Ammonium affects cell viability to inhibit root growth in Arabidopsis

It has been previously reported that POST-applied isoxaben can effectively control established hairy bittercress. Experiments were conducted to determine the relative importance of root vs. foliar entry of POST-applied isoxaben. At a common isoxaben rate of 0.56 kg/ha, foliar-only and foliar plus soil applications provided 10.5 and 23.3% control, respectively, as determined by fresh weight reduction. In contrast, soil-only application provided 47.0% control. Hairy bittercress foliar absorption of14C–isoxaben did not exceed 15% of the amount applied after 72 h. Therefore, the comparatively less effectiveness of foliar-only applications may be attributed primarily to limited absorption. Minimal isoxaben concentration required to inhibit root growth of hydroponically grown hairy bittercress was 0.0025 mg/L. Higher concentrations were required to produce a response in the foliage. Sorption of isoxaben by pine bark rooting substrate, typical of what is used in container nursery production, exceeded 99% of amount applied after 36 h. Even with 99% sorption, the probable concentration within the aqueous phase remains sufficient to inhibit hairy bittercress root growth. Additional studies with14C–isoxaben established that approximately 35% of the root-absorbed isoxaben was translocated into the foliage. Translocation from the roots into the foliage was reduced to 16% when the experiment was repeated during environmental conditions less favorable for vegetative growth (i.e., longer day length and higher temperature). Results indicate that the control of hairy bittercress with POST-applied isoxaben is likely the result of root absorption and root-growth inhibition. Expression of phytotoxicity within the foliage is also a component, but is dependent upon the root-absorbed isoxaben being translocated into the foliage. Extent of this translocation is dependent upon plant maturity and prevalent environmental conditions.

Download Full-text

Bioactive l-DOPA induced quinoprotein formation to inhibit root growth of cucumber seedlings

Journal of Pesticide Science ◽

10.1584/jpestics.d13-005 ◽

2013 ◽

Vol 38 (2) ◽

pp. 68-73 ◽

Cited By ~ 8

Author(s):

Muhammad Naeem Mushtaq ◽

Yukari Sunohara ◽

Hiroshi Matsumoto

Keyword(s):

Root Growth ◽

Cucumber Seedlings ◽

Inhibit Root Growth

Download Full-text

Mild High Concentrations of Boric Acid Repress Leaf Formation and Primary Root Growth in Arabidopsis Seedlings While Showing Anti-apoptotic Effects in a Mutant with Compromised Cell Viability

Journal of Plant Growth Regulation ◽

10.1007/s00344-021-10523-y ◽

2021 ◽

Author(s):

César Emiliano Tapia-Quezada ◽

León Francisco Ruiz-Herrera ◽

Pedro Iván Huerta-Venegas ◽

José López-Bucio

Keyword(s):

Root Growth ◽

Cell Viability ◽

Boric Acid ◽

Primary Root ◽

Leaf Formation ◽

Primary Root Growth ◽

High Concentrations ◽

Apoptotic Effects

Download Full-text

A chemical genetic screen reveals that iminosugar inhibitors of plant glucosylceramide synthase inhibit root growth in Arabidopsis and cereals

Scientific Reports ◽

10.1038/s41598-018-34749-1 ◽

2018 ◽

Vol 8 (1) ◽

Cited By ~ 2

Author(s):

Michael D. Rugen ◽

Mathieu M. J. L. Vernet ◽

Laila Hantouti ◽

Amalia Soenens ◽

Vasilios M. E. Andriotis ◽

...

Keyword(s):

Root Growth ◽

Genetic Screen ◽

Glucosylceramide Synthase ◽

Chemical Genetic ◽

Inhibit Root Growth

Download Full-text

Effects of Aluminum and pH on Root Growth and Cell Viability in Arabidopsis thaliana Strain Landsberg in Hydroponic Culture

Plant and Cell Physiology ◽

10.1093/oxfordjournals.pcp.a078740 ◽

1995 ◽

Cited By ~ 1

Keyword(s):

Arabidopsis Thaliana ◽

Root Growth ◽

Cell Viability ◽

Hydroponic Culture

Download Full-text

Perspectives on Discovery of Microbial Phytotoxins with Herbicidal Activity

Weed Technology ◽

10.1017/s0890037x00032395 ◽

1988 ◽

Vol 2 (4) ◽

pp. 525-532 ◽

Cited By ~ 16

Author(s):

Horace G. Cutler

Keyword(s):

Natural Products ◽

Root Growth ◽

Specific Activity ◽

High Specific Activity ◽

Herbicidal Activity ◽

Biologically Active ◽

Natural State ◽

Natural Herbicides ◽

Synthetic Derivative ◽

Inhibit Root Growth

Biologically active natural products of microbial origin are attractive candidates for possible use in agriculture. They may be obtained by fermentation, used in their natural state, or subjected to synthetic modification for specific uses. These natural products are characterized by high specific activity and high selectivity, and they are biodegradable. The structures are extremely diverse and represent many classes of compounds ranging from very complex to simple. Cyclocarbamide A and B, fromStreptoverticilliumsp., have marked preemergence herbicidal activity. Nigerazine A and B, fromAspergillus nigervan Tieghem, also inhibit root growth in certain plants. Citreoviridin, fromPenicillium charlesiiSmith, preferentially controls the growth of monocotyledonous plants, as does a synthetic derivative of cladosporin, fromAspergillus repensDeBary, which bleaches chloroplasts. The 12-membered fungal macrolides (macrocyclic lactones) also inhibit root growth in many test plants and offer templates for further synthetic work. Herbicidins, fromStreptomyces saganonensis, are particularly effective against barnyardgrass, goosegrass, tufted mannagrass, and green panicum.Alternaria eichorniaeNag Raj et Ponnappa produces a toxin that is active against waterhyacinth and represents one of the more exotic structures. The macrocyclic trichothecenes are a significant class of natural products that tend to concentrate against a gradient in seeds of certain plants, which resist these microbially derived metabolites thereby producing seed with “built-in” natural herbicides.

Download Full-text

A glycoconjugate from corms of saffron plant (Crocus sativus L.) inhibits root growth and affects in vitro cell viability

Journal of Experimental Botany ◽

10.1093/jexbot/51.345.731 ◽

2000 ◽

Vol 51 (345) ◽

pp. 731-737 ◽

Cited By ~ 4

Author(s):

José A. Fernández ◽

Julio Escribano ◽

Abel Piqueras ◽

Joaquín Medina

Keyword(s):

Root Growth ◽

Cell Viability ◽

Crocus Sativus ◽

Crocus Sativus L

Download Full-text

DNA damage inhibits root growth by enhancing cytokinin biosynthesis in Arabidopsis thaliana

10.1101/2020.06.19.160838 ◽

2020 ◽

Author(s):

Naoki Takahashi ◽

Soichi Inagaki ◽

Kohei Nishimura ◽

Hitoshi Sakakibara ◽

Ioanna Antoniadi ◽

...

Keyword(s):

Dna Damage ◽

Root Growth ◽

Plant Root ◽

Root Tip ◽

Dna Double Strand Breaks ◽

Cytokinin Biosynthesis ◽

Strand Breaks ◽

Dna Damage Signaling ◽

Expression Of Genes ◽

Inhibit Root Growth

AbstractPlant root growth is influenced by external factors to adapt to changing environmental conditions. However, the mechanisms by which environmental stresses affect root growth remain elusive. Here we found that DNA double-strand breaks (DSBs) induce the expression of genes for the synthesis of cytokinin hormones and enhance the accumulation of cytokinins in the Arabidopsis root tip. This is a programmed response to DSBs through the DNA damage signaling pathway. Our data showed that activation of cytokinin signalling suppresses the expression of PIN-FORMED genes that encode efflux carriers of another plant hormone, auxin, thereby disturbing downward auxin flow and causing cell cycle retardation in the G2 phase. Elevated cytokinin signalling also promotes an early transition from cell division to endoreplication, resulting in a reduction of the root meristem size. We propose that in response to DNA stress, plants inhibit root growth by orchestrating hormone biosynthesis and signalling.

Download Full-text