scholarly journals Immobilization of Horseradish Peroxidase on Multi-Armed Magnetic Graphene Oxide Composite

2019 ◽  
Vol 57 (2) ◽  
pp. 260-271 ◽  
Author(s):  
Junhui Rong ◽  
Yun Wang ◽  
Liang Ni ◽  
Wenli Zhang ◽  
Chunmei Li ◽  
...  
Keyword(s):  
Graphene Oxide ◽  
Horseradish Peroxidase ◽  
Degradation Rate ◽  
Magnetic Composite ◽  
Support Material ◽  
Amino Groups ◽  
Magnetic Graphene Oxide ◽  
Magnetic Graphene ◽  
Loading Amount ◽  
First Time

In this study, a novel type of multi-armed polymer (poyltehylene glycol, PEG) magnetic graphene oxide (GO) composite (GO@Fe3O4@6arm-PEG-NH2) has been synthesized as a support for immobilization of horseradish peroxidase (HRP) for the first time. The loading amount of HRP was relatively high (186.34 mg/g) due to the surface of carrier material containing a large amount of amino groups from 6arm-PEG-NH2, but degradation rate of phenols was also much higher (95.4 %), which is attributed to the synergistic effect between the free HRP (45.4 %) and the support material of GO@Fe3O4@6arm-PEG-NH2 (13.6 %). Compared with the free enzyme, thermal, storage and operational stability of the immobilized HRP improved. The immobilized HRP still retained over 68.1 % activity after being reused 8 times. These results suggest that the multi-armed magnetic composite has good application prospect for enzyme immobilization.

The fabrication of dendrimeric phenylboronic acid-functionalized magnetic graphene oxide nanoparticles with excellent adsorption performance for the separation and purification of horseradish peroxidase

2020 ◽  
Vol 44 (14) ◽  
pp. 5254-5264
Author(s):  
Youyuan Xing ◽  
Juan Han ◽  
Lei Wang ◽  
Chunmei Li ◽  
Jiacong Wu ◽  
...  
Keyword(s):  
Graphene Oxide ◽  
Horseradish Peroxidase ◽  
Phenylboronic Acid ◽  
Oxide Nanoparticles ◽  
Separation And Purification ◽  
Magnetic Graphene Oxide ◽  
Adsorption Performance ◽  
Magnetic Graphene ◽  
Oxide Nanoparticle

A dendrimeric phenylboronic acid-affinitive magnetic graphene oxide nanoparticle was synthesized and used to separate and purify HRP.

Adsorption behavior of Cr(vi) from aqueous solution onto magnetic graphene oxide functionalized with 1,2-diaminocyclohexanetetraacetic acid

RSC Advances ◽  
2015 ◽  
Vol 5 (56) ◽  
pp. 45384-45392 ◽  
Author(s):  
Fang-ying Guo ◽  
Yun-guo Liu ◽  
Hui Wang ◽  
Guang-ming Zeng ◽  
Xin-jiang Hu ◽  
...  
Keyword(s):  
Aqueous Solution ◽  
Graphene Oxide ◽  
Adsorption Behavior ◽  
Magnetic Composite ◽  
Magnetic Graphene Oxide ◽  
Composite Adsorbent ◽  
Magnetic Graphene

A novel magnetic composite adsorbent was synthesized by grafting 1,2-diaminocyclohexanetetraacetic acid to magnetic graphene oxide (DCTA/E/MGO).

Development of A Novel System Based on Green Magnetic / Graphene Oxide / Chitosan /Allium Sativum / Quercus / Nanocomposite for Targeted Release of Doxorubicin Anti-Cancer Drug

2020 ◽  
Vol 20 (9) ◽  
pp. 1094-1104 ◽  
Author(s):  
Omid Arjmand ◽  
Mehdi Ardjmand ◽  
Ali M. Amani ◽  
Mohmmad H. Eikani
Keyword(s):  
Graphene Oxide ◽  
Allium Sativum ◽  
Cytotoxicity Assay ◽  
Targeted Treatment ◽  
Cancer Drug ◽  
Magnetic Graphene Oxide ◽  
Magnetic Graphene ◽  
Anti Cancer ◽  
Cancerous Cells ◽  
Targeted Release

Background: Doxorubicin, as a strong anti-cancer agent for clinical treatment of various cancer types along with other drugs, is widely utilized. Due to the physiology of the human body and cancerous tissues, the applicability of doxorubicin is still limited and the targeted treatment of the different types of cancers is considered. Also, the side effects of the conventional forms of chemotherapy medicines, damaging and stressing the normal cells are considerable. Objective: This study introduces a novel and effective system for the targeted release of doxorubicin by successfully fabricating the green magnetic graphene oxide, chitosan, allium sativum, and quercus nanocomposite. Methods: The in vitro release of doxorubicin loaded on the nanocomposite was evaluated and investigated at pH 7.4 and 6.5, respectively. The drug diffusivity in the plasma environment was assessed for a more accurate analysis of the drug diffusion process. The nanocomposite loaded drug release mechanism and kinetics, as well as cytotoxicity assay was investigated. Results: The efficiency of the drug encapsulation was significantly enhanced using natural extract ingredients and consequently, the efficacy of the targeted treatment of cancerous tissues was improved. The developed nanocomposite provided a controlled release of doxorubicin in similar acidic conditions of the normal and cancerous cells and affirming that the fabricated system is thoroughly pH-dependent. Conclusion: The cytotoxicity assay confirmed that the fabricated nanocomposite at a high growth rate of cancerous cells has an anticancer property and acts as a toxic agent against tumor cells, suggesting that in conjunction with doxorubicin, it can be highly improved for killing cancerous cells.

scholarly journals Magnetization of graphene oxide nanosheets using nickel magnetic nanoparticles as a novel support for the fabrication of copper as a practical, selective, and reusable nanocatalyst in C–C and C–O coupling reactions

RSC Advances ◽  
2021 ◽  
Vol 11 (42) ◽  
pp. 25867-25879
Author(s):  
Parisa Moradi ◽  
Maryam Hajjami
Keyword(s):  
Graphene Oxide ◽  
Catalytic Activity ◽  
Magnetic Nanoparticles ◽  
Coupling Reactions ◽  
Graphene Oxide Nanosheets ◽  
Magnetic Graphene Oxide ◽  
Diaryl Ethers ◽  
Magnetic Graphene

Catalytic activity of supported copper on magnetic graphene oxide was investigated as a selective and reusable nanocatalyst in the synthesis of diaryl ethers and biphenyls.

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