scholarly journals Effect of a long-term peroral supplementation with sodium selenite and selenium lactate-protein complex on selenium status in goats and their kids

2009 ◽  
Vol 54 (No. 7) ◽  
pp. 324-332 ◽  
Author(s):  
L. Misurova ◽  
L. Pavlata ◽  
A. Pechova ◽  
R. Dvorak
Keyword(s):  
Glutathione Peroxidase ◽  
Protein Complex ◽  
Sodium Selenite ◽  
Selenium Concentration ◽  
Selenium Supplementation ◽  
Control Group ◽  
Glutathione Peroxidase Activity ◽  
Significant Difference ◽  
Average Activity

The aim of this study was to evaluate the effect of a long-term peroral selenium supplementation in the form of sodium selenite and selenium lactate-protein complex by comparing selenium concentrations and glutathione peroxidase activity in blood of goats and their kids as well as comparing selenium concentrations in goat colostrums. For the study, a total of 27 clinically healthy pregnant white shorthair goats were used. They were divided to three groups, i.e., the control group (C) without any selenium supplementation, sodium selenite group (E1) and selenium lactate-protein complex group (E2). For four months, experimental goats received 0.43 mg of selenium per animal per day in diet; goats from the control group were given 0.15 mg of selenium per animal per day. At the beginning of the experiment, goats of all groups showed an average selenium concentration of 96 &mu;g/l in whole blood. On the parturition day, samples of first colostrum from goats and heparinized blood from goats and kids were taken. In the control group (C), average blood selenium concentrations of 111.4 ± 33.5 &mu;g/l were observed on the parturition day. In both experimental groups, selenium concentrations were significantly higher (<I>P</I> < 0.05). Average selenium concentration in the sodium selenite group (E1) was 177.2 ± 34.8 &mu;g/l and in the group supplemented with selenium lactate-protein complex (E2) 159.0 ± 28.5 &mu;g/l. Average glutathione peroxidase (GSH-Px) activity in blood of control goats (C) was 581.9 ± 99.2 &mu;kat/l, in group E1 1 154.6 ± 156.2 &mu;kat/l and in group E2 1 011.6 ± 153.6 &mu;kat/l. GSH-Px activity in experimental groups was significantly higher (<I>P</I> < 0.05) as compared with the control group. Average selenium concentrations in colostrum was in the control group 40.1 ± 12.8 &mu;g/l, in E1 99.0 ± 29.9 &mu;g/l and in group E2 79.0 ± 17.7 &mu;g/l. Colostral selenium concentrations in experimental groups were significantly higher (<I>P</I> < 0.05) as compared with the control group. No significant difference in the monitored parameters was found between experimental groups. In kids of control mothers (kC), average selenium concentrations in blood on the parturition day were 62.4 ± 22.9 &mu;g/l; kids of mothers supplemented with sodium selenite (kE1) showed average selenium levels of 100.0 ± 31.2 &mu;g/l, and the average selenium concentration in kids of mothers receiving lactate-protein complex was 83.4 ± 20.1 &mu;g/l (kE2). Average GSH-Px activity in control kids (kC) was 402.1 ± 153.9 &mu;kat/l. Kids from kE1 showed average activity of GSH-Px 806.1 ± 254.9 &mu;kat/l and kids from group kE2 529.9 ± 119.8 &mu;kat/l. Statistically significant difference (<I>P</I> < 0.05) was found only between kC and kE1 which showed significantly higher selenium concentration and GSH-Px activity. The results of this study confirm that both forms of selenium administered in experimental groups (i.e., sodium selenite and selenium lactate-protein complex) had similar biological effect in goats. However, results obtained in kids indicate a better effect of supplementation with sodium selenite.

scholarly journals Impact of Supplementation of Various Selenium Forms in Goats on Quality and Composition of Milk, Cheese and Yoghurt

2008 ◽  
Vol 77 (3) ◽  
pp. 407-414 ◽  
Author(s):  
A. Pechová ◽  
B. Janštová ◽  
L. Mišurová ◽  
M. Dračková ◽  
L. Vorlová ◽  
...  
Keyword(s):  
Lactobacillus Acidophilus ◽  
Protein Complex ◽  
Selenium Concentration ◽  
Selenium Supplementation ◽  
Time Span ◽  
The Other ◽  
Control Group ◽  
Milk Products ◽  
Lactating Goats

The objective of this experiment was to monitor the possibility of influencing the composition of milk and milk products by supplementing the feed of lactating goats with selenium in various forms (natrium selenite, selenium proteinate). The trial was carried out in 30 goats, divided into 3 groups: Group C (n = 10) was a control group, SeI group (n = 10) received selenium in the form of natrium selenite and SeO group (n = 10) in the form of lactate-protein complex produced by Lactobacillus acidophilus. Diverse selenium supplementations were carried out from 3.5–4 months before the date of delivery until the 2nd month of lactation. In the time span of 2 weeks, milk from individual goats was taken six times and cheeses and yoghurts were made from the mixed milk from individual groups. During the period of milking and making milk products, the goats in experimental groups received added 565 μg Se per animal and day in relevant forms. Long-term selenium supplementation in the form of natrium selenite/proteinate had no significant effect on the Se concentration in milk. Se concentration in milk of individual groups was as follows: C: 13.14 ± 4.21 μg l-1, SeI: 12.50 ± 5.59 μg l-1, SeO: 12.47 ± 3.71 μg l-1. Similarly, no significant differences in the selenium concentration were determined in yoghurts (C: 17.13 ± 5.40 μg kg-1, SeI: 18.12 ± 5.49 μg kg-1, SeO: 19.69 ± 6.48 μg kg-1) and cheeses (C: 68.79 ± 7.14 μg kg-1, SeI: 70.41 ± 6.22 μg kg-1, SeO: 69.66 ± 12.68 μg kg-1) made from milk of individual goats groups. Apart from selenium concentration in milk and milk products, we also monitored other quality indicators that might have been influenced by selenium supplementation in the goats' feed. Among qualitative milk indicators, we found significantly lower titration acidity and significantly higher rennetability in SeI group. We also determined a significantly higher fat concentration in milk of SeO group compared to the other two groups. Indicators monitored during production of yoghurts and cheeses were not influenced significantly, only fat concentration in yoghurts was higher in SeO group compared to SeI group. Supplementation of selenium in the form of natrium selenite and selenium proteinate exceeding the physiological needs of goats had no significant effect on the Se concentration in milk and milk products.

scholarly journals Comparison of organic and inorganic forms of selenium in the mother and kid relationship in goats

2012 ◽  
Vol 57 (No. 8) ◽  
pp. 361-369 ◽  
Author(s):  
L. Pavlata ◽  
L. Mišurová ◽  
A. Pechová ◽  
R. Dvořák
Keyword(s):  
Glutathione Peroxidase ◽  
Whole Blood ◽  
Sodium Selenite ◽  
Dry Matter ◽  
Control Group ◽  
Individual Trial ◽  
Significant Difference ◽  
The Individual ◽  
Se Status

The goal of the experiment was to compare the effect of four different forms of selenium (Se) &minus; sodium selenite (SS), lactate-protein selenium complex (SL), selenium enriched yeast (SY), and selenium-proteinate (SP) supplemented to pregnant goats on Se concentration and glutathione peroxidase (GSH-Px) activity in the blood of goats on the day of delivery and also on Se concentration and GSH-Px activity in the blood of newborn kids. The experiment involved 33 pregnant goats of White Short-haired breed. The supplementation started 6 weeks before the parturition. The goats were divided into 5 groups: control group&nbsp;C, not supplemented, and 4 trial groups (SL, SP, SS, SY), which received Se in the above stated forms by the means of supplemented pellets (300 g per animal per day) at a rate 900 &mu;g Se/kg of dry matter. The average Se concentrations in the blood of the goats were 79.6 &mu;g/l in group C, 152.6 &mu;g/l in group SL, 167.1&nbsp;&mu;g/l in group SP, 144.9 &mu;g/l in group SS, and 152.9 &mu;g/l in group SY. Selenium concentrations in all 4 trial groups were significantly higher (P &lt; 0.01) than in control group, however no significant difference was found between individual trial groups. Likewise, the activity of GSH-Px in goat blood increased significantly in all supplemented groups compared to the controls; however we did not discover any significant differences in activity of GSH-Px between the individual selenium-supplemented groups. The Se concentrations in the blood of kids were significantly (P &lt; 0.01) higher in the selenium-supplemented groups (SL &ndash; 94.9&nbsp;&mu;g/l, <br />SP &ndash; 87.5 &mu;g/l, SS &ndash; 87.6 &mu;g/l, SY &ndash; 92.5 &mu;g/l) than in the control group (C &ndash; 49.4 &mu;g/l), but we did not discover any differences between the individual experimental groups. The activity of GSH-Px in the blood of the kids tended towards higher values in the supplemented groups than in the control group, but the values were significantly higher (P &lt; 0.05) only in groups SY and SL. We have found significant correlation between GSH-Px activity and Se concentration in the blood of goats (r = 0.86) and newborn kids (r = 0.95). Likewise, there was significant correlation between Se concentration in the blood of goats and their kids (r&nbsp;= 0.74). We discovered that the kids are reaching physiologically only about 60% of Se status in whole blood in comparison with their mothers. Our results are suggesting that all the above forms of Se were similarly utilised and transferred into the foetus in the goats. &nbsp;

scholarly journals Nano-selenium Supplementation Increases Selenoprotein (Sel) Gene Expression Profiles and Milk Selenium Concentration in Lactating Dairy Cows

2020 ◽  
Vol 199 (1) ◽  
pp. 113-119
Author(s):  
Liqiang Han ◽  
Kun Pang ◽  
Tong Fu ◽  
Clive J. C. Phillips ◽  
Tengyun Gao
Keyword(s):  
Gene Expression ◽  
Glutathione Peroxidase ◽  
Dairy Cows ◽  
Sodium Selenite ◽  
Expression Profiles ◽  
Gene Expression Profiles ◽  
Selenium Concentration ◽  
Basal Diet ◽  
Control Group ◽  
Lactation Performance

AbstractSupplementation with selenium is common for dairy cows, but the importance of selenium source is not clear. This study aimed to compare nano-selenium (Nano-Se) and sodium selenite supplements for dairy cows on lactation performance, milk Se levels and selenoprotein (Sel) gene expression. Twelve multiparous Holstein cows were randomly divided into two groups: a control group fed a basal diet plus 0.30 mg Se/kg of DM as sodium selenite or Nano-Se for 30 days. Dry matter intake, milk yield and composition were not affected by dietary Se source (P > 0.05); however, the milk total Se levels and milk glutathione peroxidase (GSH-Px) activities were higher with Nano-Se supplementation than sodium selenite (P < 0.05). At the end of the experiment, Nano-Se supplementation significantly increased plasma Se levels and GSH-Px activity, compared with the sodium selenite supplement. The mRNA expression levels of glutathione peroxidase 1, 2 and 4; thioredoxin reductase 2 and 3; and selenoproteins W, T, K and F were markedly upregulated (P < 0.05) in the mammary gland of the Nano-Se group. Thus, the source of selenium plays an important role in the antioxidant status and in particular the Sel gene expression in the mammary glands of dairy cows, both being stimulated by nano sources.

scholarly journals Effect of a single, oral administration of selenitetriglycerides, at two dose rates, on blood selenium status and haematological and biochemical parameters in Holstein-Friesian calves

2021 ◽  
Vol 74 (1) ◽  
Author(s):  
Katarzyna Żarczyńska ◽  
Przemysław Sobiech ◽  
Dawid Tobolski ◽  
John F. Mee ◽  
Josef Illek
Keyword(s):  
Glutathione Peroxidase ◽  
Peroxidase Activity ◽  
Biochemical Parameters ◽  
Selenium Concentration ◽  
Biologically Active ◽  
Control Group ◽  
Serum Selenium ◽  
Selenium Status ◽  
Glutathione Peroxidase Activity ◽  
Holstein Friesian

Abstract Background Selenitetriglycerides are biologically active, organic forms of selenium formed as a result of the modification of selenic acid and sunflower oil. Studies in rats have shown that they are well absorbed and of low toxicity. There are no published studies on selenitetriglycerides supplementation in calves. Results In this study, selenitetriglycerides were administered once orally on the 2nd day of life at a dose of 0.5 or 1 mg Se/kg body weight to each of six Holstein-Friesian calves while six control calves were not supplemented. Blood for determination of selenium concentration, glutathione peroxidase activity, haematological parameters, aspartate aminotransferase, creatine kinase, and lactate dehydrogenase activities and glucose, total protein, albumin, triglycerides, cholesterol, urea, and creatinine concentration was collected before supplementation (day 0) and 1, 2, 5, 10 and 14 days after supplementation. Selenitetriglycerides administration increased (P < 0.01) serum selenium concentration in supplemented calves as early as day1, from a mean of 63.4 to 184.22 µg/l in calves receiving selenium at a dose of 0.5 mg/kg BW, and from 63.17 to 200.33 µg/l in calves receiving 1 mg/kg. Serum selenium concentrations remained significantly higher compared to the control group throughout the experiment. Glutathione peroxidase activity was higher in supplemented than control calves, significantly so in animals receiving the 1 mg/kg dose of Se on the 10th and 14th days (P < 0.05). There were no significant differences in the haematological and biochemical parameters between the groups. Conclusions This experiment showed that supplementation with selenitetriglycerides could significantly improve blood selenium status in calves without adverse effects on haematological or biochemical parameters. These findings are essential prerequisites for future studies on selenitetriglycerides supplementation to manage clinical selenium deficiency in calves.

scholarly journals Activity of glutathione peroxidase (GSH-Px) in the blood of ewes and their lambs receiving the selenium-enriched unicellular alga Chlorella

2008 ◽  
Vol 53 (No. 7) ◽  
pp. 292-298 ◽  
Author(s):  
J. Trávníček ◽  
J. Racek ◽  
L. Trefil ◽  
H. Rodinová ◽  
V. Kroupová ◽  
...  
Keyword(s):  
Glutathione Peroxidase ◽  
Blood Serum ◽  
Whole Blood ◽  
Selenium Supplementation ◽  
Control Group ◽  
Organic Form ◽  
Pregnancy And Lactation ◽  
Average Activity ◽  
Positive Effect ◽  
Experimental Group

The effect of supplementation of selenium inorganic and organic forms on the activity of glutathione peroxidase (GSH-Px) was investigated for 7–8 months in three groups of ewes (by five animals each) and in born lambs. The basal feed ration contained 55 &mu;g Se, the ewes of experimental group E1 received a supplement of 180 &mu;g Se in sodium selenite, and the ewes of experimental group E2 were administered a supplement of 180 &mu;g selenium bound in the biomass of the alga <I>Chlorella</I>. Control group C was without selenium supplement. The ewes were in the stage of non-pregnancy, pregnancy and lactation during the experiment. The average number of lambs born per ewe was 1.0 in groups C and E1 and 1.8 in group E2. Both forms of selenium supplementation resulted in higher activity of GSH-Px in the whole blood, higher content of Se in the blood serum (<I>P</I> < 0.001) and milk of ewes (<I>P</I> < 0.001). Average activity of GSH-Px in the whole blood of ewes was as follows: C 697.9 ± 179.3; E1 1 147.4 ± 181.5; E2 1 056.1 ± 267.5 U/g Hb (<I>P</I> < 0.001). It reached the highest values in the 5<sup>th</sup> to 6<sup>th</sup> month of Se supplementation while the activity decreased after parturition in connection with lactation. Higher utilisation of Se from the organic form, compared to the inorganic form, was reflected in a higher content of Se in the milk of group E2 ewes (<I>P</I> < 0.05). The positive effect of selenium supplementation of ewes contributed to higher activity of GSH-Px in the whole blood of their lambs (<I>P</I> < 0.001) and higher Se concentration in the blood serum (<I>P</I> < 0.01). The organic form of Se (group E2) was more efficient in this case. Average activity of GSH-Px in the whole blood of lambs was as follows: C 434.1 ± 70.6; E1 1 031.6 ± 172.3 and E2 1 055.6 ± 235.1 U/g Hb.

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