scholarly journals Use of Nuclear Microsatellite Loci for Evaluating Genetic Diversity of Selected Populations of Picea abies (L.) Karsten in the Czech Republic

Forests ◽  
2018 ◽  
Vol 9 (2) ◽  
pp. 92 ◽  
Author(s):  
◽  
◽  
Keyword(s):  
Genetic Diversity ◽  
Czech Republic ◽  
Picea Abies ◽  
Microsatellite Loci ◽  
The Czech Republic ◽  
Nuclear Microsatellite

scholarly journals Polymorphism of prolamin proteins of the grain of triticale varieties certifiedin the Czech Republic

2011 ◽  
Vol 51 (No. 4) ◽  
pp. 151-155 ◽  
Author(s):  
T. Vyhnánek ◽  
J. Bednář
Keyword(s):  
Genetic Diversity ◽  
Czech Republic ◽  
Cold Resistance ◽  
The Czech Republic ◽  
Baking Quality ◽  
The Individual

 Genetic diversity was detected in 11 varieties of triticale registered in the Czech Republic by means of polymorphism of prolamin proteins using the PAGE ISTA method. The polymorphism of prolamin proteins allowed the differentiation of the individual triticale varieties in 2002 and 2003 harvests. On the basis of Dice’s calculations of coefficients of similarity we discovered, in parallel with the uniform genotypes, genotypes with sister prolamin spectrums with a different percentage of participation in the respective years. A uniform spectrum was detected in the following varieties: Disco, Kolor, Lamberto, Marko, Presto, Sekundo, Ticino and Tricolor; Kitaro and Modus were dimorphous varieties. In 2003 three sister prolamin lines appeared in the variety Gabo and in 2004 only two. In 2003 a 5% admixture of a foreign genotype was detected in the variety Marko. Typical of the unknown genotype was the gliadin block Gld 1B3, which is the marker of rye translocation T1BL.1RS, gene Sr31 with resistance to black rust, higher cold resistance and lower baking quality of the wheat. The prolamin proteins of triticale grain are suitable for the detection of the genetic diversity and for the assessment of varietal authenticity and purity in seed samples of triticale varieties registered in the Czech Republic.

scholarly journals Testing of germination of spruce, pine and larch seed after 10 years from collection

2014 ◽  
Vol 60 (No. 12) ◽  
pp. 540-543
Author(s):  
I. Tomášková ◽  
J. Vítámvás ◽  
J. Korecký
Keyword(s):  
Czech Republic ◽  
Picea Abies ◽  
Pinus Sylvestris ◽  
Tree Species ◽  
Abiotic Factors ◽  
Forest Tree ◽  
Germination Capacity ◽  
The Czech Republic ◽  
Germination Energy ◽  
Seed Ageing

:Germination capacity and germination energy are usually the most frequently used quantitative parameters of forest tree seed. With seed ageing both parameters decreased and the rate of the collapse is given by tree species, age of tree and its seed and biotic and abiotic factors. Relatively little attention has been paid to the age of seed. As it was found, the longevity of the main tree species remained relatively high, and spruce (Picea abies [L.] Karsten and pines (Pinus sylvestris L.) from the investigated areas across the Czech Republic maintained minimally one third of germination capacity or germination energy during the 10 years with the exception of larch (Larix decidua Mill.) where germination capacity decreased almost to zero after 10 years. Although the germination energy and germination capacity decreased significantly, it is possible to use the seed in the case of shortage of the seed of better quality.  

scholarly journals Molecular genetic diversity and relationship of indigenous sheep breeds of Pakistan based on nuclear microsatellite loci

2019 ◽  
Vol 30 (1) ◽  
pp. 54
Author(s):  
T Hussain ◽  
M M. Musthafa ◽  
M E. Babar ◽  
M Shaheen ◽  
F M. Marikar
Keyword(s):  
Genetic Diversity ◽  
Microsatellite Loci ◽  
Molecular Genetic ◽  
Sheep Breeds ◽  
Indigenous Sheep ◽  
Molecular Genetic Diversity ◽  
Nuclear Microsatellite ◽  
Relationship Of

<p>En Pakistán los recursos genéticos ovinos son abundantes, disponiéndose de varias diferentes castas extendidas en todo el país. No obstante, la diversidad molecular de las razas de ovejas es poco conocida en la región. Por ello, en el presente estudio se investigaron 16 marcadores microsatelitales de las razas indígenas Buchi y Hashtnagri, en las provincias de Pendjab y Balochistan respectivamente. Para indagar la diversidad genética se obtuvieron muestras de sangre de 25 animales de cada raza. El promedio del número de alelos en Buchi y Hashtnagri fue de 3,375±1,455 y 3,50±1,591 respectivamente. Los promedios de heterocigosis registradas fueron de 0,878±0,204 para la raza Buchi y 0,885±0,218 para Hashtnagri. Los índices de Shannon fueron 1,032±0,371 para Buchi y 1,070±0,412 para Hashtnagri, respectivamente. La estimación de los coeficientes de endogamia (FIS y FIT) mostró valores negativos, en tanto que el flujo genético (migración) fue de 10,09 y el promedio de la diferencia poblacional (FST) resultó de 2,4%. En ambas razas, el índice de información polimórfica fue 0,56, indicando el valor del marcador del tablero. Entre las ovejas Buchi y Hashtnagri, la distancia genética estándar de Nei (Ds) fue 0,0218. Según estos resultados, ambas razas mostraron considerable diversidad genética. Los datos obtenidos permiten vislumbrar una promisoria mejoría en el área de la conservación y en el diseño de las estrategias para la cría de ovejas en el futuro cercano.</p>

scholarly journals Comparative analysis of genetic diversity in Norway spruce (Picea abies) clonal seed orchards and seed stands

2021 ◽  
Vol 49 (4) ◽  
pp. 12575
Author(s):  
Elena CIOCÎRLAN ◽  
Neculae ȘOFLETEA ◽  
Georgeta MIHAI ◽  
Maria TEODOSIU ◽  
Alexandru L. CURTU
Keyword(s):  
Genetic Diversity ◽  
Comparative Analysis ◽  
Picea Abies ◽  
Norway Spruce ◽  
Allelic Richness ◽  
Conifer Species ◽  
Seed Orchards ◽  
Eastern Carpathians ◽  
Nuclear Microsatellite ◽  
Planted Tree

Norway spruce, Picea abies (L.) Karst. is the most important conifer species in Romania and the most planted tree species in the Carpathian Mountains. Here we compare the genetic diversity of four Norway spruce clonal seed orchards and two seed stands located in the Eastern Carpathians. A set of highly polymorphic nuclear microsatellite markers was used. The analysis of genotypic identity of ramets for each Norway spruce clone in all seed orchards indicated that nearly all sampled ramets (97%) were genetically identical. The genetic diversity in seed orchards (He=0.700) was slightly smaller compared to the seed stands (He=0.718). Allelic richness was higher in seed stands (10.874), compared to clonal seed orchards (8.941). The Bayesian analysis indicated a genetic structure with two clusters, one corresponding to the clonal seed orchards and a second one consisting of the two seed stands. Our results provide valuable information for the management of Norway spruce seed orchards in Romania.

Microsatellite-based genetic diversity and differentiation of foreign common carp (Cyprinus carpio) strains farmed in the Czech Republic

Aquaculture ◽  
2010 ◽  
Vol 298 (3-4) ◽  
pp. 194-201 ◽  
Author(s):  
Martin Hulak ◽  
Vojtech Kaspar ◽  
Klaus Kohlmann ◽  
Kevin Coward ◽  
Jakub Tešitel ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Czech Republic ◽  
Common Carp ◽  
Cyprinus Carpio ◽  
The Czech Republic ◽  
Common Carp Cyprinus Carpio ◽  
Carp Cyprinus Carpio

scholarly journals Characterization of Czech hop (Humulus lupulus L.) genotypes by molecular methods

2011 ◽  
Vol 48 (No. 8) ◽  
pp. 343-350 ◽  
Author(s):  
J. Patzak
Keyword(s):  
Genetic Diversity ◽  
Czech Republic ◽  
Humulus Lupulus ◽  
Quality System ◽  
Molecular Methods ◽  
Germplasm Management ◽  
Genetic Studies ◽  
The Czech Republic ◽  
Humulus Lupulus L

In the last year, new hybrid hop (Humulus lupulus L.) variety Agnus was released for cultivation in the Czech Republic. It has been necessary to prepare the quality system of Agnus identification from other Czech genotypes and characterise the germplasm of this variety by molecular methods. We proved that utilization of five STS primer combinations successfully and completely identified and determined Czech released varieties and new promising breeding materials. The use of STS method was also very effective and sensitive for control of authenticity and purity of variety Agnus in multipropagation cycle. The study of genetic diversity of 61 hop varieties by RAPD, STS, ISSR and AFLP methods confirmed, that germplasm of variety Agnus has ranked among high-alfa varieties. The results can be successfully used for identification, germplasm management, genetic studies and breeding purposes by breeders, multipropagators and hop growers.

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