scholarly journals Wheat dwarf virus infectious clones allow to infect wheat and Triticum monococcum plants

2019 ◽  
Vol 55 (No. 2) ◽  
pp. 81-89 ◽  
Author(s):  
Pavel Cejnar ◽  
Ludmila Ohnoutková ◽  
Jan Ripl ◽  
Jiban Kumar Kundu
Keyword(s):  
Systemic Infection ◽  
Triticum Monococcum ◽  
Dwarf Virus ◽  
Gene Gun ◽  
Wheat Dwarf Virus ◽  
Inoculation Method ◽  
Infectious Clones ◽  
Bacterial Plasmid ◽  
Biolistic Inoculation ◽  
Das Elisa

We constructed Wheat dwarf virus (WDV) infectious clones in the bacterial plasmids pUC18 and pIPKb002 and tested their ability to inoculate plants using Bio-Rad Helios Gene Gun biolistic inoculation method and Agrobacterium tumefaciens agroinoculation method, and we then compared them with the natural inoculation method via viruliferous P. alienus. Infected plants were generated using both infectious clones, whereas the agroinoculation method was able to produce strong systemic infection in all three tested cultivars of wheat and Triticum monococcum, comparable to plants inoculated by viruliferous P. alienus. Infection was confirmed by DAS-ELISA, and WDV titres were quantified using qPCR. The levels of remaining bacterial plasmid DNA were also confirmed to be zero.

scholarly journals First Report of Wheat dwarf virus in Winter Wheat in Finland

Plant Disease ◽  
2005 ◽  
Vol 89 (8) ◽  
pp. 912-912 ◽  
Author(s):  
A. Lemmetty ◽  
E. Huusela-Veistola
Keyword(s):  
Winter Wheat ◽  
Triticum Aestivum L ◽  
Dwarf Virus ◽  
Enzyme Linked Immunosorbent Assay ◽  
Wheat Dwarf Virus ◽  
Mean Values ◽  
Extraction Buffer ◽  
Yellow Traps ◽  
Positive Threshold ◽  
Das Elisa

During June and July of 2004, several diseased plants in winter wheat (Triticum aestivum L.) were reported by agricultural advisers in the southern and southwestern coastal area of Finland. The plants showed extreme dwarfing, various yellowing symptoms, and reduced or no heading. The damage varied considerably. Yield loss estimates in direct-drilled winter wheat fields were approximately 20 to 40% and in worst cases as much as 100%. A few leafhoppers (Psammotettix alienus Dahlb.) were collected from the field with sweep nets and yellow traps. Roots and symptomatic leaves of winter wheat and the leafhoppers were first tested using a commercial polyclonal antibody (DSMZ, Braunschweig, Germany) specific for Wheat dwarf virus (WDV). For the leaf and root samples, routine double-antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA) procedures were used. Five leafhoppers per sample were homogenized with the extraction buffer provided. The homogenate was centrifuged and the extract was evaluated using DAS-ELISA (2). The highest absorbance values were obtained from leafhoppers suspected to be viruliferous. The mean values varied from 1.002 to 1.990 after incubation in the substrate for 2 h. The absorbance values of several leaf samples exceeded the virus-positive threshold but were lower than those of the viruliferous leafhoppers. The virus was not detected in roots. Low absorbance values of virus-positive plants were confirmed using polymerase chain reaction (PCR) with primers specific for WDV (1). Total DNA extracts (DNeasy Plant Mini Kit; Qiagen, Hilden, Germany) from symptomatic leaves were tested using puRe Taq Ready-To-Go PCR beads (Amersham Biosciences, Buckinghamshire, UK). The PCR amplicon was the expected size (1,201 bp). The high absorbance value of the leafhoppers showed that the leafhoppers were carriers of the virus. These results confirmed that the causal agent of dwarfing and yellowing symptoms in winter wheat was WDV (genus Mastrevirus, family Geminiviridae). References: (1) A. Kvarnheden et al. Arch Virol 147:205, 2002. (2) J. Vacke and R. Cibulka. Plant Prot. Sci. 35:41, 2000.

scholarly journals Reaction of selected winter wheat varieties to autumnal infection with Wheat dwarf virus

2010 ◽  
Vol 41 (No. 1) ◽  
pp. 1-7 ◽  
Author(s):  
L. Širlová ◽  
J. Vacke ◽  
M. Chaloupková
Keyword(s):  
Winter Wheat ◽  
Grain Yield ◽  
Dwarf Virus ◽  
Yield Reduction ◽  
Wheat Dwarf Virus ◽  
Above Ground Biomass ◽  
Wheat Varieties ◽  
Small Plot ◽  
Das Elisa ◽  
Winter Wheat Varieties

The response of 25 registered winter wheat varieties to autumnal infection with Wheat dwarf virus (WDV) was studied in small plot trials in two years. The materials were infected by vectors, leafhopper Psammotettix alienus Dahlbom, 1851 from three-leaf stage to tillering. The symptoms expression was monitored in spring and plant height, weight of above ground biomass and grain yield were observed in summer. All tested varieties were evaluated as susceptible and divided into three groups: varieties Banquet and Svitava with 87.3–93.1% grain yield reduction as moderately susceptible, varieties Clever, Drifter, Niagara and Rialto with 95.6–97.68% grain yield reduction as susceptible and varieties Apache, Batis, Bill, Complet, Contra, Corsaire, Ludwig, Mladka, Nela, Record, Rheia, Semper, Sepstra, Solara, Sulamit, Tower, Trend, Vlasta and Winsdor with 99.7–100% grain yield reduction as very susceptible. Statistically significant differences were observed between moderately susceptible and susceptible varieties as well as very susceptible ones in absorbency values by means of DAS-ELISA.

Expression of engineered wheat dwarf virus in seed-derived embryos

1990 ◽  
Vol 79 (1) ◽  
pp. 158-162
Author(s):  
R. Topfer ◽  
B. Gronenborn ◽  
S. Schaefer ◽  
J. Schell ◽  
H.-H. Steinbiss
Keyword(s):  
Dwarf Virus ◽  
Wheat Dwarf Virus

scholarly journals First Report of Raspberry bushy dwarf virus Infecting Grapevine in Hungary

Plant Disease ◽  
2012 ◽  
Vol 96 (10) ◽  
pp. 1582-1582 ◽  
Author(s):  
I. Mavrič Pleško ◽  
M. Viršček Marn ◽  
K. Nyerges ◽  
J. Lázár
Keyword(s):  
Natural Infection ◽  
Dwarf Virus ◽  
Molecular Characteristics ◽  
Vitis Vinifera L ◽  
Southwestern Part ◽  
First Report ◽  
Raspberry Bushy Dwarf Virus ◽  
Two Samples ◽  
Rubus Species ◽  
Das Elisa

Raspberry bushy dwarf virus (RBDV) is the sole member of genus Idaeovirus and naturally infects Rubus species worldwide. It can be experimentally transmitted to many dicotyledonous plant species from different families. In Slovenia it has been reported to naturally infect grapevine, the first known non-Rubus natural host (3). However, RBDV from red raspberry and grapevine were found to be different in biological, serological, and molecular characteristics (4). From 2007 to 2010, grapevine (Vitis vinifera L.) vineyards were sampled in different parts of Hungary and tested for RBDV infection by double antibody sandwich (DAS)-ELISA using commercial reagents (Bioreba, Reinach, Switzerland). Overall, 181 samples were collected from 10 vineyards around Csörnyeföld, Badacsony, Eger, Tolcsva (Orémus), and Nagyréde. Samples were taken randomly unless plants showing virus-like symptoms were present, which were preferentially included in the survey. Two samples collected in 2010, each consisting of five leaves from five individual plants, tested positive by DAS-ELISA. They originated from a small private vineyard of Italian Riesling, Pinot Gris, and Rhein Riesling in the southwestern part of Hungary near Csörnyeföld where 29 samples were collected. All leaves were asymptomatic. Total RNA was extracted from positive samples using a RNeasy Plant Mini Kit (Qiagen, Hilden, Germany). cDNA was synthesized using primer RNA12 as described (4) and further amplified by PCR using primers RBDVUP1/RBDVLO4 that amplified an 872-bp fragment of RBDV coat protein and 3′ non-translated region (2). Amplification products from both samples were directly sequenced (Macrogen, Seoul, Korea). The sequences showed 98.6% identity between each other and were deposited in GenBank (Accession Nos. JQ928628 and JQ928629). Sequences were also compared with RBDV sequences deposited in GenBank. They showed 97.7 to 99.3% identity with RBDV sequences from grapevine from Slovenia and 94.2 to 96.1% with RBDV sequences from Rubus sp. Natural infection of grapevine with RBDV was first reported from Slovenia in 2003 (3) and was recently reported also from Serbia (1). To our knowledge, this is the first report of RBDV infection of grapevine in Hungary and suggests a wider presence of the virus in the region. References: (1) D. Jevremovic and S. Paunovic. Pestic. Phytomed. (Belgrade) 26:55, 2011. (2) H. I. Kokko et al. BioTechniques 20:842, 1996. (3) I. Mavric Pleško et al. Plant Dis. 87:1148, 2003. (4) I. Mavric Pleško et al. Eur. J. Plant Pathol. 123:261, 2009.

Analysis of complete genomes of isolates of the Wheat dwarf virus from new geographical locations and descriptions of their defective forms

Virus Genes ◽  
2013 ◽  
Vol 48 (1) ◽  
pp. 133-139 ◽  
Author(s):  
Jörg Schubert ◽  
Antje Habekuß ◽  
Beilei Wu ◽  
Thomas Thieme ◽  
Xifeng Wang
Keyword(s):  
Dwarf Virus ◽  
Wheat Dwarf Virus ◽  
Complete Genomes ◽  
Geographical Locations

Wheat dwarf virus. [Distribution map].

2018 ◽  
Author(s):  
Keyword(s):  
Triticum Aestivum ◽  
Czech Republic ◽  
Hordeum Vulgare ◽  
Avena Sativa ◽  
Dwarf Virus ◽  
Wheat Dwarf Virus ◽  
Distribution Map ◽  
Virus Distribution ◽  
New Distribution ◽  
Distribution In Europe

Abstract A new distribution map is provided for Wheat dwarf virus. Geminiviridae: Mastrevirus. Hosts: wheat (Triticum aestivum), barley (Hordeum vulgare), oat (Avena sativa). Information is given on the geographical distribution in Europe (Austria, Bulgaria, Czech Republic, Finland, France, Germany, Hungary, Italy, Poland, Romania, Slovakia, Slovenia, Spain, Sweden, UK, Ukraine), Asia (China, Hebei, Qinghai, Shaanxi, Shanxi, Sichuan, Yunnan, Iran, Syria, Turkey), Africa (Tunisia Zambia).

scholarly journals Tomato Twisted Leaf Virus: A Novel Indigenous New World Monopartite Begomovirus Infecting Tomato in Venezuela

Viruses ◽  
2019 ◽  
Vol 11 (4) ◽  
pp. 327 ◽  
Author(s):  
Gustavo Romay ◽  
Francis Geraud-Pouey ◽  
Dorys Chirinos ◽  
Mathieu Mahillon ◽  
Annika Gillis ◽  
...  
Keyword(s):  
Crop Production ◽  
New World ◽  
Systemic Infection ◽  
Plant Viruses ◽  
Monopartite Begomoviruses ◽  
Bipartite Begomoviruses ◽  
Reading Frame ◽  
Reverse Transcription Pcr ◽  
Infectious Clones ◽  
Leaf Virus

Begomoviruses are one of the major groups of plant viruses with an important economic impact on crop production in tropical and subtropical regions. The global spread of its polyphagous vector, the whitefly Bemisia tabaci, has contributed to the emergence and diversification of species within this genus. In this study, we found a putative novel begomovirus infecting tomato plants in Venezuela without a cognate DNA-B component. This begomovirus was genetically characterized and compared with related species. Furthermore, its infectivity was demonstrated by agroinoculation of infectious clones in tomato (Solanum lycopersicum) and Nicotiana benthamiana plants. The name Tomato twisted leaf virus (ToTLV) is proposed. ToTLV showed the typical genome organization of the DNA-A component of New World bipartite begomoviruses. However, the single DNA component of ToTLV was able to develop systemic infection in tomato and N. benthamiana plants, suggesting a monopartite nature of its genome. Interestingly, an additional open reading frame ORF was observed in ToTLV encompassing the intergenic region and the coat protein gene, which is not present in other closely related begomoviruses. A putative transcript from this region was amplified by strand-specific reverse transcription-PCR. Along with recent studies, our results showed that the diversity of monopartite begomoviruses from the New World is greater than previously thought.

scholarly journals Transmission of Chickpea chlorotic dwarf virus in Chickpea by the leafhopper Orosius albicinctus (Distant) in Pakistan – Short communication

2011 ◽  
Vol 47 (No. 1) ◽  
pp. 1-4 ◽  
Author(s):  
K.P. Akhtar ◽  
M. Ahmad ◽  
T.M. Shah ◽  
B.M. Atta
Keyword(s):  
Myzus Persicae ◽  
Short Communication ◽  
Polyclonal Antibodies ◽  
Viral Disease ◽  
Elisa Test ◽  
Dwarf Virus ◽  
Aphis Craccivora Koch ◽  
Orosius Albicinctus ◽  
Transmission Studies ◽  
Das Elisa

Chickpea chlorotic dwarf virus (CpCDV, genus Mastervirus, family Geminiviridae) is the most common viral disease of chickpea in Pakistan. Two aphid [Aphis craccivora Koch, Myzus persicae (Sulzer)], two leafhopper [Empoasca devastans Distant, Orosius albicinctus (Distant)] species and an unidentified brown leafhopper were collected in a chickpea field by hand and sweep nets for transmission studies of CpCDV. Transmission results showed that only the leafhopper O. albicinctus successfully transmitted the CpCDV from diseased to healthy chickpea plants. The presence of CpCDV in inoculated plants and the vector O. albicinctus were confirmed by DAS-ELISA test using specific polyclonal antibodies.

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