Hidrogel Berasaskan Pektin Kulit Buah Naga (Hylocereus  polyrhizus) sebagai Pembawa Ubat melalui Sistem Penghantar Oral

Azwan Mat Lazim; Farahain Mokhtar; Kalaivani Sanger; A. Azfaralariff; Izwan Ishak; I. Faiz Mustafa; S.I. Zubairi; Shazrul Fazry; Rizafizah Othaman; Mazlan Mohamed; Norhakimin Abdullah; H.H. Hamzah

doi:10.17576/jsm-2021-5011-10

Hidrogel Berasaskan Pektin Kulit Buah Naga (Hylocereus polyrhizus) sebagai Pembawa Ubat melalui Sistem Penghantar Oral

Sains Malaysiana ◽

10.17576/jsm-2021-5011-10 ◽

2021 ◽

Vol 50 (11) ◽

pp. 3263-3273

Author(s):

Azwan Mat Lazim ◽

Farahain Mokhtar ◽

Kalaivani Sanger ◽

A. Azfaralariff ◽

Izwan Ishak ◽

...

Keyword(s):

Danio Rerio ◽

Hylocereus Polyrhizus

Kajian ini memfokuskan kepada aplikasi hidrogel berasaskan ekstrak pektin daripada kulit buah naga (Hylocereus polyrhizus) yang digunakan sebagai biobahan pembawa ubat. Sebanyak 5 sampel telah disediakan dengan peratus komposisi pektin yang berbeza (0%, 0.5%, 1.0%, 1.5%, 2.0%). Keputusan menunjukkan hidrogel dengan 1.0% pektin telah mengembang secara maksimum pada pH 7. Imej daripada mikroskop elektron pengimbas (SEM) mendapati saiz keporosan hidrogel meningkat dengan penambahan pektin. Potensi hidrogel sebagai agen pembawa ubat telah dilakukan dengan melihat kecekapan muatan ubat, ujian perlepasan ubat dan ujian biodegradasi di dalam saluran pencernaan menggunakan medium simulasi perut (SGF), usus (SIF) dan kolon (SCF) secara in-vitro dengan kehadiran enzim. Hasil ujian ketoksikan menggunakan kaedah embrio ikan kuda belang (Danio rerio) telah memberikan keputusan yang negatif terhadap hidrogel yang diuji.

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Oocyte Ageing in Zebrafish Danio rerio (Hamilton, 1822) and Its Consequence on the Viability and Ploidy Anomalies in the Progeny

Animals ◽

10.3390/ani11030912 ◽

2021 ◽

Vol 11 (3) ◽

pp. 912

Author(s):

Swapnil Gorakh Waghmare ◽

Azadeh Mohagheghi Samarin ◽

Roman Franěk ◽

Martin Pšenička ◽

Tomáš Policar ◽

...

Keyword(s):

Danio Rerio ◽

Egg Quality ◽

Complete Loss ◽

Egg Viability ◽

Embryo Survival ◽

Hatching Rates

Fish egg quality can be markedly influenced by the oocyte age after ovulation. In this study, we examined the duration of oocyte ageing in the zebrafish (Danio rerio) and whether prolonged ageing is associated with the incidence of ploidy anomalies in the resulting embryos. Oocytes were incubated in vitro for 6 h post-stripping (HPS) at 26 °C and fertilized at 2-h intervals. Meanwhile, for eggs fertilized immediately after stripping, the fertilization, embryo survival, and hatching rates started at ~80%; these rates decreased to 39%, 24%, and 16%, respectively, for oocytes that had been stored for 4 h (p ˂ 0.05), and there was an almost complete loss of egg viability at 6 HPS. Furthermore, almost 90% of the embryos derived from 6-h aged oocytes died prior to hatching, and all larvae originating from 4- and 6-h aged oocytes showed malformations. The proportion of ploidy abnormal embryos was significantly greater at 4 HPS (18.5%) than at either 0 or 2 HPS (4.7% and 8.8%, respectively). The results revealed that zebrafish oocytes retained their fertilization potential for up to 2 h after stripping at 26 °C and indicated the contribution of post-ovulatory oocyte ageing in the occurrence of ploidy anomalies in the resulting embryos.

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The influence of the fortification of red pitaya ( Hylocereus polyrhizus ) powder on the in vitro digestion, physical parameters, nutritional profile, polyphenols, and antioxidant activity in the oat‐wheat bread

International Journal of Food Science & Technology ◽

10.1111/ijfs.15530 ◽

2021 ◽

Author(s):

Yanyi Huang ◽

Mengya He ◽

Stefan Kasapis ◽

Margaret Brennan ◽

Charles Brennan

Keyword(s):

Antioxidant Activity ◽

In Vitro Digestion ◽

Wheat Bread ◽

Physical Parameters ◽

Nutritional Profile ◽

Hylocereus Polyrhizus

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A characterization of the ZFL cell line and primary hepatocytes as in vitro liver cell models for the zebrafish (Danio rerio)

Aquatic Toxicology ◽

10.1016/j.aquatox.2013.11.023 ◽

2014 ◽

Vol 147 ◽

pp. 7-17 ◽

Cited By ~ 28

Author(s):

Marta Eide ◽

Marte Rusten ◽

Rune Male ◽

Knut Helge Midtbø Jensen ◽

Anders Goksøyr

Keyword(s):

Cell Line ◽

Danio Rerio ◽

Liver Cell ◽

Cell Models ◽

Primary Hepatocytes

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Pemanfaatan Jamur Trichoderma sp Sebagai Antagonis Patogen Busuk Sulur Tanaman Buah Naga Merah (Hylocereus polyrhizus) Secara In Vitro

Agrifarm : Jurnal Ilmu Pertanian ◽

10.24903/ajip.v7i1.367 ◽

2018 ◽

Vol 7 (1) ◽

pp. 19

Author(s):

Herlyan Prasetiyo ◽

Purwati Purwati ◽

Iin Arsensi

Keyword(s):

Plant Disease ◽

Secondary Data ◽

Pathogenic Fungi ◽

Primary Data ◽

Field Observations ◽

Trichoderma Sp ◽

Dragon Fruit ◽

Fruit Plants ◽

Hylocereus Polyrhizus

Utilization of Trichoderma sp fungi as pathogenic fungi antagonists in red dragon fruit plants (Hylocereus polyrhizus) in vitro. The purpose of this study was to identify foul pathogens of dragon fruit plants and then test the ability of Trichoderma sp antagonists to deciduous pathogens of red dragon fruit plants in vitro. The study was conducted from August to October 2016, The research was conducted at the Laboratory of Pest and Plant Disease Sciences, Faculty of Agriculture, Mulawarman University, Samarinda. Sampling of plants exposed to foul tendrils was carried out in Bukit Merdeka Village, Samboja District, Kutai Kartanegara Regency. There are two data observed in this study, primary data and secondary data. Primary data is data obtained directly from the source through direct field observations and laboratory observations and secondary data data obtained from interviews with farmers. The results showed that the pathogen that causes tendon rot in dragon fruit plants is the fungus Colletotrichum gloesporioides (penz. Ssaac). Trichoderma sp can inhibit the development of pathogens Colletotrichum gloesporioides (Penz.) Ssaac., With the highest average resistance of 71.85%.

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In vitro cytotoxic and teratogenic potential of sediment extracts from Skadar Lake using fish cell line RTL-W1 and Danio rerio embryos

Archives of Biological Sciences ◽

10.2298/abs1304539p ◽

2013 ◽

Vol 65 (4) ◽

pp. 1539-1546 ◽

Cited By ~ 3

Author(s):

Andrej Perovic ◽

Svetlana Perovic ◽

Thomas-Benjamin Seiler ◽

Henner Hollert

Keyword(s):

Cell Line ◽

Danio Rerio ◽

Fish Cell Line ◽

Fish Cell ◽

Teratogenic Potential ◽

Danio Rerio Embryos

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POTENCIAL ANTIBACTERIANO IN VITRO E TOXICOLÓGICO IN VIVO DAS FOLHAS DO JAMBO VERMELHO (Syzygium malaccensis, Myrtaceae) FRENTE A ZEBRAFISH (D. rerio) ADULTO

10.53934/9786599539626.26 ◽

2021 ◽

Author(s):

Fernando E. T. Cunha ◽

Maria I. C. Ferreira ◽

Rafael S. Cruz ◽

Maria J. G. Ferreira ◽

Clarissa M. Aquino ◽

...

Keyword(s):

Escherichia Coli ◽

Staphylococcus Aureus ◽

Listeria Monocytogenes ◽

Danio Rerio ◽

Salmonella Enteritidis

Este trabalho reporta o potencial antibacteriano in vitro e toxicológico in vivo das folhas do jambo (Syzygium malaccense) frente a zebrafish (Danio rerio) adulto (ZFa). As folhas de jambo foram submetidas a desidratação (35 ± 2°C) por 24 horas, trituração e posterior extração de metabólitos por decocção, infusão e maceração com água destilada. Os extratos obtidos foram liofilizados e submetidos a análise de atividade antibacteriana in vitro frente a Gram-negativas (Escherichia coli ATCC 25922, Salmonella Enteritidis IAL 1132) e Gram-positivas (Listeria monocytogenes ATCC 19115 e Staphylococcus aureus ATCC 27664), bem como ao potencial toxicológico in vivo frente ao ZFa. O extrato obtido por infusão se mostrou mais promissor, pois apresentou concentração mínima bactericida (CMB) e concentração mínima inibitória (CMI) com maior potencial frente às gram- positivas (CMB - 6,25 e CMI - 6,25 mg/ml), bem como às gram-negativas (CMB - 25,0 e 3,125 e CMI - 3,125 mg/ml). Todos os extratos testados não se mostraram tóxicos frente ao zebrafish adulto e não alteraram o sistema locomotor dos mesmos. Desta forma, conclui-se que o extrato aquoso das folhas do jambo vermelho (Syzygium malaccense) obtido por infusão é seguro e pode ser utilizado como conservante natural com maior ação antibacteriana. Este trabalho nos conduz a novos estudos de isolamento e caracterização de princípios bioativos.

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