Sexual maturation in male mice treated with cyproterone acetate from birth to puberty

1984 ◽  
Vol 102 (1) ◽  
pp. 103-107 ◽  
Author(s):  
Ch. Jean-Faucher ◽  
M. Berger ◽  
M. De Turckheim ◽  
G. Veyssiere ◽  
Cl. Jean
Keyword(s):  
Seminal Vesicle ◽  
Sexual Maturation ◽  
Cyproterone Acetate ◽  
Vas Deferens ◽  
Male Mice ◽  
Preputial Gland ◽  
Early Stages ◽  
Full Development ◽  
Accessory Sex Organs

ABSTRACT Cyproterone acetate was administered every 2 days from 1 to 39 days of age to male mice which were killed 24 h or 20 days after the last injection. Cyproterone acetate caused a significant reduction in the relative weights of the epididymis, vas deferens, seminal vesicle and preputial gland, which was still evident at 60 days after birth. Testicular and epididymal androgens (testosterone and dihydrotestosterone) and circulating LH and FSH concentrations were equal to or higher than those of controls at 60 days. Cyproterone acetate did not inhibit spermatogenesis but all males were infertile. The results suggest that the peripheral effects of testosterone are necessary, during early stages of sexual maturation, in order to obtain subsequent full development of the accessory sex organs. J. Endocr. (1984) 102, 103–107

Permanent changes in the functional development of accessory sex organs and in fertility in male mice after neonatal exposure to cyproterone acetate

1985 ◽  
Vol 104 (1) ◽  
pp. 113-120 ◽  
Author(s):  
Ch. Jean-Faucher ◽  
M. Berger ◽  
M. De Turckheim ◽  
G. Veyssiere ◽  
Cl. Jean
Keyword(s):  
Cyproterone Acetate ◽  
Vas Deferens ◽  
Neonatal Exposure ◽  
Male Mice ◽  
Short Term ◽  
Sexual Organ ◽  
Organ Weights ◽  
Functional Development ◽  
Accessory Sex Organs ◽  
Androgen Levels

ABSTRACT Male mice were injected daily with cyproterone acetate for 10 consecutive days during one of the four following periods: 1–10 days, 11–20 days, 21–30 days or 31–40 days. At all stages studied cyproterone acetate caused a significant reduction in the relative weights of epididymis, vas deferens, preputial gland and seminal vesicle in males killed 24 h after the last injection; the androgen content (testosterone + dihydrotestosterone) of the accessory sex organs was also reduced but the differences were not always significant. Cyproterone acetate treatment from 1 to 10 days resulted in a definitive reduction in the relative weights of all accessory sex organs studied and when injected from 11 to 20 days in epididymis and vas deferens. When cyproterone acetate was injected after 20 days of age, the inhibition of sexual organ weights was reversible and at adulthood organs were normally developed. Cyproterone acetate treatment induced a high percentage of infertile males only when injected from 1 to 10 days. Spermatogenesis, androgen levels in plasma and accessory sex organs, and sexual behaviour were not affected in sterile males. These results suggest that the functional development of accessory sex organs can be permanently affected by short-term neonatal exposure to endogenous androgens. J. Endocr. (1985) 104, 113–120

Testosterone and dihydrotestosterone levels in epididymis, vas deferens, seminal vesicle and preputial gland of mice after hCG injection

1985 ◽  
Vol 23 (2) ◽  
pp. 201-205 ◽  
Author(s):  
Christiane Jean-Faucher ◽  
Michel Berger ◽  
Marc De Turckheim ◽  
Georges Veyssiere ◽  
Claude Jean
Keyword(s):  
Seminal Vesicle ◽  
Vas Deferens ◽  
Preputial Gland

Evidence for reversible and irreversible biochemical defects in accessory sex organs and kidney of neonatally androgenized male mice

1989 ◽  
Vol 121 (1) ◽  
pp. 121-128 ◽  
Author(s):  
G. Veyssiere ◽  
Ch. Jean-Faucher ◽  
M. Berger ◽  
Cl. Jean
Keyword(s):  
Protein Content ◽  
Seminal Vesicle ◽  
Vas Deferens ◽  
Polyacrylamide Gel Electrophoresis ◽  
Seminal Vesicles ◽  
Protein Profiles ◽  
Adult Mice ◽  
Accessory Sex Organs ◽  
Neonatal Administration ◽  
Androgen Levels

Abstract. Studies were conducted to evaluate the effects of neonatal administration of supraphysiological doses of testosterone on the growth, hormone responsiveness, DNA and protein content, and protein profiles of the epididymis, vas deferens and seminal vesicles in adult mice. Results indicate that in androgenized males, testicular growth (DNA and protein content), circulating and organ androgen levels, and fertility were significantly depressed. The weights of the epididymis, vas deferens, seminal vesicle and kidney, but not that of the spleen, were significantly diminished subsequently to a reduction of protein (all organs) and DNA (epididymis, vas deferens) content. The efficacy of testosterone in promoting accessory sex organs and kidney growth, in adult castrated males, was persistently reduced in neonatally androgenized males. When assessed by DNA content, the response of all organs (except the seminal vesicle) was similar to that of controls, but it was significantly reduced from 16 to 43% when measured in terms of protein content. The protein profiles from seminal vesicles and vas deferens analysed by polyacrylamide gel electrophoresis, showed reproducible persistent alterations which could be reversed by adult androgen therapy.

EFFECTS OF CYPROTERONE ACETATE ON AGGRESSIVE BEHAVIOUR AND THE SEMINAL VESICLES OF MALE MICE

1970 ◽  
Vol 46 (4) ◽  
pp. 477-481 ◽  
Author(s):  
D. A. EDWARDS
Keyword(s):  
Seminal Vesicle ◽  
Aggressive Behaviour ◽  
Nervous Tissue ◽  
Cyproterone Acetate ◽  
Testosterone Propionate ◽  
Neural Tissue ◽  
Seminal Vesicles ◽  
Male Mice ◽  
Central Nervous Tissue ◽  
Seminal Vesicle Weight

SUMMARY Cyproterone acetate exerted strong anti-androgenic effects on the seminal vesicles of mice, but failed to affect androgen-dependent aggressive behaviour in mice. Groups of adult castrated male mice were treated with (1) testosterone propionate (TP); (2) TP plus cyproterone acetate; or (3) neither TP nor cyproterone acetate, for 5 weeks and given weekly tests for aggressive behaviour. The seminal vesicles were removed after the last test for aggression. Although treatment of mice with cyproterone acetate in combination with TP almost totally suppressed the stimulatory effect of TP on seminal vesicle weight, aggressive behaviour was not affected by cyproterone acetate. It was concluded that cyproterone acetate probably does not influence central nervous tissue and non-neural tissue in the same manner.

EFFECTS OF TESTOSTERONE AND 5α-DIHYDROTESTOSTERONE ON WEIGHT GAIN AND 3H-THYMIDINE INCORPORATION IN ACCESSORY SEX GLANDS OF CASTRATED MALE RATS

1973 ◽  
Vol 74 (2) ◽  
pp. 379-388 ◽  
Author(s):  
P. Tuohimaa ◽  
A. Oksanen ◽  
M. Niemi
Keyword(s):  
Seminal Vesicle ◽  
Mitotic Activity ◽  
Tritiated Thymidine ◽  
Fold Increase ◽  
Male Rats ◽  
List Type ◽  
Preputial Gland ◽  
Constant Weight ◽  
Accessory Sex Organs ◽  
Coagulating Gland

ABSTRACT Albino male rats were injected subcutaneously (sc) one month after castration with 1 mg of testosterone (T) or dihydrotestosterone (DHT) daily for two weeks. Every twelve hours a pair of rats were injected iv with tritiated thymidine and killed one hour later. The first pair of rats were killed 7 h after the first hormone injection. The accessory sex organs were weighed and processed for radio-autography. The weight response of the accessory sex organs was of the same magnitude after T and DHT. However, DHT was slightly more effective in inducing the growth of the seminal vesicle, the coagulating gland, the preputial gland and the epididymis. The organs reached a constant weight in about 8–10 days after the onset of the hormone treatment. The accessory sex organs could be divided into two groups on the basis of their weight response to DHT and T: 1) Highly responsive tissues (seminal vesicle, coagulating gland and prostate) showed a 5–8 fold increase in weight to 1 mg of DHT or T during stimulation over two weeks. 2) Less responsive tissues (epididymis and preputial gland) can only double their weights during the treatment. By measuring the mitotic response to castration and to the administration of DHT or T the tissues could be categorized into two similar groups: Seminal vesicle, coagulating gland and prostate are highly dependent on androgen supply, since their mitotic activity increases dramatically in 42–66 h after the initiation of hormone administration. This first DNA synthetic peak is followed by irregular series of peaks of decreasing amplitude. In six days the fluctuation in the mitotic activity is stabilized to the level of the uncastrated control animals. In addition, these organs respond slightly more rapidly and more extensively to DHT than to T. The epithelial cells of the preputial gland are less dependent on androgen supply. A significant degree of mitotic activity still persists after castration. The mitotic activity increases 18 h after androgen administration. Fluctuations of mitotic activity are retained during the entire two weeks' period.

Testosterone and dihydrotestosterone levels in the epididymis, vas deferens and preputial gland of mice during sexual maturation

1985 ◽  
Vol 8 (1) ◽  
pp. 44-57 ◽  
Author(s):  
Christiane Jean-Faucher ◽  
Michel Berger ◽  
Marc Turckheim ◽  
Georges Veyssiere ◽  
Claude Jean
Keyword(s):  
Sexual Maturation ◽  
Vas Deferens ◽  
Preputial Gland
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