Cyanobacterial OD based Growth Assay - iGEM IISER Pune 2021 v1

1982 ◽

Vol 40 ◽

pp. 132-133

Author(s):

W.T. Gunning ◽

M.R. Marino ◽

M.S. Babcock ◽

G.D. Stoner

Keyword(s):

Epithelial Cells ◽

Cell Types ◽

Replication Rate ◽

Enzymatic Dissociation ◽

Growth Assay ◽

Epidermal Growth ◽

Fetal Bovine ◽

Esophageal Epithelial Cells ◽

Cellular Replication

The role of calcium in modulating cellular replication and differentiation has been described for various cell types. In the present study, the effects of Ca++ on the growth and differentiation of cultured rat esophageal epithelial cells was investigated.Epithelial cells were isolated from esophagi taken from 8 week-old male CDF rats by the enzymatic dissociation method of Kaighn. The cells were cultured in PFMR-4 medium supplemented with 0.25 mg/ml dialyzed fetal bovine serum, 5 ng/ml epidermal growth factor, 10-6 M hydrocortisone 10-6 M phosphoethanolamine, 10-6 M ethanolamine, 5 pg/ml insulin, 5 ng/ml transferrin, 10 ng/ml cholera toxin and 50 ng/ml garamycin at 36.5°C in a humidified atmosphere of 3% CO2 in air. At weekly intervals, the cells were subcultured with a solution containing 1% polyvinylpyrrolidone, 0.01% EGTA, and 0.05% trypsin. After various passages, the replication rate of the cells in PFMR-4 medium containing from 10-6 M to 10-3 M Ca++ was determined using a clonal growth assay.

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Combination of Suspension Array and Mycelial Growth Assay for Detecting Multiple-Fungicide Resistance in Botrytis cinerea in Hebei Province in China

Plant Disease ◽

10.1094/pdis-07-18-1269-re ◽

2019 ◽

Vol 103 (6) ◽

pp. 1213-1219

Author(s):

Zehua Su ◽

Xin Zhang ◽

Jianjiang Zhao ◽

Wenqiao Wang ◽

Lei Shang ◽

...

Keyword(s):

Botrytis Cinerea ◽

High Throughput ◽

Fungicide Resistance ◽

Mycelial Growth ◽

Accurate Method ◽

Hebei Province ◽

Combination Method ◽

Major Type ◽

Suspension Array ◽

Growth Assay

To provide a high-throughput, efficient, and accurate method to monitor multiple-fungicide resistance of Botrytis cinerea in the field, we used the suspension array, sequencing, and mycelial growth assay in our research. Discriminating-dose bioassays for detecting carbendazim, diethofencarb, boscalid, and iprodione resistance (CarR, DieR, BosR, and IprR, respectively) were used to analyze 257 isolates collected from Hebei Province in China during 2016 and 2017. High resistance frequencies to carbendazim (100%), diethofencarb (92.08%), and iprodione (86.59%) were detected. BosR isolates accounted for 11.67% of the total. In addition, 103 isolates were randomly selected for phenotype and genotype detection. The high-throughput suspension array was utilized to detect eight genotypes simultaneously, including BenA-E198, BenA-198A, SdhB-H272, SdhB-272Y, BcOS1-I365, BcOS1-365S, erg27-F412, and erg27-412S, which were associated with resistance toward carbendazim or diethofencarb, boscalid, iprodione, and fenhexamid (FenR), respectively. Most of the benzimidazole-resistant isolates (81.55%) possessed the E198V mutation in the BenA gene. Ninety-three isolates with dual resistance to carbendazim and diethofencarb showed the E198V/K mutation. All BosR isolates carried the H272R mutation in the SdhB gene. The I365S and Q369P+N373S (66.99%) mutations in the BcOS1 gene were more frequently observed. No mutation was detected in the erg27 gene in Hebei isolates. There were 13 resistance profile phenotypes. Phenotypes with triple resistance were the most common (83.50%), and CarRDieRBosSIprRFenS was the major type. CarR isolates that carried E198V/K/A were all highly resistant (HR) and only one F200Y mutant was moderately resistant (MR) to carbendazim. Isolates that possessed E198V/K were MR or HR to diethofencarb. BosR isolates that possessed H272R mutation were lowly resistant (LR). IprR isolates were all LR or MR. The distribution of half maximal effective concentrations of CarR isolates with E198V/K mutations and IprR isolates with Q369P+N373S mutations significantly increased from 2016 to 2017. Combined with our observations, a combination method of the high-throughput suspension array and the mycelial growth assay was suggested to accurately monitor multiple resistance of B. cinerea in the field.

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Comparison of clinical acinetobacter strains using a carbon source growth assay

Epidemiology and Infection ◽

10.1017/s0950268800047452 ◽

1990 ◽

Vol 104 (3) ◽

pp. 443-453 ◽

Cited By ~ 9

Author(s):

L. Dijkshoorn ◽

A. Van Ooyen ◽

W. C. J. Hop ◽

M. Theuns ◽

M. F. Michel

Keyword(s):

Cluster Analysis ◽

Carbon Source ◽

Univariate Analysis ◽

Carbon Sources ◽

Protein Profiles ◽

Growth Data ◽

Epidemiological Factors ◽

Growth Assay ◽

Growth Profiles ◽

Related Pattern

SUMMARYA quantitative carbon source growth assay, comprising ten carbon sources, was used to compare acinetobacter strains from three hospitals. The strains had been obtained during episodes of increased prevalence of isolations and were, for each hospital, assumed to be epidemiologically related. This assumption was supported by the electrophoretic protein profiles of the strains. Univariate analysis of growth data showed significant differences between strains from the three hospitals. Moreover, cluster analysis revealed that the major pattern in the data was related to the epidemiological origin of the strains. Exceptions to the epidemic-related pattern were observed. Thus, apart from epidemiological factors, other factors might contribute to carbon source growth profiles of the strains. It is concluded that the carbon growth assay may be useful to distinguish roughly between acinetobacter strains from different sites of origin. Further studies are required to analyse additional factors which influence carbon source growth of strains.

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QSTR mathematical models for the toxicity of aliphatic carboxylic acids on tetrahymena pyriformis

Creative Mathematics and Informatics ◽

10.37193/cmi.2013.02.09 ◽

2013 ◽

Vol 22 (2) ◽

Author(s):

Zoița Mărioara Berinde

Keyword(s):

Dissociation Constant ◽

Carboxylic Acids ◽

Aquatic Toxicity ◽

Tetrahymena Pyriformis ◽

Refraction Index ◽

Linear Regression Models ◽

Acidic Dissociation ◽

Growth Assay ◽

Aliphatic Carboxylic Acids ◽

Acidic Dissociation Constant

The present work represents an attempt to improve QSTR models for aquatic toxicity of 3838 aliphatic carboxylic acids tested in the Tetrahymena pyriformis population growth assay, using the topological index ZEP and the following nine main electrotopological and molecular descriptors: acidic dissociation constant, 1-octanol/water partition coefficient, the energy of the lowest unoccupied molecular orbital, acidic dissociation constant, molar refractivity, refraction index, surface tension, polarizability and, electrotopological states. Several different relations between toxicity [loglog(IGC50-1)] and the molecular and topological properties were examined, and a group of multiple linear regression models with high fitness scores were generated.

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O-45 Drug sensitivity test (in vitro growth assay) of gefitinib (Iressa, ZD1839) for primary lung cancer

Lung Cancer ◽

10.1016/s0169-5002(03)91703-x ◽

2003 ◽

Vol 41 ◽

pp. S16

Author(s):

Motoshi Takao ◽

Kentaro Inoue ◽

Fumiaki Watanabe ◽

Tomohito Tarukawa ◽

Masanori Kaneda ◽

...

Keyword(s):

Lung Cancer ◽

Drug Sensitivity ◽

Primary Lung Cancer ◽

Sensitivity Test ◽

In Vitro Growth ◽

Drug Sensitivity Test ◽

Growth Assay

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Sensitivity of Rhizoctonia solani AG-2-2 from Sugar Beet to Fungicides

Plant Disease ◽

10.1094/pdis-04-16-0525-re ◽

2016 ◽

Vol 100 (12) ◽

pp. 2427-2433 ◽

Cited By ~ 4

Author(s):

Sahar Arabiat ◽

Mohamed F. R. Khan

Keyword(s):

Sugar Beet ◽

Rhizoctonia Solani ◽

Root Rot ◽

The United States ◽

Damping Off ◽

Growth Assay ◽

The Mean ◽

Crown And Root Rot ◽

Mean Concentration

Rhizoctonia damping-off and crown and root rot caused by Rhizoctonia solani are major diseases of sugar beet (Beta vulgaris L.) worldwide, and growers in the United States rely on fungicides for disease management. Sensitivity of R. solani to fungicides was evaluated in vitro using a mycelial radial growth assay and by evaluating disease severity on R. solani AG 2-2 inoculated plants treated with fungicides in the greenhouse. The mean concentration that caused 50% mycelial growth inhibition (EC50) values for baseline isolates (collected before the fungicides were registered for sugar beet) were 49.7, 97.1, 0.3, 0.2, and 0.9 μg ml−1 and for nonbaseline isolates (collected after registration and use of fungicides) were 296.1, 341.7, 0.9, 0.2, and 0.6 μg ml−1 for azoxystrobin, trifloxystrobin, pyraclostrobin, penthiopyrad, and prothioconazole, respectively. The mean EC50 values of azoxystrobin, trifloxystrobin, and pyraclostrobin significantly increased in the nonbaseline isolates compared with baseline isolates, with a resistant factor of 6.0, 3.5, and 3.0, respectively. Frequency of isolates with EC50 values >10 μg ml−1 for azoxystrobin and trifloxystrobin increased from 25% in baseline isolates to 80% in nonbaseline isolates. Although sensitivity of nonbaseline isolates of R. solani to quinone outside inhibitors decreased, these fungicides at labeled rates were still effective at controlling the pathogen under greenhouse conditions.

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Measurement of the Amino Acid Content of Fish Meal Proteins by Chick Growth Assay

Poultry Science ◽

10.3382/ps.0440408 ◽

1965 ◽

Vol 44 (2) ◽

pp. 408-413 ◽

Cited By ~ 7

Author(s):

R.E. Smith ◽

H.M. Scott

Keyword(s):

Amino Acid ◽

Acid Content ◽

Fish Meal ◽

Amino Acid Content ◽

Chick Growth ◽

Growth Assay

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Protein Efficiency Ratio of Pigeon Pea (Cajanus cajan) and Lima Bean (Phaseolus lunatus): A Sprague-Dawley Rat Growth Assay

Acta Medica Philippina ◽

10.47895/amp.v52i3.391 ◽

2018 ◽

Vol 52 (3) ◽

Author(s):

Raycha Lei Concess M. Rama ◽

Jillean Camille Q. Fabi ◽

Generose Christy M. Mateo ◽

Jewel Amor C. Catubag ◽

Lemuel L. Lozada ◽

...

Keyword(s):

Cajanus Cajan ◽

Protein Efficiency Ratio ◽

Lima Bean ◽

Protein Digestibility ◽

Pigeon Pea ◽

Phaseolus Lunatus ◽

Sprague Dawley ◽

Efficiency Ratio ◽

Weanling Rats ◽

Growth Assay

Objective. This study has been conducted to determine the protein efficiency ratio (PER) of selected indigenous legumes, namely pigeon pea (Cajanus cajan) and lima bean (Phaseolus lunatus), as alternative protein sources. Methods. Experimental diets (pigeon pea-based and lima bean-based) and control diet (casein-based) were formulated and standardized using proximate analysis to have 10% protein basal diet based on the Association of Official Analytical Chemists (AOAC) guidelines. Diets were given to corresponding groups (2 experimental, 1 control) using 30 albino Sprague-Dawley weanling rats aged 21 days old, over a 28-day growth assay. Results. Results showed that the mean final weight and weight gain were significantly higher in rats fed with caseinbased diet (p<0.05 for both parameters) compared to those fed with pigeon pea-based diet and lima bean-based diet. The two experimental groups showed no significant difference between their mean final weights and weight changes (p=1, p>0.05). The PER of the casein (3.37 ± 2.71) is higher than that of the pigeon pea (1.87) and lima bean (1.32). These results can be attributed to the presence of anti-nutritional factors (ANFs) in the seeds of pigeon pea and lima bean. Reduced protein intake (due to bitter taste), toxicity, and interference with protein digestibility (through interaction with digestive enzymes) imparted by these ANFs can explain the weight loss among the experimental groups and consequently the low PER. Heat treatment done in the study were not adequate to remove the ANFs. Conclusion. The study shows that pigeon pea and lima bean as protein sources alone are not as adequate and efficient in providing necessary protein requirements for weanling rats. Proper processing and treatment should be done to remove inhibitors of protein digestibility and quality.

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