Metformin synergistically enhances antiproliferative effects of cisplatin and etoposide in NCI-H460 human lung cancer cells

OBJECTIVE: To test the effectiveness of combining conventional antineoplastic drugs (cisplatin and etoposide) with metformin in the treatment of non-small cell lung cancer in the NCI-H460 cell line, in order to develop new therapeutic options with high efficacy and low toxicity.METHODS: We used the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay and calculated the combination index for the drugs studied.RESULTS: We found that the use of metformin as monotherapy reduced the metabolic viability of the cell line studied. Combining metformin with cisplatin or etoposide produced a synergistic effect and was more effective than was the use of cisplatin or etoposide as monotherapy.CONCLUSIONS: Metformin, due to its independent effects on liver kinase B1, had antiproliferative effects on the NCI-H460 cell line. When metformin was combined with cisplatin or etoposide, the cell death rate was even higher.

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Antiproliferative Effects of a Triterpene-Enriched Extract from Lingzhi or Reishi Medicinal Mushroom, Ganoderma lucidum (Agaricomycetes), on Human Lung Cancer Cells

International Journal of Medicinal Mushrooms ◽

10.1615/intjmedmushrooms.2018028823 ◽

2018 ◽

Vol 20 (12) ◽

pp. 1173-1183 ◽

Cited By ~ 6

Author(s):

Sanda Zolj ◽

Melissa P. Smith ◽

Jillian C. Goines ◽

T'Shura S. Ali ◽

Mary O. Huff ◽

...

Keyword(s):

Lung Cancer ◽

Cancer Cells ◽

Ganoderma Lucidum ◽

Human Lung ◽

Lung Cancer Cells ◽

Human Lung Cancer ◽

Medicinal Mushroom ◽

Antiproliferative Effects ◽

Human Lung Cancer Cells

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Suppressive oligodeoxynucleotides synergistically enhance antiproliferative effects of anticancer drugs in A549 human lung cancer cells

International Journal of Oncology ◽

10.3892/ijo.2012.1755 ◽

2012 ◽

Vol 42 (2) ◽

pp. 429-436 ◽

Cited By ~ 2

Author(s):

RYOHEI TAKAHASHI ◽

TAKASHI SATO ◽

DENNIS M. KLINMAN ◽

TAKESHI SHIMOSATO ◽

TAKESHI KANEKO ◽

...

Keyword(s):

Lung Cancer ◽

Cancer Cells ◽

Anticancer Drugs ◽

Human Lung ◽

Lung Cancer Cells ◽

Human Lung Cancer ◽

Antiproliferative Effects ◽

Human Lung Cancer Cells

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Fucosterol exerts antiproliferative effects on human lung cancer cells by inducing apoptosis, cell cycle arrest and targeting of Raf/MEK/ERK signalling pathway

Phytomedicine ◽

10.1016/j.phymed.2018.12.032 ◽

2019 ◽

Vol 61 ◽

pp. 152809 ◽

Cited By ~ 9

Author(s):

Zhangfan Mao ◽

Xiaoling Shen ◽

Ping Dong ◽

Gaoli Liu ◽

Shize Pan ◽

...

Keyword(s):

Lung Cancer ◽

Cell Cycle ◽

Cell Cycle Arrest ◽

Cancer Cells ◽

Human Lung ◽

Lung Cancer Cells ◽

Human Lung Cancer ◽

Antiproliferative Effects ◽

Cycle Arrest ◽

Human Lung Cancer Cells

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The Extract of Hibiscus syriacus Inducing Apoptosis by Activating p53 and AIF in Human Lung Cancer Cells

The American Journal of Chinese Medicine ◽

10.1142/s0192415x08005680 ◽

2008 ◽

Vol 36 (01) ◽

pp. 171-184 ◽

Cited By ~ 28

Author(s):

Yeung-Leung Cheng ◽

Shih-Chun Lee ◽

Horng-Jyh Harn ◽

Hsin-Chieh Huang ◽

Wen-Liang Chang

Keyword(s):

Lung Cancer ◽

Cell Carcinoma ◽

Cancer Cells ◽

Human Lung ◽

Lung Cancer Cells ◽

Human Lung Cancer ◽

Antiproliferative Effects ◽

Human Lung Cancer Cells ◽

Hibiscus Syriacus

Natural products including plants, microorganisms and marine life provide rich resources for anticancer drug discovery. The root bark of Hibiscus syriacus has been used as an antipyretic, anthelmintic and antifungal agent in Asia. The antiproliferative effects of H. syriacus on human lung cancer cells were evaluated with bio-assays. The apoptotic activity was detected by Hoechst 33342 DNA staining and annexin V staining. The expression of caspases, p53, apoptosis induced factor (AIF), Bcl-2 and Bax were evaluated with Western blotting. The in vivo anticancer activity was evaluated using A549-xenograft model. The acetone extract of H. syriacus (HS-AE) exhibited a better cytotoxic effect on lung cancer cells than its methanol extract (HS-ME) or water extract (HS-WE). The IC50 values of HS-AE on A549 (adenocarcinoma), H209 (squamous cell carcinoma) or H661 (large cell carcinoma) lung cancer cells ranged from 14 to 22 μg/ml after 48 hours of treatment. After 48 hours of exposure, HS-AE (15 μg/ml) induced A549 cell apoptosis to 48 ± 3.6% of the control. Using Western blotting, HS-AE appears to suppress the expression of p53 and AIF. The results of the in vivo study showed that HS-AE suppresses growth in A549 subcutaneous xenograft tumors. These results indicate that HS-AE exerts significant and dose-dependent antiproliferative effects on cancer cells in vitro and in vivo, which prompts us to further evaluate and elucidate the bioactive component(s) of H. syriacus.

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Superior antiproliferative effects mediated by interferon-α entrapped in liposomes against a newly established human lung cancer cell line

Lung Cancer ◽

10.1016/0169-5002(91)90130-x ◽

1991 ◽

Vol 7 (3) ◽

pp. 188

Keyword(s):

Lung Cancer ◽

Cell Line ◽

Cancer Cell ◽

Human Lung ◽

Cancer Cell Line ◽

Lung Cancer Cell Line ◽

Human Lung Cancer ◽

Interferon Α ◽

Lung Cancer Cell ◽

Antiproliferative Effects

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Anticancer activity of peptide extracted from edible mushroom; Lentinus squarrosulus in human lung cancer cells

Planta Medica ◽

10.1055/s-0036-1596829 ◽

2016 ◽

Vol 81 (S 01) ◽

pp. S1-S381

Author(s):

S Sumkhemthong ◽

M Suksomtip ◽

P Chanvorachote ◽

C Chaotham

Keyword(s):

Lung Cancer ◽

Anticancer Activity ◽

Cancer Cells ◽

Human Lung ◽

Edible Mushroom ◽

Lung Cancer Cells ◽

Human Lung Cancer ◽

Human Lung Cancer Cells

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Inhibitory effects of Actinidia Chinensis planch root extracts (acRoots) on human lung cancer cells through retinoic acid receptor beta

Molecular and Cellular Therapies ◽

10.26781/2052-8426-2017-02 ◽

2017 ◽

Vol 5 (1) ◽

Cited By ~ 1

Author(s):

Lingyan Wang ◽

Jiayun Hou ◽

Minghuan Zheng ◽

Lin Shi

Keyword(s):

Lung Cancer ◽

Cancer Cells ◽

Human Lung ◽

Retinoic Acid Receptor ◽

Lung Cancer Cells ◽

Human Lung Cancer ◽

Inhibitory Effects ◽

The Core ◽

Human Lung Cancer Cells ◽

Receptor Beta

Actinidia Chinensis Planch roots (acRoots) are used to treat many cancers, although the anti-tumor mechanism by which acRoots inhibit cancer cell growth remains unclear. The present study aims at investigating inhibitory effects of acRoots on human lung cancer cells and potential mechanisms. Our data demonstrate that the inhibitory effects of acRoots on lung cancer cells depend on genetic backgrounds and phenotypes of cells. We furthermore found the expression of metabolism-associated gene profiles varied between acRoots-hypersensitive (H460) or hyposensitive lung cancer cells (H1299) after screening lung cancer cells with different genetic backgrounds. We selected retinoic acid receptor beta (RARB) as the core target within metabolism-associated core gene networks and evaluated RARB changes and roles in cells treated with acRoots at different concentrations and timeframes. Hypersensitive cancer cells with the deletion of RARB expression did not response to the treatment with acRoots, while RARB deletion did not change effects of acRoots on hyposensitive cells. Thus, it seems that RARB as the core target within metabolism-associated networks plays important roles in the regulation of lung cancer cell sensitivity to acRoots.

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