Biofilm Formation and Bacterial Aggregation Response of Planomicrobium chinense and Alkaligenes faecalis Associated with Periplaneta americana Found in Household Sewerage

ABSTRACT Various bacterial pathogens display an intracellular lifestyle and spread from cell to cell through actin-based motility (ABM). ABM requires actin polymerization at the bacterial pole and is mediated by the expression of bacterial factors that hijack the host cell actin nucleation machinery or exhibit intrinsic actin nucleation properties. It is increasingly recognized that bacterial ABM factors, in addition to having a crucial task during the intracellular phase of infection, display “moonlighting” adhesin functions, such as bacterial aggregation, biofilm formation, and host cell adhesion/invasion. Here, we review our current knowledge of ABM factors and their additional functions, and we propose that intracellular ABM functions have evolved from ancestral, extracellular adhesin functions.

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Influence of Calcium in Extracellular DNA Mediated Bacterial Aggregation and Biofilm Formation

PLoS ONE ◽

10.1371/journal.pone.0091935 ◽

2014 ◽

Vol 9 (3) ◽

pp. e91935 ◽

Cited By ~ 66

Author(s):

Theerthankar Das ◽

Shama Sehar ◽

Leena Koop ◽

Yie Kuan Wong ◽

Safia Ahmed ◽

...

Keyword(s):

Biofilm Formation ◽

Extracellular Dna ◽

Bacterial Aggregation

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Bundle-Forming Pili and EspA Are Involved in Biofilm Formation by Enteropathogenic Escherichia coli

Journal of Bacteriology ◽

10.1128/jb.00177-06 ◽

2006 ◽

Vol 188 (11) ◽

pp. 3952-3961 ◽

Cited By ~ 53

Author(s):

Cristiano G. Moreira ◽

Kelli Palmer ◽

Marvin Whiteley ◽

Marcelo P. Sircili ◽

Luiz R. Trabulsi ◽

...

Keyword(s):

Escherichia Coli ◽

Biofilm Formation ◽

Signaling Mechanism ◽

Bacterial Aggregation ◽

Abiotic Surfaces ◽

Genes Encoding ◽

Continuous Culture System ◽

Static Conditions ◽

Biofilm Development ◽

Isogenic Mutants

ABSTRACT Microcolony formation is one of the initial steps in biofilm development, and in enteropathogenic Escherichia coli (EPEC) it is mediated by several adhesins, including the bundle-forming pilus (BFP) and the EspA filament. Here we report that EPEC forms biofilms on plastic under static conditions and a flowthrough continuous culture system. The abilities of several EPEC isogenic mutants to form biofilms were assessed. Adhesins such as BFP and EspA, important in microcolony formation on epithelial cells, are also involved in bacterial aggregation during biofilm formation on abiotic surfaces. Mutants that do not express BFP or EspA form more-diffuse biofilms than does the wild type. We also determined, using gfp transcriptional fusions, that, consistent with the role of these adhesins in biofilms, the genes encoding BFP and EspA are expressed during biofilm formation. Finally, expression of espA is controlled by a quorum-sensing (QS) regulatory mechanism, and the EPEC qseA QS mutant also forms altered biofilms, suggesting that this signaling mechanism plays an important role in EPEC biofilm development. Taken together, these studies allowed us to propose a model of EPEC biofilm formation.

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Characteristics of Biofilm Formation by Streptococcus mutans in the Presence of Saliva

Infection and Immunity ◽

10.1128/iai.00422-08 ◽

2008 ◽

Vol 76 (9) ◽

pp. 4259-4268 ◽

Cited By ~ 87

Author(s):

Sug-Joon Ahn ◽

Sang-Joon Ahn ◽

Zezhang T. Wen ◽

L. Jeannine Brady ◽

Robert A. Burne

Keyword(s):

Streptococcus Mutans ◽

Parental Strain ◽

Biofilm Formation ◽

Fluid Phase ◽

Trigger Factor ◽

Bacterial Aggregation ◽

Whole Saliva ◽

Tooth Surfaces ◽

Biofilm Development ◽

Induced Aggregation

ABSTRACT Interactions between salivary agglutinin and the adhesin P1 of Streptococcus mutans contribute to bacterial aggregation and mediate sucrose-independent adherence to tooth surfaces. We have examined biofilm formation by S. mutans UA159, and derivative strains carrying mutations affecting the localization or expression of P1, in the presence of fluid-phase or adsorbed saliva or salivary agglutinin preparations. Whole saliva- and salivary agglutinin-induced aggregation of S. mutans was adversely affected by the loss of P1 and sortase (SrtA) but not by the loss of trigger factor (RopA). Fluid-phase salivary agglutinin and, to a lesser extent, immobilized agglutinin inhibited biofilm development by S. mutans in the absence of sucrose, and whole saliva was more effective at decreasing biofilm formation than salivary agglutinin. Inhibition of biofilm development by salivary agglutinin was differently influenced by particular mutations, with the P1-deficient strain displaying a greater inhibition of biofilm development than the SrtA- or RopA-deficient strains. As expected, biofilm-forming capacities of all strains in the presence of salivary preparations were markedly enhanced in the presence of sucrose, although biofilm formation by the mutants was less efficient than that by the parental strain. Aeration strongly inhibited biofilm development, and the presence of salivary components did not restore biofilm formation in aerated conditions. The results disclose a potent ability of salivary constituents to moderate biofilm formation by S. mutans through P1-dependent and P1-independent pathways.

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Biofilm Formation on Dental Implant Biomaterials by Staphylococcus aureus Strains Isolated from Patients with Cystic Fibrosis

Materials ◽

10.3390/ma14082030 ◽

2021 ◽

Vol 14 (8) ◽

pp. 2030

Author(s):

Anna Minkiewicz-Zochniak ◽

Sylwia Jarzynka ◽

Agnieszka Iwańska ◽

Kamila Strom ◽

Bartłomiej Iwańczyk ◽

...

Keyword(s):

Cystic Fibrosis ◽

Staphylococcus Aureus ◽

Biofilm Formation ◽

Bacterial Biofilm ◽

Chromium Alloy ◽

Systemic Diseases ◽

Cobalt Chromium ◽

Bacterial Aggregation ◽

Cobalt Chromium Alloy

Implants made of ceramic and metallic elements, which are used in dentistry, may either promote or hinder the colonization and adhesion of bacteria to the surface of the biomaterial to varying degrees. The increased interest in the use of dental implants, especially in patients with chronic systemic diseases such as cystic fibrosis (CF), is caused by an increase in disease complications. In this study, we evaluated the differences in the in vitro biofilm formation on the surface of biomaterials commonly used in dentistry (Ti-6Al-4V, cobalt-chromium alloy (CoCr), and zirconia) by Staphylococcus aureus isolated from patients with CF. We demonstrated that S. aureus adherence and growth depends on the type of material used and its surface topography. Weaker bacterial biofilm formation was observed on zirconia surfaces compared to titanium and cobalt-chromium alloy surfaces. Moreover, scanning electron microscopy showed clear differences in bacterial aggregation, depending on the type of biomaterial used. Over the past several decades, S. aureus strains have developed several mechanisms of resistance, especially in patients on chronic antibiotic treatment such as CF. Therefore, the selection of an appropriate implant biomaterial with limited microorganism adhesion characteristics can affect the occurrence and progression of oral cavity infections, particularly in patients with chronic systemic diseases.

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The Haemophilus Cryptic Genospecies Cha Adhesin Has at Least Two Variants That Differ in Host Cell Binding, Bacterial Aggregation, and Biofilm Formation Properties

Journal of Bacteriology ◽

10.1128/jb.01409-13 ◽

2014 ◽

Vol 196 (9) ◽

pp. 1780-1788 ◽

Cited By ~ 3

Author(s):

J. R. McCann ◽

A. J. Sheets ◽

S. Grass ◽

J. W. St. Geme

Keyword(s):

Host Cell ◽

Biofilm Formation ◽

Cell Binding ◽

Bacterial Aggregation

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Design, Synthesis and Biological Evaluation of Novel Anthraniloyl-AMP Mimics as PQS Biosynthesis Inhibitors Against Pseudomonas aeruginosa Resistance

Molecules ◽

10.3390/molecules25133103 ◽

2020 ◽

Vol 25 (13) ◽

pp. 3103

Author(s):

Shekh Sabir ◽

Sujatha Subramoni ◽

Theerthankar Das ◽

David StC. Black ◽

Scott A. Rice ◽

...

Keyword(s):

Pseudomonas Aeruginosa ◽

Biofilm Formation ◽

Adenosine Monophosphate ◽

Biological Evaluation ◽

Bacterial Biofilms ◽

Design Synthesis ◽

Signaling Systems ◽

Design And Synthesis ◽

Bacterial Aggregation ◽

Synthesis And Biological Evaluation

The Pseudomonas quinolone system (PQS) is one of the three major interconnected quorum sensing signaling systems in Pseudomonas aeruginosa. The virulence factors PQS and HHQ activate the transcription regulator PqsR (MvfR), which controls several activities in bacteria, including biofilm formation and upregulation of PQS biosynthesis. The enzyme anthraniloyl-CoA synthetase (PqsA) catalyzes the first and critical step in the biosynthesis of quinolones; therefore, it is an attractive target for the development of anti-virulence therapeutics against Pseudomonas resistance. Herein, we report the design and synthesis of novel triazole nucleoside-based anthraniloyl- adenosine monophosphate (AMP) mimics. These analogues had a major impact on the morphology of bacterial biofilms and caused significant reduction in bacterial aggregation and population density. However, the compounds showed only limited inhibition of PQS and did not exhibit any effect on pyocyanin production.

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