Effect of Protein Diet on Growth Performance and Body Composition of Labeo rohita Reared in Glass Tank

Fish is used as a great source of food and the quality of its meat can be indicated by fat and protein contents as well as low quantity of water in fish meat. Therefore, the present study was performed for improvement in weight, feed-conversion ratio, protein-efficiency ratio, and growth. The experiment was performed at commercial level in field (pond) and the influence of fish meal protein on growth of Labeo rohita was investigated in 60 days culture trials. 90 fingerlings (Size: 4.97 to 6.04; Weight: 4.15 to 4.20) were maintained in well aerated 280 L three glass tanks in triplicate. These glass tanks were named as T1, T2 and T3. T1 was consisted of 30% crude protein with control diet containing proteins, fats vitamins and fibres, T2 consisted of 35% protein with control diet, while T3 consisted of only control diet (rice polish). The fish were fed 4% percent of their body weight twice a day at twelve hours interval for 60 days. There was a significant increase (p<0.05) in body weight, feed-conversion ratio, protein-efficiency ratio, and gross fish production of fish having 35% fish meal protein with control diet (T2) when compared with 30% fish meal protein with control diet T1 and control diet T3. These findings suggest that 35% fishmeal protein appear to be sufficient for obtaining optimum growth in fish.

Effect of dietary lipid levels on growth, body composition, and enzyme activities of larvae of butter catfish, Ompok bimaculatus (Actinopterygii: Siluriformes: Siluridae)

The Indian butter catfish, Ompok bimaculatus (Bloch, 1794), is a high-value catfish that has gained immense consumer preference in South-East Asia. However, information on the nutritional requirements of this species is scanty. Hence, an experiment was conducted to evaluate the effects of varying dietary lipid levels on growth, body composition, and activities of digestive and metabolic enzymes in larvae. Three isonitrogenous (40% crude protein) diets were formulated by supplementing fish and vegetable oil (1:1) at 4.5% (D1), 7% (D2), and 9.5% (D3) levels (containing crude lipid 5.7%, 8.0%, and 10.45%, respectively in diets D1–D3) to a fish meal- and oilcake-based formulated diet. Experimental diets were fed to butter catfish larvae (0.15 ± 0.01 g) in triplicate groups for a period of 42 days. Proximate compositions of the experimental diets, as well as fish carcass, were analyzed using standard procedures (AOAC 2005). Digestive and metabolic enzyme activities were analyzed at the completion of the experiment by standard methodology. Butter catfish larvae fed the diet D2 (8% crude lipid) resulted in the best performance in terms of weight gain (final weight 1.40 ± 0.07 g), net weight gain (1.31 ± 0.06 g), specific growth rate (5.50 ± 0.05% · day−1), and protein efficiency ratio (2.39 ± 0.17). The highest lipid deposition (2.90 ± 0.12%) in the carcass was also recorded in fish reared on diet D2. The final weight, net weight gain, protein efficiency ratio, and specific growth rate were significantly (P &lt; 0.05) higher in D2 having 8% lipid. Moisture and lipid contents of the whole body were significantly (P &lt; 0.05) higher in larvae fed diet D2. Amylase activity in fish significantly (P &lt; 0.05) decreased with increasing dietary lipid levels. The maximum alkaline protease, pepsin, and lipase activities were noticed in the larvae fed diet D2. Progressive decrease in liver glucose-6-phosphate dehydrogenase activities and significant increase (P &lt; 0.05) in the activities of neoglucogenic enzymes (glucose-6-phosphatase and fructose-1,6-bis phosphatase) were noticed with an increase in dietary lipid levels. Significantly lower (P &lt; 0.05) activities of LDH, ALT, and AST were recorded in the group fed diet D2. Results of the study indicated that 8% crude lipid in the diet could assure optimum growth and survival of butter catfish larvae during early development. An appraisal on growth, body composition, and digestive as well as metabolic function in the butter catfish larvae recorded in the study might provide some important information to consider application of formulated diets for the larviculture of Ompok bimaculatus.

Effect of dietary lipid levels on growth, body composition, and enzyme activities of larvae of butter catfish, Ompok bimaculatus (Actinopterygii: Siluriformes: Siluridae)

The Indian butter catfish, Ompok bimaculatus (Bloch, 1794), is a high-value catfish that has gained immense consumer preference in South-East Asia. However, information on the nutritional requirements of this species is scanty. Hence, an experiment was conducted to evaluate the effects of varying dietary lipid levels on growth, body composition, and activities of digestive and metabolic enzymes in larvae. Three isonitrogenous (40% crude protein) diets were formulated by supplementing fish and vegetable oil (1:1) at 4.5% (D1), 7% (D2), and 9.5% (D3) levels (containing crude lipid 5.7%, 8.0%, and 10.45%, respectively in diets D1–D3) to a fish meal- and oilcake-based formulated diet. Experimental diets were fed to butter catfish larvae (0.15 ± 0.01 g) in triplicate groups for a period of 42 days. Proximate compositions of the experimental diets, as well as fish carcass, were analyzed using standard procedures (AOAC 2005). Digestive and metabolic enzyme activities were analyzed at the completion of the experiment by standard methodology. Butter catfish larvae fed the diet D2 (8% crude lipid) resulted in the best performance in terms of weight gain (final weight 1.40 ± 0.07 g), net weight gain (1.31 ± 0.06 g), specific growth rate (5.50 ± 0.05% · day−1), and protein efficiency ratio (2.39 ± 0.17). The highest lipid deposition (2.90 ± 0.12%) in the carcass was also recorded in fish reared on diet D2. The final weight, net weight gain, protein efficiency ratio, and specific growth rate were significantly (P &lt; 0.05) higher in D2 having 8% lipid. Moisture and lipid contents of the whole body were significantly (P &lt; 0.05) higher in larvae fed diet D2. Amylase activity in fish significantly (P &lt; 0.05) decreased with increasing dietary lipid levels. The maximum alkaline protease, pepsin, and lipase activities were noticed in the larvae fed diet D2. Progressive decrease in liver glucose-6-phosphate dehydrogenase activities and significant increase (P &lt; 0.05) in the activities of neoglucogenic enzymes (glucose-6-phosphatase and fructose-1,6-bis phosphatase) were noticed with an increase in dietary lipid levels. Significantly lower (P &lt; 0.05) activities of LDH, ALT, and AST were recorded in the group fed diet D2. Results of the study indicated that 8% crude lipid in the diet could assure optimum growth and survival of butter catfish larvae during early development. An appraisal on growth, body composition, and digestive as well as metabolic function in the butter catfish larvae recorded in the study might provide some important information to consider application of formulated diets for the larviculture of Ompok bimaculatus.

Studies on growth performance of Cyprinus carpio var. Communis fed with Bacillus clausii supplemented diets

The present investigation entitled “Studies on growth performance of Cyprinus carpio var. Communis fed with Bacillus clausii supplemented diets” was carried out at Faculty of Fisheries, Ganderbal over a period of 60 days from April 1st to June Ist, 2019. The fingerlings were fed with dry diets containing three concentrations of probiotic viz., (T1: 1%, T2: 1.5%, T3: 2%) and control group (T0: containing no probiotic). The growth parameters including per cent weight gain, Specific growth rate (SGR), feed conversion ratio (FCR), feed efficiency ratio (FER) and protein efficiency ratio (PER) were measured at the end of the experiment. The results showed that the probiotic incorporated diets had significant impact on growth than control group (P<0.05). The highest values of treatment T2 with 2 per cent probiotic showed the highest percent weight gain (74.62%), specific growth rate (0.40), feed efficiency ratio (0.90), protein efficiency ratio (1.82) were observed in treatments with 2% concentration of probiotic where as, feed conversion ratio(1.09) was lower in groups received probiotic through diets than control.

Konsep Dasar Ilmu Gizi

Pengertian dari ilmu gizi (nutrition science) adalah “ Ilmu yg mempelajari segala sesuatu tentang makanan dalam hubungannya dengan kesehatan optimal”. Karbohidrat merupakan senyawa organik yang terdiri dari unsur karbon (C), hidrogen (H), dan oksigen(O) . Fungsi Karbohidrat adalah sebagai Sumber energi, pemberi rasa manis pada makanan, penghemat protein, pengatur metabolisme lemak, membantu pengeluaran feses. Lemak adalah senyawa organik yang larut dalam pelarut non polar seperti etanol, kloroform dan benzena, tetapi tidak larut dalam air. Lemak mengandung karbon , hidrogen dan oksigen. Fungsi umum lemak di dalam tubuh adalah menghasilkan energi terkonsentrasi yang memberikan kalori sebesar 9 kal/gram.Protein terdiri atas rantai-rantai panjang asam amino yang terikat satu sama lain dalam ikatan peptide. Asam amino terdiri atas unsur-unsur karbon, hidrogen, oksigen dan nitrogen, unsur nitrogen adalah unsur utama protein, Mutu protein ditentukan melalui : Nilai Biologik (NB), Net Protein Utilization (NPU), Protein Efficiency Ratio (PER), dan Skor Kimia (Skor Asam Amino). Vitamin adalah senyawa organik yang tersusun dari karbon, hidrogen, oksigen, dan terkadang nitrogen atau elemen lain yang dibutuhkan dalam jumlah kecil agar metabolisme, pertumbuhan dan perkembangan berjalan normal. Vitamin dapat dipilahkan menjadi 2 kelompok yaitu kelompok yang larut dalam lemak dan yang larut dalam air. Mineral berdasarkan kebutuhan digolongkan menjadi mineral makro dan mineral mikro.

Effects of digestible amino acid based formulation of low protein broiler diets supplemented with valine, isoleucine and arginine on performance and protein efficiency

The aim of the present study was to investigate the effect of digestible amino acid (DAA) based formulation strategy, and l-valine (l-Val), l-isoleucine (l-Ile) and l-arginine (l-Arg) supplementation to reduce the crude protein (CP) level of broiler diets on performance, carcass characteristics and protein efficiency ratio by comparing with the control diet formulated on total amino acid base. A total of 792 one-day-old Ross 308 broiler chicks were divided into 48 floor pens, with 24 pens containing 16 chicks and 24 pens containing 17 chicks. The experiment was organized in a completely randomized block design with four dietary treatments as follows; T1: formulated to meet minimum both total amino acid and DAA requirements without using l-Val, l-Ile, and l-Arg, T2: formulated to meet DAA requirements without using l-Val, l-Ile and l-Arg, T3: formulated to meet DAA requirements by using l-Val alone, T4: formulated to meet DAA requirements by using l-Val, l-Arg, and l-Ile. Each treatment had 12 replicates. Neither l-Val (T3) nor l-Val, l-Ile, and l-Arg supplemented (T4) low CP dietary treatments had any negative impact on growth performance, feed conversion ratio during experimental periods, as well as carcass parameters of broilers. Indeed, CP reduction by the addition of l-Val alone or l-Val, l-Ile, and l-Arg together even resulted in a significant improvement in protein efficiency ratio compared to T1 and T2 treatments. Supplementation of l-Val either alone or along with l-Ile and l-Arg to diets formulated based on DAA not only decreased dietary CP but also soybean meal inclusion. It can be concluded that maintained growth performance, and even improved protein utilization can be achieved due to the DAA based formulation strategy and supplementing broiler diets with l-Val alone or together with l-Ile and l-Arg.

Performance of Growing Rabbits Fed Diets Containing Graded Levels of Gmelina (Gmelina arborea) Fruit Pulp Meal

The study involved the recycling of gmelina fruit pulp to animal feed to reduce environmental pollution by evaluating the feed intake and body weight changes of rabbits fed diets incorporated with Ripe Gmelina Fruit Pulp (RGFP) at 0, 25, 50, 75 and 100% in replacement of maize as groups I, II, III, IV and V, respectively. Sixty (60) rabbits in five groups of 12 animals each were used for the eight-week study in completely randomized design experiment. Initial weight, final weight, weight gain, feed intake (concentrate + forage), concentrate intake and forage intake ranged from 575–575.02g, 1605–1900g, 1029.98–1324.99g, 8130–7569.85g, 5711.33–1177.11g, 6392.74–2418.67g, respectively. All these parameters were significantly affected (p<0.05) by inclusion of RGFP except initial weight. Feed conversion ratio (FCR), water intake (ml), water–feed ratio and protein efficiency ratio ranged from 7.35–6.14, 278.34–203.36, 0.034–0.027 and 0.8–0.53, respectively. These parameters were also significantly affected (p<0.05) by inclusion of RGFP in diets except water–feed ratio and protein efficiency ratio. Weight gain, total feed intake (concentrate + forage), concentrate intake and water intake of rabbits fed 0% RGFP were the highest (p<0.05) while the 100% RGFP group recorded the least (p<0.05). Weight gain and total feed intake of 0% RGFP group was similar to those of groups 25%, and 50%. The 100% RGFP fed rabbits had the highest (p<0.05) forage intake but least (p<0.05) FCR while 0% RGFP fed group had the least (p<0.05) forage intake and highest (p<0.05) FCR. Weight gain, total feed intake (concentrate + forage), concentrate intake and water intake significantly (p<0.05) decreased as RGFP increased while FCR and forage intake increased significantly (p<0.05) as RGFP increased. This study suggests that RGFP could replace up to 50% of maize, beyond which the animals start losing weight. Keywords: Hindgut fermenter, Gmelina fruit waste, rabbit nutrition, recycling

Food Grains and Jaggery-based Expanded Food: Optimization of Process Variables, Protein Efficiency Ratio and Consumer Acceptability

Extrusion parameters for nutritious expanded food with jaggery were optimized following box-benken design using Response Surface Methodology. Jaggery and feed moisture affected physical properties, while nutritional and sensory properties of products were influenced by jaggery only. Optimized level of extrusion parameters consisted of 100 C die head temperature, screw speed of 304 rpm, 14 % moisture and 4 g jaggery per 100 g of formulation with 80 % maize, 14 % defatted soy-meal, and 6 % sesame-based formulation having overall desirability of 0.807. This expanded food showed expansion ratio of 3.57, 173.76 kg.m-3 bulk density, 15.18 % protein with 72.55 % invitro protein digestibility and 2.63 protein efficiency ratio, 1.97 % total minerals, 2.9 mg.100 g-1 iron, 158 mg.100 g-1 calcium with acceptability score of 8.2 that also indicated the consumer acceptability ≥7 by 95.37 % using 9-point hedonic scale.

Performance and carcass characteristics of weaner pigs fed dietary raw bambarra nut offal

A 56-day feeding trial using twelve (12) crossbred weaner pigs of 8 weeks of age and average weight of 10.58±17kg were used to investigate the effect of different dietary levels of raw bambara groundnut offal on their growth and carcass characteristics. Four diets were formulated to contain raw bambara groundnut offal at 0, 10, 20 and 30%, designated T1, T2, T3 and T4 respectively. The weaner pigs were randomly assigned to the four (4) diets in a completely randomized design (CRD) experiment. Chemical composition of the test ingredient and diets were analysed. Data collected include feed intake, weight gain, feed conversion ratio (FCR), protein efficiency ratio (PER) and economics of production. The carcass characteristics were also evaluated. The proximate composition of the experimental diets revealed that the crude protein and ether extract contents of the diets decreased with increased levels of raw BGO in the diets, but the converse was true for crude fibre and ash contents. The results indicated significant differences (p< 0.05) in daily weight gain (DWG). feed conversion ratio, protein efficiency ratio, cost (N) /kg of seed and cost (N)/kg weight gain. The values were. 0.34, 0.29, 0.28 and 0.22kg for DWG, 3.03, 3.62, 3.55 and 4.30 for FCR in T1, T2, T3 and T4 respectively. Protein efficiency ratio, values were 1.84, 1.54, 1.57 and 1.29. Cost/kg of feed values were 75.69, 70.13, 64.56 and 56.10, and cost (N)/kg weight gain were 229.32, 253.86, 229.20 and 253.69 for the treatments respectively. The results of the carcass evaluation indicated significant differences (p< 0.05) for ham and trotters. The values were 0.31, 0.35, 0.37 and 0.32 for ham in T1, T2, T3 and T4 respectively. The values of trotters were, 64.6.56.99, 56.98 and 53.66 among the treatments. For organs characteristics, the results indicated significant differences (p< 0.05) for lung, kidney, liver and spleen. The values of the lungs were, 11.20. 11.25. 9.40 and 8.97 among the treatments. The values of kidney and liver were, 3.34, 2.86, 3.72 and 3.14, and 24.28, 28.57, 22.55 and 27.70 respectively. The inclusion of up to 20% raw BGO in the diet of weaner pigs recorded comparable daily weight gain, feed conversion ratio and cost/kg weight gain with the group fed the control (0%raw BGO). Further studies involving heat treatment of the BGO might be necessary to improved nutrient utilization by the animals, and also increase inclusion level.

Growth performance and haematological traits of Nigerian local chickens fed varied dietary protein levels

This study was carried out to investigate the growth performance and haematological traits of Nigerian local chickens (NLC) fed varied dietary protein levels. An eleven week feeding trial was done in a 3 x 2 factorial arrangement using six treatment groups comprising a combination of two genotypes of the NLC' identified as normal leather (na) and naked neck (Na) and three dietary protein levels - high protein: HP (22% and 20% CP chick mash and grower mash respectively), medium protein: MP (20% and 18% chick mash and grower mash respectively) and low protein: LP (18% and 16% chick mash and grower mash). Data were collected on growth performance traits (body weight, feed intake, feed conversion ratio and protein efficiency ratio). At the end of the feeding trial a haematological assay was done. Neither genotype nor interaction of genotype and dietary protein level influenced (p>0.05) any of the growth performance traits studied. Birds on HP ate 10.21% and 7.09% less feed than those on MP and LP respectively. A better (p<0.05) protein efficiency ratio (up 10 20.79%) was observed in birds fed LP. Na birds had 4.55% and 17.50x704L-1 more packed cell volume and platelets respectively than na birds (p<0.05). All haematological traits studied, except white blood cell count, showed significant variations (p<0.05) due to varied dietary protein levels. Higher haemoglobin, red blood cell counts, mean corpuscular haemoglobin concentration and platelets were observed in birds fed LP diet. Reducing the dietary protein level had no adverse effect on the NLC thus, the LP diet is recommended for raising the Nigerian local chicken.