scholarly journals Comparison of organic and inorganic forms of selenium in the mother and kid relationship in goats

2012 ◽  
Vol 57 (No. 8) ◽  
pp. 361-369 ◽  
Author(s):  
L. Pavlata ◽  
L. Mišurová ◽  
A. Pechová ◽  
R. Dvořák
Keyword(s):  
Glutathione Peroxidase ◽  
Whole Blood ◽  
Sodium Selenite ◽  
Dry Matter ◽  
Control Group ◽  
Individual Trial ◽  
Significant Difference ◽  
The Individual ◽  
Se Status

The goal of the experiment was to compare the effect of four different forms of selenium (Se) &minus; sodium selenite (SS), lactate-protein selenium complex (SL), selenium enriched yeast (SY), and selenium-proteinate (SP) supplemented to pregnant goats on Se concentration and glutathione peroxidase (GSH-Px) activity in the blood of goats on the day of delivery and also on Se concentration and GSH-Px activity in the blood of newborn kids. The experiment involved 33 pregnant goats of White Short-haired breed. The supplementation started 6 weeks before the parturition. The goats were divided into 5 groups: control group&nbsp;C, not supplemented, and 4 trial groups (SL, SP, SS, SY), which received Se in the above stated forms by the means of supplemented pellets (300 g per animal per day) at a rate 900 &mu;g Se/kg of dry matter. The average Se concentrations in the blood of the goats were 79.6 &mu;g/l in group C, 152.6 &mu;g/l in group SL, 167.1&nbsp;&mu;g/l in group SP, 144.9 &mu;g/l in group SS, and 152.9 &mu;g/l in group SY. Selenium concentrations in all 4 trial groups were significantly higher (P &lt; 0.01) than in control group, however no significant difference was found between individual trial groups. Likewise, the activity of GSH-Px in goat blood increased significantly in all supplemented groups compared to the controls; however we did not discover any significant differences in activity of GSH-Px between the individual selenium-supplemented groups. The Se concentrations in the blood of kids were significantly (P &lt; 0.01) higher in the selenium-supplemented groups (SL &ndash; 94.9&nbsp;&mu;g/l, <br />SP &ndash; 87.5 &mu;g/l, SS &ndash; 87.6 &mu;g/l, SY &ndash; 92.5 &mu;g/l) than in the control group (C &ndash; 49.4 &mu;g/l), but we did not discover any differences between the individual experimental groups. The activity of GSH-Px in the blood of the kids tended towards higher values in the supplemented groups than in the control group, but the values were significantly higher (P &lt; 0.05) only in groups SY and SL. We have found significant correlation between GSH-Px activity and Se concentration in the blood of goats (r = 0.86) and newborn kids (r = 0.95). Likewise, there was significant correlation between Se concentration in the blood of goats and their kids (r&nbsp;= 0.74). We discovered that the kids are reaching physiologically only about 60% of Se status in whole blood in comparison with their mothers. Our results are suggesting that all the above forms of Se were similarly utilised and transferred into the foetus in the goats. &nbsp;

scholarly journals Effect of a long-term peroral supplementation with sodium selenite and selenium lactate-protein complex on selenium status in goats and their kids

2009 ◽  
Vol 54 (No. 7) ◽  
pp. 324-332 ◽  
Author(s):  
L. Misurova ◽  
L. Pavlata ◽  
A. Pechova ◽  
R. Dvorak
Keyword(s):  
Glutathione Peroxidase ◽  
Protein Complex ◽  
Sodium Selenite ◽  
Selenium Concentration ◽  
Selenium Supplementation ◽  
Control Group ◽  
Glutathione Peroxidase Activity ◽  
Significant Difference ◽  
Average Activity

The aim of this study was to evaluate the effect of a long-term peroral selenium supplementation in the form of sodium selenite and selenium lactate-protein complex by comparing selenium concentrations and glutathione peroxidase activity in blood of goats and their kids as well as comparing selenium concentrations in goat colostrums. For the study, a total of 27 clinically healthy pregnant white shorthair goats were used. They were divided to three groups, i.e., the control group (C) without any selenium supplementation, sodium selenite group (E1) and selenium lactate-protein complex group (E2). For four months, experimental goats received 0.43 mg of selenium per animal per day in diet; goats from the control group were given 0.15 mg of selenium per animal per day. At the beginning of the experiment, goats of all groups showed an average selenium concentration of 96 &mu;g/l in whole blood. On the parturition day, samples of first colostrum from goats and heparinized blood from goats and kids were taken. In the control group (C), average blood selenium concentrations of 111.4 ± 33.5 &mu;g/l were observed on the parturition day. In both experimental groups, selenium concentrations were significantly higher (<I>P</I> < 0.05). Average selenium concentration in the sodium selenite group (E1) was 177.2 ± 34.8 &mu;g/l and in the group supplemented with selenium lactate-protein complex (E2) 159.0 ± 28.5 &mu;g/l. Average glutathione peroxidase (GSH-Px) activity in blood of control goats (C) was 581.9 ± 99.2 &mu;kat/l, in group E1 1 154.6 ± 156.2 &mu;kat/l and in group E2 1 011.6 ± 153.6 &mu;kat/l. GSH-Px activity in experimental groups was significantly higher (<I>P</I> < 0.05) as compared with the control group. Average selenium concentrations in colostrum was in the control group 40.1 ± 12.8 &mu;g/l, in E1 99.0 ± 29.9 &mu;g/l and in group E2 79.0 ± 17.7 &mu;g/l. Colostral selenium concentrations in experimental groups were significantly higher (<I>P</I> < 0.05) as compared with the control group. No significant difference in the monitored parameters was found between experimental groups. In kids of control mothers (kC), average selenium concentrations in blood on the parturition day were 62.4 ± 22.9 &mu;g/l; kids of mothers supplemented with sodium selenite (kE1) showed average selenium levels of 100.0 ± 31.2 &mu;g/l, and the average selenium concentration in kids of mothers receiving lactate-protein complex was 83.4 ± 20.1 &mu;g/l (kE2). Average GSH-Px activity in control kids (kC) was 402.1 ± 153.9 &mu;kat/l. Kids from kE1 showed average activity of GSH-Px 806.1 ± 254.9 &mu;kat/l and kids from group kE2 529.9 ± 119.8 &mu;kat/l. Statistically significant difference (<I>P</I> < 0.05) was found only between kC and kE1 which showed significantly higher selenium concentration and GSH-Px activity. The results of this study confirm that both forms of selenium administered in experimental groups (i.e., sodium selenite and selenium lactate-protein complex) had similar biological effect in goats. However, results obtained in kids indicate a better effect of supplementation with sodium selenite.

scholarly journals The application of a selenium fertiliser for the correction of marginal deficiencies in grazing sheep

1999 ◽  
Vol 70 (3) ◽  
Author(s):  
S.W.P. Cloete ◽  
F.E. Van Niekerk ◽  
M. Young ◽  
G.D. Van der Merwe ◽  
J. Clark
Keyword(s):  
Whole Blood ◽  
Sodium Selenite ◽  
Dry Matter ◽  
Liver And Kidney ◽  
Poorly Soluble ◽  
Se Deficiency ◽  
Ram Lambs ◽  
Grazing Sheep ◽  
Lamb Growth ◽  
Se Status

A commercial fertiliser, consisting of a poorly soluble barium selenate core with a coating of highly soluble sodium selenite, was evaluated in 2 trials for the provision of selenium (Se) to grazing sheep. The fertiliser was administered at a level of 1 kg per hectare to 3 of 6 kikuyu paddocks during 1995 and 1996 in Trial 1, while the other paddocks were left untreated. The Se status of SA mutton merino ram lambs, as reflected by whole blood, liver and kidney Se concentrations, was elevated (P<0.01) for at least 5 months after application of the fertiliser. Whole blood and liver Se concentrations of animals grazing unfertilised control paddocks were indicative of a subclinical Se deficiency at times (<100 ng Se/mℓ whole blood and <300 mg Se/kg liver dry matter). In Trial 2, 4 of 7 paddocks on which an oat fodder crop was established were treated with the Se fertiliser during 1995 and 1997. The remaining 3 paddocks were left unfertilised as controls. Groups of 10-15 pregnant SA mutton merino ewes were introduced to these paddocks within 2 weeks of parturition. These ewes and their progeny utilised these paddocks for a mean (+SD) period of 41+8 days after parturition. The whole blood Se concentrations of these ewes and their offspring were elevated (P < 0.01) relative to their contemporaries utilising control paddocks. No suggestion of a subclinical Se deficiency was discernible in animals grazing control paddocks, although whole blood Se levels approached 100 ng Se/mℓ during 1997. The application of Se fertiliser did not result in improvements in ewe reproduction or lamb growth. There was a suggestion of an improvement (P = 0.21) in mean (+SE) lamb survival on paddocks receiving Se fertiliser compared to control paddocks (71.5 + 4.6 % vs 62.2 + 5.3 % respectively).

scholarly journals The effect of inorganic and organically bound forms of selenium on glutathione peroxidase activity in the blood of goats

2011 ◽  
Vol 56 (No. 2) ◽  
pp. 75-81 ◽  
Author(s):  
L. Pavlata ◽  
L. Misurova ◽  
A. Pechova ◽  
R. Dvorak
Keyword(s):  
Glutathione Peroxidase ◽  
Lactobacillus Acidophilus ◽  
Whole Blood ◽  
Sodium Selenite ◽  
Complex Form ◽  
Biologically Active ◽  
Glutathione Peroxidase Activity ◽  
The Difference ◽  
Se Status

The goal of the experiment was to compare the effect of supplementation of inorganic and the new organically bound (lactate-protein selenium complex) form of selenium (Se) in feed for goats. The 31 goats were split into three groups: control (C) without Se supplementation, AN group administered sodium selenite, ORG group administered lactate-protein selenium complex (Selene Chelate, Agrobac, Czech Republic) produced by cultivation of Lactobacillus acidophilus on a substrate containing natrium selenite. The total Se intake in goats was 0.15 mg in group C, and 0.43 mg in the groups AN and ORG. The effect of Se supplementation was assessed based on the determination of Se concentration and the activity of glutathione peroxidase (GSH-Px) in whole blood. Samples were taken before the beginning of Se supplementation, 14 and 30 days after the start of supplementation, and then two and three months after the beginning of supplementation. Average Se concentrations in the blood of goats in individual groups (C, AN, ORG) before the start of supplementation were 109.6 &plusmn; 34.3, 117.5 &plusmn; 34.7, and 105.4 &plusmn; 43.6 &mu;g/l respectively, and the activity of GSH-Px in whole blood was 745.3 &plusmn; 289.2, 810.7 &plusmn; 280.4, and 791.0 &plusmn; 398.1 &mu;kat/l respectively. While in group C goats neither the Se concentration nor the GSH-Px activity changed substantially during the experiment, in the goats in the experimental groups there was a statistically significant increase (P &lt; 0.01) in both Se concentrations and the GSH-Px activities. At the end of the experiment Se concentrations in the blood of AN and ORG groups amounted to 168.5 &plusmn; 12.2 and 168.8 &plusmn; 26.8 &mu;g/l. The GSH-Px activities in goats supplemented with Se also increased significantly over the course of the experiment (at the end of the experiment it was 1178.0 &plusmn; 127.3 in the AN group and 1030.1 &plusmn; 152.3 &mu;kat/l in the ORG group), and the difference between the groups was significant (P = 0.038). Regarding the dynamics of GSH-Px activity changes during the monitored period, a markedly quicker increase in GSH-Px activity was recorded in the AN group &ndash; one month after the beginning of Se supplementation, compared to three months after the beginning of Se supplementation in the ORG group. The results thus show that the effects of supplementation with selenite and the lactate-protein selenium complex are similar with regard to Se status, but that the increase in GSH-Px activity occurred much faster with selenite, which therefore appears to be a more biologically available form of selenium for creation of biologically active selenoproteins.

THE EFFECT OF CAFFEINE ON SHORT-TERM SPATIAL LEARNING IN PERIPLANETA AMERICANA

2010 ◽  
Vol 6 ◽  
Author(s):  
Evan D Broder ◽  
Kristen M Cattoi ◽  
William B Clinkscales ◽  
Taylor S Triana
Keyword(s):  
Treatment Group ◽  
Spatial Learning ◽  
Periplaneta Americana ◽  
T Test ◽  
Control Group ◽  
Short Term ◽  
Individual Trial ◽  
Significant Difference ◽  
The Individual ◽  
Time Required

The American cockroach, Periplaneta americana, has been used extensively in recent years to study aspects of the complex vertebrate nervous system as a simple and accessible invertebrate model. This experiment examined the effects of caffeine on short-term spatial learning in cockroaches using a modified Barnes maze. Prior to every trial, the treatment group was injected with a caffeine solution and the control group was injected with a saline solution. The amount of time required for the cockroach to complete the maze during the initial learning trial was recorded. One hour later, the amount of time required for the subjects to complete a second memory trial was also recorded. A one-tailed t-test between the individual trial times for the control group established that learning had occurred. A two-tailed t¬-test between the individual trial times for the treatment group showed no significant difference. A t-test of means compared the difference in maze completion times between the groups and proved there was a significant difference. Therefore, caffeine did not positively affect short-term spatial learning in cockroaches.

Viscosity and Red Cell Deformity in Arterial Disease

1979 ◽  
Author(s):  
G Cella ◽  
H de Haas ◽  
M Rampling ◽  
V Kakkar
Keyword(s):  
Vascular Disease ◽  
Blood Viscosity ◽  
Whole Blood ◽  
Arterial Disease ◽  
Plasma Viscosity ◽  
Control Group ◽  
Fibrinogen Concentration ◽  
Red Cell ◽  
Whole Blood Viscosity ◽  
Significant Difference

Haemorrheological factors have been shown to be affected in many kings of vascular disease. The present study was undertaken to correlate these factors in normal subjects and patients suffering from peripheral arterial disease. Twenty-two patients were investigated; they had moderate or severe intermittent claudication, extent of disease being confirmed by aorto-arteriography and ankle-systolic pressure studies. Twenty-five controls with no symptoms or signs of arterial disease were selected with comparable age and sex distribution. Whole blood viscosity was measured at shear rates of 230 secs-1 and 23 secs-lat 37°c using a Wells Brookfield cone plate microvisco meter. Plasma viscosity was also measured in an identical manner. Erythrocyte flexibility was measured by centrifuge technique and fibrinogen concentration as well as haematocrit by standard techniques. The fibrinogen concentration appeared to be the only significant parameter; the mean concentration in patients with peripheral vascular disease of 463 ± 73mg/l00ml in the control group ( < 0.05). Although whole blood viscosity was high in patients, when corrected to a common haematocrit, there was no significant difference between patients and controls. The same megative correlation was found for plasma viscosity. The red cell flexibility was found to be increased in patients as compared to the control group, but this effect appeared to be simply proportional to the fibrinogen concentration.

scholarly journals ESTILO DE VIDA E SUAS RELAÇÕES COM HIPERTENSÃO ARTERIAL SISTÊMICA E DIABETES MELLITUS

2018 ◽  
Vol 7 (1) ◽  
pp. 361-373
Author(s):  
Elias Ferreira Porto ◽  
Claudia Kumpel ◽  
Anselmo Cordeiro de Souza ◽  
Izabel Maria de Oliveira ◽  
Karoline Mayara de Aquiles Bernardo ◽  
...  
Keyword(s):  
Diabetes Mellitus ◽  
General Health ◽  
Healthy Lifestyle ◽  
Cross Sectional Study ◽  
Health Perception ◽  
Control Group ◽  
Healthy Individuals ◽  
Cross Sectional ◽  
Significant Difference ◽  
The Individual

Avaliar o estilo de vida e percepção do estado geral de saúde em pacientes com Diabetes Mellitus tipo 2 - DM, Hipertensão Arterial Sistêmica - HAS e indivíduos saudáveis. Métodos: Estudo transversal, com grupo controle. Avaliado estilo de vida com Questionário Fantástico, e percepção geral de saúde via uma pergunta âncora de diabéticos do tipo 2 (n = 37), hipertensos (n = 60), e indivíduos saudáveis (n = 43). Análise estatística descritiva, analise de variância, e razão de chance (Odds Rattio – OR). Resultados: Verificou-se diferença significante (p0,0001) na pontuação do Questionário Fantástico entre os indivíduos saudáveis em relação DM e HAS. O pior desempenho no estilo de vida para os três grupos foi nos domínios de atividade física e alimentação. O risco do indivíduo com HAS afirmar que sua saúde é pior do que indivíduos da mesma faixa etária foi OR 1.8 (1.415 a 2.419) e para os indivíduos diabéticos OR de 2.8 (1.776 a 4.579) em relação a indivíduos saudáveis. Conclusão: Diabéticos e hipertensos têm um pior estilo de vida e percepção geral de saúde do que indivíduos saudáveis. Assim o estilo de vida saudável pode reduzir a prevalência e auxiliar no controle de doenças já estabelecidas.Palavras-chave: Estilo de vida. Hipertensão Arterial. Diabetes Mellitus. ABSTRACT: To evaluate the lifestyle and perception of general health status in patients with type 2 diabetes mellitus - DM, systemic arterial hypertension - SAH and healthy individuals. Methods: Cross-sectional study with control group. (N = 37), hypertensive (n = 60), and healthy subjects (n = 43) were assessed using a Fantastic Questionnaire, and general health perception via an anchor question. Descriptive statistical analysis, analysis of variance, and odds ratio (Odds Rattio - OR). Results: There was a significant difference (p 0.0001) in the score of the Fantastic Questionnaire among healthy individuals in relation to DM and SAH. The worst lifestyle performance for the three groups was in the physical activity and feeding domains. The risk of the individual with SAH to state that their health is worse than individuals of the same age group was OR 1.8 (1.415 to 2.419) and for the diabetic individuals OR of 2.8 (1.776 to 4.579) in relation to healthy individuals. Conclusion: Diabetics and hypertensives have a worse lifestyle and overall health perception than healthy individuals. Thus the healthy lifestyle can reduce the prevalence and help in the control of already established diseases.Keywords: Lifestyle. Hypertension. Diabetes Mellitus.

scholarly journals Impact of long-term supplementation of zinc and selenium on their content in blood and hair in goats

2011 ◽  
Vol 56 (No. 2) ◽  
pp. 63-74 ◽  
Author(s):  
L. Pavlata ◽  
M. Chomat ◽  
A. Pechova ◽  
L. Misurova ◽  
R. Dvorak
Keyword(s):  
Blood Plasma ◽  
Whole Blood ◽  
Average Concentration ◽  
The Other ◽  
Control Group ◽  
Selenium Yeast ◽  
Hair Samples ◽  
The Impact ◽  
Se Status

This paper evaluates the impact of long-term supplementation of different forms of zinc (Zn) and selenium (Se) on the content of these substances in the blood and hair of goats. Two analogous supplementation experiments were performed. 37 goats divided into four groups were used in the first trial with the Zn supplementation. Group A (n = 10) was a control group (with no Zn administered). A further three groups (B, C, D) were supplemented with Zn in various forms. Group B (n = 9) with zinc oxide, Group C (n = 9) with zinc lactate and Group D (n = 9) with zinc chelate. The second trial with Se supplementation was carried out on 20 goats divided into four groups. Group E (n = 5) was a control group. The other three groups were administered Se. Group F (n = 5) was supplied with a selenium lactate-protein complex, Group G (n = 5) with sodium selenite and Group H (n = 5) with selenium yeast. Three months later blood and hair samples were taken from all animals and Zn and Se concentrations were determined in whole blood, plasma, and hair. Glutathione peroxidase (GSH-Px) activity was determined in the Se supplementation trial group. At the end of the trial the Zn concentrations in plasma and whole blood were without major differences between the groups. The plasma concentration of Zn did not increase from the initial value at the start of the trial. In hair the average concentration of Zn was 95.2&ndash;100.0 mg/kg<br />in all groups. No conclusive relation was confirmed between the values of Zn in hair and its concentration in blood. The Se concentration in whole blood (&micro;g/l) at the end of trial in supplemented groups (F &ndash; 188.8 &plusmn; 24.6; G &ndash; 197.2 &plusmn; 10.9; H &ndash; 190.1 &plusmn; 26.3) was significantly higher (P &lt; 0.01) than in the control group (E &ndash; 103.1 &plusmn; 23.5). Similarly, the activity of GSH-Px (&micro;kat/l) was significantly higher in all supplemented groups (F &ndash; 872.3 &plusmn; 94.8; G &ndash; 659.5 &plusmn; 176.4; H &ndash; 839.8 &plusmn; 150.8) than in the control group (E &ndash; 379.1 &plusmn; 63.5). Se content in hair (&micro;g/kg) was higher also in all trial groups (F &ndash; 242.3 &plusmn; 41.5; G &ndash; 200.5 &plusmn; 46.9; H &ndash; 270.0 &plusmn; 106.8) than in the control group (E &ndash; 174.7 &plusmn; 38.0). However, it was significantly (P &lt; 0.05) higher only in Group F. A conclusive correlation was identified between the Se concentration in whole blood and its content in hair (r = 0.54; P &lt; 0.05; n = 20). Based on the results it can be concluded that none of the supplemented forms of Zn increased its concentration in blood, plasma and hair. On the other hand, the administration of Se led to an increase in the Se concentration in blood, increased the activity of GSH-Px in whole blood and the Se content in hair. Based on the proven correlation and regression relation between the Se concentration in blood and its content in hair, hair can be considered as a suitable material for the diagnosis of long-term Se status in goats. Goats with sufficient Se status are those that have more than 160 &micro;g/kg of Se in hair dry weight.

scholarly journals INFLUENCE OF ACCEPTANCE AND COMMITMENT THERAPY ON THE FAMILY’S BURDEN IN TREATING PEOPLE WITH MENTAL DISORDERS

2017 ◽  
Vol 2 (2) ◽  
pp. 126
Author(s):  
I Putu Gde Yudara Sandra Putra ◽  
Noor Hamdani ◽  
Lilik Supriati
Keyword(s):  
Mental Disorders ◽  
Linear Regression ◽  
Acceptance And Commitment Therapy ◽  
Control Group ◽  
Commitment Therapy ◽  
Acceptance And Commitment ◽  
Significant Difference ◽  
The Family ◽  
New Perspective ◽  
The Individual

Indonesia has more than one million people with mental disorders spread in each province. Treatment for people with schizophrenia is not only done in the hospital alone, but it will be continued after returning to the family. Families who are not able to adapt to the patient's care problem will consider the problem as a burden by the family. Acceptance and Commitment Therapy (ACT) is one of therapy that aims to teach the individual to be aware and use a new perspective in thinking to face a problem. This research aimed to know the effect of ACT on the burden felt by the family in caring for the patient mental disorders. The design used in this research was Quasi-Experiment Pre-Post Test With Control Group. The numbers of samples in this study were 24 people divided into two groups. The independent variable in this study was ACT therapy while the dependent variable was the burden felt by the family. Data analysis in this research use t-test and linear regression. The results showed no significant difference in the control group after getting health counseling (p = 1.000). In the treatment group showed significant difference after getting health education and ACT (p = 0.000). There was a significant difference between intervention and control group (p = 0.000). The result of linear regression test showed that age factor and ACT therapy had an effect of 57.1% to the family burden. Families are expected to be more flexible to face problems found when treating people with mental disorders at home.

scholarly journals Evaluation of Anti-oxidant Enzymes, Lipid Peroxidation, Lipid Profile and Liver Function in Albino Rats Orally Administered Tartrazine

2020 ◽  
pp. 19-29
Author(s):  
Uyota Anthony Adele ◽  
Geraldine Iroh ◽  
Ojoye Ngoye Briggs ◽  
Helen Anthony Waribo ◽  
Ibioku Elekima
Keyword(s):  
Lipid Peroxidation ◽  
Liver Function ◽  
Lipid Profile ◽  
Whole Blood ◽  
Test Group ◽  
Control Group ◽  
Albino Rats ◽  
Central Vein ◽  
Significant Difference ◽  
Anti Oxidant

Aim: To evaluate the anti-oxidant enzymes, lipid peroxidation, lipid profile and liver function in albino rats orally administered tartrazine. Study Design: A total number of 63 female albino rats weighing approximately 0.2 kg were used for this study. The study was divided into two phases, phase 1 which lasted for the first 30 days, comprised of 35 rats, 20 rats were used as test group while 15 rats served as the control group. Phase 2 of the study was for 60 days and 28 rats were used with 16 as test group and 12 as the control. The test groups were orally administered with 7.5 mg/kg of tartrazine (ADI) daily over the specified periods while the control groups were not treated with tartrazine but given only food and water. Place and Duration of Study: The study was carried out in the Department of Medical Laboratory Science, Rivers State University, Port Harcourt, Nigeria within a period of 12 months (Feb., 2019 – Jan., 2020). Methodology: At the end of the study, 5 mls of whole blood specimens was collected by means of cardiac puncture into plain bottles. To obtain the serum, the whole blood samples were allowed to clot and later dislodged and spun at 3500 rpm for 10 minutes. The collected serum specimens were used to analyze SOD, MDA, GPX, ALT, GGT, ALP, TG, TCHOL, and HDL-C, while LDL-C was calculated using Friedwald equation. Results: The chronic treatment of rats with tartrazine azo food dye at the ADI dose caused an increase in MDA levels after 30 and 60 days test rats compared to the control, while TCHOL and HDL-C showed significant decrease after 30 and 60 days of treatment in the test group compared to the control group. In addition, ALT indicated significant increase in test group after 60 days of treatment compared to control group. ALP, GGT, TG, LDL-C, SOD and GPX showed no significant difference after 30, and 60 days of treatment at ADI doses. Histologic examination of the liver indicated hydropic dilation, degenerating hepatocyes and infiltration of central vein with parenchymal materials alongside kupffer cells. Conclusion: The results from this study revealed that orally administered tartrazine at the recommended ADI dose increased lipid peroxidation as seen in the elevated MDA levels. Hepatic derangements were also seen as revealed by increased ALT and histologic distortions as well fall in TCHOL and HDL-C lipid fractions.

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