scholarly journals The use of molecular markers for characterisation of spring barley for breeding to Fusarium head blight resistance

2011 ◽  
Vol 51 (No. 11) ◽  
pp. 483-490 ◽  
Author(s):  
M. Špunarová ◽  
J. Ovesná ◽  
L. Tvarůžek ◽  
L. Kučera ◽  
J. Špunar ◽  
...  
Keyword(s):  
Fusarium Head Blight ◽  
Spring Barley ◽  
Fusarium Head Blight Resistance ◽  
Malting Quality ◽  
F1 Hybrids ◽  
Malt Extract ◽  
Head Blight ◽  
Breeding Lines ◽  
Susceptible Line ◽  
Dh Lines

Fusarium head blight (FHB) is a barley disease, which occurs every year in various areas of barley cultivation all over the world and the increasing incidence has been confirmed in the Czech Republic also during the last years. We aimed to emply AFLP (Amplified fragment length polymorphism) and SSR (Single sequence repeats) markers to describe diversity among breeding lines with a sufficient level of resistance towards FHB and to find marker(s) associated with the analysed traits. The number of eight accessions including five expected resistance donors and three sensitive lines were tested in the field and laboratory. The field values and the amount of deoxynivalenol were positively correlated (r = 0.92). The laboratory test and content DON manifested also a high correlation (r = 0.73). Several DH lines developed from androgenetic barley progenies of the F1 hybrids between the susceptible line PI 383933 and resistant line PEC 210 or the susceptible line PI 383933 and resistant cultivar Chevron were found resistant towards Fusarium infection in both the field and laboratory tests. Low infestation was found at line DH 37 from combination Chevron × PI 383933 and lines DH48, DH49, DH50 and DH55 from the combination PEC 210 × PI 383933. Cluster analyses based on 68 AFLP and 18 SSR markers demonstrate a genetic relationship among parental genotypes and DH lines.Some DH lines combined a sufficient degree of resistance against FHB and extract content (basic parameters malting quality). Statistically significant differences in malt-extract values were observed between groups of the DH lines possessing and not possessing the AFLP marker CAA/AGC 341bp. The markers will be further evaluated and optionally used for MAS.

scholarly journals Epigenetic regulation of gene expression improves Fusarium head blight resistance in durum wheat

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Jitendra Kumar ◽  
Krishan M. Rai ◽  
Seyedmostafa Pirseyedi ◽  
Elias M. Elias ◽  
Steven Xu ◽  
...  
Keyword(s):  
Fusarium Head Blight ◽  
Regulation Of Gene Expression ◽  
Fusarium Head Blight Resistance ◽  
Mapk Signaling ◽  
Head Blight ◽  
Breeding Lines ◽  
Significant Difference ◽  
Plant Pathogen Interaction ◽  
Fhb Resistance ◽  
Parental Lines

Abstract Eight advanced durum-breeding lines were treated with 5-methyl-azacytidine to test the feasibility of generating sources of Fusarium head blight (FHB) resistance. Of the 800 treated seeds, 415 germinated and were advanced up to four (M4) generations by selfing. Thirty-two of the resulting 415 M4 lines were selected following preliminary screening and were further tested for FHB resistance for three years at two field locations, and in the greenhouse. Five of the 32 M4 lines showed less than 30% disease severity, as compared to the parental lines and susceptible checks. Fusarium-damaged kernels and deoxynivalenol analyses supported the findings of the field and greenhouse disease assessments. Two of the most resistant M4 lines were crossed to a susceptible parent, advanced to third generation (BC1:F3) and were tested for stability and inheritance of the resistance. About, one third of the BC1:F3 lines showed FHB resistance similar to their M4 parents. The overall methylation levels (%) were compared using FASTmC method, which did not show a significant difference between M4 and parental lines. However, transcriptome analysis of one M4 line revealed significant number of differentially expressed genes related to biosynthesis of secondary metabolites, MAPK signaling, photosynthesis, starch and sucrose metabolism, plant hormone signal transduction and plant-pathogen interaction pathways, which may have helped in improved FHB resistance.

scholarly journals Evaluation of Canadian barley breeding lines for Fusarium head blight resistance

2015 ◽  
Vol 95 (5) ◽  
pp. 923-929 ◽  
Author(s):  
Xinyao He ◽  
Mohamed Osman ◽  
James Helm ◽  
Flavio Capettini ◽  
Pawan K. Singh
Keyword(s):  
Fusarium Head Blight ◽  
Fusarium Head Blight Resistance ◽  
Correlation Coefficients ◽  
Blight Resistance ◽  
Head Blight ◽  
Breeding Programs ◽  
Breeding Lines ◽  
Barley Breeding ◽  
Days To Heading ◽  
Fhb Resistance

He, X., Osman, M., Helm, J., Capettini, F. and Singh, P. K. 2015. Evaluation of Canadian barley breeding lines for Fusarium head blight resistance. Can. J. Plant Sci. 95: 923–929. Fusarium head blight (FHB) is a major challenge to the successful production of barley in Canada, as well as for end-users such as the malting and brewing industries. Due to the quantitative inheritance of FHB resistance, continuous effort is required to identify breeding lines with improved FHB resistance and incorporate them into crossing schemes to enhance FHB resistance. In the present study, 402 advanced breeding lines from Alberta, Canada, were evaluated in the FHB screening nursery at CIMMYT, Mexico. In 2011 and 2012, FHB incidence was measured on a scale of 1 to 4 to eliminate the most susceptible lines. In 2013 and 2014, 181 lines with the lowest disease scores in the previous 2 yr were tested in replicated experiments for field FHB index, Fusarium-damaged kernels, and deoxynivalenol content. Agronomic and morphological traits, specifically days to heading, plant height, and row and hull types were also evaluated in relations to FHB parameters. Correlation coefficients among the three FHB parameters in both 2013 and 2014 were all significant at P<0.0001, ranging from 0.36 to 0.63. Additional correlation analysis showed that late-maturing, tall, and two-row lines tended to have lower disease, whereas hull type did not show a significant correlation with FHB. Several lines with high and stable FHB resistance similar to that of the resistant checks were identified. These could be used in breeding programs as resistance sources or be registered as new cultivars if their overall attributes meet commercial standards.

Inhibition of deoxynivalenol translocation and fungal colonization in Fusarium head blight resistant wheat

1992 ◽  
Vol 70 (8) ◽  
pp. 1570-1576 ◽  
Author(s):  
C. H. A. Snijders ◽  
C. F. Krechting
Keyword(s):  
Fusarium Head Blight ◽  
Fungal Biomass ◽  
Fusarium Head Blight Resistance ◽  
Reliable Estimate ◽  
Fungal Colonization ◽  
Minor Role ◽  
Breeding Line ◽  
Resistant Line ◽  
Head Blight ◽  
Susceptible Line

Ergosterol analyses of seed samples infected with Fusarium culmorum from 22 wheat genotypes differing in resistance to Fusarium head blight indicated that visual assessment of head blight levels from a field plot gives a reliable estimate of the amount of fungal biomass. The percentage of infected spikelets was a better indication of the amount of fungal biomass present than the percentage of infected heads. This suggests that Fusarium head blight resistance in this population is mainly based on resistance to colonization and that resistance to establishment of the initial infection plays only a minor role. Plants from a susceptible and a resistant breeding line were inoculated with F. culmorum at flowering time. Four weeks after inoculation heads were selected with Fusarium-infected spikelets levels ranging from 0 to 100%. At this time only low levels of ergosterol were detected in the kernels. Eight weeks after inoculation, ergosterol concentrations in chaff and kernels were significantly higher in the susceptible line compared with the resistant breeding line. At 4 and 8 weeks after inoculation the trichothecene deoxynivalenol content of chaff and kernels was also much higher in the susceptible line than in the resistant line. The Fusarium head blight resistant line possesses resistance to hyphal invasion. It appears that deoxynivalenol is transported from the chaff to the young kernel and the pathogen then colonizes the kernel. In the resistant line deoxynivalenol translocation from chaff to kernel was inhibited and little fungal colonization occurred. It is hypothesized that this is based on a membrane-based trichothecene tolerance. Key words: wheat, Fusarium, trichothecene, resistance, ergosterol, deoxynivalenol.

Multiplexed PCR to screen for a major QTL carrying fusarium head blight resistance in Sumai-3 wheat

2006 ◽  
Vol 86 (3) ◽  
pp. 711-716 ◽  
Author(s):  
M. A. Matus-Cádiz ◽  
C. J. Pozniak ◽  
G. R. Hughes ◽  
P. Hucl
Keyword(s):  
Fusarium Head Blight ◽  
Fusarium Head Blight Resistance ◽  
Triticum Aestivum L ◽  
Wheat Breeding ◽  
Head Blight ◽  
Breeding Lines ◽  
Multiplexed Pcr ◽  
Trait Locus ◽  
Advanced Generation ◽  
Sumai 3

After the initial identification of microsatellites linked to economically important traits of interest, the additional investment to simplify the screening procedure for more routine use is of interest to plant breeders. The objective was to simplify an acrylamide gel based marker-assisted selection (MAS) method to facilitate high-through put screening for Qfhs.ndsu-3BS, a major quantitative trait locus carrying fusarium head blight (FHB) resistance in Sumai-3 wheat (Triticum aestivum L.). Method simplifications included incorporating the use of (1) 96-well DNA extractions, (2) multiplexed PCR reactions using microsatellite primers gwm493 and gwm533, and (3) agarose gels. Our modified FHB-MAS method was verified by screening six resistant (Sumai-3, ND2710, McVey, BacUp, HY644, and Alsen) and 52 susceptible parents and subsequently used to screen 5567 common wheat breeding lines developed from Sumai-3 derivatives. This simple and rapid method allows for the screening of 1000 lines per week, which can be used to skew segregating populations towards more resistant types and thereby advance primarily early and advanced generation lines that carry at least the major FHB QTL. Key words: PCR, Fusarium, microsatellites, gwm493, gwm533, Triticum

scholarly journals Evaluation of spring barley genotypes with different susceptibility to Fusarium head blight using molecular markers

2011 ◽  
Vol 52 (No. 11) ◽  
pp. 485-491 ◽  
Author(s):  
Z. Nesvadba ◽  
T. Vyhnánek ◽  
I. Ježíšková ◽  
L. Tvarůžek ◽  
M. Špunarová ◽  
...  
Keyword(s):  
Fusarium Head Blight ◽  
Rapd Markers ◽  
Spring Barley ◽  
Rapd Marker ◽  
Laboratory Evaluation ◽  
Head Blight ◽  
Specific Product ◽  
Dh Lines ◽  
Moderately Resistant ◽  
Barley Genotypes

One of cereal diseases that has gained a greater importance for growers, processing industry as well as for breeders in many regions of the world is Fusarium head blight (FHB) caused by fungi of the Fusarium genus. The objectives of this study were to test diversity among spring barley breeding lines exhibiting various sensitivity to FHB and to find RAPD markers and AFLP markers that will distinguish between susceptible and resistant and/or moderately resistant genotypes. A test of a set of spring barley genotypes artificially infected by fusaria in field trials was carried out. Based on the results from field and laboratory evaluation and deoxynivalenol (DON) content assessment, barley genotypes with different responses to FHB were selected. The genotypes were hybridized and doubled haploid (DH) lines were derived in F<sub>1</sub> generation using the in vitro androgenesis method. Initial parental components and derived DH lines were tested for FHB infection and DON content. A set of parental genotypes of spring barley was tested with 80 RAPD markers. A RAPD marker (H30) was detected which enabled to distinguish between very susceptible parental genotypes and other resistant or moderately resistant spring barley genotypes based on the fragment of about 1300 bp. This specific product was screened in 23 DH lines derived from crosses of parental genotypes of spring barley and detected in 10 DH lines. During the study, some DH lines were selected that exhibited improved resistance to Fusarium infection. A low infection level and low DON content was found in the line DH&nbsp;4/2 derived from CI 4196 &times; Foster. The AFLP technique was used to analyse parental genotypes of spring barley. The detected markers can be further evaluated and employed to select breeding materials.

scholarly journals Merging Genomics and Transcriptomics for Predicting Fusarium Head Blight Resistance in Wheat

Genes ◽  
2021 ◽  
Vol 12 (1) ◽  
pp. 114
Author(s):  
Sebastian Michel ◽  
Christian Wagner ◽  
Tetyana Nosenko ◽  
Barbara Steiner ◽  
Mina Samad-Zamini ◽  
...  
Keyword(s):  
Molecular Markers ◽  
Fusarium Head Blight ◽  
Fusarium Head Blight Resistance ◽  
Genomic Data ◽  
Single Step ◽  
Head Blight ◽  
Step Method ◽  
Prediction Ability ◽  
Breeding Lines ◽  
Cost Efficient

Genomic selection with genome-wide distributed molecular markers has evolved into a well-implemented tool in many breeding programs during the last decade. The resistance against Fusarium head blight (FHB) in wheat is probably one of the most thoroughly studied systems within this framework. Aside from the genome, other biological strata like the transcriptome have likewise shown some potential in predictive breeding strategies but have not yet been investigated for the FHB-wheat pathosystem. The aims of this study were thus to compare the potential of genomic with transcriptomic prediction, and to assess the merit of blending incomplete transcriptomic with complete genomic data by the single-step method. A substantial advantage of gene expression data over molecular markers has been observed for the prediction of FHB resistance in the studied diversity panel of breeding lines and released cultivars. An increase in prediction ability was likewise found for the single-step predictions, although this can mostly be attributed to an increased accuracy among the RNA-sequenced genotypes. The usage of transcriptomics can thus be seen as a complement to already established predictive breeding pipelines with pedigree and genomic data, particularly when more cost-efficient multiplexing techniques for RNA-sequencing will become more accessible in the future.

scholarly journals Fusarium Head Blight Resistance in U.S. Winter Wheat Cultivars and Elite Breeding Lines

Crop Science ◽  
2013 ◽  
Vol 53 (5) ◽  
pp. 2006-2013 ◽  
Author(s):  
Feng Jin ◽  
Dadong Zhang ◽  
William Bockus ◽  
P. Stephen Baenziger ◽  
Brett Carver ◽  
...  
Keyword(s):  
Winter Wheat ◽  
Fusarium Head Blight ◽  
Fusarium Head Blight Resistance ◽  
Blight Resistance ◽  
Wheat Cultivars ◽  
Head Blight ◽  
Breeding Lines ◽  
Winter Wheat Cultivars ◽  
Elite Breeding Lines

Comparing Genetic Variation within Red Winter Wheat Populations with and without Image-Based Optical Sorter Selection

2019 ◽  
Vol 9 (2) ◽  
pp. 266-277
Author(s):  
Hussein M. Khaeim ◽  
Anthony Clark ◽  
Tom Pearson ◽  
Dr. David Van Sanford
Keyword(s):  
Genetic Variation ◽  
Fusarium Head Blight ◽  
Heading Date ◽  
Fusarium Head Blight Resistance ◽  
Gibberella Zeae ◽  
Mass Selection ◽  
Head Blight ◽  
Red Winter Wheat ◽  
Two Populations ◽  
Head Scab

Head scab is historically a devastating disease affecting not just all classes of wheat but also barley and other small grains around the world. Fusarium head blight (FHB), or head scab, is caused most often by Fusarium graminearum (Schwabe), (sexual stage – Gibberella zeae) although several Fusarium spp. can cause the disease. This study was conducted to determine the effect of mass selection for FHB resistance using an image-based optical sorter. lines were derived from the C0 and C2 of two populations to compare genetic variation within populations with and without sorter selection. Our overall hypothesis is that sorting grain results in improved Fusarium head blight resistance. Both of the used wheat derived line populations have genetic variation, and population 1 has more than population 17. They are significantly different from each other for fusarium damged kernel (FDK), deoxynivalenol (DON), and other FHB traits. Although both populations are suitable to be grown for bulks, population 1 seems better since it has more genetic variation as well as lower FDK and DON, and earlier heading date. Lines within each population were significantly different and some lines in each population had significantly lower FDK and DON after selection using an optical sorter. Some lines had significant reduction in both FDK and DON, and some others had either FDK or DON reduction. Lines of population 1 that had significant reduction, were more numerous than in population 17, and FDK and DON reduction were greater.

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