scholarly journals HPV in oral squamous cell carcinomas of a Brazilian population: amplification by PCR

2006 ◽  
Vol 20 (1) ◽  
pp. 21-24 ◽  
Author(s):  
Elena Riet Correa Rivero ◽  
Fabio Daumas Nunes
Keyword(s):  
Squamous Cell ◽  
Pcr Amplification ◽  
Dna Amplification ◽  
Hpv Infection ◽  
Positive Control ◽  
Squamous Cell Carcinomas ◽  
Hpv 16 ◽  
Oral Squamous Cell Carcinomas ◽  
Hpv Dna ◽  
High Risk Hpv

Human Papilomaviruses (HPV) are a group of viruses associated with benign and malignant lesions of cutaneous and mucosal epithelia. Some "high risk" HPV types, especially HPV 16 and 18, are strongly correlated with cervical and anogenital cancers and are also related to the genesis of oral squamous cell carcinomas (OSCC). The aim of this work was to investigate the incidence of HPV infection in 40 paraffin-embedded or fresh specimens of OSCC, using PCR amplification of the viral DNA. Literature based primers (GP5+/GP6+) were used in order to amplify HPV DNA from the L1 gene, present in more than 22 types of HPV. A condyloma case with HPV 16 and 18 detected by in situ hybridization was used as a positive control. Amplification of HPV was observed only in the positive control. No squamous cell carcinoma cases showed DNA viral amplification. Absence of HPV DNA amplification by PCR in the analyzed specimens of OSCCs suggests that this virus not always plays a role in the carcinogenesis process. Discrepancy with some studies found in the literature may be related to methodology or population differences.

Genetic polymorphisms and HPV infection in oral squamous cell carcinomas

2015 ◽  
Vol 14 ◽  
pp. 1-6 ◽  
Author(s):  
Yan Sun ◽  
Yang Zhang ◽  
Limei Liu ◽  
Xicheng Song ◽  
Guojun Li
Keyword(s):  
Genetic Polymorphisms ◽  
Squamous Cell ◽  
Hpv Infection ◽  
Squamous Cell Carcinomas ◽  
Oral Squamous Cell Carcinomas

scholarly journals Prevalence of Human Papillomavirus Associated with Head and Neck Squamous Cell Carcinoma in Jordanian Patients

2020 ◽  
Vol 14 (1) ◽  
pp. 57-64
Author(s):  
Ashraf I. Khasawneh ◽  
Nisreen Himsawi ◽  
Jumana Abu-Raideh ◽  
Muna Salameh ◽  
Niveen Abdullah ◽  
...  
Keyword(s):  
Squamous Cell Carcinoma ◽  
Human Papillomavirus ◽  
Cell Carcinoma ◽  
Head And Neck ◽  
Squamous Cell ◽  
Hpv Infection ◽  
Neck Squamous Cell Carcinoma ◽  
Hpv 16 ◽  
Hpv Dna ◽  
Prophylactic Measures

Background: In addition to smoking and alcohol consumption, human papillomavirus (HPV) is a leading etiology for Head and Neck Squamous Cell Carcinoma (HNSCC). However, this causal association is still understudied in Middle Eastern populations. Objective: The aim of this study was to determine the prevalence of HPV-associated infection in the Jordanian HNSCC patients and the associated HPV genotypes. Methods: Formalin-Fixed Paraffin-Embedded (FFPE) squamous cell carcinoma samples of the head and neck were collected from two referral centers in Amman, Jordan to determine the existence of HPV DNA. After DNA extraction HPV infection and genotyping were identified using real-time PCR. Results: HPV DNA was detected in 19 out of 61 (31.1%) HNSCC samples. Despite screening for 28 different genotypes, HPV 16 was the only genotype identified in all examined samples. Most HPV-positive samples were obtained from the oropharynx (41.7%), oral cavity (37%), and larynx (18.2%). No significant association between HPV 16 genotype and age, sex, tobacco use, anatomical location, or tumor grade was noticed. Conclusion: This study reported a high association between HPV 16 genotype and HNSCC in Jordanian patients. These data should facilitate the implementation of appropriate HPV awareness campaigns, and activate selective prophylactic measures against HPV infection.

Clinical Analysis of Human Papillomavirus Infection Types Distribution in Tissues of Cervical Squamous Cell Carcinoma

2020 ◽  
Vol 12 (7) ◽  
pp. 921-927
Author(s):  
Xiumei Zhang ◽  
Xianhai Zhu ◽  
Xiaolan Xu ◽  
Chengfu Sun ◽  
Jianxiang Geng ◽  
...  
Keyword(s):  
Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Infection Rate ◽  
Squamous Cell ◽  
Eastern China ◽  
Cervical Squamous Cell Carcinoma ◽  
Hpv Infection ◽  
Squamous Cell Carcinomas ◽  
Hpv 16 ◽  
Tissue Samples

In this article, we investigated prevalence and distributed types of human papillomavirus (HPV) among female cervical squamous cell carcinoma patients in eastern China (mainly in Jiangsu Province). Tissue samples of total 1021 cases with cervical squamous cell carcinoma were incurred from female patients passing through biopsy or surgery. HPV DNA and genotypes of all subjects were examined using polymerase chain reaction (PCR) and gene-chip. There were 937 positive cases among 1021 cervical squamous cell carcinoma tissue samples with the total HPV infection rate of 91.77%. The HPV infection rate of single type was 75.42%. The predominant types of single infection with HPV were HPV 16 (52.60%), HPV 18 (5.19%), HPV 58 (4.51%), HPV 52 (2.84%), HPV 31 (2.74%), HPV 33 (2.45%), HPV 59 (1.76%) and HPV 45 (0.88%). The HPV infection rate of multiple types was 16.35%. The predominant types of multiple infection with HPV were HPV 16+18 (19.16%), 16+58 (6.59%),16+52 (5.99%), 16+33 (5.39%), 11+16 (2.99%), 16+31 (2.99%), 16+45 (2.99%), 16+42 (2.40%) and 16+59 (2.40%). Cervical squamous cell carcinomas had a close relationship with HPV infection. HPV 16, 18, 58, 52, 31, 33, 59, 45 with high prevalence in the cervical squamous cell carcinoma deserve great attention in eastern China (mainly in Jiangsu Province). The protection spectrum of divalent vaccine and ninevalent vaccine were, respectively, 68.52% (642/937) and 90.82% (851/937) in the cases of cervical squamous cell carcinomas.

Immunohistochemical Localization of Cdc6 in Squamous and Glandular Neoplasia of the Uterine Cervix

2002 ◽  
Vol 126 (10) ◽  
pp. 1164-1168
Author(s):  
Lian Bonds ◽  
Paige Baker ◽  
Carol Gup ◽  
Kenneth R. Shroyer
Keyword(s):  
Squamous Cell ◽  
Cellular Proliferation ◽  
Hpv Infection ◽  
Squamous Cell Carcinomas ◽  
Adenocarcinoma In Situ ◽  
Immunoperoxidase Method ◽  
High Grade ◽  
Hpv Dna ◽  
Mib 1

Abstract Context.—Cdc6 has been extensively studied as a marker for cellular proliferation that is expressed during the normal cell cycle. Recent studies indicate that Cdc6 may be a marker for cervical intraepithelial neoplasia (CIN) and carcinoma; however, the histologic distribution of Cdc6 has not been explicitly defined. Expression of Cdc6 in the endocervical mucosa also remains unexplored. Objective.—The goal of the current study was to evaluate the distribution of Cdc6 protein, MIB-1 protein, and human papillomavirus (HPV) DNA in a broad range of cervical tissues, including normal, potentially premalignant, and malignant lesions of the ectocervical and endocervical mucosa. Methods.—We used an indirect immunoperoxidase method to stain formalin-fixed, paraffin-embedded tissues and frozen tissues, including biopsy and hysterectomy specimens, for Cdc6 and MIB-1 proteins, and we used in situ hybridization to detect HPV DNA in a subset of cases. Results.—Cdc6 staining was exclusively nuclear and was present in both squamous and glandular epithelial cells of histologic sections. Cdc6 staining was rarely present in specimens of normal cervical squamous mucosa (2/84, 2.4%) or in specimens with squamous metaplasia (3/59, 5.1%) and was not detected in normal endocervical glands (0/84). Staining was present in most cases of CIN I (31/48, 65%). Staining was present in the majority of cases of CIN II (25/28, 89%) and in all cases of CIN III (36/36) and squamous cell carcinomas (34/34). The proportion of cells staining for Cdc6 increased with the grade of dysplasia, and the proportion of stained cells in squamous cell carcinomas was similar to that in lesions of high-grade dysplasia. Cdc6 staining was present in the majority of cases in glandular lesions including adenocarcinoma in situ (11/14, 79%) and adenocarcinoma (8/10, 80%). The histologic distribution of Cdc6-immunoreactive cells was similar to that of cells with a strong signal for HPV DNA, but Cdc6 protein and HPV DNA did not colocalize at the level of individual cells. Conclusion.—Cdc6 expression is a marker for high-grade cervical squamous and glandular dysplasia and carcinoma and is associated with HPV infection. The mechanistic basis of the association between HPV infection and Cdc6 immunopositivity remains to be determined but may represent either up-regulation of Cdc6 expression or stabilization of the Cdc6 protein.

Nuclear Expression of B-Catenin in Oral Squamous Cell Carcinomas Infected By HPV 16/18

2015 ◽  
Vol 119 (3) ◽  
pp. e130
Author(s):  
Montserrat Reyes ◽  
Gonzalo Rojas ◽  
Ana Verónica Ortega ◽  
Andrea Maturana ◽  
Juan Pablo Aitken ◽  
...  
Keyword(s):  
Squamous Cell ◽  
Squamous Cell Carcinomas ◽  
Hpv 16 ◽  
Oral Squamous Cell Carcinomas ◽  
Nuclear Expression
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