scholarly journals Isolation of human islet cells, culture with heparan sulfate mimetics and flow cytometry analysis of beta cell viability v1 (protocols.io.kwwcxfe)

protocols.io ◽  
2017 ◽  
Author(s):  
Sarah Popp ◽  
Charmaine Simeonovic
Keyword(s):  
Flow Cytometry ◽  
Beta Cell ◽  
Cell Viability ◽  
Heparan Sulfate ◽  
Islet Cells ◽  
Human Islet ◽  
Flow Cytometry Analysis ◽  
Beta Cell Viability

BENEFICIAL EFFECT OF HUMAN ISLET RESCUE GRADIENT PURIFICATION ON EMBEDDED ISLET AND FRACTIONAL BETA CELL VIABILITY

2008 ◽  
Vol 86 (Supplement) ◽  
pp. 571
Author(s):  
A Miki ◽  
C Ricordi ◽  
T Yamamoto ◽  
A Mita ◽  
S Barker ◽  
...  
Keyword(s):  
Beta Cell ◽  
Beneficial Effect ◽  
Cell Viability ◽  
Human Islet ◽  
Beta Cell Viability

scholarly journals Effect of Human Islet Rescue Gradient Purification on Islet Yield and Fractional Beta Cell Viability

2008 ◽  
Vol 40 (2) ◽  
pp. 360-361 ◽  
Author(s):  
A. Miki ◽  
C. Ricordi ◽  
T. Yamamoto ◽  
A. Mita ◽  
S. Barker ◽  
...  
Keyword(s):  
Beta Cell ◽  
Cell Viability ◽  
Human Islet ◽  
Beta Cell Viability

scholarly journals A Novel Method for the Assessment of Cellular Composition and Beta-Cell Viability in Human Islet Preparations

2005 ◽  
Vol 5 (7) ◽  
pp. 1635-1645 ◽  
Author(s):  
Hirohito Ichii ◽  
Luca Inverardi ◽  
Antonello Pileggi ◽  
R. Damaris Molano ◽  
Over Cabrera ◽  
...  
Keyword(s):  
Beta Cell ◽  
Cell Viability ◽  
Human Islet ◽  
Cellular Composition ◽  
Novel Method ◽  
Beta Cell Viability

scholarly journals Alkannin Inhibits the Development of Ovarian Cancer by Affecting miR-4461

2021 ◽  
Vol 2021 ◽  
pp. 1-8
Author(s):  
Yaowen Wang ◽  
Jingfang Zhang ◽  
Feipeng Wang ◽  
Wenping Chen ◽  
Jie Ma ◽  
...  
Keyword(s):  
Ovarian Cancer ◽  
Flow Cytometry ◽  
Cell Viability ◽  
Migration And Invasion ◽  
Flow Cytometry Analysis ◽  
Antibacterial Effects ◽  
Transwell Assay ◽  
Ovarian Cells ◽  
Mrna And Protein Expression ◽  
Inhibit Cell Proliferation

Background. Previous studies have shown that alkannin has anticancer, anti-inflammatory, and antibacterial effects. However, the effect of alkannin in the development of ovarian cancer (OC) remains unknown. Therefore, this study aims to elucidate the function of alkannin in OC progression. Methods. RT-qPCR and western blot analysis were used to measure mRNA and protein expression. Cell viability and metastasis were detected by the CCK-8 assay, flow cytometry analysis, and transwell assay. Results. Alkannin had no cytotoxicity toward normal ovarian cells, but alkannin can inhibit cell proliferation and induce apoptosis in OC cells. In addition, alkannin inhibited cell migration and invasion and blocked EMT in OC. Besides, upregulation of miR-4461 was found in OC tissues and cells, which was regulated by alkannin. More importantly, miR-4461 can inverse the effects of alkannin on cell viability and metastasis in OC cells. Conclusion. Alkannin restrains cell viability, metastasis, and EMT in OC by downregulating miR-4461 expression.

scholarly journals Cocoa flavonoid epicatechin protects pancreatic beta cell viability and function against oxidative stress

2013 ◽  
Vol 58 (3) ◽  
pp. 447-456 ◽  
Author(s):  
María Ángeles Martín ◽  
Elisa Fernández-Millán ◽  
Sonia Ramos ◽  
Laura Bravo ◽  
Luis Goya
Keyword(s):  
Oxidative Stress ◽  
Beta Cell ◽  
Cell Viability ◽  
Pancreatic Beta Cell ◽  
And Function ◽  
Beta Cell Viability

scholarly journals The transcription factor STAT6 plays a critical role in promoting beta cell viability and is depleted in islets of individuals with type 1 diabetes

Diabetologia ◽  
2018 ◽  
Vol 62 (1) ◽  
pp. 87-98 ◽  
Author(s):  
Kaiyven A. Leslie ◽  
Mark A. Russell ◽  
Kazuto Taniguchi ◽  
Sarah J. Richardson ◽  
Noel G. Morgan
Keyword(s):  
Type 1 Diabetes ◽  
Transcription Factor ◽  
Beta Cell ◽  
Cell Viability ◽  
Critical Role ◽  
Beta Cell Viability

Does Phenytoin Have Neuroprotective Role and Affect Biocompatibility of Decellularized Sciatic Nerve Scaffold?

2020 ◽  
Vol In Press (In Press) ◽  
Author(s):  
Somayyeh Abbaszadeh ◽  
Asadollah Asadi ◽  
Saber Zahri ◽  
Arash Abdolmaleki ◽  
Fariba Mahmoudi
Keyword(s):  
Stem Cells ◽  
Flow Cytometry ◽  
Sciatic Nerve ◽  
Cell Viability ◽  
Mtt Assay ◽  
Good Condition ◽  
Definitive Treatment ◽  
Control Group ◽  
Flow Cytometry Analysis ◽  
Differentiation Potential

Background: Peripheral nervous system injuries are common and currently have no definitive treatment method. Phenytoin is one of the main antiepileptic drugs. Some studies have described a cerebroprotective effect of phenytoin in an established model of global cerebral ischemia. Objectives: In this study, the neuroprotective effects of phenytoin were evaluated on the cultivation and maintenance of Wharton’s jelly stem cells (WJSCs) on acellularized sciatic nerve scaffolds. Methods: In this study, acellular scaffolds from the rat sciatic nerve were prepared by the sondell method. After extraction of cells of MSCs, flow cytometry analysis was executed. Also, cell differentiation potential was evaluated by placement in osteogenic and adipogenic differentiation media for 21 days. Biocompatibility of the scaffold and cell viability were investigated using the MTT assay. The morphological and cell adhesion characteristics of MSCs on acellular scaffolds were compared using SEM micrographs images. Data were analyzed using the one-way analysis of variance (ANOVA) and Tukey post hoc test by SPSS (version 19.0) software. Results: The removal of cells from the scaffold was confirmed by stanning with hematoxylin-eosin, van Gieson's picro-fuchsin, and DAPI. With the aid of flow cytometry analysis and differentiation into bone and fat cells, it was confirmed that extracted cells were mesenchymal stem cells. The results of the MTT assay showed that phenytoin increased cell viability and retention on the scaffold. Conclusions: The study indicated that phenytoin improves the viability of cells and provided a good condition for the growth, survival, and attachment of cells to the scaffold when compared to the control group. These results suggest that phenytoin can be considered a new treatment for nerve regeneration and tissue engineering applications.

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