Endotoxin Contamination of Agaricus blazei Murrill Extract Enhances Murine Immunologic Responses and Inhibits the Growth of Sarcoma 180 Implants In Vivo

Platinum-thymine has been found to be a potent antitumor agent, which is quite soluble in water, and lack nephrotoxicity as the dose-limiting factor. The drug has been shown to interact with DNA and inhibits DNA, RNA and protein synthesis in mammalian cells in vitro. This investigation was undertaken to elucidate the cytotoxic effects of piatinum-thymine on sarcoma-180 cells in vitro ultrastructurally, Sarcoma-180 tumor bearing mice were treated with intraperitoneal injection of platinum-thymine 40mg/kg. A concentration of 60μg/ml dose of platinum-thymine was used in in vitro experiments. Treatments were at varying time intervals of 3, 7 and 21 days for in vivo experiments, and 30, 60 and 120 min., 6, 12, and 24th in vitro. Controls were not treated with platinum-thymine.Electron microscopic analyses of the treated cells in vivo and in vitro showed drastic cytotoxic effect.

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Chemical composition, antitumor activity, and toxicity of essential oil from the leaves of Lippia microphylla

Zeitschrift für Naturforschung C ◽

10.1515/znc-2014-4138 ◽

2015 ◽

Vol 70 (5-6) ◽

pp. 129-137 ◽

Cited By ~ 5

Author(s):

Aline L. Xavier ◽

João Carlos L.R. Pita ◽

Monalisa T. Brito ◽

Déborah R.P. Meireles ◽

Josean F. Tavares ◽

...

Keyword(s):

Chemical Composition ◽

Antitumor Activity ◽

Liver Function ◽

Essential Oil ◽

Pharmacological Study ◽

Hematological Toxicity ◽

Sarcoma 180 ◽

Hemolytic Assay ◽

In Vivo Antitumor Activity

Abstract The chemical composition, antitumor activity and toxicity of the essential oil from Lippia microphylla leaves (OEL) were investigated. The major constituents were thymol (46.5%), carvacrol (31.7%), p-cymene (9%), and γ-terpinene (2.9%). To evaluate the toxicity of OEL in non-tumor cells, the hemolytic assay with Swiss mice erythrocytes was performed. The concentration producing 50% hemolysis (HC50) was 300 μg/mL. Sarcoma 180 tumor growth was inhibited in vivo 38% at 50 mg/kg, and 60% at 100 mg/kg, whereas 5-FU at 50 mg/kg caused 86% inhibition. OEL displays moderate gastrointestinal and hematological toxicity along with causing some alteration in liver function and morphology. However, the changes were considered reversible and negligible in comparison to the effects of several anticancer drugs. In summary, OEL displays in vivo antitumor activity and a moderate toxicity, which suggests further pharmacological study.

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In Vivo and in Vitro Proliferation Kinetics of Sarcoma 180 in Solid Form

Tumori Journal ◽

10.1177/030089167205800504 ◽

1972 ◽

Vol 58 (5) ◽

pp. 335-339 ◽

Cited By ~ 3

Author(s):

Rosella Silvestrini ◽

Ornella Sanfilippo ◽

Luigi Lenaz

Keyword(s):

Double Labelling ◽

Pulse Labelling ◽

Sarcoma 180 ◽

In Vitro Proliferation ◽

Proliferation Kinetics ◽

Human Solid Tumors ◽

A Cell ◽

Kinetics Of

In order to obtain data for setting up a rapid and relatively inexpensive method for studying the proliferation kinetics of human solid tumors, we have determined the kinetic parameters of an experimental solid tumor (Sarcoma 180). The curve of labelled mitosis after pulse labelling with 3H thymidine and the 3H and 14C thymidine double labelling technic on tumor samples incubated in vitro with the labelled precursors were used. A method of digestion of the tissue with hyaluronidase to obtain a cell suspension is described. This method allows easy identification of cells labelled with 3H or 14C thymidine. The two methods yielded reproducible results, the labelling index being 45%, and the duration of S phase 9.9 hours. The in vitro double labelling method with subsequent hyaluronidase digestion is proposed for studying the proliferation kinetics of solid malignancies.

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IN VIVO ANTITUMOR ACTIVITY OF PHYTOCHEMICAL PITC-2 OBTAINED FROM TISSUE CULTURED PLANT PLUCHEA INDICA ON SARCOMA-180 SOLID TUMOR MICE MODEL

Asian Journal of Pharmaceutical and Clinical Research ◽

10.22159/ajpcr.2018.v11i4.23968 ◽

2018 ◽

Vol 11 (4) ◽

pp. 211

Author(s):

Soumita Goswami ◽

Souvik Debnath ◽

Saumen Karan ◽

Tapan Kumar Chatterjee

Keyword(s):

Antitumor Activity ◽

Solid Tumor ◽

Sarcoma 180 ◽

Tumor Cell Proliferation ◽

Mice Model ◽

Ki 67 ◽

Cycle Arrest ◽

In Vivo Antitumor Activity ◽

G1 Cell Cycle Arrest

Objective: PITC-2 was isolated from the methanolic root extract of tissue cultured medicinal plant Pluchea indica (L.) Less. PITC-2 is a thiophene derivative which is 2-(Prop-1-ynyl)-5(5,6-dihydroxyhexa-1,3-diynyl)-thiophene. The main objective of the study is to evaluate the in vivo antitumor activity of PITC 2 against sarcoma-180 cancer cell in Swiss albino mice.Methods: The antitumor activity was evaluated by treatment with PITC-2 at a dose of 2.5 and 5 mg/kg b.w for 21 days on sarcoma-180 mice model. Cell viability was studied using 3-(4, 5- dimethylthiazol -2-yl)-2, 5-diphenyl tetrazolium bromide assay and cell apoptosis, G1 cell cycle arrest and reduction in tumor cell proliferation were evaluated by histopathological analysis and Bcl-2, cyclic-D1, and Ki-67 protein expression through immunohistochemistry study.Results: Precisely, PITC-2 had a cytotoxic effect on various in vitro cancer cells. Significant decreases in solid tumor volume and weight along with increase lifespan also observed. The histopathological and immunohistopathological examination indicates that PITC-2 induces apoptosis, typical morphological changes and suppresses tumor cell proliferation along with G1 cell cycle arrest through the downregulation of the intratumoral expression of Bcl-2, cyclic D1, and Ki-67 and thus highlighting antiproliferative and apoptotic properties against sarcoma-180 in vivo solid tumor model.Conclusion: The present results clearly demonstrate that PITC-2 significantly inhibits sarcoma-180 cell growth in a dose-dependent manner in in vivo mice model. Besides this, the study reveals a comprehensive perception of the possible mechanism behind the antitumor activity of PITC-2 by significant changes in the morphological, hematological, biochemical parameters in sarcoma-180 cells.

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Adaptation of Columbia SK Virus to Sarcoma 180 Ascites Tumor Cells in vivo and Passage in the Cells in vitro.

Experimental Biology and Medicine ◽

10.3181/00379727-108-26904 ◽

1961 ◽

Vol 108 (1) ◽

pp. 244-248

Author(s):

E. Furusawa ◽

W. Cutting

Keyword(s):

Tumor Cells ◽

Sarcoma 180 ◽

Ascites Tumor

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ALTERATION OF HEAVY METAL CONCENTRATION IN IRRADIATED SARCOMA IN VIVO

International Journal of PIXE ◽

10.1142/s012908359200049x ◽

1992 ◽

Vol 02 (04) ◽

pp. 461-467 ◽

Cited By ~ 4

Author(s):

S. HARADA ◽

P. LI ◽

T. YANAGISAWA ◽

K. SERA ◽

S. FUTATSUKAGAWA ◽

...

Keyword(s):

Heavy Metal ◽

Electron Beam ◽

Metal Concentration ◽

Heavy Metal Concentration ◽

Sarcoma 180 ◽

X Ray ◽

Zn And Cu In ◽

Cu And Zn

Alterations of heavy metal concentration in inoculated Sarcoma -180 after irradiation by 6MeV electron beam at the doses of 5Gy were evaluated in vivo in BALB/c mice, by Particle Induced X -ray Emission (PIXE). Analysis was performed for K, Cl, S, Fe, Zn and Cu. In the irradiation of 5Gy, the concentration of K and Cl have revealed the fast incremment at 1 hour after irradiattion and the fast decremment after that time. As for the S, Fe, Cu and Zn, their concentrations have revealed the fast incremment at 1 hour after irradiattion and the slow decremment from 1 hour after irradiation (except for slow increment from 1 hour to 1 day after).

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