic Variability ◽  
Rapd Markers ◽  
Allelic Variation ◽  
Random Amplified Polymorphic Dna ◽  
Population Sample ◽  
Germplasm Collection ◽  
Agricultural Practice ◽  
Information Index ◽  
Genotype Variation ◽  
Pilot Investigation

All the new challenges that food production has been experienced, requiresadequate response not only in wide agricultural practice, but also in modernbreeding programs. Broadening genetic variability is indispensable to meeting theborder set by climatic changes, land erosion, human population growth, andsustainable agriculture. Gathering genetic variability, forming and examininggenetic collection are integral part of the task. A germplasm collection of 220entries, consisting of cereal (Poaceae) genotypes has been formed. Geneticvariability of wheat (Triticum sp. ), barley (Hordeum sp. ), and goat grass (Aegilopssp. ), samples from the germplasm collection was analysed using gliadin blocks asprotein markers, as well as, Random Amplified Polymorphic DNA (RAPD)markers. Gliadin allelic variation was notable within Triticum sp. samples,revealing not only genetic divergence, but also the origin and the structure ofpopulations. Genotype variation and structure of populations of Hordeum sp. wasfollowed by hordein allelic variation. Sampled population expressed heterogeneityfrom two to five genotypes per population sample. Landraces, old and modernvarieties were separated in collection using hordein allelic variation, as well. Asmall, pilot, investigation was conducted on Aegilops sp. polymorphism usingRAPD primers. Number and percentage of polymorphic loci, effective number ofalleles, expected heterozygosity and Shannon’s information index were used toestimate genetic variation.

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