scholarly journals Common bean breeding for resistance to anthracnose and angular leaf spot assisted by SCAR molecular markers

2012 ◽  
Vol 12 (1) ◽  
pp. 34-42 ◽  
Author(s):  
Gilmar Silvério da Rocha ◽  
Luis Paulo Lelis Pereira ◽  
Pedro Crescêncio Souza Carneiro ◽  
Trazilbo José de Paula Júnior ◽  
José Eustáquio de Souza Carneiro
Keyword(s):  
Molecular Markers ◽  
Common Bean ◽  
Leaf Spot ◽  
Plant Architecture ◽  
Colletotrichum Lindemuthianum ◽  
Angular Leaf Spot ◽  
Scar Markers ◽  
Resistant Line ◽  
Genetic Potential ◽  
Pseudocercospora Griseola

The objective of the present study was to assess the genetic potential of inbred carioca common bean families from five populations derived from crossings involving elite lines and a disease-resistant line (Rudá-R), and to assess the efficiency of SCAR molecular markers in selecting plants resistant to anthracnose and angular leaf spot, at the time of bulk formation. Plant architecture, yield and grain type were assessed. Significant effect among families within population was observed, suggesting wide genetic variability for the characters assessed. Twenty-six superior families were selected. The families contained the greatest number of markers, identified by SCAR molecular markers in the F4 generation. Eighteen of these families were resistant to the races 65 and 453 of Colletotrichum lindemuthianum and five were resistant to the race 63.23 of Pseudocercospora griseola. Thus selection assisted by SCAR markers, in the F4 generation, was an important tool in common bean breeding.

Inheritance and development of molecular markers linked to angular leaf spot resistance genes in the common bean accession G10909

2010 ◽  
Vol 28 (1) ◽  
pp. 57-71 ◽  
Author(s):  
George S. Mahuku ◽  
María Antonia Henríquez ◽  
Carmenza Montoya ◽  
Carlos Jara ◽  
Henry Teran ◽  
...  
Keyword(s):  
Molecular Markers ◽  
Common Bean ◽  
Resistance Genes ◽  
Leaf Spot ◽  
Angular Leaf Spot ◽  
The Common

scholarly journals Characterization of the common bean host and Pseudocercospora griseola, the causative agent of angular leaf spot disease in Tanzania

2016 ◽  
Vol 10 (11) ◽  
pp. 238-245 ◽  
Author(s):  
Amos Chilagane Luseko ◽  
Nchimbi-Msolla Susan ◽  
Mbogo Kusolwa Paul ◽  
Gabriel Porch Timothy ◽  
Miryam Serrato Diaz Luz ◽  
...  
Keyword(s):  
Common Bean ◽  
Causative Agent ◽  
Leaf Spot ◽  
Angular Leaf Spot ◽  
Leaf Spot Disease ◽  
Spot Disease ◽  
The Common ◽  
Pseudocercospora Griseola

scholarly journals Short communication: Occurrence of angular leaf spot caused by Pseudocercospora griseola in Phaseolus vulgaris in Asturias, Spain

2017 ◽  
Vol 15 (3) ◽  
pp. e10SC03 ◽  
Author(s):  
Elena Landeras ◽  
Estefanía Trapiello ◽  
Máximo Braña ◽  
Ana J. González
Keyword(s):  
Common Bean ◽  
Leaf Spot ◽  
Short Communication ◽  
Its Region ◽  
Angular Leaf Spot ◽  
Pathogenicity Test ◽  
Nw Spain ◽  
Tropical Areas ◽  
Necrotic Spots ◽  
Pseudocercospora Griseola

Angular leaf spot (ALS) symptoms were observed in 2015 in common bean fields at four locations in Asturias, NW Spain. This disease is frequent in tropical areas and we have no record of its presence in our region, at least in the last 30 years. However, since its detection its presence in the crops has been increasing. Symptoms were necrotic spots on leaves and reddish-brown to black circular spots on pods, stems, branches and petioles. The damage observed in the mentioned crops was highly variable (between 60% and 100% affected leaves), being most severe in crops where no agrochemical treatment were applied. Three strains were selected and identified based in morphological features as Pseudocercospora griseola. The ITS region was amplified by PCR obtaining a sequence that was identical for the three isolates (Acc. No. LT222499). This sequence showed 99-100% similarity with those deposited in databases corresponding to P. griseola. To fulfill Koch's postulates, a pathogenicity test was carried out in two common bean cultivars (‘Andecha’ and ‘Maruxina’). P. griseola was re-isolated from inoculated plants and not from control plants. In cv. ‘Andecha’, chlorosis was observed in all the inoculated plants, before the appearance of spots. Consequently this is the first confirmed report of this pathogen in our region.

scholarly journals Mycovirus inPseudocercospora griseola, the causal agent of angular leaf spot in common bean

2010 ◽  
Vol 56 (5) ◽  
pp. 359-365 ◽  
Author(s):  
Swiany Silveira Lima ◽  
Ana Karina Rodrigues Abadio ◽  
Elza Fernandes Araújo ◽  
Elliot Watanabe Kitajima ◽  
Aloísio Sartorato ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Common Bean ◽  
Leaf Spot ◽  
Rnase A ◽  
Angular Leaf Spot ◽  
Total Nucleic Acid ◽  
Fungal Isolates ◽  
Transmission Electron ◽  
Fungal Phytopathogens ◽  
Pseudocercospora Griseola

Pseudocercospora griseola (Sacc.) Crous & Braun is a widespread fungal phytopathogen that is responsible for angular leaf spot in the common bean ( Phaseolus vulgaris L.). A number of fungal phytopathogens have been shown to harbour mycoviruses, and this possibility was investigated in populations of Pseudocercospora griseola. The total nucleic acid extracts of 61 fungal isolates were subjected to agarose gel electrophoresis. Small fragments (800–4800 bp) could be identified in 42 of the samples. The presence of dsRNA in isolate Ig838 was confirmed by treatment of total nucleic acid with DNase, RNase A, and nuclease S1. Transmission electron microscopy revealed the presence of viral-like particles 40 nm in diameter in the mycelia of 2 fungal isolates, namely 29-3 and Ig838. The transmission of dsRNA by means of conidia was 100% for isolate 29-3, but there was loss of 1–6 fragments of dsRNA in monosporic colonies of isolate Ig848. Cycloheximide treatment failed to inhibit the mycovirus in isolate 29-3, but proved efficient in the elimination of the 2.2, 2.0, 1.8, 1.2 and 1.0 kb fragments in 2 colonies of isolate Ig848. The occurrence of a mycovirus in Pseudocercospora griseola was demonstrated for the first time in the present study.

scholarly journals Screening Common Bean (Phaseolus vulgaris L.) Germplasm for Resistance against Angular Leaf Spot (Pseudocercospora griseola) Disease under Field Condition

2019 ◽  
Vol 8 (1) ◽  
pp. 30
Author(s):  
Yayis Rezene ◽  
Shiferw Mekonin
Keyword(s):  
Common Bean ◽  
Field Condition ◽  
Leaf Spot ◽  
Genetic Resistance ◽  
Angular Leaf Spot ◽  
Eastern Africa ◽  
Leaf Spot Disease ◽  
Sources Of Resistance ◽  
The Common ◽  
Pseudocercospora Griseola

Angular leaf spot (ALS) caused by the fungus Pseudocercospora griseola is one of the most destructive disease in Latin America and eastern Africa countries. The fungus, P. griseola is highly variable and a diverse sources of resistance genes is required to manage this economically important disease. The use of genetic resistance is the most practical and economic way to manage angular leaf spot of the common bean. Common bean (Phaseolus vulgarise L.) germplasm were screened for resistance against Angular leaf spot (ALS) under field conditions at Wonodogenet and Areka Research farms. Out of 300 common bean accessions evaluated only 14 (4.6%) common bean accessions were resistant to naturally epidemics of angular leaf spot disease under field condition. Therefore, all common bean germplasm that showed resistance reaction can be involved in breeding program for the improvement of the common bean.

Appraisal of common bean farming systems under angular leaf spot disease prone environments of the Southern Highlands of Tanzania

2016 ◽  
Vol 50 (5) ◽  
Author(s):  
Rose Mongi ◽  
Pangirayi Tongoona ◽  
Hussein Shimelis ◽  
Julia Sibiya
Keyword(s):  
Common Bean ◽  
Leaf Spot ◽  
Durable Resistance ◽  
Farming Systems ◽  
Farming System ◽  
Angular Leaf Spot ◽  
Leaf Spot Disease ◽  
Production Constraints ◽  
Pseudocercospora Griseola ◽  
Structured Questionnaire

Angular leaf spot caused by Pseudocercospora griseola is the most destructive disease of common bean (Phaseolus vulgaris L) in the Southern Highlands of Tanzania (SHT). The objectives of this study were to assess the farming system and farmers awareness in the ALS disease prone environments of the SHT. A structured questionnaire was designed to collect information from 238 respondents sampled from Mbeya, Njombe, Iringa and Rukwa regions. Data collected included population demography, production constraints, and indicators of poverty. The results showed that ALS widely occurred in the SHT but farmers were not aware of the disease and sources of inocula. Most of the respondents were poor with average yields of less than 500 kg ha-1. Lack of knowledge on ALS disease and the presence of favorable environmental conditions increased chances of P. griseola proliferation. Therefore, trainings on awareness and intensification of extension services is important. Breeding for durable resistance is an overriding consideration towards increasing bean productivity in the SHT.

RAPD and SCAR Markers Linked to a Gene Conferring Resistance to Angular Leaf Spot in Common Bean

2000 ◽  
Vol 148 (2) ◽  
pp. 117-121 ◽  
Author(s):  
S. Sietsche ◽  
A. Borém ◽  
G. A. Carvalho ◽  
R. C. Rocha ◽  
T. J. Paula ◽  
...  
Keyword(s):  
Common Bean ◽  
Leaf Spot ◽  
Angular Leaf Spot ◽  
Scar Markers

scholarly journals Identification of resistance sources of common bean (Phaseolus vulgaris L.) to angular leaf spot (Pseudocercospora griseola)

2019 ◽  
Vol 72 (2) ◽  
pp. 8785-8791
Author(s):  
Diego Rodríguez ◽  
James Beaver ◽  
Consuelo Estévez de Jensen ◽  
Tim Porch
Keyword(s):  
Puerto Rico ◽  
Phaseolus Vulgaris ◽  
Common Bean ◽  
Resistance Genes ◽  
Leaf Spot ◽  
Angular Leaf Spot ◽  
Gene Pools ◽  
Phaseolus Vulgaris L ◽  
Breeding Programs ◽  
Pseudocercospora Griseola

Common bean (Phaseolus vulgaris L.) is the most important edible legume in the world and is an important source of income for farmers and food for millions of families. Angular Leaf Spot (ALS), caused by the fungus Pseudocercospora griseola (Sacc.) Ferraris, is one of the most important diseases in the production of common bean with the potential to cause significant seed yield losses. An effective and environmentally friendly alternative to control this disease is the use of improved varieties that combine resistance genes of Andean and Mesoamerican origin. In this study, the response of 181 bean genotypes (coming from different breeding programs of several countries including the USA, Puerto Rico, Honduras, Ecuador, Colombia, Tanzania, Malawi, and Angola) to two angular spot isolates (races 61:11 and 63:51, prevalent in Isabela and Juana Díaz, Puerto Rico) were evaluated. Many of these genotypes contained resistant genes to different biotic and to abiotic stress. A total of 16 lines were identified to have resistance to both races. The resistant lines include the Andean breeding lines CAL 143, 277 and the Mesoamerican cultivar ‘Ouro Negro’ that possess the resistance genes Phg-5, Phg-1, Phg-3, respectively. This information can help common bean breeding programs to pyramid genes from the Andean and Mesoamerican gene pools to generate varieties with long-lasting resistance to this disease.

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