Protective Effects of Zingiber zerumbet Ethyl Acetate Extract on Hydrogen Peroxide-Induced Damage of Red Blood Cells

The synthesis and antioxidant of some new pyrazole analogs were described. It is achieved by the reaction of phenyl-4-(phenylsulfonyl)-1H-pyrazole-3,5-diamine (3) with different bifunctional reagents. The free radical-induced damage and the protective effects of the newly synthesized pyrazoles were studied. These new compounds inhibit the free radical-induced oxidative hemolysis of red blood cells effectively. It was found that these compounds effectively inhibit the free radical-induced oxidative hemolysis of red blood cells. Compound 5, which contain phenolic group, and 17, which bear sulfur, nitrogen atoms, and benzothiazole ring, respectively displayed high antioxidant activity. Analogs 15, 11, 10, and 9 were proved to exhibit antioxidative activity. Structures of new pyrazoles were confirmed by spectroscopic and elemental analyses and have been screened for their antioxidant activity.

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Protective Effects of Resveratrol and its Analogues against Free Radical-Induced Oxidative Hemolysis of Red Blood Cells†

Chinese Journal of Chemistry ◽

10.1002/cjoc.20020201126 ◽

2010 ◽

Vol 20 (11) ◽

pp. 1313-1318 ◽

Cited By ~ 4

Author(s):

Jian-Guo Fang ◽

Man Lu ◽

Lan-Ping Ma ◽

Li Yang ◽

Long-Min Wu ◽

...

Keyword(s):

Free Radical ◽

Red Blood Cells ◽

Blood Cells ◽

Protective Effects ◽

Oxidative Hemolysis

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Contrasting effects of stored allogeneic red blood cells and their supernatants on permeability and inflammatory responses in human pulmonary endothelial cells

AJP Lung Cellular and Molecular Physiology ◽

10.1152/ajplung.00025.2019 ◽

2020 ◽

Vol 318 (3) ◽

pp. L533-L548

Author(s):

Junghyun Kim ◽

Trang T. T. Nguyen ◽

Yue Li ◽

Chen-Ou Zhang ◽

Boyoung Cha ◽

...

Keyword(s):

Red Blood Cells ◽

Blood Cells ◽

Map Kinases ◽

Inflammatory Responses ◽

Intercellular Junctions ◽

Protective Effects ◽

Pyrin Domain ◽

Inflammatory Conditions ◽

Tnf Α ◽

Rbc Transfusion

Transfusion of red blood cells (RBCs) is a common life-saving clinical practice in severely anemic or hemorrhagic patients; however, it may result in serious pathological complications such as transfusion-related acute lung injury. The factors mediating the deleterious effects of RBC transfusion remain unclear. In this study, we tested the effects of washed long-term (RBC-O; >28 days) versus short-term (RBC-F; <14 days) stored RBCs and their supernatants on lung endothelial (EC) permeability under control and inflammatory conditions. RBCs enhanced basal EC barrier function as evidenced by an increase in transendothelial electrical resistance and decrease in permeability for macromolecules. RBCs also attenuated EC hyperpermeability and suppressed secretion of EC adhesion molecule ICAM-1 and proinflammatory cytokine IL-8 in response to LPS or TNF-α. In both settings, RBC-F had slightly higher barrier protective effects as compared with RBC-O. In contrast, supernatants from both RBC-F and RBC-O disrupted the EC barrier. The early phase of EC permeability response caused by RBC supernatants was partially suppressed by antioxidant N-acetyl cysteine and inhibitor of Src kinase family PP2, while addition of heme blocker and inhibition of NOD-like receptor family pyrin domain containing protein 3 (NLRP3), stress MAP kinases, receptor for advanced glycation end-products (RAGE), or Toll-like receptor-4 (TLR4) signaling were without effect. Morphological analysis revealed that RBC supernatants increased LPS- and TNF-α-induced breakdown of intercellular junctions and formation of paracellular gaps. RBC supernatants augmented LPS- and TNF-α-induced EC inflammation reflected by increased production of IL-6, IL-8, and soluble ICAM-1. These findings demonstrate the deleterious effects of RBC supernatants on EC function, which may have a major impact in pathological consequences associated with RBC transfusion.

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In Vitro Assessment of Antiplasmodial Activity and Cytotoxicity of Polyalthia longifolia Leaf Extracts on Plasmodium falciparum Strain NF54

Malaria Research and Treatment ◽

10.1155/2019/6976298 ◽

2019 ◽

Vol 2019 ◽

pp. 1-9 ◽

Cited By ~ 2

Author(s):

Bethel Kwansa-Bentum ◽

Kojo Agyeman ◽

Jeffrey Larbi-Akor ◽

Claudia Anyigba ◽

Regina Appiah-Opong

Keyword(s):

Plasmodium Falciparum ◽

Ethyl Acetate ◽

Red Blood Cells ◽

Blood Cells ◽

Radical Scavenging ◽

Antimalarial Drugs ◽

Antiplasmodial Activity ◽

Leaf Extracts ◽

Polyalthia Longifolia

Background. Malaria is one of the most important life-threatening infectious diseases in the tropics. In spite of the effectiveness of artemisinin-based combination therapy, reports on reduced sensitivity of the parasite to artemisinin in Cambodia and Thailand warrants screening for new potential antimalarial drugs for future use. Ghanaian herbalists claim that Polyalthia longifolia has antimalarial activity. Therefore, antiplasmodial activity, cytotoxic effects, and antioxidant and phytochemical properties of P. longifolia leaf extract were investigated in this study. Methodology/Principal Findings. Aqueous, 70% hydroethanolic and ethyl acetate leaf extracts were prepared using standard procedures. Antiplasmodial activity was assessed in vitro by using chloroquine-sensitive malaria parasite strain NF54. The SYBR® Green and tetrazolium-based calorimetric assays were used to measure parasite growth inhibition and cytotoxicity, respectively, after extract treatment. Total antioxidant activity was evaluated using a free radical scavenging assay. Results obtained showed that extracts protected red blood cells against Plasmodium falciparum mediated damage. Fifty percent inhibitory concentration (IC50) values were 24.0±1.08 μg/ml, 22.5±0.12 μg/ml, and 9.5±0.69 μg/ml for aqueous, hydroethanolic, and ethyl acetate extracts, respectively. Flavonoids, tannins, and saponins were present in the hydroethanolic extract, whereas only the latter was observed in the aqueous extract. Aqueous and hydroethanolic extracts showed stronger antioxidant activities compared to the ethyl acetate extract. Conclusions/Significance. The extracts of P. longifolia have antiplasmodial properties and low toxicities to human red blood cells. The extracts could be developed as useful alternatives to antimalarial drugs. These results support claims of the herbalists that decoctions of P. longifolia are useful antimalarial agents.

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An LC–MS Assay to Measure Superoxide Radicals and Hydrogen Peroxide in the Blood System

Metabolites ◽

10.3390/metabo10050175 ◽

2020 ◽

Vol 10 (5) ◽

pp. 175 ◽

Cited By ~ 1

Author(s):

Ioannis Tsamesidis ◽

Chinedu O. Egwu ◽

Pierre Pério ◽

Jean-Michel Augereau ◽

Françoise Benoit-Vical ◽

...

Keyword(s):

Mass Spectrometry ◽

Hydrogen Peroxide ◽

Red Blood Cells ◽

Blood Cells ◽

High Resolution Mass Spectrometry ◽

Modern Medicine ◽

Reactive Species ◽

Blood System ◽

Pathological Conditions ◽

Depth Study

Red blood cells are constantly exposed to reactive species under physiological or pathological conditions or during administration of xenobiotics. Regardless of the source, its accurate quantification is paramount in the area of theragnostics, which had been elusive up until now. Even if there are a lot of approaches to evaluate the oxidative stress, very sensitive methods are missing for the blood system. We therefore sought to apply a highly sensitive approach, by liquid chromatography coupled to mass spectrometry (UPLC–MS), for the quantification of reactive species such as superoxide radical and hydrogen peroxide using dihydroethidium (DHE) and coumarin boronic acid (CBA) probes respectively through the detection of 2-hydroxyethidium (2OH-E+) and 7-hydroxycoumarin (COH). The use of the high-resolution mass spectrometry associated to UPLC ensured a selective detection of superoxide and hydrogen peroxide in the blood system under diverse conditions such as oxidized red blood cells (RBCs), untreated and treated parasitized RBCs. Moreover, this technique allowed the determination of reactive species in human plasma. This protocol provides a huge opportunity for in-depth study of several pathological conditions vis-a-vis their treatment in modern medicine.

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