Effects of different doses of cyproterone acetate (CA) on preputial gland structure and activity in intact male mice

ABSTRACT Cyproterone acetate was administered every 2 days from 1 to 39 days of age to male mice which were killed 24 h or 20 days after the last injection. Cyproterone acetate caused a significant reduction in the relative weights of the epididymis, vas deferens, seminal vesicle and preputial gland, which was still evident at 60 days after birth. Testicular and epididymal androgens (testosterone and dihydrotestosterone) and circulating LH and FSH concentrations were equal to or higher than those of controls at 60 days. Cyproterone acetate did not inhibit spermatogenesis but all males were infertile. The results suggest that the peripheral effects of testosterone are necessary, during early stages of sexual maturation, in order to obtain subsequent full development of the accessory sex organs. J. Endocr. (1984) 102, 103–107

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Membrane estrogen receptor α is essential for estrogen signaling in the male skeleton

Journal of Endocrinology ◽

10.1530/joe-18-0406 ◽

2018 ◽

Vol 239 (3) ◽

pp. 303-312 ◽

Cited By ~ 2

Author(s):

H H Farman ◽

K L Gustafsson ◽

P Henning ◽

L Grahnemo ◽

V Lionikaite ◽

...

Keyword(s):

Estrogen Receptor ◽

Estrogen Receptor Α ◽

Estrogen Signaling ◽

Areal Bone Mineral Density ◽

Male Mice ◽

Mineral Density ◽

Intact Male ◽

Trabecular Thickness ◽

Total Body

The importance of estrogen receptor α (ERα) for the regulation of bone mass in males is well established. ERα mediates estrogenic effects both via nuclear and membrane-initiated ERα (mERα) signaling. The role of mERα signaling for the effects of estrogen on bone in male mice is unknown. To investigate the role of mERα signaling, we have used mice (Nuclear-Only-ER; NOER) with a point mutation (C451A), which results in inhibited trafficking of ERα to the plasma membrane. Gonadal-intact male NOER mice had a significantly decreased total body areal bone mineral density (aBMD) compared to WT littermates at 3, 6 and 9 months of age as measured by dual-energy X-ray absorptiometry (DEXA). High-resolution microcomputed tomography (µCT) analysis of tibia in 3-month-old males demonstrated a decrease in cortical and trabecular thickness in NOER mice compared to WT littermates. As expected, estradiol (E2) treatment of orchidectomized (ORX) WT mice increased total body aBMD, trabecular BV/TV and cortical thickness in tibia compared to placebo treatment. E2 treatment increased these skeletal parameters also in ORX NOER mice. However, the estrogenic responses were significantly decreased in ORX NOER mice compared with ORX WT mice. In conclusion, mERα is essential for normal estrogen signaling in both trabecular and cortical bone in male mice. Increased knowledge of estrogen signaling mechanisms in the regulation of the male skeleton may aid in the development of new treatment options for male osteoporosis.

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Preliminary Study on the Anti-radiation Effect of Jen-Sheng-Yang-Yung-Tang

The American Journal of Chinese Medicine ◽

10.1142/s0192415x93000224 ◽

1993 ◽

Vol 21 (02) ◽

pp. 187-195 ◽

Cited By ~ 7

Author(s):

Hsue-yin Hsu ◽

Yau-hui Ho ◽

Shi-Iong Lian ◽

Chun-ching Lin

Keyword(s):

Bone Marrow ◽

Radiation Effect ◽

Bone Marrow Cells ◽

X Rays ◽

Male Mice ◽

X Ray ◽

Colony Forming Units ◽

Before And After ◽

Preliminary Study ◽

Different Doses

Six to seven week old male mice of ICR strain were exposed to different doses of x-rays to determine if Jen-Sheng-Yang-Yung-Tang could be a modifier in the elimination of radiation damage. Colony forming units of bone marrow cells in the spleen (CFUs) were measured before and after x-ray irradiation with intraperitoneal injection of 10 mg/20 g or 20 mg/20 g body weight of Jen-Sheng-Yang-Yung-Tang, once a day for seven consecutive days. The recovery of CFUs and hemocytes counts by 4 Gy irradiation with Jen-Sheng-Yang-Yung-Tang administration was faster for a concentration of 20 mg/20 g than 10 mg/20 g. The measurement of 10-day CFUs showed an increase of radiotolerance in the treatment of 20 mg/20 g administration before x-ray irradiation. The injection of Jen-Sheng-Yang-Yung-Tang accelerated the recovery of hemocyte counts in mice irradiated with 4 Gy x-ray; the effect was especially profound for leukocytes with 20 mg/20 g Jen-Sheng-Yang-Yung-Tang administration after irradiation.

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Neural Determinants of Pulsatile Luteinizing Hormone Secretion in Male Mice

Endocrinology ◽

10.1210/endocr/bqz045 ◽

2020 ◽

Vol 161 (2) ◽

Cited By ~ 4

Author(s):

Su Young Han ◽

Isaiah Cheong ◽

Tim McLennan ◽

Allan E Herbison

Keyword(s):

Luteinizing Hormone ◽

High Frequency ◽

Pulse Generator ◽

Hormone Secretion ◽

Pulse Frequency ◽

Male Mice ◽

Intact Male ◽

Luteinizing Hormone Secretion ◽

Pulse Profile ◽

Lh Secretion

Abstract The gonadotrophin-releasing hormone (GnRH) pulse generator drives pulsatile luteinizing hormone (LH) secretion essential for fertility. However, the constraints within which the pulse generator operates to drive efficient LH pulsatility remain unclear. We used optogenetic activation of the arcuate nucleus kisspeptin neurons, recently identified as the GnRH pulse generator, to assess the efficiency of different pulse generator frequencies in driving pulsatile LH secretion in intact freely behaving male mice. Activating the pulse generator at 45-minute intervals generated LH pulses similar to those observed in intact male mice while 9-minute interval stimulation generated LH profiles indistinguishable from gonadectomized (GDX) male mice. However, more frequent activation of the pulse generator resulted in disordered LH secretion. Optogenetic experiments directly activating the distal projections of the GnRH neuron gave the exact same results, indicating the pituitary to be the locus of the high frequency decoding. To evaluate the state-dependent behavior of the pulse generator, the effects of high-frequency activation of the arcuate kisspeptin neurons were compared in GDX and intact mice. The same stimulus resulted in an overall inhibition of LH release in GDX mice but stimulation in intact males. These studies demonstrate that the GnRH pulse generator is the primary determinant of LH pulse profile and that a nonlinear relationship exists between pulse generator frequency and LH pulse frequency. This may underlie the ability of stimulatory inputs to the pulse generator to have opposite effects on LH secretion in intact and GDX animals.

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Urine from Sexually Mature Intact Male Mice Contributes to Increased Cardiovascular Responses during Free-Roaming and Restrained Conditions

ISRN Veterinary Science ◽

10.5402/2012/185461 ◽

2012 ◽

Vol 2012 ◽

pp. 1-7 ◽

Cited By ~ 2

Author(s):

Dexter L. Lee ◽

Justin L. Wilson

Keyword(s):

Heart Rate ◽

Mean Arterial Pressure ◽

Male Mouse ◽

Cardiovascular Responses ◽

Mature Male ◽

Physical Contact ◽

Male Mice ◽

Intact Male ◽

Baseline Values ◽

Sexually Mature

Pheromones in the urine regulate aggression of male mice and castrated males produce less of these pheromones. We tested the hypothesis that pheromones in the urine of sexually mature-intact (SMI) males placed in the cage bedding of an individually housed male mouse or in a mouse restrainer would contribute to a significant increase in mean arterial pressure (MAP), heart rate (HR), and activity. Sexually mature male C57BL/6 mice were implanted with a biotelemetry transmitter to measure MAP, HR, and activity. Urine (200 μL) from SMI mice placed in the cages of singularly housed male mice caused significant changes above baseline values for MAP (21±4 mmHg), HR (145±25 bpm), and activity (9±2 counts) when compared to urine from castrated mice-induced MAP (11±3 mmHg), HR (70±15 bpm), and activity (5±1 counts). Pretreatment with terazosin significantly reduced the change in MAP (9±3 mmHg), heart rate (90±15 bpm), and activity (4±2 counts) responses to urine from SMI males. Saline did not significantly increase MAP, HR, or activity in any group. During restraint, urine from SMI mice caused a significant change in MAP (5±0.4 mmHg) and HR (17±1 bpm); urine from castrated mice did not cause a significant increase in MAP and HR. Our results demonstrate that a significant increase in MAP, HR, and activity occurs when male mice are exposed to urine pheromones from SMI males. In summary, pheromones in the urine of SMI male excreted in the cage bedding and mouse restrainers contribute to a significant increase in cardiovascular responses in the absence of direct physical contact with a different male mouse or animal handler.

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Synaptonemal complex studies in male mice treated with cyproterone acetate or with testosterone

Mutation Research Letters ◽

10.1016/0165-7992(93)90094-c ◽

1993 ◽

Vol 303 (2) ◽

pp. 45-53 ◽

Cited By ~ 4

Author(s):

Mungo B. Masumbuko ◽

Roger De Meyer ◽

Maria M. Freund

Keyword(s):

Synaptonemal Complex ◽

Cyproterone Acetate ◽

Male Mice

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305 OPTIMIZING DONOR AND RECIPIENT FACTORS IN XENOGRAFTING OF TESTIS TISSUE

Reproduction Fertility and Development ◽

10.1071/rdv22n1ab305 ◽

2010 ◽

Vol 22 (1) ◽

pp. 308

Author(s):

S. Abbasi ◽

A. Honaramooz

Keyword(s):

Recipient Mouse ◽

Male Mice ◽

Intact Female ◽

Intact Male ◽

Immunodeficient Mice ◽

Female Nude ◽

Graft Weight ◽

And Gender ◽

Gonadal Status ◽

Testis Tissue

Grafting of donor mammalian testis tissue into recipient mice allows completion of spermatogenesis in the grafted tissue and therefore can serve as a new option for preservation of male germ line. For testis tissue xenografting, castrated male nude mice typically serve as recipients, each receiving 8 testis tissue fragments; however, no study has comprehensively investigated donor and recipient factors. The objective of this study was to determine the effects of strain of immunodeficient recipient mouse (nude v. SCID), gonadal status (intact v. gonadectomized), and gender (male v. female) on the outcome of testis tissue xenografting. A secondary objective was to determine the optimal number of testis tissue fragments per mouse most suitable for xenografting. Testis parenchyma from newborn piglets were cut into small fragments (5 mg each) and grafted under the back skin of different groups of immunodeficient mice. In Experiment 1, 8 groups of mice (n = 7/group) served as recipients: castrated male nude, intact male nude, ovariectomized female nude, intact female nude, castrated male SCID, intact male SCID, ovariectomized female SCID, and intact female SCID. In Experiment 2, 4 groups of mice (n = 10/group) served as recipients of 2, 4, 8, or 16 testis tissue fragments per mouse. Recipient mice were sacrificed 8 months after grafting and the weight of the grafts and vesicular glands (male mice) were compared among groups by analysis of variance. In Experiment 1, mouse gonadal status (intact v. gonadectomized) did not affect the total graft weight (P > 0.05), but both the recipient mouse strain (nude v. SCID) and gender (male v. female) affected the total graft weight (2460 ± 320.9, 1420 ± 290.0, 758 ± 156.7, and 2780 ± 297.4, mean ± SEM, P < 0.0001 for SCID, nude, female, and male mice, respectively). In Experiment 2, the total graft weight was highest in the group of mice receiving 8 testis tissue fragments (192 ± 76.1, 695 ± 96.5, 2443 ± 338.8, and 1458 ± 305.4, mean ± SEM, P < 0.0001 for 2, 4, 8, or 16 fragment groups, respectively). These results collectively indicate that male SCID mice receiving 8 testis tissue fragments provide optimized conditions for the recovery of largest grafts. Research was supported by grants from the Natural Sciences and Engineering Research Council of Canada (NSERC) and the Saskatchewan Health Research Foundation (SHRF) to A. Honaramooz and scholarships from the Western College of Veterinary Medicine and the International Peace Scholarship to S. Abbasi.

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2-Methoxyestradiol Reduces Angiotensin II–Induced Hypertension and Renal Dysfunction in Ovariectomized Female and Intact Male Mice

Hypertension ◽

10.1161/hypertensionaha.117.09175 ◽

2017 ◽

Vol 69 (6) ◽

pp. 1104-1112 ◽

Cited By ~ 13

Author(s):

Ajeeth K. Pingili ◽

Karen N. Davidge ◽

Shyamala Thirunavukkarasu ◽

Nayaab S. Khan ◽

Akemi Katsurada ◽

...

Keyword(s):

Angiotensin Ii ◽

Renal Dysfunction ◽

Male Mice ◽

Intact Male ◽

Induced Hypertension

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The Antioxidant Effects of Germinating Tartary Buckwheat Vinegar

Applied Mechanics and Materials ◽

10.4028/www.scientific.net/amm.651-653.305 ◽

2014 ◽

Vol 651-653 ◽

pp. 305-312 ◽

Cited By ~ 1

Author(s):

Yun Long Li ◽

Ting Jun Ma ◽

Hong Mei Li ◽

Jun Jun Hu ◽

Jun Sheng Bian ◽

...

Keyword(s):

Antioxidant Capacity ◽

Total Antioxidant Capacity ◽

Dose Group ◽

Low Dose ◽

Positive Control ◽

Control Model ◽

Male Mice ◽

Total Antioxidant ◽

Antioxidant Effects ◽

Different Doses

The present study was to evaluated the antioxidant capacity and the effect to the D - galactose senile mice of buckwheat vinegar which maked by sprouting buckwheat, divided healthy Kunming male mice into blank, positive control, model and three dose groups of vinega made by stirring buckwheat randomly. After 6 weeks, it was determined the activity of antioxidant enzyme and malondialdehyde in mice with different doses groups. The results showed that: the stirring buckwheat vinegar exhibited strong 1,1-diphenyl-2-picrylhydrazyl (DPPH) (IC50 was 0.58*10-2 ) and total antioxidant capacity (210.58 mmolFe2SO4•ml-1 ); the vinegar can droped the level of MDA and improved the activity of SOD in liver of D - galactose senile mice. The low-dose group can improved the activity of SOD in serum. However, for the aging mice, the buckwheat vinegar had no effect on behavior when they were in the new environment, the ability of reduced the activity of TChE in brain and the level of MDA in serum was not significant, at the same time, the activity of GSH-px in the serum couldn't be enhanced.

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Influence of gonadal hormones on the behavioral effects of intermittent hypoxia in mice

AJP Regulatory Integrative and Comparative Physiology ◽

10.1152/ajpregu.00379.2014 ◽

2015 ◽

Vol 308 (6) ◽

pp. R489-R499 ◽

Cited By ~ 3

Author(s):

Taryn G. Aubrecht ◽

Richelle Jenkins ◽

Ulysses J. Magalang ◽

Randy J. Nelson

Keyword(s):

Learning And Memory ◽

Spatial Learning ◽

Intermittent Hypoxia ◽

Upper Airway ◽

Premenopausal Women ◽

Gonadal Hormones ◽

Spatial Learning And Memory ◽

Male Mice ◽

Intact Male ◽

Obstructive Sleep

Obstructive sleep apnea (OSA) is characterized by repetitive upper airway obstruction resulting in cyclic intermittent hypoxia (IH) during sleep in affected individuals. OSA occurs more frequently in postmenopausal than premenopausal women and the severity of OSA increases after menopause. Gonadal hormones can influence brain and behavior; testosterone and estrogens in particular can enhance spatial learning and memory. We hypothesized that estrogens may protect mice from IH-induced hippocampal morphological and behavioral changes. To test this hypothesis we exposed intact or gonadectomized male and female mice to room air or IH [15 cycles/h, 8 h/day, fraction of inspired oxygen (FiO2) nadir of 5%] for a total of 30 days. During the final 4 days of IH, mice were tested for anxiety- and depressive-like behaviors. After cessation of IH exposure mice were tested on the Barnes maze and passive avoidance tests to assess learning and memory. Ovariectomy paired with IH treatment, impaired spatial learning and memory compared to all other female groups. Intact male mice receiving IH treatment also had impaired learning and memory compared with intact or castrated male mice exposed to room air. Learning and memory changes were mirrored by changes in basilar dendritic length of the CA1 region of the hippocampus. These data suggest that estrogens provide protection against IH-induced deficits, whereas androgens partially exacerbate IH-induced deficits on learning and memory.

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