scholarly journals Quick-Hot-Gram-Cromotropo QHGC lugol solution (Agudelo-Lopez SP and Montoya-Palacio MN, 2004) v1 (protocols.io.bf8fjrtn)

protocols.io ◽  
2020 ◽  
Author(s):  
Javier Antonio
Keyword(s):  
Lugol Solution

scholarly journals Keragaman Plankton dan Kondisi Perairan Tambak Intensif dan Tradisional di Probolinggo Jawa Timur

Biosfera ◽  
2015 ◽  
Vol 32 (2) ◽  
pp. 83 ◽  
Author(s):  
Utojo Utojo
Keyword(s):  
Suspended Solids ◽  
Biological Diversity ◽  
Diversity Index ◽  
Cell Counting ◽  
Community Stability ◽  
Biological Index ◽  
Dominance Index ◽  
Lugol Solution ◽  
Index Value ◽  
Brackishwater Pond

This study aims to estimate the wealth and stability of traditional and intensive brackishwater pond waters through biological index calculation (diversity, uniformity and dominance of plankton) in traditional and intensive brackishwater pond waters of Probolinggo Regency, East Java Province. Sampling plankton and water from the site, considered to represent the diversity of plankton and water stability of traditional and intensive brackishwater pond. Plankton was collected using a plankton net no. 25, then preserved using 1% lugol solution. Measurement of water quality variables include temperature, salinity, dissolved oxygen and pH, whereas the laboratory analyzes that Total Organic Matter (TOM), NO2, NO3, NH3, PO4, Total Suspended Solids (TSS), and Fe. Plankton identification using microscope and calculation with cell counting method. The results of analysis of plankton in intensive brackishwater pond waters are obtained as much as 23 genera consisting of 16 genera of phytoplankton were included into three classes, namely Bacillariophyceae as much as 5 genera, Cyanophyceae 3 genera, Dinophyceae and Chlorophyceae, each of the four genera, whereas zooplankton consisting of 7 genera are included into two classes, namely Crustaceae 6 genera and Rotatoria 1 genus. In traditional brackishwater pond earned as much as 13 genera consisting of 8 genera of phytoplankton were included into Bacillariophyceae 5 genera, Cyanophyceae 2 genera, and Dinophyceae 3 genera, whereas zooplankton consisting of three genera are included into class Crustaceae 2 genera and Rotatoria 1 genus. Plankton abundance in intensive brackishwater pond ranges 702 - 4269 ind./L, diversity index of 0.26 -2.38, uniformity index of 0.10 - 1.5, and dominance index of 0.02 - 0.68. In traditional brackishwater pond have an abundance of plankton range 134 - 776 ind./L, diversity index of 0.10 - 1.97, uniformity index of 0.08 - 1.0, and dominance index of 0.01 - 0.75. Traditional and intensive brackishwater pond waters condition still within the limits that can be tolerated by the culture organism. Based on the index value of biological, diversity of genus in the intensive brackishwater pond is relatively higher than traditional brackishwater pond and the community stability moderate, whereas traditional brackishwater pond unstable. On average, the uniformity of the genus in both  brackishwater ponds are relatively evenly and genus of plankton to dominate the others genus.

scholarly journals Lugol Increases Lipolysis through Upregulation of PPAR-Gamma and Downregulation of C/EBP-Alpha in Mature 3T3-L1 Adipocytes

2020 ◽  
Vol 2020 ◽  
pp. 1-9
Author(s):  
Cuellar-Rufino Sergio ◽  
Zepeda Rossana Citlali ◽  
Flores-Muñoz Mónica ◽  
Santiago-Roque Isela ◽  
Arroyo-Helguera Omar
Keyword(s):  
Transcription Factors ◽  
Culture Medium ◽  
Caspase 3 ◽  
Lethal Dose ◽  
Molecular Iodine ◽  
Ppar Gamma ◽  
Overweight And Obesity ◽  
Intracellular Lipid ◽  
Lugol Solution ◽  
Release Of Glycerol

Overweight and obesity are defined as excessive and abnormal fat accumulation that is harmful to health. This study analyzes the effect of different concentrations of the lugol solution (molecular iodine dissolved in potassium iodide) on lipolysis in cultured 3T3-L1-differentiated adipocytes. The mature adipocytes were treated with doses from 1 to 100 µm of lugol for 0.5, 6, and 24 h. The results showed that mature adipocytes exposed to lugol decrease their viability and increase caspase-3 activity with a lethal dose (LD50) of 473 µm. In mature adipocytes, lugol decreased the total intracellular lipid content, being significant at doses of 10 and 100 µm after 6 and 24 h of treatment (P<0.01), and the accumulation of intracellular triglycerides decreased after 24 h of exposure to lugol (P<0.05). Lugol treatment significantly increases the release of glycerol to the culture medium (P<0.05). The levels of adipocyte-specific transcription factors C/EBP-α were downregulated and PPAR-γ upregulated after 30 min with lugol. These results indicate a lipolytic effect of lugol dependent on PPAR-γ and C/EBP-α expression in mature 3T3-L1 adipocytes.

Toxicity and efficacy profiles of intrathecal injection of I-131 NaI via intraventricular (IVent) or intralumbar (Ilumb) route for leptomeningeal metastases (LM) therapy

2006 ◽  
Vol 24 (18_suppl) ◽  
pp. 1549-1549
Author(s):  
F. C. Wong ◽  
M. Groves ◽  
N. Papadopoulos ◽  
S. Kim ◽  
C. Conrad ◽  
...  
Keyword(s):  
Intrathecal Injection ◽  
Intrathecal Chemotherapy ◽  
Whole Body ◽  
Csf Cytology ◽  
Two Phase ◽  
External Beam Irradiation ◽  
Red Marrow ◽  
Lugol Solution ◽  
Grade Ii ◽  
Low Toxicity

1549 Background: LM is a fatal complication occurring in up to 30% of cancer patients. Intrathecal chemotherapy of LM is limited by diffusion while external beam irradiation is limited by neurotoxicity. Radioiodine I-131 may serve as a ablating agent because of its radiations transverse the meningeal thinkness but spare distant underlying nervous tissues. Two phase I studies are designed to determine MTD and to evaluate potential toxicity and efficacy of intrathecal I-131 sodium iodide (NaI) injected via IVent or the ILumb route. Methods: The IVent or Ilumb protocols each accured 5 groups of patients with cytology-confirmed LM from solid tumors. An injection of 15, 30, 60, 90 or 120 mCi of I-131 NaI was performed after thyroid blockade (Lugol solution and perchlorate), via an Ommaya reservoir (IVent) or lumbar puncture (ILumb). Patients underwent whole-body scintigraphy for biodistribution and radiation dosimetry. Follow-up studies consisted of serial CSF cytology, neuropsychology, ophthalmology, and serum tests (TSH and CBC) to 6 months. Results: The IVent and Ilumb studies accured 16 and 15 patients respectively with LM from melanoma, lung, breast and other tumors (13, 8, 6 and 4 of 31 patients). Because of re-entry into the protocols, one patient received a total of 407 mCi; the maximum radiation absorbed doses were estimated at 3716 cGy in the ventricular CSF and 54 cGy in the red marrow. No treatment-related NCI-Grade II/III/IV neurologic, hematologic or thyroid toxicity is noted. Transient improvements up to 6 weeks are noted in neuropsychologic tests (5/16 and 5/15), ophthalomologic test (3/16 and 1/15) and CSF cytology conversion (to negative) in 5/16 and 4/15 patients, respectively. Electrophysiology tests of 2 patients demonstrated normalization of P37 waves measured by somatosensory evoked potentials along with neuropsychologic test improvements. Conclusions: The MTD has not been reached up to 120 mCi on either the IVent or Ilumb protocol. Intrathecal I-131 NaI is likely to be efficacious to eradicate tumors in the CSF for LM therapy. Because of the low toxicity and likely efficacy, a phase II study with higher dosages is needed to fully explore therapeutic potentials of intrathecal I-131 NaI. No significant financial relationships to disclose.

scholarly journals Effect of Lugol Solution on Thyroid Gland Blood Flow and Microvessel Density in the Patients with Graves’ Disease

2007 ◽  
Vol 92 (6) ◽  
pp. 2182-2189 ◽  
Author(s):  
Yeşim Erbil ◽  
Yasemin Ozluk ◽  
Murat Giriş ◽  
Artur Salmaslıoglu ◽  
Halim Issever ◽  
...  
Keyword(s):  
Clinical Trial ◽  
Blood Flow ◽  
Thyroid Gland ◽  
Blood Loss ◽  
Microvessel Density ◽  
Solution Treatment ◽  
Graves Disease ◽  
Preoperative Treatment ◽  
Cd 34 ◽  
Lugol Solution

Abstract Context: Although some endocrine surgeons administer Lugol solution to decrease thyroid gland vascularity, there is still not an agreement on its effectiveness. Objective: The aims of this clinical trial are to evaluate thyroid blood flow and microvessel density in patients with Graves’ disease who received Lugol solution treatment preoperatively. Design: This was a prospective clinical trial. Setting: This clinical trial took place at a tertiary referral center. Method: Thirty-six patients were randomly assigned to receive either preoperative treatment with Lugol solution (group 1, n = 17) or no preoperative treatment with Lugol solution (group 2, n = 19). Main Outcome Measures: Blood flow through the thyroid arteries of patients with Graves’ disease was measured by color flow Doppler ultrasonography. The microvessel density (MVD) was assessed by immunohistochemical and Western blot analysis of the level of expression of CD-34 in thyroid tissue. The weight and blood loss of the thyroid gland were measured in all patients. Results: The mean blood flow, MVD, CD-34 expression, and blood loss in group 1 patients were significantly lower than those in group 2 patients. There was a negative correlation between Lugol solution treatment and blood flow (rs = −0.629; P = 0.0001), blood loss (rs = −0.621; P = 0.0001), MVD (rs = −0.865; P = 0.0001), and CD-34 expression (rs = −0.865; P = 0.0001). According to logistic regression analysis, Lugol solution treatment resulted in a 9.33-fold decreased rate of intraoperative blood loss. Conclusion: Preoperative Lugol solution treatment decreased the rate of blood flow, thyroid vascularity, and intraoperative blood loss during thyroidectomy.

scholarly journals Evaluation of lugol solution used for counting rumen ciliates

1999 ◽  
Vol 16 (3) ◽  
pp. 725-729 ◽  
Author(s):  
Marta D'Agosto ◽  
Maria Elisa Carneiro
Keyword(s):  
Lugol Solution
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