scholarly journals Molecular diversity and assessment of reactions of pepper pure line germplasm to Botrytis cinerea

2018 ◽  
Vol 54 (No. 3) ◽  
pp. 147-152
Author(s):  
Polat Ilknur ◽  
Baysal Ömür ◽  
Gümrükcü Emine ◽  
Sülü Görkem ◽  
Kitapci Aytül ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Molecular Diversity ◽  
Pure Line ◽  
Inter Simple Sequence Repeat ◽  
Resistance Level ◽  
Significant Information ◽  
Breeding Programs ◽  
Pure Lines ◽  
Simple Sequence ◽  
Issr Primers

The host resistance level of pure line materials was assessed in the genepool for the purpose of breeding. The highest resistance to the pathogen was observed in bell-type pepper. Moreover, genetic diversity of pure lines was investigated using selected inter-simple sequence repeat (ISSR) primers. Generally, genetic markers showed genetic diversity, so that long-type pure lines were separated from the other accessions. This is the first report on host reactions of Turkish pure lines as breeding material. These results provide significant information for future pepper breeding programs.

Genetic diversity in wild Lens spp. using inter simple sequence repeat (ISSR) marker

2015 ◽  
Vol 38 (5) ◽  
Author(s):  
Padmavati G. Gore ◽  
M. K. Rana ◽  
Kuldeep Tripathi ◽  
Mohar Singh ◽  
I. S. Bisht ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Simple Sequence Repeat ◽  
Molecular Diversity ◽  
Inter Simple Sequence Repeat ◽  
Issr Markers ◽  
Sequence Repeat ◽  
Principal Coordinates ◽  
Cultivated Species ◽  
Simple Sequence ◽  
Issr Primers

Genetic diversity was assessed in 50 accessions of seven <italic>Lens</italic> species using ISSR markers. The collection included accessions of the cultivated species <italic>L. culinaris</italic> and six wild species, <italic>viz</italic>., <italic>L. culinaris</italic> ssp. <italic>odemensis, L. culinaris</italic> ssp. <italic>orientalis</italic>, <italic>L.</italic> <italic>orientalis, L. nigricans, L. lamottei</italic> and <italic>L. ervoides.</italic> The 23 ISSR primers amplified a total of 368 bands with an average of 16 bands per primer. Maximum number of 20 bands was amplified using each of the primers ISSR-34 and ISSR-835. All the primers were found to be polymorphic. PIC values ranged from 0.02 to 0.80. The primers ISSR-807, ISSR- 809, ISSR- 827, ISSR- 847, ISSR-28 and ISSR- 37 were found to be very useful for analyzing the molecular diversity of the genus <italic>Lens</italic>. Cluster Analysis and Principal Coordinates Analyses placed the 50 accessions into two groups and complemented each other.

scholarly journals Genetic Diversity Analysis of Sugarcane Parents in Chinese Breeding Programmes Using gSSR Markers

2013 ◽  
Vol 2013 ◽  
pp. 1-11 ◽  
Author(s):  
Qian You ◽  
Liping Xu ◽  
Yifeng Zheng ◽  
Youxiong Que
Keyword(s):  
Genetic Diversity ◽  
Molecular Diversity ◽  
Genetic Relationships ◽  
Breeding Programs ◽  
Sugarcane Varieties ◽  
Breeding Station ◽  
The World ◽  
Breeding Programmes ◽  
Genetic Structures ◽  
Simple Sequence

Sugarcane is the most important sugar and bioenergy crop in the world. The selection and combination of parents for crossing rely on an understanding of their genetic structures and molecular diversity. In the present study, 115 sugarcane genotypes used for parental crossing were genotyped based on five genomic simple sequence repeat marker (gSSR) loci and 88 polymorphic alleles of loci (100%) as detected by capillary electrophoresis. The values of genetic diversity parameters across the populations indicate that the genetic variation intrapopulation (90.5%) was much larger than that of interpopulation (9.5%). Cluster analysis revealed that there were three groups termed as groups I, II, and III within the 115 genotypes. The genotypes released by each breeding programme showed closer genetic relationships, except the YC series released by Hainan sugarcane breeding station. Using principle component analysis (PCA), the first and second principal components accounted for a cumulative 76% of the total variances, in which 43% were for common parents and 33% were for new parents, respectively. The knowledge obtained in this study should be useful to future breeding programs for increasing genetic diversity of sugarcane varieties and cultivars to meet the demand of sugarcane cultivation for sugar and bioenergy use.

scholarly journals Genetic Diversity Assessment of Selected Annona muricata L. Germplasm in Sri Lanka

2020 ◽  
pp. 1-13
Author(s):  
S. H. M. R. N. P. Samaradiwakara ◽  
W. L. G. Samarasinghe ◽  
P. G. S. Shantha ◽  
K. G. C. N. Jayarathna ◽  
P. Dehigaspitiya ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Sri Lanka ◽  
Simple Sequence Repeat ◽  
Inter Simple Sequence Repeat ◽  
Issr Markers ◽  
Polymorphic Band ◽  
Annona Muricata ◽  
Sequence Repeat ◽  
Breeding Programs ◽  
Simple Sequence

Annona muricata L. commonly known as soursop is an underutilized fruit crop species in Sri Lanka gaining much importance in the recent past due to its high nutritional and medicinal value. Soursop germplasm collections are available within the country and assessing the genetic diversity is needed to proceed with conservation, detecting promising lines and breeding programs. This study was conducted to assess the genetic diversity of 50 soursop individuals using Inter-Simple Sequence Repeat (ISSR) markers. The study was conducted at Plant Genetic Resources Centre of the Department of Agriculture in Gannoruwa during 2017 to 2019. DNA of the 50 soursop samples were extracted using CTAB method and Polymerase Chain Reaction (PCR) was carried using 13 Inter Simple Sequence Repeat (ISSR) Markers. PCR products were visualized using 1.5 percent Agarose gel electrophoresis under the Biorad Gel documentation system and analyzed using POPGENE 1.31. PCR amplified 139 bands from 13 ISSR markers among which 118 were found to be polymorphic. The polymorphic band percentage was 85 percent while as the average number of bands observed (Na) was 1.8489 and the effective allele number (Ne) was 1.5377. The Nei's gene diversity index (h) was 0.3079. The Shannon Information Index (I) found to be 0.4556. Dendrogram constructed based on the UPGMA method clustered the studied accessions into four major clusters at 80 percent similarity level. Results revealed considerable degree of genetic diversity existed within the studied soursop germplasms at Sri Lanka. Existing genetic diversity within soursop individuals will serve as germplasm bank to identify and utilize potential germplasm resources for conservation and future breeding programs to develop quality soursop varieties in Sri Lanka.

scholarly journals Genetic diversity in apple tree cultivars established in the Sierra de Arteaga, Coahuila, Mexico

2018 ◽  
Vol 5 (13) ◽  
pp. 65
Author(s):  
Mayra Alejandra Escobar Saucedo ◽  
Cristobal Aguilar ◽  
Antonio Vázquez Ramos ◽  
Manuel Humberto Reyes Valdés ◽  
Raúl Rodríguez Herrera
Keyword(s):  
Genetic Diversity ◽  
Genetic Risk ◽  
Simple Sequence Repeat ◽  
Apple Tree ◽  
Inter Simple Sequence Repeat ◽  
Apple Cultivars ◽  
Apple Production ◽  
Issr Molecular Markers ◽  
Simple Sequence ◽  
Issr Primers

Apple production in the Sierra de Arteaga, Coahuila is mainly based on the Golden Delicious cultivar. Production based on only a few cultivars represents a genetic risk, because if they are susceptible to a pest, disease or climate change much of this production would be lost if one or more of these conditions occurred. Foreseeing this situation, farmers have introduced different commercial apple cultivars, but there is little information about the genetic diversity among them. Therefore, the aim of this study was to estimate the rates of intraspecic genetic diversity of 12 different apple cultivars using inter-simple sequence repeat (ISSR) molecular markers. Employing nine ISSR primers, it was possible to identify 124 DNA bands, of which 63% were polymorphic, with genetic diversity of 0.24. Results suggest that these cultivars are adapted to the region's climate and can be utilized to increase the overall plantation area, in order not to depend on a single cultivar.

scholarly journals Evaluation of genetic diversity of durum wheat (Triticum durum desf.) genotypes using inter-simple sequence repeat (ISSR) and caat box-derived polymorphism (CBDP) markers

Genetika ◽  
2020 ◽  
Vol 52 (3) ◽  
pp. 895-909
Author(s):  
Mahmood Aslan-Parviz ◽  
Mansoor Omidi ◽  
Varahram Rashidi ◽  
Alireza Etminan ◽  
Alireza Ahmadzadeh
Keyword(s):  
Genetic Diversity ◽  
Durum Wheat ◽  
Simple Sequence Repeat ◽  
Genetic Parameters ◽  
Inter Simple Sequence Repeat ◽  
Molecular Technique ◽  
Sequence Repeat ◽  
Wheat Genotypes ◽  
Simple Sequence ◽  
Issr Primers

Evaluation of genetic diversity is the key principal for plant breeding, providing an opportunity to discover novel characters and alleles for breeders. In the present study, 69 durum wheat genotypes were investigated for genetic diversity using several CAAT box-derived polymorphism (CBDP) and inter-simple sequence repeat (ISSR) markers. Twelve CBDP and sixteen ISSR primers amplified a total of 115 and 160 polymorphic fragments with a mean of 9.58 and 10 fragments per primer, respectively. CBDP primers showed the higher mean values for informativeness parameters such as polymorphic information content (PIC), resolving power (Rp) and marker index (MI) in comparison with ISSR primers. The results of analysis of molecular variance (AMOVA) indicated that the highest proportion of genetic variance referred within populations. Furthermore, CBDP primers indicated high values for all genetic parameters. Besides, the highest values of genetic parameters including number of observed (Na) and effective alleles (Ne), Shannon?s information index (I) and Nei?s gene diversity (He) were estimated for Iranian durum wheat landraces. Cluster analysis based on each molecular technique classified all durum wheat genotypes into three main groups, so that the results of principal coordinate analysis (PCoA) supported the grouping patterns. As a result, the grouping pattern observed by ISSR primers was clearer than CBDP primers and grouped all samples based on their origins. However, Mantel?s coefficient correlation test illustrated the higher positive correlation (0.54) between both marker techniques. Hence, the use of these markers in combination with each other to evaluate the genetic diversity is recommended.

Preliminary Assessment of Genetic Diversity in Cultivated Glycyrrhiza uralensis, G. inflate and G. glabra by Chemical Fingerprint and Inter-Simple Sequence Repeat Markers

2011 ◽  
Vol 347-353 ◽  
pp. 1318-1325 ◽  
Author(s):  
Ting Liu ◽  
Hai Ming Lin
Keyword(s):  
Genetic Diversity ◽  
Simple Sequence Repeat ◽  
Inter Simple Sequence Repeat ◽  
Issr Markers ◽  
Glycyrrhiza Uralensis ◽  
Sequence Repeat ◽  
Chemical Fingerprint ◽  
Chemical Fingerprints ◽  
Simple Sequence ◽  
Issr Primers

The two main secondary metabolites in Glycyrrhiza Species are Glycyrrhizic acid and liquiritin. They are considered as active ingredients . The content of these compounds showed variation in different species. Standard chemical fingerprints were generated from cultivated Glycyrrhiza uralensis, G. inflate and G. glabra, which could be identification markers. Five efficient inter-simple sequence repeat (ISSR) primers were screened and optimized for detecting the genetic diversity in three cultivated Glycyrrhiza uralensis, G. inflate and G. glabra. By using two characteristic peaks compare with three cultivars, Glycyrrhiza uralensis and G. glabra were bigger similarity than G. inflate. The results is in accordance with the results by ISSR markers. The higher genetic diversity in G. inflate was useful to more broad breeding. Our result suggest that provides an optimized method for assessment genetic diversity of cultivated Glycyrrhiza uralensis, G. inflate and G. glabra using Chemical fingerprint and ISSR markers which is useful for further investigation in breeding.

Genetic diversity of Symplocos paniculata of Hunan province revealed by inter-simple sequence repeat (ISSR)

2019 ◽  
Vol 51 (5) ◽  
Author(s):  
Huifang Cao ◽  
Qiang Lin ◽  
Peiwang Li ◽  
Jingzhen Chen ◽  
Changzhu Li ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Simple Sequence Repeat ◽  
Inter Simple Sequence Repeat ◽  
Hunan Province ◽  
Sequence Repeat ◽  
Simple Sequence

ISSR Marker Based Genetic Diversity in Morinda Spp. For Its Enhanced Collection, Conservation and Utilization

2021 ◽  
Author(s):  
Lalit Arya ◽  
Ramya Kossery Narayanan ◽  
Anjali Kak ◽  
Chitra Devi Pandey ◽  
Manjusha Verma ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Gene Diversity ◽  
Inter Simple Sequence Repeat ◽  
Issr Markers ◽  
Morinda Citrifolia ◽  
Species Differentiation ◽  
Information Index ◽  
Upgma Cluster Analysis ◽  
Simple Sequence

Abstract Morinda (Rubiaceae) is considerably recognized for its multiple uses viz. food, medicine, dyes, firewood, tools, oil, bio-sorbent etc. The molecular characterization of such an important plant would be very useful for its multifarious enhanced utilization. In the present study, 31 Morinda genotypes belonging to two different species Morinda citrifolia and Morinda tomentosa collected from different regions of India were investigated using Inter Simple Sequence Repeat (ISSR) markers. Fifteen ISSR primers generated 176 bands with an average of 11.7 bands per primer, of which (90.34%) were polymorphic. The percentage of polymorphic bands, mean Nei’s gene diversity, mean Shannon’s information index in Morinda tomentosa and Morinda citrifolia was [(69.89%, 30.68%); (0.21 ± 0.19, 0.12 ± 0.20); (0.32 ± 0.27 0.17 ± 0.28)] respectively, revealing higher polymorphism and genetic diversity in Morinda tomentosa compared to Morinda citrifolia. Structure, and UPGMA cluster analysis placed the genotypes into well-defined separate clusters belonging to two species Morinda tomentosa and Morinda citrifolia revealing the utility of ISSR markers in species differentiation. Distinct ecotypes within a particular species could also be inferred emphasizing the collection and conservation of Morinda genotypes from different regions, in order to capture the overall diversity of respective species. Further higher diversity of M. tomentosa must be advanced for its utilization in nutraceutical, nutritional and other nonfood purposes.

Sign in / Sign up

Export Citation Format

Share Document