Hepatic coccidiosis in Serrasalmus rhombeus Linnaeus, 1766 from the Amazon basin: morphological and histopathological aspects

Abstract Calyptospora species are coccids that commonly cause liver infections in fish all around the world. This paper describes the morphology and histopathological characteristics of liver infection caused by Calyptospora sp. in black piranha Serrasalmus rhombeus, from the Capim River, in the municipality of Ipixuna do Pará, state of Pará (Brazil). Specimens were collected, analyzed and necropsied and tissue fragments containing parasites were prepared for histology and scanning electron microscopy. Parasitism was detected in 33.0% of the analyzed specimens, which had spherical oocysts in the liver, with four pyriform sporocysts presenting sporozoites internally. A histological examination revealed oocysts positioned close to blood vessels, causing necrosis and degeneration of hepatic parenchyma, while the presence of mononuclear cell infiltrate and melanomacrophages indicated the onset of an inflammatory process. This is the first record of the genus Calyptospora in fish from the Capim River.

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The first record of the species Lacinius erinaceus Staręga, 1966 (Opiliones, Phalangiidae) in Turkey with some SEM studies on its morphology

Archives of Biological Sciences ◽

10.2298/abs1202659k ◽

2012 ◽

Vol 64 (2) ◽

pp. 659-665 ◽

Cited By ~ 3

Author(s):

K. Kurt ◽

Ö.K. Erman

Keyword(s):

Electron Microscopy ◽

Scanning Electron Microscopy ◽

First Record ◽

The World ◽

Characteristic Features ◽

First Time ◽

Scanning Electron ◽

Sem Studies

The harvestman species of Lacinius erinaceus Star?ga 1966, recorded for the first time in Turkey, is presented in this paper. The characteristic features of this species are described and illustrated and data regarding collecting sites and distribution all over the world are given. Scanning electron microscopy (SEM) studies on the dorsal integument, dorsal habitus, chelicerae, pedipalpus, trident, the ocularium and legs of the specimens are also presented. Localities of the collecting sites are plotted on a map.

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Analysis of the type material of Achnanthes minutissima var. macrocephala (Bacillariophyta) and description of two new small capitate Achnanthidium species from Europe and the Himalaya

Plant Ecology and Evolution ◽

10.5091/plecevo.2019.1628 ◽

2019 ◽

Vol 152 (2) ◽

pp. 340-350

Author(s):

Carlos E. Wetzel ◽

Ingrid Jüttner ◽

Smriti Gurung ◽

Luc Ector

Keyword(s):

Electron Microscopy ◽

Scanning Electron Microscopy ◽

Benthic Diatom ◽

Original Material ◽

Diatom Species ◽

The World ◽

Freshwater Habitats ◽

The Himalaya ◽

Scanning Electron ◽

Achnanthes Minutissima

Background and aims – Two unknown benthic diatom species belonging to the genus Achnanthidium Kütz., and found in French and Nepalese freshwater habitats, were investigated. Both species are here described as new and compared with the original material of Achnanthes minutissima var. macrocephala Hust. [≡ Achnanthidium macrocephalum (Hust.) Round & Bukht.] from Indonesia. Methods – The morphology of three small and capitate Achnanthidium species was investigated using light microscopy (LM) and scanning electron microscopy (SEM). Key results – Achnanthidium coxianum sp. nov. (from the Himalaya) belongs to the group of species with hooked terminal raphe endings, while Achnanthidium peetersianum sp. nov. (from France) has variable terminal raphe endings which are usually slightly bent. Achnanthidium macrocephalum is a much rarer species and illustrations concerning its identity in the literature do not conform to the type studied here. Conclusions – The three species are similar in LM but clearly distinct in SEM. The shape of the areolae and terminal raphe endings separate the species. Achnanthidium macrocephalum has often been misidentified in studies from many areas of the world. It is similar to the new species in valve outline and in its small dimensions.

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Four new Luticola taxa (Bacillariophyta) from Maritime Antarctica

Phytotaxa ◽

10.11646/phytotaxa.170.3.2 ◽

2014 ◽

Vol 170 (3) ◽

pp. 155 ◽

Cited By ~ 3

Author(s):

RALITSA ZIDAROVA ◽

ZLATKO LEVKOV ◽

BART VAN DE VIJVER

Keyword(s):

Electron Microscopy ◽

Scanning Electron Microscopy ◽

New Taxa ◽

Similar Species ◽

The South ◽

Maritime Antarctica ◽

World Data ◽

The World ◽

Scanning Electron

The paper describes four new Luticola taxa from the South Shetland Archipelago, Antarctica: Luticola neglecta sp. nov., L. bogaertsiana sp. nov., L. contii sp. nov. and L. olegsakharovii sp. nov. All new taxa are studied using both light and scanning electron microscopy and compared to other morphologically similar species from (sub-)Antarctica and elsewhere in the world. Data on their ecology and biogeography are added.

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Trachelomonas volzii vs T. dubia (Euglenophyceae)—one or two separate species? Study on similarities and differences of the species

Phytotaxa ◽

10.11646/phytotaxa.357.1.1 ◽

2018 ◽

Vol 357 (1) ◽

pp. 1 ◽

Cited By ~ 1

Author(s):

MAŁGORZATA PONIEWOZIK ◽

KONRAD WOŁOWSKI ◽

JOLANTA PIĄTEK

Keyword(s):

Electron Microscopy ◽

The Other ◽

New Combinations ◽

Separate Species ◽

Phenotypic Changes ◽

The World ◽

Key Features ◽

Similarities And Differences ◽

Species Study ◽

Scanning Electron

This study concerns the two species Trachelomonas volzii and T. dubia which were examined for similarities and dissimilarities of their loricae and monads organization. We specifically focused on the key features of both species that were originally used to separate one from the other: annular thickening at the base of collar and dimensions and ornamentation of loricae. Loricae of specimens were examined by light and scanning electron microscopy and the results were compared with the literature data describing these taxa and reported occurrences in the world. The species together with their varieties and forms do not appear to have strong characteristics distinguishing them, rather the variability observed fits natural phenotypic changes. Based on evidence from this study, we recommend combining these two taxa and propose T. volzii as the single taxon. We also examined a set of varieties of original T. volzii since the species contained several varieties that were almost identical in relation to lorica structure and occurrence. As a result of these observations, we propose the following varieties: Trachelomonas volzii var. volzii as a nominative variety, T. volzii var. australis, T. volzii var. sulcata, T. volzii var. inflata, T. volzii var. acidophila. Furthermore, we propose reclassifying some taxa and the new combinations such as: T. dubia var. ornata to T. volzii var. ornata and T. dubia var. colliundulata to T. volzii var. colliundulata. In our opinion, T. dubia fo. acuminata should be included with the species T. hexangulata due to its unique, hexagonal shaped lorica.

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Comparison of two new species of astogloia Bacillariophyceae) with other small members of section Ellipticae

Phytotaxa ◽

10.11646/phytotaxa.126.1.1 ◽

2013 ◽

Vol 126 (1) ◽

pp. 1 ◽

Cited By ~ 9

Author(s):

CHIARA PENNESI ◽

MICHEL POULIN ◽

FRIEDEL HINZ ◽

TIZIANA ROMAGNOLI ◽

MARIO DE STEFANO ◽

...

Keyword(s):

Electron Microscopy ◽

Scanning Electron Microscopy ◽

New Species ◽

Geographical Distribution ◽

New Taxa ◽

Related Species ◽

The World ◽

Two New Species ◽

Scanning Electron

In this study, seven Mastogloia species belonging of the section Ellipticae are morphologically described through scanning electron microscopy, including two new taxa M. matthaei and M. stellae. They were collected as epiphytes on seagrasses from several tropical (Indonesia), subtropical (Egypt, Greece, Republic of Malta, Turkey) and temperate (Slovenia, Italy) regions of the world. All these species show typical characters of the Hustedt’s section Ellipticae: elliptical valve outline, a flat valve surface and rounded apices. Moreover, this study provides novel information on the frustule ultrastructure and gives update of their current geographical distribution. Some nomenclatural inconsistencies have been resolved with the typification of M. ovulum and the related species, M. emarginata, from the original Miang Besar material in the Hustedt collection.

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Early development of Astronotus ocellatus under stereomicroscopy and scanning electron microscopy

Zygote ◽

10.1017/s0967199411000116 ◽

2011 ◽

Vol 20 (3) ◽

pp. 269-276 ◽

Cited By ~ 6

Author(s):

Maria do Carmo Faria Paes ◽

Lilian Cristina Makino ◽

Leonardo Avendaño Vasquez ◽

João Batista Kochenborger Fernandes ◽

Laura Satiko Okada Nakaghi

Keyword(s):

Electron Microscopy ◽

Scanning Electron Microscopy ◽

Amazon Basin ◽

Cichlid Fish ◽

Fish Farming ◽

Average Temperature ◽

Astronotus Ocellatus ◽

Yolk Absorption ◽

Otic Vesicles ◽

Scanning Electron

SummaryAstronotus ocellatus, popularly known as Oscar, is a cichlid fish from the Amazon basin (Brazil) with a great potential for fish farming. The aim of this research is to describe the morphology of eggs and larvae of A. ocellatus under stereomicroscopy and scanning electron microscopy. Eggs from natural spawnings were taken to hatcheries, collected at previously established time periods and then analysed. Oscar's eggs are demersal, adhesive and fragile to touch, with a slightly oval shape. The fertile eggs are yellowish in colour and when unfertilized are a white opaque colour. In the initial collection (IC), the majority of eggs were found to be at the gastrula phase with 30% epiboly. At 12 h after the IC, the formation of the embrionary axis and somites was observed, followed by differentiation of the tail and of the head. Fifteen hours after the IC, the emergence of the optic and otic vesicles, and of adhesive glands and the yolk pigmentation was observed. Larval hatching took place between 46 and 58 h after the first collection, at an average temperature of 27.45 ± 2.13°C. The larval stage was characterized by the development of the heart, fins, branchial apparatus, neuromasts, taste buds and adhesive glands on the head. Larval development to yolk absorption took a period of 257 h. These results provide important information for reproduction, rearing and preservation of A. ocellatus.

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Novelties of gasteroid fungi, earthstars and puffballs, from the Brazilian Atlantic rainforest

Anales del Jardín Botánico de Madrid ◽

10.3989/ajbm.2422 ◽

2016 ◽

Vol 73 (2) ◽

pp. 045

Author(s):

Dönis Da Silva Alfredo ◽

Julieth De Oliveira Sousa ◽

Elielson Jacinto de Souza ◽

Luana Mayra Nunes Conrado ◽

Iuri Goulart Baseia

Keyword(s):

Electron Microscopy ◽

Hot Spots ◽

New Record ◽

Morphological Characters ◽

Minas Gerais ◽

Southeastern Part ◽

The World ◽

Brazilian Atlantic Rainforest ◽

Scanning Electron ◽

Forest Biome

The goal of this study was to increase the knowledge of gasteroid fungi in Atlantic Forest biome, which is considered one of the most important hot-spots of the world. Field expeditions were carried out in the Reserva Biologica Municipal de Santa Rita Mitzi Brandao area, in the southeastern part of Minas Gerais. 39 samples belonging to 11 species of earthstars and puffballs are new record for Minas Gerais, Geastrum javanicum Lév., Bovista cunninghamii Kreisel, and Lycoperdon lambinonii Demoulin are first records for Brazil. Information about the locality, morphological characters, illustrations of the basidiomata, scanning electron microscopy (SEM) of the basidiospores, as well as its distribution in Brazil, are given for all species.

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A new species of the diatom genus Campylodiscus (Bacillariophyta) from Dongtan, Chongming Island, China

Phytotaxa ◽

10.11646/phytotaxa.115.2.3 ◽

2013 ◽

Vol 115 (2) ◽

pp. 49

Author(s):

Patrick Kociolek ◽

Bo Wu ◽

Qi Liu ◽

Quanxi Wang

Keyword(s):

Electron Microscopy ◽

Scanning Electron Microscopy ◽

New Species ◽

Small Species ◽

Chongming Island ◽

The World ◽

Diatom Genus ◽

A New Species ◽

Scanning Electron

A new species of genus Campylodiscus is described, Campylodiscus sinensis sp. nov., from Dongtan, Chongming Island, Shanghai, China. It is a small species compared with other species in genus Campylodiscus, with two characteristic kidney-shaped bumps externally and pyramidal verrucae. Light and scanning electron microscopy characteristics of this taxon support its systematic placement in the genus Campylodiscus. This new species is compared with other small species in genus Campylodiscus from around the world.

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New phytoptids (Eriophyoidea, Phytoptidae) from southern African endemic dicotyledons: description of two new species of Solenocristus n. g. and updated key to world genera of the tribe Sierraphytoptini

Systematic and Applied Acarology ◽

10.11158/saa.23.7.7 ◽

2018 ◽

Vol 23 (7) ◽

pp. 1290 ◽

Cited By ~ 1

Author(s):

Philipp Chetverikov ◽

Charnie Craemer ◽

Stefan Neser ◽

Sarah Zukoff

Keyword(s):

Electron Microscopy ◽

South Africa ◽

Scanning Electron Microscopy ◽

New Species ◽

Western Cape ◽

The World ◽

Two New Species ◽

Temperature Scanning ◽

Scanning Electron ◽

Conventional Light Microscopy

A new phytoptid genus and two new species, Solenocristus karooensis n. g. & n. sp. and S. searsius n. sp. (Phytoptidae, Sierraphytoptinae, Sierraphytoptini), were collected in the Eastern and Western Cape provinces of South Africa, from southern African endemic dicotyledonous trees Schotia afra (Fabaceae) and Searsia lucida (Anacardiaceae) respectively. They are described and illustrated with the aid of conventional light microscopy and low temperature scanning electron microscopy. Females of both new species possess a distinct pregenital plate divided into two parts by a subcuticular medial ridge. Additionally, in S. karooensis n. sp. the movable digit of the male chelicerae varies in length: among six observed males, three males had a movable digit (md) twice shorter than the fixed digit (fd), in one male it was slightly shorter than fd and in the remaining two males md and fd were of the same length. An updated key to the world genera of the tribe Sierraphytoptini is given. The key incorporates data on seven sierraphytoptine genera including three genera (Neoprothrix Reis & Navia, Solenoplatilobus Chetverikov & Craemer and Solenocristus n. g.) which were described since the last generic key of Eriophyoidea by Amrine et al. (2003) was published.

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Anisakid larva parasitizing Plagioscion squamosissimus in Marajó Bay and Tapajós River, state of Pará, Brazil

Revista Brasileira de Parasitologia Veterinária ◽

10.1590/s1984-29612016034 ◽

2016 ◽

Vol 25 (4) ◽

pp. 492-496 ◽

Cited By ~ 2

Author(s):

Gabrielle Fontenelle ◽

Marcelo Knoff ◽

Nilza Nunes Felizardo ◽

Eduardo José Lopes Torres ◽

Edilson Rodrigues Matos ◽

...

Keyword(s):

Electron Microscopy ◽

Scanning Electron Microscopy ◽

Optical Microscopy ◽

Infection Intensity ◽

First Record ◽

South American ◽

Taxonomic Identification ◽

Infection Site ◽

Anisakid Nematodes ◽

Scanning Electron

Abstract In November 2014 and May 2015, a total of 44 specimens of the South American silver croaker Plagioscion squamosissimus were collected: 30 in Marajó Bay and 14 in the Tapajós River, state of Pará, Brazil. The aim was to investigate the presence of anisakid nematodes and determine their parasitism indices and sites of infection, because of their importance regarding health inspection. Sixty-nine Anisakis sp. larvae were found; among them, 16 larvae in seven fish collected in Marajó Bay and 53 larvae in four fish in the Tapajós River. The parasitism indices of the nematodes collected from the fish in Marajó Bay comprised prevalence (P) of 23%, mean infection intensity (MI) of 2.28, mean abundance (MA) of 0.53, range of infection (RI) of 1-4, and infection site (IS) in the mesentery. The fish from the Tapajós River showed P = 28%, MI = 13.2, MA = 3.8, RI = 1-22, and IS = mesentery and intestine. To assist in taxonomic identification, images of the specimens obtained through optical microscopy with Nomarski's interferential contrast system and scanning electron microscopy were used. This is the first record of Anisakis sp. parasitizing P. squamosissimus.

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