Induction of Interleukin-8 Release by Lung Epithelium with Cystic Fibrosis Epithelial Lining Fluid Is Marginally Affected by Inhibitors of Interleukin-1β

2000 ◽  
Vol 20 (1) ◽  
pp. 64-74 ◽  
Author(s):  
Kristin R. Coulter ◽  
Elizabeth D. Allen ◽  
Judy Hart ◽  
Mark D. Wewers ◽  
Robert G. Castile ◽  
...  
Keyword(s):  
Cystic Fibrosis ◽  
Interleukin 8 ◽  
Interleukin 1Β ◽  
Epithelial Lining ◽  
Lung Epithelium ◽  
Epithelial Lining Fluid

scholarly journals CLINICAL CHARACTERISTICS OF CHILDREN WITH CYSTIC FIBROSIS IN KHARKIV REGION

2017 ◽  
Vol 3 (4) ◽  
pp. 166-169
Author(s):  
Ekateryna Yanovska ◽  
Victoria Klimenko ◽  
Elena Pasichnik
Keyword(s):  
Cystic Fibrosis ◽  
Respiratory Tract ◽  
Ct Scan ◽  
Clinical Characteristics ◽  
Epithelial Lining ◽  
Bacteriological Examination ◽  
Epithelial Lining Fluid ◽  
X Ray ◽  
Chest X Ray ◽  
Microbiological Status

The work is devoted to the study of the clinical and paraclinical peculiarities in children with CF (including respiratory tract microbiocenosis) in Kharkiv region. Also it is about the correlation of microbiological status of CF patients with the disease morbidity.Under the supervision were 30 children with cystic fibrosis. They conducted clinical, paraclinical (bacteriological examination of sputum and epithelial lining fluid, chest X-ray, CT scan of lungs) were performed.Clinical and paraclinic (bacteriological examination of sputum and epithelial lining fluid, chest X-ray, CT scan of lungs) were performed.During observations revealed that the condition severity of CF patients is associated with chronic P. aeruginosa infection, and B. cepacia. And also, that none of patients in Kharkiv region has determined  any of pathognomonic respiratory causative microorganisms – M. Tuberculosis and non-tuberculous micobacteria, H. influenza, Ralstonia picketi, condition of infecting by P. Aeruginosa is not identified, and this is the evidence of insufficient laboratory diagnostics.Key words: cystiс fibrosis, children, microflora В.А. Клименко, К.О. Яновська, О.В. ПасічникКЛІНІЧНА ХАРАКТЕРИСТИКА ДІТЕЙ З МУКОВІСЦИДОЗОМ В ХАРКІВСЬКОМУ РЕГІОНІРобота присвячена вивченню клініко-параклінічних особливостей дітей з МВ (в тому числі - мікробіоценоз респіраторного тракту) в Харківському регіоні. А також визначенню кореляції мікробіологічного статусу хворих МВ з тяжкістю перебігу захворювання.Під наглядом були 30 дітей, хворих на муковісцидоз. Їм проведені клінічні, параклінічні (бактеріологічне дослідження мокротиння і промивних вод бронхів, рентгенограма органів грудної клітки, комп'ютерна томографія легенів) дослідження.В ході спостережень виявлено, що тяжкість стану хворих МВ асоційована з хронічною інфекцією P. aeruginosa і B. cepacia. А також, що в Харківському регіоні з патогномонічних респіраторних збудників МВ у жодного хворого не виявлено M. Tuberculosis і non-tuberculous micobacteria, H. influenza, Ralstonia picketi, не визначається статус інфікування P. aeruginosa, що свідчить про незадовільну лабораторну діагностику.Ключові слова: муковісцидоз, діти, мікрофлора. В.А.  Клименко, Е.А. Яновская, Е.В. ПасичникКЛИНИЧЕСКАЯ ХАРАКТЕРИСТИКА ДЕТЕЙ С МУКОВИСЦИДОЗОМ В ХАРЬКОВСКОМ РЕГИОНЕРабота посвящена изучению клинико-параклинических особенностей детей с МВ (в том числе – микробиоценоз респираторного тракта) в Харьковском регионе. А также определению корреляции микробиологического статуса больных МВ с тяжестью течения заболевания.Под наблюдением были 30 детей, больных муковисцидозом. Им проведены клинические, параклинические (бактериологическое исследование мокроты и промывных вод бронхов, рентгенограмма органов грудной клетки, компьютерная томография легких) исследования.В ходе наблюдений выявлено, что тяжесть состояния больных МВ ассоциирована с хронической инфекцией P. aeruginosa и B. cepacia.  А также, что в Харьковском регионе из патогномоничных респираторных возбудителей МВ ни у одного больного не выявлено M. Tuberculosis и non-tuberculous micobacteria, H. influenza, Ralstonia picketi, не определяется статус инфицирования P. aeruginosa, что свидетельствует о неудовлетворительной лабораторной диагностике.Ключевые слова: муковисцидоз, дети,  микрофлора.

scholarly journals Increased Interleukin-8 in Epithelial Lining Fluid of Collapsed Lungs During One-Lung Ventilation for Thoracotomy

Inflammation ◽  
2012 ◽  
Vol 35 (6) ◽  
pp. 1844-1850 ◽  
Author(s):  
Yoshimichi Komatsu ◽  
Hiroshi Yamamoto ◽  
Kenji Tsushima ◽  
Shino Furuya ◽  
Sumiko Yoshikawa ◽  
...  
Keyword(s):  
Lung Ventilation ◽  
Interleukin 8 ◽  
Epithelial Lining ◽  
One Lung Ventilation ◽  
Epithelial Lining Fluid

Hypochlorous acid alters bronchial epithelial cell membrane properties and prevention by extracellular glutathione

2003 ◽  
Vol 95 (6) ◽  
pp. 2444-2452 ◽  
Author(s):  
Charles J. Venglarik ◽  
Julio Girón-Calle ◽  
Amanda F. Wigley ◽  
Ernst Malle ◽  
Nobuo Watanabe ◽  
...  
Keyword(s):  
Cystic Fibrosis ◽  
Epithelial Cells ◽  
Hypochlorous Acid ◽  
Short Circuit ◽  
Membrane Properties ◽  
Epithelial Lining ◽  
Bronchial Epithelial ◽  
Epithelial Lining Fluid ◽  
Airway Injury ◽  
Apical Side

In chronic inflammatory diseases of the airways, such as cystic fibrosis, hypochlorous acid (HOCl) generated by neutrophils is involved in airway injury. We examined the effects of HOCl on 16HBE14o– bronchial epithelial cells by bolus addition or by generation with glucose oxidase plus myeloperoxidase. HOCl produced both carbonyl formation of a discreet number of proteins and modification of surface targets that were recognized by an antibody raised against HOCl-modified protein. Bolus or enzymatically generated HOCl decreased transepithelial resistance, but surprisingly bolus HOCl also increased short-circuit current. Glutathione in lung epithelial lining fluid is an excellent scavenger of HOCl; however, glutathione content is lower in cystic fibrosis epithelial lining fluid due to deficient glutathione transport to the apical side of bronchial-tracheal epithelial cells (Gao L, Kim KJ, Yankaskas JR, and Forman HJ. Am J Physiol Lung Cell Mol Physiol 277: L113–L118, 1999). We found that alteration of the GSH content of apical fluid above 16HBE14o– cells was protective because all HOCl-induced changes were delayed or eliminated by exogenous glutathione within the physiological range. Extrapolating this to cystic fibrosis suggests that HOCl can alter cell function without destruction but that elevating glutathione could be protective.

Leukotriene B4Markedly Elevated in the Epithelial Lining Fluid of Patients with Cystic Fibrosis

1993 ◽  
Vol 148 (4_pt_1) ◽  
pp. 896-901 ◽  
Author(s):  
Michael W. Konstan ◽  
Ronald W. Walenga ◽  
Kathleen A. Hilliard ◽  
Jay B. Hilliard
Keyword(s):  
Cystic Fibrosis ◽  
Epithelial Lining ◽  
Epithelial Lining Fluid

scholarly journals Pharmacodynamics of Moxifloxacin and Levofloxacin at Simulated Epithelial Lining Fluid Drug Concentrations against Streptococcus pneumoniae

2004 ◽  
Vol 48 (4) ◽  
pp. 1215-1221 ◽  
Author(s):  
Naomi R. Florea ◽  
Pamela R. Tessier ◽  
Cuilian Zhang ◽  
Charles H. Nightingale ◽  
David P. Nicolau
Keyword(s):  
Streptococcus Pneumoniae ◽  
Fluoroquinolone Resistance ◽  
Epithelial Lining ◽  
Time Curve ◽  
Bacterial Killing ◽  
Epithelial Lining Fluid ◽  
Log Reduction ◽  
Development Of Resistance ◽  
Drug Concentrations

ABSTRACT Recent clinical failures associated with levofloxacin treatment for Streptococcus pneumoniae infections and growing evidence of frequent mutations in the isolate population have led to increased concerns regarding fluoroquinolone resistance. Our objective was to characterize the efficacies of levofloxacin and moxifloxacin against various genotypes of S. pneumoniae after simulated bronchopulmonary exposures. An in vitro model was used to simulate a levofloxacin concentration of 500 mg and a moxifloxacin concentration of 400 mg, which were previously determined to be the concentrations in the epithelial lining fluid of older adults receiving once-daily dosing. The effects of the drugs were tested against six S. pneumoniae containing various mutations. Bacterial density and resistance were quantitatively assessed over 48 h. The S. pneumoniae isolate with no mutation displayed a 4-log reduction in CFU after treatment with both agents and did not develop resistance. Isolates containing the parC or parE mutation or both mutations regrew and developed resistance when they were exposed to levofloxacin, despite an unbound area under the concentration-time curve (AUC):MIC ratio of ∼100. When the isolate containing the parC and gyrA mutations was exposed to levofloxacin, there was a half-log reduction in the number of CFU compared to that for the control, but the isolate subsequently regrew. Likewise, levofloxacin did not kill the isolate containing the parC, gyrA, and parE mutations. Moxifloxacin sustained the killing of all bacterial isolates tested without the development of resistance. Levofloxacin did not sustain bacterial killing and did not prevent the emergence of further resistance in mutants with the parC or parE mutation or both mutations, even though an unbound AUC:MIC ratio for exposure well above the breakpoint of 30 to 40 established in the literature for S. pneumoniae was maintained. Moxifloxacin was effective against all isolates tested, despite the presence of isolates with two- and three-step mutations, for which the MICs were increased.

scholarly journals Biopharmaceutical Characterization of Nebulized Antimicrobial Agents in Rats: 5. Oseltamivir Carboxylate

2016 ◽  
Vol 60 (8) ◽  
pp. 5085-5087 ◽  
Author(s):  
Danilo Cesar Galindo Bedor ◽  
Sandrine Marchand ◽  
Isabelle Lamarche ◽  
Julian Laroche ◽  
Davi Pereira de Santana ◽  
...  
Keyword(s):  
Antimicrobial Agents ◽  
Area Under The Curve ◽  
Pulmonary Delivery ◽  
Epithelial Lining ◽  
Oseltamivir Carboxylate ◽  
Epithelial Lining Fluid

ABSTRACTThe aim of this study was to determine the biopharmaceutical characteristics of oseltamivir carboxylate (OC) after pulmonary delivery. After OC bolus and intratracheal nebulization (NEB) in rats, blood was collected and bronchoalveolar lavages (BALs) were performed. Epithelial lining fluid (ELF) concentrations were estimated from BAL fluid. The area under the curve (AUC) ratio for ELF to plasma was 842 times higher after NEB than after intravenous (i.v.) administration, indicating that OC nebulization offers a biopharmaceutical advantage over i.v. administration.

Sign in / Sign up

Export Citation Format

Share Document