Genetic Effects of Ultra-High-Diluted-Biomedicines Gall 30C, Gall 200C, and Gall 1000C may be Vaccines against Plant and COVID-19 Diseases: Improved Agriculture-Health-Medical-Pharmaceutical-ScienceTechnology-Communication-Issues!

2021 ◽  
Vol 7 (2) ◽  
Author(s):  
Datta SC
Keyword(s):  
Systemic Acquired Resistance ◽  
Acquired Resistance ◽  
Foliar Spray ◽  
Fruit Production ◽  
Genetic Effects ◽  
Human Populations ◽  
Vegetable Crops ◽  
Resistance Response ◽  
Pharmaceutical Science ◽  
Science Technology

The current coronavirus 2 (SARS-CoV-2) outbreaks of Coronavirus disease 2019 (COVID-19) pandemic have emphasized the vulnerability of human populations to novel viral pressures, causing an emergent global pandemic and badly impacts on agriculture-environment-health-socio-economy medical-pharmaceutical-science-technology-communication-issues. And India emphasis on okra, Abelmoschus esculentus (L.) Moench Cv. Ankur-40, the ‘Nature’s-Gift to Human-Disease-Free-Healthy-Life’ and the most ‘Economically-Important Number-One Consumption-Vegetable-Crops,’ is damaged by different pathogens like nematodes, causing the root-knot (RK) disease which is easily controlled by different chemical-pesticides, and so it is an urgent need to develop potential epidemiological and biomedical vaccines. The Ultra-High-Diluted Biomedicines (UHDBM) Gall 30C, Gall 200C, and Gall 100C, prepared from Okra Root-Galls (ORG) or Gall MT, applied by foliar spray @ 20 mL/plant each group respectively, are highly effective against the root-knot disease of okra, with increasing fresh-plant growth and fruit production. The UHDBM; Gall 30C, Gall 200C, and Gall 1000-pretreatments are more effective than the untreated ones, and Gall 200C shows the most potential result in all respects. The genetic-effects of UHDBM thought to induce systemic acquired resistance response of the treated plants through the expression of pathogenesis-related (PR)- proteins-genes (22 to 4 numbers), which are more or less similar molecular range (295 kD to 11 kD) of many coronaviruses, and it will responsible for preventing RK and COVID-19 like variant-virus diseases by inducing defense-resistance or increasing innate-immunity, and enriching agricultureenvironment-health-socio-economy-medical-pharmaceutical-science-technology-communication-issues (AEHSEMPSTCI) with the toxic-free world, and it may help to develop best potential new preventive treatments methods or drug or vaccines, in the field of ‘21st Century COVID-19 like Pandemic in the new normal situation in future.

scholarly journals Economic okra plant act as a preventive-COVID-19 vaccine advanced horticulture agriculture environment biodiversity conservation science technology communication applications issues

2021 ◽  
Vol 5 (5) ◽  
pp. 211-220
Author(s):  
Subhas Chandra Datta
Keyword(s):  
Biodiversity Conservation ◽  
Systemic Acquired Resistance ◽  
Acquired Resistance ◽  
Foliar Spray ◽  
Fruit Production ◽  
Human Populations ◽  
Vegetable Crops ◽  
Conservation Science ◽  
Resistance Response ◽  
Science Technology

The most ‘Economically-Important Number-One Consumption-Vegetable-Crops,’ is lost by different pathogens like nematodes, causing the root-knot disease which is definitely controlled by different chemical-pesticides, and on the opposite hand, the pandemic coronavirus 2 (SARS-CoV-2) outbreaks of Coronavirus disease 2019 (COVID-19) have emphasized the vulnerability of human populations to novel viral pressures, causing an emergent global pandemic and badly impacts on horticulture-agriculture-environment health socio-economy medical-pharmaceutical science-technology communication issues. So it's an urgent have to develop potential epidemiological and biomedical preventing COVID-19 vaccines. And India emphasis on okra, the ‘Nature's-Gift to Human-Disease-Free-Healthy-Life’, and therefore the ultra-high-diluted biomedicines prepared from okra root, applied and confirmed by foliar spray@ 20 ml/plant each group respectively, are highly effective against the root-knot disease of okra, with increasing fresh-plant growth and fruit production. The high-diluted-biomedicines of okra, are simpler than the untreated ones and show the foremost potential confirmed end in all respects. The genetic-effects of ultra-high-diluted-biomedicines thought to induce systemic acquired resistance response of the treated plants through the expression of pathogenesis-related -proteins-genes (22 to 4 numbers), which are more or less similar molecular range (295kD to 11kD) of the many coronaviruses, and it'll to blame for preventing root-knot and COVID-19 like variant-virus diseases by inducing defense-resistance or increasing innate-immunity, with the toxic-free world, and it should help to develop best potential new preventive treatments methods or drug or vaccines, within the field of ‘21st Century COVID-19 sort of a pandemic within the new normal situation in future, and confirms the “Economic okra Act as a Preventive-COVID-19 Vaccine Advanced Horticulture Agriculture Environment Biodiversity Conservation Science Technology-Communication Applications”, and whole plant act as ‘Nature's-Gift Preventive-COVID-19 Vaccine for All’.

scholarly journals Enriched Agriculture Horticulture Science Technology Socio-EconomyCommunication-Issue by Biomedicines Suppressing Tomato-Disease and Coronavirus 2-Like-Pandemic-Diseases!

2021 ◽  
Vol 4 (2) ◽  
Keyword(s):  
21St Century ◽  
Systemic Acquired Resistance ◽  
Acquired Resistance ◽  
Foliar Spray ◽  
Fruit Production ◽  
Resistance Response ◽  
Science Technology ◽  
Aquatic Science ◽  
Lycopersicon Lycopersicum ◽  
Related Proteins

To avoid traditional control of root-knot disease, there remains a need for developing effective biomedicines of animal origins. The animal biomedicines; Nematode Extract (NE) and Gall Protein (GP) when applied by foliar spray, are highly effective at 1.3mg/plant and 2.01mg/plant, respectively in ameliorating root-knot disease of tomato (Lycopersicon lycopersicum, Cv. Pusa Ruby) caused by Meloidogyne incognita (Kofoid & White) Chitwood, promoting plant growth and fruit production. The nematode extract (NE) is more effective than the gall protein (GP) in this respect. Both the extract is thought to induce systemic acquired resistance response of the treated plants through the expression of pathogenesis-related proteins, and may prevent 21st –century pandemics COVID-19 like virus diseases by boosting immunity, resisting toxic effects on the environment, and improved agriculture green-socio-economy aquatic-science-technology-communication application issues, and it may also give a good scope for new advanced-development and research in the field “Agricultural, Horticulture, Earth-Environmental-Clinical-Toxicology-Health-Research, Science-Technology-Communication-Socio-Economy and May be Controlled 21st-Century Pandemic Diseases in Future New Normal Situation Also”.

scholarly journals Comparison of Methods of Acibenzolar-S-Methyl Application for Post-Infection Fire Blight Suppression in Pear and Apple

Plant Disease ◽  
2016 ◽  
Vol 100 (6) ◽  
pp. 1125-1131 ◽  
Author(s):  
Kenneth B. Johnson ◽  
Todd N. Temple
Keyword(s):  
Fire Blight ◽  
Systemic Acquired Resistance ◽  
Acquired Resistance ◽  
Foliar Spray ◽  
Field Research ◽  
Apple Rootstocks ◽  
Pr Genes ◽  
Treatment Timing ◽  
Guide Field ◽  
Pathogenesis Related

Greenhouse-grown, 1-year-old potted ‘Bosc’ pear and apple rootstock cultivars ‘M.9’ and ‘M.26’ were inoculated with the fire blight pathogen, Erwinia amylovora, and subjected to trunk paint, root drench, or foliar spray treatments with acibenzolar-S-methyl (ASM, 4 to 30 mg a.i./tree) to induce systemic acquired resistance. Each method of ASM treatment suppressed fire blight canker expansion by 22 to 25%. Furthermore, ASM application method and ASM treatment timing (at or ±3 weeks relative to inoculation) interacted significantly (P ≤ 0.02) in each experiment. A root drench was most effective when applied 3 weeks before inoculation (36% suppression) whereas trunk paints and foliar sprays were more effective at inoculation (43 and 34%, suppression, respectively). Sizes of fire blight cankers in potted apple rootstocks M.9 and M.26 (under scions ‘Gala’ or ‘Cameo’) inoculated directly with the pathogen were reduced by 82 and 87% after two pretreatments of ASM applied as a trunk paint or root drench, respectively. Expression of pathogenesis-related (PR) genes PR-1 and -2 in apple leaves sampled after an ASM trunk paint were elevated significantly (P ≤ 0.05) relative to control trees for at least 9 weeks after treatment. Results of this study are being used to guide field research on postinfection therapy with ASM in 1- to 10-year-old pear and apple trees where fire blight has proven difficult to manage with therapeutic pruning only.

scholarly journals Comparative Efficacy of Systemic Acquired Resistance-Inducing Compounds Against Rust Infection in Sunflower Plants

2007 ◽  
Vol 97 (2) ◽  
pp. 179-186 ◽  
Author(s):  
Esther Amzalek ◽  
Yigal Cohen
Keyword(s):  
Systemic Acquired Resistance ◽  
Acquired Resistance ◽  
Foliar Spray ◽  
Racemic Mixture ◽  
In Planta ◽  
Butanoic Acid ◽  
Defense Compounds ◽  
Rust Infection ◽  
Puccinia Helianthi ◽  
Full Protection

Four inducers of systemic acquired resistance (SAR) were examined for their efficacy in controlling rust infection caused by Puccinia helianthi in sunflower plants. Of the four compounds, DL-3-amino-n-butanoic acid (DL-β-aminobutyric acid [BABA]) was the most effective and sodium salicylate (NaSA) was the least effective in protecting against rust. In leaf disk assays, full protection was obtained with BABA at 25 μg/ml, benzodiathiazol-S-methyl ester (BTH) at 100 μg/ml, 2,6-di-chloroisonicotinic acid (INA) at 100 μg/ml, and NaSA at >200 μg/ml. L-2-amino-n-butanoic acid (AABA) was partially effective, whereas N-methyl-BABA and 4-aminobutnoic acid (GABA) were ineffective. The R-enantiomer of BABA, but not the S-enantiomer, was more effective than the racemic mixture. In intact plants, BABA applied as a foliar spray or a root dip, before or after (up to 48 h) inoculation, provided significant protection for 8 days. BTH, INA, and NaSA were less protective and more phytotoxic compared with BABA. BABA did not affect urediospore germination, germ tube growth, appressorial formation, or initial ingress of P. helianthi, but strongly suppressed mycelial colonization in the mesophyll and, consequently, pustule and urediospore formation. No accumulation of defense compounds (phenolics, lignin, or callose) was detected in BABA-treated inoculated or noninoculated plants. This is the first report on the activity of BABA against an obligate Basidomycete pathogen in planta.

scholarly journals Isolation of New Arabidopsis Mutants With Enhanced Disease Susceptibility to Pseudomonas syringae by Direct Screening

Genetics ◽  
1998 ◽  
Vol 149 (2) ◽  
pp. 537-548
Author(s):  
Sigrid M Volko ◽  
Thomas Boller ◽  
Frederick M Ausubel
Keyword(s):  
Pseudomonas Syringae ◽  
Fungal Pathogen ◽  
Systemic Acquired Resistance ◽  
Disease Susceptibility ◽  
Acquired Resistance ◽  
Bacterial Pathogen ◽  
Avirulent Strain ◽  
Resistance Response ◽  
Arabidopsis Mutants ◽  
Pathogen Response

Abstract To identify plant defense components that are important in restricting the growth of virulent pathogens, we screened for Arabidopsis mutants in the accession Columbia (carrying the transgene BGL2-GUS) that display enhanced disease susceptibility to the virulent bacterial pathogen Pseudomonas syringae pv. maculicola (Psm) ES4326. Among six (out of a total of 11 isolated) enhanced disease susceptibility (eds) mutants that were studied in detail, we identified one allele of the previously described npr1/nim1/sai1 mutation, which is affected in mounting a systemic acquired resistance response, one allele of the previously identified EDS5 gene, and four EDS genes that have not been previously described. The six eds mutants studied in detail (npr1-4, eds5-2, eds10-1, eds11-1, eds12-1, and eds13-1) displayed different patterns of enhanced susceptibility to a variety of phytopathogenic bacteria and to the obligate biotrophic fungal pathogen Erysiphe orontii, suggesting that particular EDS genes have pathogen-specific roles in conferring resistance. All six eds mutants retained the ability to mount a hypersensitive response and to restrict the growth of the avirulent strain Psm ES4326/avrRpt2. With the exception of npr1-4, the mutants were able to initiate a systemic acquired resistance (SAR) response, although enhanced growth of Psm ES4326 was still detectable in leaves of SAR-induced plants. The data presented here indicate that eds genes define a variety of components involved in limiting pathogen growth, that many additional EDS genes remain to be discovered, and that direct screens for mutants with altered susceptibility to pathogens are helpful in the dissection of complex pathogen response pathways in plants.

scholarly journals 487 Assessment of Chemical Induction of Acquired Resistance to Phytophthora Fruit Rot in Field-grown Pickling Cucumber

HortScience ◽  
1999 ◽  
Vol 34 (3) ◽  
pp. 529A-529
Author(s):  
Priscilla M. Hockin ◽  
Irvin E. Widders
Keyword(s):  
Systemic Acquired Resistance ◽  
Phytophthora Capsici ◽  
Acquired Resistance ◽  
Lesion Size ◽  
Plant Diseases ◽  
Fruit Rot ◽  
Chemical Induction ◽  
Resistance Response ◽  
Chemical Inducers ◽  
Cucumber Fruit

Systemic acquired resistance (SAR) is a physiological defense response in plants conferring broad spectrum resistance to pathogens. SAR is inducible through infection by necrotizing pathogens or chemical inducers and involves the systemic activation of defense related genes. Our objectives were to evaluate resistance expression to phytophthora soft rot fruit in cucumber in response to increasing concentrations of 2,6 dichloroisonicotinic acid (INA) and benzo (1,2,3)thiadiazole-7-carbothioc acid S-methyl ester (BTH) by foliar applications. Excised leaves exhibited a resistance response to foliar applications of all concentrations of INA and BTH tested when challenge inoculated with Colletotrichum lagenarium. There was increasing benefit with increasing concentration of each chemical applied. Harvested cucumber fruit, 3.4 to 4.5 cm in diameter, were challenge inoculated with Phytophthora capsici; there were no significant chemical and rate interactions in terms of internal lesion measurements. Overall, INA consistently reduced lesion size in cucumber fruit. A bioassay conducted on fruit of different maturity levels, as defined by fruit diameter, revealed that larger sized fruit (4 to 5 cm) were more resistant to fruit rot. Fruit with diameters of 3 to 4 cm from plots treated with BTH showed little resistance as compared to the control and fruit from the same treatment with diameters of 2 to 3 cm. Fruit from plots treated with INA had at least 50% reduction in lesion size than the control. It is unclear if these differences were attributable to changes in physiological or anatomical factors. The true importance of these results should be interpreted with caution. Yield studies have not been conducted, and thus, with the experienced stunting, treatment with 100 ppm INA would be expected to lower yield and perhaps fruit quality. Determination of the optimal application regime and other cultural factors will provide broad control of plant diseases.

Assessment of Chemical Induction of Acquired Resistance to Pythium Fruit Rot in Field-grown Cucumber

HortScience ◽  
1998 ◽  
Vol 33 (3) ◽  
pp. 525d-525 ◽  
Author(s):  
Priscilla M. Hockin ◽  
Irvin E. Widders
Keyword(s):  
Systemic Acquired Resistance ◽  
Isonicotinic Acid ◽  
Acquired Resistance ◽  
Soft Rot ◽  
Fruit Rot ◽  
Chemical Induction ◽  
Resistance Response ◽  
Chemical Inducers ◽  
Cucumber Fruit ◽  
Pythium Soft Rot

Systemic acquired resistance (SAR) is a physiological defense response in plants conferring broad spectrum resistance to pathogens. SAR is inducible through infection by necrotizing pathogens or chemical inducers and involves the systemic activation of defense related genes. The objectives of this study were to evaluate resistance expression to Pythium soft rot in fruit of cucumber in response to foliar applications of 2,6-dichloro isonicotinic acid (INA), benzo(1,2,3)thiadiazole-7-carbothioc acid S-methyl ester (CGA 245704), or beta-amino-butyric acid (bABA). Excised leaves from three cucumber cultivars, Vlaspik, Sumpter and SMR 58, exhibited a resistance response to foliar applications of INA (50 ppm) and CGA 245704 (25 ppm) when challenge inoculated with Colletotrichum lagenarium. However, the reduction in lesion incidence by INA was 99% in Vlaspik and only 11% and 18% in Sumpter and SMR 58, respectively. bABA did not enhance resistance in vegetative tissues. Multiple foliar applications of INA and CGA 245704 prior to fruit set were found to be more effective than a single application. Harvested cucumber fruit, 3.0 to 5.0 cm in diameter, were wounded and challenge inoculated with Pythium sp.; no statistically significant differences in infection were observed between controls and fruit from chemically treated plots. In Vlaspik, however, INA and BTH did reduce the rot infection rate in fruit when foliar applications were made early in plant ontogeny, at the four-leaf stage. In addition, larger more mature fruit (4-5 cm diameter) exhibited slower lesion growth than less developed fruit (>3 cm). It is unclear if these differences were attributable to changes in physiological or anatomical factors. These findings indicate that the level of physiological resistance achievable through chemical induction in cucumber is a function of the specific pathogen and the plant organ being infected, i.e., leaves or fruit.

scholarly journals Arabidopsis thaliana EDS4 Contributes to Salicylic Acid (SA)-Dependent Expression of Defense Responses: Evidence for Inhibition of Jasmonic Acid Signaling by SA

2000 ◽  
Vol 13 (5) ◽  
pp. 503-511 ◽  
Author(s):  
Vaijayanti Gupta ◽  
Michael G. Willits ◽  
Jane Glazebrook
Keyword(s):  
Gene Expression ◽  
Salicylic Acid ◽  
Jasmonic Acid ◽  
Pseudomonas Syringae ◽  
Systemic Acquired Resistance ◽  
Acquired Resistance ◽  
Defense Responses ◽  
Avirulence Gene ◽  
Signal Molecule ◽  
Resistance Response

The Arabidopsis enhanced disease susceptibility 4 (eds4) mutation causes enhanced susceptibility to infection by the bacterial pathogen Pseudomonas syringae pv. Maculicola ES4326 (Psm ES4326). Gene-for-gene resistance to bacteria carrying the avirulence gene avrRpt2 is not significantly affected by eds4. Plants homozygous for eds4 exhibit reduced expression of the pathogenesis-related gene PR-1 after infection by Psm ES4326, weakened responses to treatment with the signal molecule salicylic acid (SA), impairment of the systemic acquired resistance response, and reduced accumulation of SA after infection with Psm ES4326. These phenotypes indicate that EDS4 plays a role in SA-dependent signaling. SA has been shown to have a negative effect on activation of gene expression by the signal molecule jasmonic acid (JA). Two mutations that cause reduced SA levels, eds4 and pad4, cause heightened responses to inducers of JA-dependent gene expression, providing genetic evidence to support the idea that SA interferes with JA-dependent signaling. Two possible working models of the role of EDS4 in governing activation of defense responses are presented.

scholarly journals Effect of Benzothiadiazole on Transmission of X-Disease Phytoplasma by the Vector Colladonus montanus to Arabidopsis thaliana, a New Experimental Host Plant

Plant Disease ◽  
2005 ◽  
Vol 89 (10) ◽  
pp. 1121-1124 ◽  
Author(s):  
Alberto Bressan ◽  
Alexander H. Purcell
Keyword(s):  
Arabidopsis Thaliana ◽  
Systemic Acquired Resistance ◽  
Detrimental Effect ◽  
Acquired Resistance ◽  
Foliar Spray ◽  
Wild Type ◽  
Chain Reaction ◽  
Disease Symptoms ◽  
Phytoplasma Infection ◽  
Polymerase Chain

Colladonus montanus (Van Duzee), a leafhopper vector of X-disease phytoplasma (Xp), efficiently transmitted the pathogen to Arabidopsis thaliana Columbia wild type. During transmission trials, the phytoplasma was inoculated into 22-, 34-, and 40-day-old plants. Phytoplasma infections were confirmed by polymerase chain reaction (PCR) using primers specific for Xp Symptoms in Xp-positive A. thaliana were overall stunting and reduced or no fruit (silques). All symptom-free plants were PCR negative. Leafhopper nymphs free of Xp that fed on diseased A. thaliana acquired and transmitted Xp to celery plants, a diagnostic host, causing typical X-disease symptoms. Foliar spray applications of the plant resistance elicitor benzothiadiazole (BTH) to A. thaliana 1 week before phytoplasma inoculation significantly reduced phytoplasma infection, ranging from an infection rate of 73.7% for untreated plants to 50 and 35% for plants treated with 1.2 and 4.8 mM BTH, respectively. Vector leafhoppers survival was significantly reduced on BTH-treated A. thaliana compared with leafhoppers on nontreated plants, suggesting that systemic acquired resistance in this plant may have some detrimental effect on the leafhopper C. montanus.

scholarly journals Trichoderma asperelloides enhances local and systemic acquired resistance response under low nitrate nutrition in Arabidopsis

2018 ◽  
Author(s):  
Aakanksha Wany ◽  
Pradeep K. Pathak ◽  
Alisdair R Fernie ◽  
Kapuganti Jagadis Gupta
Keyword(s):  
Pseudomonas Syringae ◽  
Systemic Acquired Resistance ◽  
N Uptake ◽  
Acquired Resistance ◽  
Marker Genes ◽  
Defense Gene Expression ◽  
Resistance Response ◽  
Defense Gene ◽  
Protein Levels

AbstractNitrogen (N) is essential for growth, development and defense but, how low N affects defense and the role of Trichoderma in enhancing defense under low nitrate is not known. Low nitrate fed Arabidopsis plants displayed reduced growth and compromised local and systemic acquired resistance responses when infected with both avirulent and virulent Pseudomonas syringae DC3000. These responses were enhanced in the presence of Trichoderma. The mechanism of increased local and systemic acquired resistance mediated by Trichoderma involved increased N uptake and enhanced protein levels via modulation of nitrate transporter genes. The nrt2.1 mutant is compromised in local and systemic acquired resistance responses suggesting a link between enhanced N transport and defense. Enhanced N uptake was mediated by Trichoderma elicited nitric oxide (NO). Low NO producing nia1,2 mutant and nsHb+ over expressing lines were unable to induce nitrate transporters and thereby compromised defense in the presence of Trichoderma under low N suggesting a signaling role of Trichoderma elicited NO. Trichoderma also induced SA and defense gene expression under low N. The SA deficient NahG transgenic line and the npr1 mutant were also compromised in Trichoderma-mediated local and systemic acquired resistance responses. Collectively our results indicated that the mechanism of enhanced plant defense under low N mediated by Trichoderma involves NO, ROS, SA production as well as the induction of NRT and marker genes for systemic acquired resistance.One-sentence summaryTrichoderma enhances local and systemic acquired resistance under low nitrate nutrition

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