scholarly journals Antibody Conjugation and CODEX Multiplexed Immunofluorescence of Fresh Frozen Tissue v1

protocols.io ◽  
2021 ◽  
Author(s):  
Elizabeth Neumann ◽  
Carrie not provided Romer ◽  
Nathan Heath Patterson ◽  
Jamie Allen ◽  
Jeff Spraggins
Keyword(s):  
Frozen Tissue ◽  
Fresh Frozen ◽  
Antibody Conjugation

Muscle Biopsy Samples for Histochemical Processing: Alterations Induced by Storage

1988 ◽  
Vol 25 (1) ◽  
pp. 77-82 ◽  
Author(s):  
K. G. Braund ◽  
K. A. Amling
Keyword(s):  
Skeletal Muscle ◽  
Cell Morphology ◽  
Type I ◽  
Type Ii ◽  
Tissue Samples ◽  
Frozen Tissue ◽  
Fresh Frozen ◽  
Healthy Dogs ◽  
Morphology And Morphometry ◽  
Type Ii Fibers

Skeletal muscle samples from two healthy dogs were stored in ice at 0 C for up to 30 hours to examine the influence of time on cell morphology and morphometry. Cytochemical and histochemical properties of muscle to 18 hours were not markedly different from fresh frozen tissue. Samples stored to 30 hours were still satisfactory, despite a decline and unevenness in depth of staining. Morphometry from samples stored at 0 C for 6 hours or longer is not recommended, due to the statistically significant increase in diameter (from 21 to 25%) of type I and type II fibers.

scholarly journals Multiplex Immunofluorescence on Fresh Frozen Tissue v1 (protocols.io.665hhg6)

protocols.io ◽  
2019 ◽  
Author(s):  
Maya Brewer ◽  
Yuantee Zhu ◽  
Elizabeth Neumann ◽  
Danielle Gutierrez ◽  
Jeff Spraggins ◽  
...  
Keyword(s):  
Frozen Tissue ◽  
Fresh Frozen

Intermediate Filaments in Fresh Frozen Tissue in Neuro-Oncology — About a Series of 1000 Peroperative Biopsies —

1987 ◽  
pp. 55-60
Author(s):  
Y. Krivosic ◽  
M. Jomin ◽  
J. L. Christiaens ◽  
P. Dhellemmes ◽  
G. Combelles ◽  
...  
Keyword(s):  
Intermediate Filaments ◽  
Frozen Tissue ◽  
Fresh Frozen

scholarly journals Brief Report: Simplified Myeloperoxidase Stain Using Benzidine Dihydrochloride

Blood ◽  
1965 ◽  
Vol 26 (2) ◽  
pp. 215-219 ◽  
Author(s):  
LEONARD S. KAPLOW ◽  
Charlotte Ladd
Keyword(s):  
Peroxidase Activity ◽  
Incubation Mixture ◽  
Tissue Sections ◽  
Blood Smears ◽  
Highly Sensitive ◽  
Frozen Tissue ◽  
Fresh Frozen

Abstract A method is described for demonstrating leukocyte peroxidase activity in which benzidine dihydrochloride is used as the indicator compound instead of the more commonly used but potentially more hazardous benzidine base. The method is highly sensitive and rapid and permits the use of fixed blood smears and organ imprints. The incubation mixture, which incorporates safranin as a counterstain, may be used over and over again, for as long as 6 months. The method is also applicable to fresh frozen tissue sections.

scholarly journals Comparison of Accuracy of Whole-Exome Sequencing with Formalin-Fixed Paraffin-Embedded and Fresh Frozen Tissue Samples

PLoS ONE ◽  
2015 ◽  
Vol 10 (12) ◽  
pp. e0144162 ◽  
Author(s):  
Ensel Oh ◽  
Yoon-La Choi ◽  
Mi Jeong Kwon ◽  
Ryong Nam Kim ◽  
Yu Jin Kim ◽  
...  
Keyword(s):  
Exome Sequencing ◽  
Whole Exome Sequencing ◽  
Tissue Samples ◽  
Whole Exome ◽  
Formalin Fixed Paraffin ◽  
Frozen Tissue ◽  
Formalin Fixed Paraffin Embedded ◽  
Fresh Frozen ◽  
Formalin Fixed

scholarly journals Multicenter fresh frozen tissue sampling in colorectal cancer: does the quality meet the standards for state of the art biomarker research?

2017 ◽  
Vol 18 (3) ◽  
pp. 425-431 ◽  
Author(s):  
Z. S. Lalmahomed ◽  
◽  
R. R. J. Coebergh van den Braak ◽  
M. H. A. Oomen ◽  
S. P. Arshad ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
State Of The Art ◽  
Tissue Sampling ◽  
Frozen Tissue ◽  
Fresh Frozen ◽  
Biomarker Research

scholarly journals Vapor fixation for immunocytochemistry and X-ray microanalysis on cryoultramicrotome sections.

1984 ◽  
Vol 32 (6) ◽  
pp. 636-642 ◽  
Author(s):  
P M Frederik ◽  
P H Bomans ◽  
W M Busing ◽  
R Odselius ◽  
W M Hax
Keyword(s):  
Exocrine Pancreas ◽  
Osmium Tetroxide ◽  
Protein A ◽  
Alpha Amylase ◽  
X Ray ◽  
Freeze Dried ◽  
Frozen Tissue ◽  
Formaldehyde Vapor ◽  
Fresh Frozen ◽  
Subcellular Level

Several organic and inorganic vapor fixatives have been tested for their ability to stabilize the ultrastructure of freeze-dried thin cryosections. The vapors from osmium tetroxide and dry formaldehyde gave a good preservation of the ultrastructure. Fixation in formaldehyde vapor preserved the immunoreactivity of alpha-amylase in exocrine pancreas, as was demonstrated with an indirect labeling technique using anti-alpha-amylase and protein A-gold. A major advantage of the use of vapor fixation is that cryosections from a specimen of fresh-frozen tissue can be used for immunocytochemistry as well as for X-ray microanalysis, as was demonstrated for the exocrine pancreas. This opens the possibility of localizing atomic species (X-ray microanalysis) and molecular species (immunocytochemistry) at the subcellular level on thin cryosections from the same tissue block.

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