Localization of α-interferon in the human feto-placental unit

1988 ◽  
Vol 119 (3) ◽  
pp. 531-NP ◽  
Author(s):  
A. G. Howatson ◽  
M. Farquharson ◽  
A. Meager ◽  
A. M. McNicol ◽  
A. K. Foulis
Keyword(s):  
Biological Effects ◽  
Placental Tissue ◽  
Paraffin Wax ◽  
Immunocytochemical Study ◽  
Chorionic Villi ◽  
Tissue Sections ◽  
Complex Series ◽  
Human Placental Tissue

ABSTRACT The distribution of α-interferon in human placental tissue was investigated by immunocytochemical study of paraffin wax-embedded tissue sections using a sheep α-interferon antiserum. Fifty-eight placentas of gestational ages from 8 to 40 weeks were examined. α-Interferon was present in the syncytiotrophoblast of the chorionic villi of all placentas and was also in macrophages in 28 cases. The appearances suggest production of interferon in human placental trophoblast and, in view of its diverse biological effects, support the concept of a role for α-interferon in the complex series of events required for successful gestation. J. Endocr. (1988) 119, 531–534

scholarly journals Visualizing ultrastructural details of placental tissue with super-resolution structured illumination microscopy

2020 ◽  
Author(s):  
Luis E. Villegas-Hernández ◽  
Mona Nystad ◽  
Florian Ströhl ◽  
Purusotam Basnet ◽  
Ganesh Acharya ◽  
...  
Keyword(s):  
Cell Biology ◽  
Resolution Enhancement ◽  
Super Resolution ◽  
Placental Tissue ◽  
Structured Illumination ◽  
Structured Illumination Microscopy ◽  
Chorionic Villi ◽  
Tissue Sections ◽  
Formalin Fixed Paraffin ◽  
Formalin Fixed Paraffin Embedded

AbstractSuper-resolution fluorescence microscopy is a widely employed technique in cell biology research, yet remains relatively unexplored in the field of histo-pathology. Here, we describe the sample preparation steps and acquisition parameters necessary to obtain fluorescent multicolor super-resolution structured illumination microscopy (SIM) images of both formalin-fixed paraffin-embedded and cryo-preserved placental tissue sections. We compare super-resolved images of chorionic villi against diffraction-limited deconvolution microscopy and demonstrate the significant contrast and resolution enhancement attainable with SIM. We show that SIM resolves ultrastructural details such as the syncytiotrophoblast’s microvilli brush border, which up until now has been only resolvable by electron microscopy.

Abstract 549: Angiotensin II Attenuates Apelin-36 Release from Human Placental Chorionic Villi

Hypertension ◽  
2013 ◽  
Vol 62 (suppl_1) ◽  
Author(s):  
Liliya M Yamaleyeva ◽  
K. Bridget Brosnihan ◽  
Lauren Anton ◽  
Carolynne McGee ◽  
Heather L Mertz ◽  
...  
Keyword(s):  
Angiotensin Ii ◽  
Placental Tissue ◽  
Inhibitory Effect ◽  
Negative Influence ◽  
Ang Ii ◽  
Tissue Samples ◽  
Chorionic Villi ◽  
Receptor Localization ◽  
Apelin Receptor ◽  
Human Placental Tissue

Angiotensin II (Ang II) and apelin have opposing actions in the regulation of blood pressure, vascular tone and angiogenesis, factors known to be important in pregnancy. Chorionic villi (CV) are essential for the transport of oxygen and metabolites in the maternal-fetal interface. Previous studies from our group demonstrated the upregulation of Ang II in the CV of preeclamptic women. Since the interaction between Ang II and apelin have been suggested in the cardiovascular system, we determined whether Ang II alters the release of apelin from the placental CV obtained from normotensive pregnant (NP) and preeclamptic (PRE) women. We also established whether Ang-(1-7) influences apelin release from the same set of tissue samples. CV were dissected from the placental tissue, washed and incubated for 0, 2 and 16 hours in DMEM/F12 media with or without Ang II (1 nM and 1 μM) or Ang-(1-7) (1 nM and 1 μM) under controlled O2 and CO2 tensions. The unstimulated release of apelin measured by a competitive radioimmunoassay was significantly reduced in the conditioned media from PRE compared to NP chorionic villi at 2 hrs (PRE: 181.1 ± 31 vs. NP: 309.5 ± 51.4 pg/ml, p<0.05). There was a significant increase in apelin release from both NP (267.8 ± 34.8 vs. baseline: 118.4 ± 27.3 pg/ml, p<0.01) and PRE (293.5 ± 25.8 vs. baseline: 122.6 ± 24.8 pg/ml, p<0.01) CV at 16 hours. Ang II or Ang-(1-7) inhibited the release of apelin from CV of the NP (p<0.05) but not of PRE at 2 hours. At 16 hours, however, Ang II or Ang-(1-7) inhibited the release of apelin from both NP and PRE villi (p<0.05). Immunostaining revealed the predominant localization of apelin receptor to syncytiotrophoblasts and fetal vascular cells of CV - areas associated with AT1 receptor localization. Our data showing a negative influence of Ang II on apelin release from chorionic villi suggest an opposing interaction between these peptides in the human placental tissue. Reduced apelin activity would remove its counter-regulatory actions on Ang II-induced endothelial dysfunction, increased vascular resistance, and inflammation - abnormalities which precede and sustain preeclampsia. A surprising inhibitory effect of Ang-(1-7) on apelin suggests a complex regulation of apelin within the RAS.

A Preliminary Study on Human Placental Tissue Impaired by Gestational Diabetes: A Comparison of Gel-Based versus Gel-Free Proteomics Approaches

2016 ◽  
Vol 22 (2) ◽  
pp. 71-82 ◽  
Author(s):  
Marco Roverso ◽  
Maura Brioschi ◽  
Cristina Banfi ◽  
Silvia Visentin ◽  
Silvia Burlina ◽  
...  
Keyword(s):  
Gestational Diabetes ◽  
Placental Tissue ◽  
Human Placental Tissue ◽  
Preliminary Study

Effect of glucose and oxygen deprivation on heme oxygenase expression in human chorionic villi explants and immortalized trophoblast cells

2003 ◽  
Vol 285 (6) ◽  
pp. R1453-R1460 ◽  
Author(s):  
S. D. Appleton ◽  
G. E. Lash ◽  
G. S. Marks ◽  
K. Nakatsu ◽  
J. F. Brien ◽  
...  
Keyword(s):  
Enzymatic Activity ◽  
Protein Content ◽  
Heme Oxygenase ◽  
Glucose Concentration ◽  
Placental Tissue ◽  
Glucose Deprivation ◽  
First Trimester ◽  
Trophoblast Cells ◽  
Chorionic Villi ◽  
Low Glucose

Although hypoxia induces heme oxygenase (HO)-1 mRNA and protein expression in many cell types, recent studies in our laboratory using human placental tissue have shown that a preexposure to hypoxia does not affect subsequent HO enzymatic activity for optimized assay conditions (20% O2; 0.5 mM NADPH; 25 μM methemalbumin) or HO-1 protein content. One of the consequences of impaired blood flow is glucose deprivation, which has been shown to be an inducer of HO-1 expression in HepG2 hepatoma cells. The objective of the present study was to test the effects of a 24-h preexposure to glucose-deprived medium, in 0.5 or 20% O2, on HO protein content and enzymatic activity in isolated chorionic villi and immortalized HTR-8/SVneo first-trimester trophoblast cells. HO protein content was determined by Western blot analysis, and microsomal HO enzymatic activity was measured by assessment of the rate of CO formation. HO enzymatic activity was increased ( P < 0.05) in both placental models after 24-h preexposure to glucose-deficient medium in 0.5 or 20% O2. Preexposure (24 h) in a combination of low O2 and low glucose concentrations decreased the protein content of the HO-1 isoform by 59.6% ( P < 0.05), whereas preexposure (24 h) to low glucose concentration alone increased HO-2 content by 28.2% in chorionic villi explants ( P < 0.05). In this preparation, HO enzymatic activity correlated with HO-2 protein content ( r = 0.825). However, there was no correlation between HO-2 protein content and HO enzymatic activity in HTR-8/SVneo trophoblast cells preexposed to 0.5% O2 and low glucose concentration for 24 h. These findings indicate that the regulation of HO expression in the human placenta is a complex process that depends, at least in part, on local glucose and oxygen concentrations.

scholarly journals Features of the histological structure of the placenta in women with recurrent miscarriage depending on polymorphic variants of the metalloproteinase and hemostasis systems

2020 ◽  
Vol 17 (2) ◽  
pp. 158-169
Author(s):  
T. N. Grinevich ◽  
S. A. Lyalikov ◽  
V. A. Basinsky ◽  
T. T. Shtabinskaya ◽  
C. M. Butolina ◽  
...  
Keyword(s):  
Comparison Group ◽  
Recurrent Miscarriage ◽  
Morphological Characteristics ◽  
Reproductive Function ◽  
Placental Tissue ◽  
Histological Structure ◽  
Chorionic Villi ◽  
Intervillous Space ◽  
Reproductive Losses ◽  
Polymorphic Variants

The article is devoted to the analysis of the morphological characteristics of placental tissue in women with early reproductive losses with recurrent miscarriage, depending on the presence of polymorphic variants of metalloproteinases and hemostasis. It was established that in women with recurrent miscarriage, the area of the trophoblast of the placenta is significantly larger (р < 0.003), the area of the chorionic villi is smaller (р < 0.04) than in the group of women with implemented reproductive function. Thrombosis of the chorionic vessels, necrosis and calcinates in the placenta with PNF were detected in 80.0 % (р = 0.001), 93.3 % (р = 0.001), and 30.0 % (р = 0.049) of cases, respectively, which is significantly higher than in the comparison group. In patients with PNP with the T/T genotype of the 735 C/T polymorphism of the MMP-2 gene, the trophoblast area is significant (р < 0.05) more than with the C/C genotype, acute full-back (р = 0.02) and intervillous hemorrhage (р = 0.02). In women with the C/T genotype of the polymorphism 735 C/T of the MMP-2 gene, edema and dystrophic changes in the chorionic villi, placental necrosis are determined more often (р = 0.02) than with the C/C genotype. In carriers of the T allele of the polymorphism 735 C/T of the MMP-2 gene in the placenta, necrosis is significantly more common than in the CC genotype; the carriage of the T allele is also associated with large values of the relative area of the trophoblast and the stromal-intervillous relation, as well as with a smaller value of the ratio of the intervillous space area to the total area of the photograph.

scholarly journals Seasonally Variant Gene Expression in Full-Term Human Placenta

2020 ◽  
Author(s):  
Danielle A. Clarkson-Townsend ◽  
Elizabeth Kennedy ◽  
Todd M. Everson ◽  
Maya A. Deyssenroth ◽  
Amber A. Burt ◽  
...  
Keyword(s):  
Health Outcomes ◽  
Placental Tissue ◽  
Later Life ◽  
Full Term ◽  
Season Of Birth ◽  
Rhythmic Expression ◽  
Health And Development ◽  
Human Placental Tissue ◽  
Variant Genes ◽  
Ribosomal Activity

ABSTRACTSeasonal exposures influence human health and development. The placenta, as a mediator of the maternal and fetal systems and a regulator of development, is an ideal tissue to understand the biological pathways underlying relationships between season of birth and later life health outcomes. Here, we conducted a transcriptome-wide association study of season of birth in full-term human placental tissue to evaluate whether the placenta may be influenced by seasonal cues. Of the analyzed transcripts, 583 displayed differential expression between summer and winter births (FDR q<0.05); among these, BHLHE40, MIR210HG, and HILPDA had increased expression among winter births (Bonferroni p<0.05). Enrichment analyses of the seasonally variant genes between summer and winter births indicated over-representation of transcription factors HIF1A, VDR, and CLOCK, among others, and of GO term pathways related to ribosomal activity and infection. Additionally, a cosinor analysis found rhythmic expression for approximately 11.9% of all 17,664 analyzed placental transcripts. These results suggest that the placenta responds to seasonal cues and add to the growing body of evidence that the placenta acts as a peripheral clock, which may provide a molecular explanation for the extensive associations between season of birth and health outcomes.

The specific 3H-ouabain binding to Na+/K+-ATPase of the human placental tissue is inhibited by newborn plasma extracts

Steroids ◽  
1988 ◽  
Vol 52 (4) ◽  
pp. 363-364
Author(s):  
Anna Paci ◽  
Silvana Balzan ◽  
Franca Cocci ◽  
Hans Peter Niedermeyer ◽  
Aldo Clerico
Keyword(s):  
Placental Tissue ◽  
Ouabain Binding ◽  
Human Placental Tissue
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