Effect of Cold Storage on Larval and Adult Anastrepha ludens (Diptera: Tephritidae) Viability in Commercially Ripe, Artificially Infested Persea americana ‘Hass’

2010 ◽  
Vol 103 (6) ◽  
pp. 2000-2008 ◽  
Author(s):  
M. Aluja ◽  
F. Díaz-Fleischer ◽  
J. Arredondo ◽  
J. Valle-Mora ◽  
J. Rull

Antioxidants ◽  
2020 ◽  
Vol 9 (5) ◽  
pp. 403 ◽  
Author(s):  
Celia Vincent ◽  
Tania Mesa ◽  
Sergi Munne-Bosch

(1) Background: Tocochromanols are a group of fat-soluble compounds including vitamin E (tocopherols and tocotrienols) and plastochromanol-8, and just one avocado can contain up to 20% of the required vitamin E daily intake. (2) Methods: HPLC and LC-MS/MS analyses were performed in avocados of various varieties and origin for the identification and quantification of tocopherols, tocotrienols and plastochromanol-8. After selection of the variety with the highest vitamin E content, we evaluated to what extent short- (4 h) and long-term (10 d) cold storage influences the accumulation of tocochromanols. (3) Results: Analyses revealed that “Bacon” avocados (Persea americana Mill. cv. Bacon) were the richest in vitamin E compared to other avocado varieties (including the highly commercialized Hass variety), and they not only accumulated tocopherols (with 110 µg of α-tocopherol per g dry matter), but also tocotrienols (mostly in the form of γ-tocotrienol, with 3 µg per g dry matter) and plastochromanol-8 (4.5 µg per g dry matter). While short-term cold shock did not negatively influence α-tocopherol contents, it increased those of γ-tocopherol, γ-tocotrienol, and plastochromanol-8 and decreased those of δ-tocotrienol. Furthermore, storage of Bacon avocados for 10 d led to a 20% decrease in the contents of α-tocopherol, whereas the contents of other tocopherols, tocotrienols and plastochromanol-8 were not affected. (4) Conclusions: It is concluded that Bacon avocados (i) are very rich in α-tocopherol, (ii) not only contain tocopherols, but also tocotrienols and plastochromanol-8, and (iii) their nutritional vitamin E value is negatively influenced by long-term cold storage.



Author(s):  
Kerry R. Everett

Abstract The avocado (Persea americana Mill.) is from an ancient plant lineage, the Lauraceae. Although evidence for human consumption dates back 15,000 years, commercialisation has occurred only over the last 150 years. The most commonly traded variety was first the green-skin 'Fuerte' (green as it ripens), and more recently 'Hass', on which skin darkens when ripe. Production has been increasing worldwide, and currently about 64 countries produce avocados. The range of climates is from arid to very high rainfall and from tropical to temperate. The minimum daily temperatures are above 5°C in all avocado-growing regions because of frost sensitivity. Apart from avocado sunblotch viroid (ASBVd), most avocado fruit diseases are caused by fungi. Some fungi cause visible symptoms resulting in unmarketable fruit, and other infections in the orchard are symptomless. These symptomless infections express as rots after harvest during cold storage, transport and ripening. Most post-harvest pathogens infect through both the body of the fruit and the stem-end wound, while a few infect only through the stem-end wound. The geographic distribution of these fungi varies possibly because of differences in environmental requirements and effective quarantine measures during trade. Fungal rots can be reduced by the application of fungicides in the orchard, removing inoculum residing in dead branches and mummified fruit in the canopy, ensuring high-calcium levels in the fruit flesh are maintained, careful post-harvest handling and selling fruit as soon after harvest as possible. Some post-harvest fungicides can be effective.





Author(s):  
Henry H. Eichelberger ◽  
John G. Baust ◽  
Robert G. Van Buskirk

For research in cell differentiation and in vitro toxicology it is essential to provide a natural state of cell structure as a benchmark for interpreting results. Hypothermosol (Cryomedical Sciences, Rockville, MD) has proven useful in insuring the viability of synthetic human epidermis during cold-storage and in maintaining the epidermis’ ability to continue to differentiate following warming.Human epidermal equivalent, EpiDerm (MatTek Corporation, Ashland, MA) consisting of fully differentiated stratified human epidermal cells were grown on a microporous membrane. EpiDerm samples were fixed before and after cold-storage (4°C) for 5 days in Hypothermosol or skin culture media (MatTek Corporation) and allowed to recover for 7 days at 37°C. EpiDerm samples were fixed 1 hour in 2.5% glutaraldehyde in sodium cacodylate buffer (pH 7.2). A secondary fixation with 0.2% ruthenium tetroxide (Polysciences, Inc., Warrington, PA) in sodium cacodylate was carried out for 3 hours at 4°C. Other samples were similarly fixed, but with 1% Osmium tetroxide in place of ruthenium tetroxide. Samples were dehydrated through a graded acetone series, infiltrated with Spurrs resin (Polysciences Inc.) and polymerized at 70°C.







2010 ◽  
Vol 48 (01) ◽  
Author(s):  
Q Liu ◽  
DP Schultze ◽  
H Bruns ◽  
M Zorn ◽  
M Büchler ◽  
...  
Keyword(s):  


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