scholarly journals Estimation of endogenous contribution and urinary excretion of purine derivatives from the total digestible nutrient intake in Nellore heifers

2012 ◽  
Vol 41 (8) ◽  
pp. 1899-1906 ◽  
Author(s):  
Jercyane Maria da Silva Braga ◽  
Rilene Ferreira Diniz Valadares ◽  
Samantha Gusmão Pellizzoni ◽  
Sebastião de Campos Valadares Filho ◽  
Luciana Louzada Prates ◽  
...  
2013 ◽  
Vol 112 (1-3) ◽  
pp. 49-55 ◽  
Author(s):  
Tao Ma ◽  
Kaidong Deng ◽  
Chenggang Jiang ◽  
Yan Tu ◽  
Naifeng Zhang ◽  
...  

2003 ◽  
Vol 140 (1) ◽  
pp. 101-105 ◽  
Author(s):  
T. FUJIHARA ◽  
M. TODOROKI ◽  
K. NAKAMURA

Urinary purine derivative (PD) excretion was estimated to examine the effect of rumen protozoa on total PD excretion in goats fed hay and a concentrate diet. The effect of increasing protozoa number in the rumen on nitrogen (N) balance and urinary PD excretion was determined after inoculation. Protozoa increased slowly until 4 days after inoculation, and on the 5th day after inoculation rapidly, finally (10 days) reaching 4·1×105/ml of rumen contents similar to that before defaunation. Urinary N excretion showed a small (non-significant) decrease. Urinary PD excretion did not change until the 7th day, and then the level decreased on the 8th day after faunation presumably due to the effect of increased protozoa in the rumen. The mean urinary total PD excretion significantly (P<0·05) decreased in the defaunated group compared with that in the faunated group. Comparable changes were not seen in plasma PD level of faunated and defaunated groups.


Author(s):  
X. B. Chen ◽  
Adriana T. Mejia ◽  
D. J. Kyle ◽  
E. R. Ørskov

In ruminants, daily urinary excretion of purine derivatives (PD) reflects the absorption of microbial purines and can be used as an index of microbial protein supply (Chen, Ørskov and Hovell, 1991). The application could be extended to farm conditions if measurements based on spot urine samples or plasma could serve as an alternative index. The objective of this study was to examine whether PD concentrations in spot urine or plasma samples vary diurnally during a given feeding regime and if they reflect differences in daily PD excretion induced by varying feed intake.


1998 ◽  
Vol 1998 ◽  
pp. 80-80
Author(s):  
F. Herrera Gomez ◽  
F. D. Deb Hovell ◽  
C. A. Sandoval Castro

Studies in the use of the purine derivatives technique in ruminants have been stimulated by the possible use of this technique as an estimator of the rumen microbial-N supplied to the host animal. The recovery factor influences the estimation of the total purines absorbed and therefore the microbial-N supply. The relationship between exogenous purine input and urinary excretion and recovery has been studied using cattle maintained with the intragastric infusion technique (Orskov et al., 1979). The urinary recovery of exogenous purines has been estimated to be 0.77-0.85 (Chen et al., 1990a, Verbic et al., 1990), and this relationship has been assumed to be applicable to normal feeding situations. To our knowledge there is no data to support or reject this approach. This study examined the urinary recovery of exogenous allantoin input in steers under normal feeding conditions.


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