scholarly journals Use of oral fluids for efficient monitoring of influenza viruses in swine herds in Colombia

2021 ◽  
Author(s):  
Karl A Ciuoderis ◽  
Laura S Perez ◽  
Andrés Cardona ◽  
Juan Pablo Hernandez-Ortíz ◽  
Jorge E Osorio
Keyword(s):  
Influenza Viruses ◽  
Oral Fluids ◽  
Swine Herds

scholarly journals Pilot Investigation of Anti-Salmonella Antibodies in Oral Fluids from Salmonella Typhimurium Vaccinated and Unvaccinated Swine Herds

Animals ◽  
2021 ◽  
Vol 11 (8) ◽  
pp. 2408
Author(s):  
Alessia De Lucia ◽  
Shaun A. Cawthraw ◽  
Richard Piers Smith ◽  
Rob Davies ◽  
Carlo Bianco ◽  
...  
Keyword(s):  
Salmonella Typhimurium ◽  
Oral Fluid ◽  
Igg Antibodies ◽  
Live Attenuated Vaccine ◽  
Non Invasive ◽  
Meat Juice ◽  
Oral Fluids ◽  
Faecal Samples ◽  
Pilot Investigation ◽  
Swine Herds

Oral fluid (OF) can be a simple, cheap and non-invasive alternative to serum or meat juice for the diagnosis and surveillance of important pathogens in pigs. This study was conducted on four Salmonella Typhimurium-positive farrow-to-finish pig farms: two Salmonella-vaccinated (V) and two non-vaccinated (NV). Gilts and sows in the V farms were vaccinated with a live, attenuated vaccine prior to farrowing. Pooled faecal and OF samples were collected from the sows and their offspring. Salmonella was isolated according to ISO6579–1:2017. In parallel, IgG and IgA levels were assessed in OF samples using a commercial ELISA assay. Salmonella was detected in 90.9% of the pooled faecal samples from the NV farms and in 35.1% of the pooled faecal samples from the V farms. Overall, a higher prevalence was observed in the pooled faecal samples from the offspring (76.3%) compared to the sows (36.4%). IgG antibodies measured in V farms are likely to be related to vaccination, as well as exposure to Salmonella field strains. The detection of IgA antibodies in OF was unreliable with the method used. The results of this study show that IgG is the most reliable isotype for monitoring Salmonella-specific antibody immunity in vaccinated/infected animals via OF.

Isolation and complete genomic characterization of pandemic H1N1/2009 influenza viruses from Cuban swine herds

2013 ◽  
Vol 94 (3) ◽  
pp. 781-788 ◽  
Author(s):  
Lester Josué Pérez ◽  
Carmen Laura Perera ◽  
Armando Vega ◽  
Maria T. Frías ◽  
Dagmar Rouseaux ◽  
...  
Keyword(s):  
Influenza Viruses ◽  
Pandemic H1n1 ◽  
Genomic Characterization ◽  
Pandemic H1n1 2009 ◽  
Swine Herds ◽  
Complete Genomic

scholarly journals Isolation of H3N2 Swine Influenza Virus in South Korea

2003 ◽  
Vol 15 (1) ◽  
pp. 30-34 ◽  
Author(s):  
Dae S. Song ◽  
Jae Y. Lee ◽  
Jin S. Oh ◽  
Kwang S. Lyoo ◽  
Kyung J. Yoon ◽  
...  
Keyword(s):  
Influenza Virus ◽  
South Korea ◽  
Respiratory Disease ◽  
Swine Influenza Virus ◽  
Swine Influenza ◽  
Influenza Viruses ◽  
H3n2 Virus ◽  
Pcr Products ◽  
Swine Population ◽  
Swine Herds

Swine influenza is a significant respiratory disease causing occasional reproductive problems in nïve swine herds. Although different subtypes of swine influenza virus (SIV) have been implicated in clinical outbreaks of swine influenza in Asian countries, no virus isolation has been made to identify SIV of subtypes other than the H1N1 subtype in the Korean swine population. In December 1998, an outbreak of acute respiratory disease was identified in a commercial swine farm located in the Kyunggi province of South Korea. A causative agent, which agglutinated rooster red blood cells, was detected from the lungs of 3 piglets from the index herd and was determined to be type A influenza virus using a commercial influenza virus typing kit. Hemagglutination activity (HA) of the isolates was completely inhibited by a swine antiserum against a recent US H3N2 SIV isolate (A/Sw/IA/41305/1998) but not by H1N1 swine antiserum (A/Sw/IA/1979). Reverse transcription–polymerase chain reaction (RT-PCR) revealed all 3 isolates were H3 SIV subtypes. Sequence analysis of hemagglutinin gene PCR products supported the belief that the Korean H3 SIV isolates were genetically similar to the known mammalian H3 influenza viruses. This is the first report on a clinical outbreak of swine influenza caused by the H3N2 virus in Korea.

scholarly journals Pathogenicity and Transmissibility of Novel Reassortant H3N2 Influenza Viruses with 2009 Pandemic H1N1 Genes in Pigs

2014 ◽  
Vol 89 (5) ◽  
pp. 2831-2841 ◽  
Author(s):  
Jingjiao Ma ◽  
Huigang Shen ◽  
Qinfang Liu ◽  
Bhupinder Bawa ◽  
Wenbao Qi ◽  
...  
Keyword(s):  
Binding Site ◽  
Receptor Binding ◽  
The United States ◽  
Influenza Viruses ◽  
H3n2 Virus ◽  
Pandemic H1n1 ◽  
M Gene ◽  
Receptor Binding Site ◽  
H3n2 Influenza ◽  
Swine Herds

ABSTRACTAt least 10 different genotypes of novel reassortant H3N2 influenza viruses with 2009 pandemic H1N1 [A(H1N1)pdm09] gene(s) have been identified in U.S. pigs, including the H3N2 variant with a single A(H1N1)pdm09 M gene, which has infected more than 300 people. To date, only three genotypes of these viruses have been evaluated in animal models, and the pathogenicity and transmissibility of the other seven genotype viruses remain unknown. Here, we show that three H3N2 reassortant viruses that contain 3 (NP, M, and NS) or 5 (PA, PB2, NP, M, and NS) genes from A(H1N1)pdm09 were pathogenic in pigs, similar to the endemic H3N2 swine virus. However, the reassortant H3N2 virus with 3 A(H1N1)pdm09 genes and a recent human influenza virus N2 gene was transmitted most efficiently among pigs, whereas the reassortant H3N2 virus with 5 A(H1N1)pdm09 genes was transmitted less efficiently than the endemic H3N2 virus. Interestingly, the polymerase complex of reassortant H3N2 virus with 5 A(H1N1)pdm09 genes showed significantly higher polymerase activity than those of endemic and reassortant H3N2 viruses with 3 A(H1N1)pdm09 genes. Further studies showed that an avian-like glycine at position 228 at the hemagglutinin (HA) receptor binding site is responsible for inefficient transmission of the reassortant H3N2 virus with 5 A(H1N1)pdm09 genes. Taken together, our results provide insights into the pathogenicity and transmissibility of novel reassortant H3N2 viruses in pigs and suggest that a mammalian-like serine at position 228 in the HA is critical for the transmissibility of these reassortant H3N2 viruses.IMPORTANCESwine influenza is a highly contagious zoonotic disease that threatens animal and public health. Introduction of 2009 pandemic H1N1 virus [A(H1N1)pdm09] into swine herds has resulted in novel reassortant influenza viruses in swine, including H3N2 and H1N2 variants that have caused human infections in the United States. We showed that reassortant H3N2 influenza viruses with 3 or 5 genes from A(H1N1)pdm09 isolated from diseased pigs are pathogenic and transmissible in pigs, but the reassortant H3N2 virus with 5 A(H1N1)pdm09 genes displayed less efficient transmissibility than the endemic and reassortant H3N2 viruses with 3 A(H1N1)pdm09 genes. Further studies revealed that an avian-like glycine at the HA 228 receptor binding site of the reassortant H3N2 virus with 5 A(H1N1)pdm09 genes is responsible for less efficient transmissibility in pigs. Our results provide insights into viral pathogenesis and the transmission of novel reassortant H3N2 viruses that are circulating in U.S. swine herds and warrant future surveillance.

Emergence and characterisation of pandemic H1N1 influenza viruses in Hungarian swine herds

2013 ◽  
Vol 61 (1) ◽  
pp. 125-134 ◽  
Author(s):  
Ádám Bálint ◽  
István Kiss ◽  
Krisztián Bányai ◽  
Imre Biksi ◽  
Katalin Szentpáli-Gavallér ◽  
...  
Keyword(s):  
Influenza Virus ◽  
Amino Acid ◽  
H1n1 Influenza ◽  
Influenza Viruses ◽  
Viral Fitness ◽  
Pandemic H1n1 ◽  
H1n1 Influenza Virus ◽  
Pandemic H1n1 Influenza ◽  
Molecular Features ◽  
Swine Herds

In 2010, two novel porcine H1N1 influenza viruses were isolated from pigs with influenza-like illness in Hungarian swine herds. Sequence and phylogenetic analysis of these strains revealed that they shared molecular features with the pandemic H1N1 influenza virus strains, which emerged globally during 2009. The PB2, HA and NA genes contained unique amino acid changes compared to the available new H1N1 influenza virus sequences of pig origin. Furthermore, the investigated strains could be separated with respect to parallel amino acid substitutions affecting the polymerase genes (PB2, PB1 and PA) and the nucleoprotein (NP) gene, supporting the proposed complementarities between these proteins, all required for the viral fitness. Molecular characterisation of two Hungarian human pandemic H1N1 isolates was also performed, so that we could compare contemporaneous strains of different host species origins. Shared molecular motifs in various genes of animal and human influenza strains suggested that the Hungarian porcine strains could have originated from humans through direct interspecies transmission. This study is among the few that support the natural human-to-pig transmission of the pandemic H1N1 influenza virus.

Seroprevalence and genetic evolutions of swine influenza viruses under vaccination pressure in Korean swine herds

2008 ◽  
Vol 138 (1-2) ◽  
pp. 43-49 ◽  
Author(s):  
Philippe Noriel Q. Pascua ◽  
Min-Suk Song ◽  
Jun Han Lee ◽  
Hwan-Woon Choi ◽  
Jeong Hee Han ◽  
...  
Keyword(s):  
Swine Influenza ◽  
Influenza Viruses ◽  
Swine Herds

Rapid detection of Chlamydia/Chlamydophila group in samples collected from swine herds with and without reproductive disorders

2014 ◽  
Vol 17 (2) ◽  
pp. 367-369 ◽  
Author(s):  
K. Rypula ◽  
A. Kumala ◽  
P. Lis ◽  
K. Niemczuk ◽  
K. Płoneczka-Janeczko ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Rapid Detection ◽  
Complement Fixation Test ◽  
Complement Fixation ◽  
Fixation Test ◽  
Reproductive Disorders ◽  
Serum Samples ◽  
Swine Herds

Abstract The study was carried out in seven reproductive herds of pigs. In three of them reproductive disorders were observed. Three herds consisted of 10-50 and four consisted of 120-500 adult sows and they were called small and medium, respectively. Fifty-seven adult sows were randomly selected from herds. Serum samples were tested using the complement fixation test and swabs from both eyes and from the vaginal vestibule were examined using real-time PCR. All serum samples were negative. Infected sows were present in each of the study herds. In total, there were 28 positive samples (53%, 28/48) in real-time PCR in sows with reproductive disorders and 35 (53%, 35/66) in sows selected from herds without problems in reproduction. One isolate proved to be Chlamydophila pecorum, whereas all the remaining were Chamydia suis

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