scholarly journals In vitro activity of Artemisia annua L (Asteraceae) extracts against Rhipicephalus (Boophilus) microplus

2011 ◽  
Vol 20 (1) ◽  
pp. 31-35 ◽  
Author(s):  
Ana Carolina de Souza Chagas ◽  
Cynthia Sanches Georgetti ◽  
Camila Olivo de Carvalho ◽  
Márcia Cristina de Sena Oliveira ◽  
Rodney Alexandre Rodrigues ◽  
...  

The activity of plant extracts on parasites may indicate groups of substances that are potentially useful for controlling Rhipicephalus (Boophilus) microplus. The aim of the present study was to investigate the in vitro action of Artemisia annua extracts on this tick. The concentrations of the sesquiterpene lactones artemisinin and deoxyartemisinin present in plant extracts were quantified via high-performance liquid chromatography. Four extracts produced from the concentrated crude extract (CCE) were evaluated on larvae using the impregnated paper method, with readings after 24 hours of incubation. The engorged females were immersed in the CCE and in its four derived extracts for five minutes, with incubation for subsequent analysis of biological parameters. The extracts were not effective on the larvae at the concentrations tested (3.1 to 50 mg.mL-1). The CCE showed greater efficacy on engorged females (EC50 of 130.6 mg.mL-1 and EC90 of 302.9 mg.mL-1) than did the derived extracts. These results tend to confirm that the action of artemisinin on engorged females of R.(B.) microplus is conditional to their blood intake. In this case, in vitro methods would be inadequate for effective evaluation of the action of A. annua on R. (B.) microplus.

2017 ◽  
Vol 27 (1) ◽  
pp. 70-76 ◽  
Author(s):  
Caroline V.V. Castilho ◽  
Rafaela R. Fantatto ◽  
Yousmel A. Gaínza ◽  
Humberto R. Bizzo ◽  
Nancy S. Barbi ◽  
...  

2018 ◽  
Vol 84 (0) ◽  
Author(s):  
Eduardo Oliveira ◽  
Manoela da Silva ◽  
Lew Sprenger ◽  
Daniela Pedrassani

ABSTRACT: Rhipicephalus (Boophilus) microplus, known as the cattle tick, is a cause of great economic losses for dairy cattle farming because of its high frequency of occurrence and the difficulty in controlling it. The aim of this study was to evaluate the in vitro activity of Chenopodium ambrosioides extract on R. (B.) microplus. For this purpose, 125 females were selected and classified into five groups according to their weight, in order to ensure that the females used presented homogeneous weight. The treatments comprised 40 and 60% extracts of Chenopodium ambrosioides, distilled water, ethanol (70ºGL) and 12.5% amitraz. The extracts of C. ambrosioides (40 and 60%) showed effectiveness of 99.7 and 100% and higher percentages of dead females than the other treatments: 64 and 96%, respectively (p<0.001). In the groups exposed to distilled water and ethanol (70º GL), 92 and 88% of the females maintained oviposition. In the females exposed to 40 and 60% extracts, oviposition of 36 and 4% occurred, respectively. It was concluded that the extract of Chenopodium ambrosioides, at both concentration evaluated, had high efficiency against engorged females of cattle ticks.


1997 ◽  
Vol 41 (1) ◽  
pp. 215-217 ◽  
Author(s):  
K Ingolfsdottir ◽  
M A Hjalmarsdottir ◽  
A Sigurdsson ◽  
G A Gudjonsdottir ◽  
A Brynjolfsdottir ◽  
...  

With reference to the traditional use of Cetraria islandica (Iceland moss) for relief of gastric and duodenal ulcer, plant extracts were screened for in vitro activity against Helicobacter pylori. (+)-Protolichesterinic acid, an aliphatic alpha-methylene-gamma-lactone, was identified as an active component. The MIC range of protolichesterinic acid, in free as well as salt form, was 16 to 64 micrograms/ml.


2011 ◽  
Vol 110 (1) ◽  
pp. 295-303 ◽  
Author(s):  
Ana Carolina de Souza Chagas ◽  
Luiz Daniel de Barros ◽  
Fernando Cotinguiba ◽  
Maysa Furlan ◽  
Rodrigo Giglioti ◽  
...  

2017 ◽  
Vol 11 (06) ◽  
pp. 683-689 ◽  
Author(s):  
James W. Muthomi ◽  
◽  
Geraldin M. W. Lengai ◽  
Maina J. Wagacha ◽  
Rama D. Narla ◽  
...  

2021 ◽  
Vol 18 (1) ◽  
Author(s):  
Chuanxiong Nie ◽  
Jakob Trimpert ◽  
Sooyeon Moon ◽  
Rainer Haag ◽  
Kerry Gilmore ◽  
...  

Abstract Background Traditional medicines based on herbal extracts have been proposed as affordable treatments for patients suffering from coronavirus disease 2019 (COVID-19) caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Teas and drinks containing extracts of Artemisia annua and Artemisia afra have been widely used in Africa in efforts to prevent SARS-CoV-2 infection and fight COVID-19. Methods The plant extracts and Covid-Organics drink produced in Madagascar were tested for plaque reduction using both feline coronavirus and SARS-CoV-2 in vitro. Their cytotoxicities were also investigated. Results Several extracts as well as Covid-Organics inhibited SARS-CoV-2 and FCoV infection at concentrations that did not affect cell viability. Conclusions Some plant extracts show inhibitory activity against FCoV and SARS-CoV-2. However, it remains unclear whether peak plasma concentrations in humans can reach levels needed to inhibit viral infection following consumption of teas or Covid-Organics. Clinical studies are required to evaluate the utility of these drinks for COVID-19 prevention or treatment of patients.


2014 ◽  
Vol 45 (3) ◽  
pp. 769-779 ◽  
Author(s):  
Adriana Lígia de Castilho ◽  
Juliana Paola Correa da Silva ◽  
Cintia Helena Coury Saraceni ◽  
Ingrit Elida Collantes Díaz ◽  
Mateus Luís Barradas Paciencia ◽  
...  

Molecules ◽  
2011 ◽  
Vol 16 (7) ◽  
pp. 5453-5459 ◽  
Author(s):  
Guo-Ying Zuo ◽  
Fan-Yan Meng ◽  
Jun Han ◽  
Xiao-Yan Hao ◽  
Gen-Chun Wang ◽  
...  

Sign in / Sign up

Export Citation Format

Share Document