Proteomics workflow for APP/Aβ TOMAHAQ analysis in endosomal and lysosomal fractions v2
Keyword(s):
The ability to detect processing of APP to the Ab amyloid peptide is challenging. This protocols describes methods for analysis of Ab "half-tryptic" peptides from purified organelles (endosomes and lysosomes). The targeted proteomics approach using TOMAHAQ coupled with Tomahto, which is an API for use on a Thermo orbitrap instrument that facilitates detection of trigger peptides and fragmentation of target peptide reporter ions.
2012 ◽
Vol 11
(8)
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pp. 394-410
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2014 ◽
Vol 349
(2)
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pp. 221-228
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2016 ◽
Vol 15
(2)
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pp. 638-646
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2016 ◽
Vol 122
(5)
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pp. 281-288
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2012 ◽
Vol 12
(1)
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pp. 55-64
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Keyword(s):