scholarly journals Gene flow was not detected from a field trial of transgenic plum cv. HoneySweet – Short Communication

2019 ◽  
Vol 55 (No. 2) ◽  
pp. 90-92
Author(s):  
Petr Komínek ◽  
Jaroslav Polák ◽  
Marcela Komínková ◽  
Ralph Scorza
Keyword(s):  
Czech Republic ◽  
Gene Flow ◽  
Field Test ◽  
Field Trial ◽  
Short Communication ◽  
Test Site ◽  
Plum Pox Virus ◽  
Experimental Plot ◽  
The Czech Republic ◽  
Crop Research

A field trial with plum cv. HoneySweet was established in 2003 in an experimental plot near Crop Research Institute in Prague-Ruzyně, Czech Republic, on the basis of a permission issued by the Ministry of the Environment of the Czech Republic. In addition to the evaluation of resistance of plum cv. HoneySweet to Plum pox virus, the field trial was used to evaluate gene flow of the inserted transgene. Sampling of blackthorn and myrobalan trees outside the field test site occurred at distances ranging from 544 m to 845 m from the test site and showed no gene flow, testing both plants and seeds collected from blackthorns and myrobalans. Similarly, seeds from plums cv. Jojo growing directly at the field test place did not show any presence of the transgene after seven years of evaluation. 

scholarly journals Reaction of transgenic plum cv. HoneySweet to the Plum pox virus after a severe infection of Monilinia sp. – short communication

2018 ◽  
Vol 55 (No. 1) ◽  
pp. 8-10 ◽  
Author(s):  
Polák Jaroslav ◽  
Neubauerová Tereza ◽  
Komínek Petr ◽  
Kundu Jiban Kumar
Keyword(s):  
Czech Republic ◽  
Short Communication ◽  
Severe Infection ◽  
Pcr Detection ◽  
Plum Pox Virus ◽  
Prunus Domestica ◽  
Rt Pcr ◽  
The Czech Republic ◽  
Das Elisa ◽  
Year 2013

Resistance to Plum pox virus (PPV) in transgenic Prunus domestica L., clone C5 (cv. HoneySweet) was evaluated in a regulated field in the Czech Republic for fifteen years (2002–2016). PPV mild symptoms appeared in C5 trees only in several leaves situated close to the point of inoculum grafting up to 2010. No symptoms of PPV were observed in the years 2011–2013 and results of ELISA and RT-PCR detection tests were negative. In the twelfth year (2013), there was a severe unusual natural attack of plum trees by Monilinia sp. This Monilinia sp. attack occurred only one time – in 2013. There was no Monilinia sp. infection in 2002–2012 and in 2014–2016. Mild PPV symptoms reappeared in several leaves of transgenic plum trees in the next two years (2014–2015) and the presence of PPV was proved by DAS-ELISA and confirmed by RT-PCR.

scholarly journals Cherry virus A infecting cherries and plums in the Czech Republic – Short communication

2013 ◽  
Vol 40 (No. 1) ◽  
pp. 37-39 ◽  
Author(s):  
D. Šafářová ◽  
M. Navrátil ◽  
F. Paprštein ◽  
T. Candresse ◽  
A. Marais
Keyword(s):  
Phylogenetic Analysis ◽  
Czech Republic ◽  
Sweet Cherry ◽  
Short Communication ◽  
Rt Pcr ◽  
Pcr Assay ◽  
The Czech Republic ◽  
Germplasm Collections ◽  
Sweet Cherries

 The presence of Cherry virus A (CVA) in the germplasm collections of sweet cherries and plums was studied. CVA was detected using the specific RT-PCR assay in six of eight sweet cherry and one of four plum cultivars. Specifity of amplicons and distant position of cherry and non-cherry isolates was verified by sequencing and phylogenetic analysis. Results indicate that the cherry landraces and cultivars could be infected by CVA more than it has been assumed.

scholarly journals Occurrence of rust disease caused by Puccinia oxalidis on Oxalis triangularis in the Czech Republic – Short Communication 

2014 ◽  
Vol 50 (No. 1) ◽  
pp. 17-18
Author(s):  
I. Šafránková
Keyword(s):  
Czech Republic ◽  
Leaf Spot ◽  
Short Communication ◽  
Rust Disease ◽  
The Czech Republic ◽  
First Report ◽  
Microscopic Features

This is the first report of Puccinia oxalidis causing leaf spot diseases on ornamental Oxalis triangularis subsp. papilionaceae cv. Atropurpurea in Moravia, Czech Republic. The macroscopic symptoms and microscopic features are described.

scholarly journals Occurrence of viruses on pepper plantations in the Czech Republic – Short communication

2012 ◽  
Vol 39 (No. 3) ◽  
pp. 139-143 ◽  
Author(s):  
J. Svoboda ◽  
L. Svobodová-Leišová
Keyword(s):  
Czech Republic ◽  
Mosaic Virus ◽  
Short Communication ◽  
Alfalfa Mosaic Virus ◽  
Sources Of Resistance ◽  
Pepper Mild Mottle Virus ◽  
The Czech Republic ◽  
Tomato Spotted Wilt ◽  
Wilt Virus ◽  
Broad Bean Wilt Virus

A survey of viruses on capsicum plants in the Czech Republic was carried out in the years 2006–2010. Altogether, 375 leaf samples with symptoms suggesting viral infection were collected both from open fields and greenhouses. These samples were examined for the presence of Alfalfa mosaic virus (AMV), Broad bean wilt virus-1 (BBWV-1), Cucumber mosaic virus (CMV), Pepper mild mottle virus (PMMoV), Potato virus Y (PVY), Tobacco mosaic virus (TMV) and Tomato spotted wilt virus (TSWV) by ELISA. Viruses detected in the samples were AMV, BBWV-1, CMV and PVY. The most prevalent were CMV and PVY which were present in 24 and 29% of tested samples, respectively. In some cases a complex infection of two viruses was detected. Gene sources of resistance against CMV and PVY are mentioned. The relation of virus occurrence on aphid incidence is discussed.

scholarly journals Influence of climate changes in the Czech Republic on the distribution of plant viruses and phytoplasmas originally from the mediterranean subtropical region

2010 ◽  
Vol 45 (Special Issue) ◽  
pp. S20-S26 ◽  
Author(s):  
J. Polák
Keyword(s):  
Czech Republic ◽  
Climate Changes ◽  
Plant Pathogens ◽  
Zucchini Yellow Mosaic Virus ◽  
Plant Viruses ◽  
Northern Italy ◽  
Yellow Mosaic Virus ◽  
Plum Pox Virus ◽  
The Czech Republic ◽  
Peach Trees

Results of research aiming at monitoring of climate changes impact on plant pathogens distribution such as <i>Zucchini yellow mosaic virus</i> (ZYMV), quarantine <i>Plum pox virus</i> (PPV) and quarantine phytoplasma European stone fruit yellows (ESFY) are presented here. ZYMV has spread from Northern Italy across Austria up to Central Moravia and Bohemia. PPV has been continuously spreading from the lowlands of Central Bohemia and Moravia up to plains. Later, from the sixties and seventies of the last century, due to climate warming and human activities the virus quickly spread to uplands, foothills and mountains of the Czech Republic. Phytoplasma ESFY was spreading in a manner similar to ZYMV in the eighties of the twentieth century from Northern Italy and currently is affecting mainly apricot and peach trees in Southern Moravia.

scholarly journals First report of Pepper mild mottle virus in pepper seeds produced in the Czech Republic – Short Communication

2010 ◽  
Vol 42 (No. 1) ◽  
pp. 34-37 ◽  
Author(s):  
J. Svoboda ◽  
G. Červená ◽  
J. Rodová ◽  
M. Jokeš
Keyword(s):  
Czech Republic ◽  
Electron Microscope ◽  
Resistance Genes ◽  
Short Communication ◽  
Mottle Virus ◽  
Pepper Mild Mottle Virus ◽  
The Czech Republic ◽  
First Report ◽  
Viral Particles ◽  
Straight Rod

Symptoms of viral infection were observed on plants of pepper, cv. OL 228, raised from commercial seeds of Czech origin in a greenhouse in the year 2002. Infected plants showed mosaic or mottling on leaves, and necrotic depressions on fruits. Straight, rod-shaped viral particles of about 300 nm, indicating a tobamovirus infection, were found by electron microscope. ELISA produced negative reactions for <i>Tobacco mosaic virus</i> (TMV) but positive reactions with an antiserum to <i>Pepper mild mottle virus</i> (PMMoV). In biological characterisation using pepper cultivars with the L1, L2, L3 and L4 tobamovirus resistance genes it was found that the Czech isolate of PMMoV belongs to pathotype P1.2. This is the first report of PMMoV in the Czech Republic. Its distribution, however, may still be limited as a survey did not reveal other infections in the main pepper producing areas. As PMMoV spreads with infected seeds, the possibility of its chemical deactivation by NaOH was tested and confirmed.

scholarly journals Goji berry gall mite Aceria kuko occurrence in the Czech Republic – short communication

2017 ◽  
Vol 54 (No. 1) ◽  
pp. 39-42 ◽  
Author(s):  
Hrudová Eva ◽  
Šafránková Ivana
Keyword(s):  
European Union ◽  
Czech Republic ◽  
Short Communication ◽  
Lycium Chinense ◽  
The European Union ◽  
Asian Origin ◽  
The Czech Republic ◽  
Goji Berry ◽  
Gall Mite ◽  
Lycium Chinense Miller

Damage caused by the Goji berry gall mite, Aceria kuko (Kishida, 1927) was observed on the leaves of Lycium chinense Miller (Solanaceae) in Brno in August 2016 and on L. barbarum L. in Popovice in September 2016. Aceria kuko is a pest of Asian origin which is common in China and occurs only sporadically on Goji berry plants in the European Union. This is the first observation of the Goji berry gall mite occurrence in the Czech Republic.

Short communication: Molecular analysis of Potato leafroll virus isolates from the Czech Republic

Virus Genes ◽  
2009 ◽  
Vol 39 (1) ◽  
pp. 153-155 ◽  
Author(s):  
Helena Plchova ◽  
Noemi Cerovska ◽  
Tomas Moravec ◽  
Petr Dedic
Keyword(s):  
Czech Republic ◽  
Molecular Analysis ◽  
Short Communication ◽  
Potato Leafroll Virus ◽  
The Czech Republic ◽  
Leafroll Virus ◽  
Virus Isolates

scholarly journals Outdoor occurrence of stored-product pests (Coleoptera) in the vicinity of a grain store – Short communication

2010 ◽  
Vol 41 (No. 2) ◽  
pp. 86-89 ◽  
Author(s):  
Z. Kučerová ◽  
R. Aulický ◽  
V. Stejskal
Keyword(s):  
Czech Republic ◽  
Species Composition ◽  
Short Communication ◽  
Insect Pests ◽  
Sitophilus Granarius ◽  
The Czech Republic ◽  
Pest Monitoring ◽  
Stored Product Pests ◽  
Bait Traps ◽  
First Time

The species composition, frequency and abundance of insect pests (Coleoptera) occurring outside a grain store were explored. Wheat bait traps were used for pest monitoring; they were changed every month from April to September. For the first time the outdoor occurrence of primary and secondary stored-product pests was documented in the Czech Republic. Seven species of Coleoptera were found outside the grain store. Pest abundance and frequency were in high correlation. The primary pest Sitophilus granarius was dominant.

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