scholarly journals Histological changes of the sciatic nerve in dogs after intraneural application of lidocaine--relation to the established application pressure

2005 ◽  
Vol 5 (1) ◽  
pp. 8-13 ◽  
Author(s):  
Zakira Mornjaković ◽  
Faruk Dilberović ◽  
Esad Ćosović ◽  
Kučuk-Alija Divanović ◽  
Asja Začiragić ◽  
...  
Keyword(s):  
Individual Differences ◽  
Sciatic Nerve ◽  
Inflammatory Process ◽  
Structural Changes ◽  
Proximal Part ◽  
Serial Sections ◽  
Single Application ◽  
Histological Changes ◽  
Average Value ◽  
Covering System

Histological changes of sciatic nerve in adult dogs 7 days after single application of 2% lido-caine (4 ml dose, speed of injection 3 ml/min) and measurement of the application pressure was studied, with a goal to investigate structural changes of the nerve in relation to the established pressure values. The application pressure was determined by using Bio Bench software. In intrafascicular puncture an average application pressure of 198.23 ± 52 kPa was found, and in interfascicular puncture its average value was 53.3 ± 17.9 kPa, with a note that individual differences are regularly present. Seven days after the injection, a nerve dissection was performed and serial sections covering the region of injection’s puncture and bordering proximal and distal zones, in the total length of 3 cm, were prepared. The found changed show the presence of nerves’ fibers lesions with a strong reactivity of Schwann’s cell, as well as the change of interstitial structure concerning hypercellularity and occurrence of cellular extravasation. The covering system of the nerve in the zone of epineurium manifests changes of inflammatory process and in perineurium a decomposition of lamella layers and the alteration of their tinctorial properties were noticed. A comparison of the found nerve reactivities in intra- and interfascicular application showed their one-way alteration, although the lesions were more noticeable in the conditions of intrafascicular application. The damages were mostly expressed in the zone of local application of anesthetic, than distally from it, while the damage to the structure in the proximal part is of the smallest degree.

Application of stereological analysis of cell volume to isolated myocytes in culture with and without adrenergic innervation

1990 ◽  
Vol 48 (3) ◽  
pp. 416-417
Author(s):  
Jane K. Rosenthal ◽  
Dianne L. Atkins ◽  
William J. Marvin ◽  
Penny A. Krumm
Keyword(s):  
Cardiac Myocytes ◽  
Structural Changes ◽  
Three Dimensional ◽  
Adrenergic Innervation ◽  
Culture Cell ◽  
Serial Sections ◽  
Newborn Rats ◽  
Primary Culture Cell ◽  
Biological Studies ◽  
Cell Volumes

To comprehend structural changes in cardiac myocytes accompanying adrenergic innervation, it is essential that a three dimensional analysis be performed. To date, biological studies which utilize stereological methods have been limited to cells in tissue and in organs. Our laboratory has utilized current stereological techniques for measuring absolute volumes of individual myocytes in primary culture. Cell volumes are calculated for two distinct groups of cells at 96 hours in culture: isolated myocytes and myocytes innervated with adrenergic neurons (Figure 1).Cardiac myocytes are cultured from the ventricular apices of newborn rats. Cells are plated directly onto tissue culture dishes with or without preplated explants from the paravertebral thoracolumbar sympathetic chain. On day four cultures are photographed and marked for one-to-one cell location. Following conventional fixation and embeddment in eponate-12, the cells are relocated and mounted for microtomy. The cells are completely sectioned at 120nm in their parallel orientation to the surface of the dish (Figure 2). Serial sections are collected on formvar coated slotted grids and are recorded in sequence.

HISTOLOGICAL CHANGES IN THE INTESTINES OF POULTRY DURING FODDER INTOXICATION

2020 ◽  
Author(s):  
E.P. Dolgov ◽  
◽  
A.A. Abramov ◽  
E.V. Kuzminova ◽  
E.V. Rogaleva ◽  
...  
Keyword(s):  
Body Weight ◽  
Histological Examination ◽  
Aflatoxin B1 ◽  
Structural Changes ◽  
Clinical Manifestations ◽  
Feed Additives ◽  
The Body ◽  
Histological Changes ◽  
Beet Pulp

The article presents the data on the study of the influence of mycotoxins combination (T-2 toxin at the concentration of 0.095 mg/kg and aflatoxin B1 in the concentration of 0.019 mg/kg) on the body of quails and the results of pharmacocorrection of toxicosis with a complex consisting of beet pulp and lecithin. Structural changes in the intestines of quais at fodder mycotoxicosis are described. The use of antitoxic feed additives in poultry led to a weakening of the action of xenobiotics, which was confirmed by an increase in the safety of poultry and increase in body weight of quails, a decrease in the clinical manifestations of intoxication, as well as in positive changes in the structure of the intestine of the poultry during histological examination.

Individual differences in white and grey matter structure associated with verbal habits of thought

2019 ◽  
Author(s):  
Justin C. Hayes ◽  
Katherine L Alfred ◽  
Rachel Pizzie ◽  
Joshua S. Cetron ◽  
David J. M. Kraemer
Keyword(s):  
Individual Differences ◽  
White Matter ◽  
Cognitive Processing ◽  
Grey Matter ◽  
Structural Changes ◽  
Structural Integrity ◽  
Diffusion Tensor ◽  
Self Report ◽  
White Matter Tracts

Modality specific encoding habits account for a significant portion of individual differences reflected in functional activation during cognitive processing. Yet, little is known about how these habits of thought influence long-term structural changes in the brain. Traditionally, habits of thought have been assessed using self-report questionnaires such as the visualizer-verbalizer questionnaire. Here, rather than relying on subjective reports, we measured habits of thought using a novel behavioral task assessing attentional biases toward picture and word stimuli. Hypothesizing that verbal habits of thought are reflected in the structural integrity of white matter tracts and cortical regions of interest, we used diffusion tensor imaging and volumetric analyses to assess this prediction. Using a whole-brain approach, we show that word bias is associated with increased volume in several bilateral language regions, in both white and grey matter parcels. Additionally, connectivity within white matter tracts within an a priori speech production network increased as a function of word bias. These results demonstrate long-term structural and morphological differences associated with verbal habits of thought.

Morphophysiological studies of the shoot apex. I. Recurrent growth flushes and their relationship with structural changes in the growing apex of Callistemon viminalis

1968 ◽  
Vol 46 (10) ◽  
pp. 1287-1295 ◽  
Author(s):  
A. N. Purohit ◽  
K. K. Nanda
Keyword(s):  
Structural Changes ◽  
Rest Period ◽  
Growth Cycle ◽  
Active Period ◽  
Active Growth ◽  
Histological Changes ◽  
Floral Buds ◽  
Rest Periods ◽  
Number Of Leaves ◽  
Callistemon Viminalis

The annual growth cycle of Callistemon viminalis consists of three phases: (1) a reproductive phase that continues till the end of February; (2) a vegetative phase that lasts from the end of February till the end of August; and (3) a long dormant phase which lasts till the end of December. The cycle exhibits four growth flushes with alternating periods of active growth and rest. The duration of the successive rest periods increases, leading eventually to the onset of the long dormant phase.The growing apex exhibits more or less similar histological changes in different growth flushes. An increase in divisional activity of the cells of flanking meristem, accompanied by a decline in the elongation of cells of pith rib meristem, characterizes the rest period. During the active period of growth, the cells of the pith rib meristem elongate but the activity of the flanking meristem decreases. The number of leaves produced in each growth flush, therefore, is dependent upon the duration of the preceding rest period, and their size and nature on the duration of active growth. In growth flush 1 the cells of the rib meristem show elongation twice during the active period corresponding with the separation of floral buds and of foliage leaves respectively. In the long dormant phase, on the other hand, the cells of the flanking meristem show high divisional activity twice, first during September when new leaf primordia are initiated, and again during November when floral buds are initiated. Events of great significance thus occur during the long dormant phase. The reproductive development is characterized by a marked increase in the rate of cell division in the flanking meristem accompanied by cessation of growth, the features that characterize the rest period as well.

scholarly journals Haematoxylon campechianum Extract Ameliorates Neuropathic Pain via Inhibition of NF-κB/TNF-α/NOX/iNOS Signalling Pathway in a Rat Model of Chronic Constriction Injury

Biomolecules ◽  
2020 ◽  
Vol 10 (3) ◽  
pp. 386 ◽  
Author(s):  
Mansour Sobeh ◽  
Mona F. Mahmoud ◽  
Samar Rezq ◽  
Mohamed A.O. Abdelfattah ◽  
Islam Mostafa ◽  
...  
Keyword(s):  
Neuropathic Pain ◽  
Sciatic Nerve ◽  
Brain Stem ◽  
Rat Model ◽  
Structural Changes ◽  
Chronic Constriction Injury ◽  
Biological Activities ◽  
Ethanol Extract ◽  
Phenolic Constituents ◽  
Anti Inflammatory

In this study, the phytochemical composition and the possible prophylactic effects of an aqueous ethanol extract of Haematoxylon campechianum flowers (HCF) on peripheral neuropathic pain in a chronic constriction injury (CCI) rat model are investigated. Rats with induced CCI were subjected to neuropathic pain behaviour tests and evaluated by chemical, thermal, and mechanical sensation tests and functional recovery of the brain stem and sciatic nerve at 7- and 14-day intervals. The effect of the extract on acute pain and inflammation is also investigated. The extract exerted both peripheral and central analgesic and anti-inflammatory properties in addition to antipyretic effects that are clear from targeting COX, LOX and PGE. It was found that CCI produced significant thermal and mechanical hyperalgesia, cold allodynia and deleterious structural changes in both sciatic nerve and brain stem. Treatments with HCF extract significantly improved cold and thermal withdrawal latency, mechanical sensibility and ameliorated deleterious changes of sciatic nerve and brain stem at different dose levels. The extract also ameliorated oxidative stress and inflammatory markers in brain stem and sciatic nerve. It suppressed the apoptotic marker, p53, and restored myelin sheath integrity. The effects of HCF extract were more potent than pregabalin. Fifteen secondary metabolites, mainly gallotannins and flavonoids, were characterized in the extract based on their retention times and MS/MS data. The identified phenolic constituents from the extract could be promising candidates to treat neuropathic pain due to their diverse biological activities, including antioxidant, anti-inflammatory and neuroprotective properties.

scholarly journals A PATHOLOGICAL STUDY OF SYPHILITIC AORTITIS AND ITS SEROLOGY

1916 ◽  
Vol 23 (1) ◽  
pp. 25-38 ◽  
Author(s):  
John H. Larkin ◽  
I. J. Levy
Keyword(s):  
Guinea Pig ◽  
Inflammatory Process ◽  
Alcoholic Extract ◽  
Round Cell ◽  
Pathological Study ◽  
Guinea Pig Heart ◽  
Histological Changes ◽  
Round Cell Infiltration ◽  
Constant Feature ◽  
Syphilitic Aortitis

1. Syphilitic aortitis is a productive inflammatory process, the earliest and most constant feature of which is a perivascular round cell infiltration in the adventitia. 2. The typical gross picture of luetic aortitis is often obscured by a superimposed, diffuse atherosclerosis. In the early cases the aorta appears fairly normal, presenting only the characteristic histological changes. 3. A pure aortic insufficient valve, with the exception of an infectious endocarditis, is always luetic. 4. Cardiac hypertrophy is not a complication of luetic aortitis. When present it is usually associated with a nephritis. 5. The demonstration of Spirochœta pallida, even in advanced specimens of syphilitic aortas, is doubtful. 6. An antigen prepared from alcoholic extract of guinea pig heart with the original Wassermann technique should be preferred in diagnosing luetic aortitis. 7. Positive complement fixations in patients suffering with syphilis for a period of about fifteen years or longer suggest the probability, at least, of histological luetic changes in the aorta in 80 to 90 per cent of the cases. 60 per cent of these die from aortitis. 8. About 94 per cent of patients suffering with aortitis give positive Wassermann reactions.

Applied Anatomy of the Paranasal Sinuses with Emphasis on Endoscopic Surgery

1994 ◽  
Vol 103 (4_suppl) ◽  
pp. 3-32 ◽  
Author(s):  
Stephen P. Becker
Keyword(s):  
Endoscopic Surgery ◽  
Paranasal Sinuses ◽  
Endoscopic Sinus Surgery ◽  
Structural Changes ◽  
Chronic Sinusitis ◽  
Sinus Surgery ◽  
Serial Sections ◽  
Anatomic Landmarks ◽  
Applied Anatomy

Serial sections of frozen cadaver heads were obtained in the axial, coronal, and sagittal planes. These were analyzed with specific attention to the anatomy of the paranasal sinuses as it pertains to endoscopic sinus surgery. Important anatomic landmarks are pointed out. The use of these landmarks to guide the surgeon through the dissection and to avoid complications is discussed. The resolution of chronic sinusitis is often brought about by the structural changes secondary to endoscopic sinus surgery, and the reasons for this outcome are reviewed.

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