scholarly journals In vitro antifungal activity and phytochemical characterization of Eugenia uniflora, Libidibia ferrea and Psidium guajava

2020 ◽  
Vol 56 ◽  
Author(s):  
Magda Rhayanny Assunção Ferreira ◽  
Rosilene Rodrigues Santiago ◽  
Walicyranison Plínio Silva-Rocha ◽  
Luanda Barbara Ferreira Canário de Souza ◽  
Maria Graciela Icher Faria ◽  
...  
Keyword(s):  
Antifungal Activity ◽  
Psidium Guajava ◽  
Eugenia Uniflora ◽  
In Vitro Antifungal Activity

scholarly journals Rhizosphere-enriched microbes as a pool to design synthetic communities for reproducible beneficial outputs

2019 ◽  
Author(s):  
Maria-Dimitra Tsolakidou ◽  
Ioannis A Stringlis ◽  
Natalia Fanega-Sleziak ◽  
Stella Papageorgiou ◽  
Antria Tsalakou ◽  
...  
Keyword(s):  
Antifungal Activity ◽  
Fungal Pathogens ◽  
Growth Parameters ◽  
Tomato Plants ◽  
Beneficial Effects ◽  
Negative Effect ◽  
Tomato Growth ◽  
In Vitro Antifungal Activity

Abstract Composts represent a sustainable way to suppress diseases and improve plant growth. Identification of compost-derived microbial communities enriched in the rhizosphere of plants and characterization of their traits, could facilitate the design of microbial synthetic communities (SynComs) that upon soil inoculation could yield consistent beneficial effects towards plants. Here, we characterized a collection of compost-derived bacteria, previously isolated from tomato rhizosphere, for in vitro antifungal activity against soil-borne fungal pathogens and for their potential to change growth parameters in Arabidopsis. We further assessed root-competitive traits in the dominant rhizospheric genus Bacillus. Certain isolated rhizobacteria displayed antifungal activity against the tested pathogens and affected growth of Arabidopsis, and Bacilli members possessed several enzymatic activities. Subsequently, we designed two SynComs with different composition and tested their effect on Arabidopsis and tomato growth and health. SynCom1, consisting of different bacterial genera, displayed negative effect on Arabidopsis in vitro, but promoted tomato growth in pots. SynCom2, consisting of Bacilli, didn't affect Arabidopsis growth, enhanced tomato growth and suppressed Fusarium wilt symptoms. Overall, we found selection of compost-derived microbes with beneficial properties in the rhizosphere of tomato plants, and observed that application of SynComs on poor substrates can yield reproducible plant phenotypes.

scholarly journals IN VITRO ANTIFUNGAL ACTIVITY OF TIMOL AND CARVACROL ON CANDIDA SPECIES

2019 ◽  
Vol 4 ◽  
Author(s):  
Luciana Thaís Rangel Souza ◽  
Cecília Correia Costa ◽  
Mateus Cardoso Oliveira ◽  
Isabel Celeste Caires Pereira Gusmão
Keyword(s):  
Candida Albicans ◽  
Antifungal Activity ◽  
Inhibitory Concentration ◽  
Candida Krusei ◽  
Minimum Fungicidal Concentration ◽  
Sabouraud Agar ◽  
In Vitro Antifungal Activity

Aim: to evaluate the in vitro action of thymol and carvacrol against the yeasts of Candida albicans ATCC10231 and Candida krusei ATCC34135. Method: A laboratory study was performed to evaluate antifungal activity. The characterization of the Minimal Inhibitory Concentration (MIC) of the thymol essential oil was carried out using the technique where the microdilution is performed, in which a plate containing 96 wells is used. The determination of the Minimum Fungicidal Concentration (MFC) was performed by dripping 10 μL of each of the concentrations evaluated on Sabouraud agar plates. Results: The MIC of thymol and carvacrol for C. albicans was 40 μg/mL and for Candida krusei it did not present antifungal activity. While the MIC of nystatin was 0.03mg for both species with thymol and carvacrol. Conclusion: Thymol presented satisfactory antifungal activity against the pathogens studied, but carvacrol did not present antifungal activity.

In vitro Antifungal Activity of a 29-kDa Glycoprotein Purified from the Galls of Quercus infectoria

2005 ◽  
Vol 40 (1-2) ◽  
pp. 43-54 ◽  
Author(s):  
K. Yamunarani ◽  
R. Jaganathan ◽  
R. Bhaskaran ◽  
P. Govindaraju ◽  
R. Velazhahan
Keyword(s):  
Antifungal Activity ◽  
Quercus Infectoria ◽  
In Vitro Antifungal Activity

Formulation and Evaluation of Carbopol Gel Containing Liposomes of Ketoconazole. (Part-II)

2009 ◽  
Vol 1 (2) ◽  
Author(s):  
Rakesh Patel ◽  
Hardik Patel ◽  
Ashok Baria
Keyword(s):  
Antifungal Activity ◽  
Rat Skin ◽  
Albino Rat ◽  
In Vitro Drug Release ◽  
Carbopol Gel ◽  
Liposomal Formulations ◽  
Release Drug ◽  
In Vitro Permeation ◽  
In Vitro Antifungal Activity

The aim of this work was to prepare and evaluate the topical carbopol gel formulation containing ketoconazole encapsulated liposomes. Ketoconazole loaded liposomes were prepared by thin film hydration technique. The prepared liposomes were incorporated into 1% carbopol gel, and the systems were evaluated for in-vitro drug release, drug retention into skin and in-vitro antifungal activity. The in-vitro permeation of ketoconazole using wistar albino rat skin from liposomal gel was compared with that of plain drug gel and also with plain drug cream containing 2% w/w of ketoconazole. The release of ketoconazole from liposomal gel was much slower than from non liposomal formulations. Gel containing liposomal ketoconazole showed maximum antifungal activity after 30 hours over plain ketoconazole gel and cream formulations.

scholarly journals Orodispersible Film Loaded with Enterococcus faecium CRL183 Presents Anti-Candida albicans Biofilm Activity In Vitro

Pharmaceutics ◽  
2021 ◽  
Vol 13 (7) ◽  
pp. 998
Author(s):  
Virgínia Barreto Lordello ◽  
Andréia Bagliotti Meneguin ◽  
Sarah Raquel de Annunzio ◽  
Maria Pía Taranto ◽  
Marlus Chorilli ◽  
...  
Keyword(s):  
Candida Albicans ◽  
Antifungal Activity ◽  
Enterococcus Faecium ◽  
Potato Starch ◽  
Oral Candidiasis ◽  
Candida Spp ◽  
Sem Images ◽  
Control Film ◽  
In Vitro Antifungal Activity

Background: Probiotic bacteria have been emerging as a trustworthy choice for the prevention and treatment of Candida spp. infections. This study aimed to develop and characterize an orodispersible film (ODF) for delivering the potentially probiotic Enterococcus faecium CRL 183 into the oral cavity, evaluating its in vitro antifungal activity against Candida albicans. Methods and Results: The ODF was composed by carboxymethylcellulose, gelatin, and potato starch, and its physical, chemical, and mechanical properties were studied. The probiotic resistance and viability during processing and storage were evaluated as well as its in vitro antifungal activity against C. albicans. The ODFs were thin, resistant, and flexible, with neutral pH and microbiologically safe. The probiotic resisted the ODF obtaining process, demonstrating high viability (>9 log10 CFU·g−1), up to 90 days of storage at room temperature. The Probiotic Film promoted 68.9% of reduction in fungal early biofilm and 91.2% in its mature biofilm compared to the group stimulated with the control film. Those results were confirmed through SEM images. Conclusion: The probiotic ODF developed is a promising strategy to prevent oral candidiasis, since it permits the local probiotic delivery, which in turn was able to reduce C. albicans biofilm formation.

scholarly journals In Vitro Antifungal Activity of Sanguinarine and Chelerythrine Derivatives against Phytopathogenic Fungi

Molecules ◽  
2012 ◽  
Vol 17 (11) ◽  
pp. 13026-13035 ◽  
Author(s):  
Xin-Juan Yang ◽  
Fang Miao ◽  
Yao Yao ◽  
Fang-Jun Cao ◽  
Rui Yang ◽  
...  
Keyword(s):  
Antifungal Activity ◽  
Phytopathogenic Fungi ◽  
Sanguinarine And Chelerythrine ◽  
In Vitro Antifungal Activity
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