scholarly journals Occurrence of Larval Dicrocoelium dendriticum and Brachylaima sp. in Gastropod Intermediate Hosts from Fergana Valley, Uzbekistan

2020 ◽  
Vol 52 (3) ◽  
Author(s):  
Abdurakhim Kuchboev ◽  
Mehmonjon Egamberdiev ◽  
Rokhatoy Karimova ◽  
Oybek Amirov ◽  
Mitsuhiko Asakawa
Keyword(s):  
Intermediate Hosts ◽  
Dicrocoelium Dendriticum

scholarly journals Contributions to and review of dicrocoeliosis, with special reference to the intermediate hosts of Dicrocoelium dendriticum

Parasitology ◽  
2001 ◽  
Vol 123 (7) ◽  
pp. 91-114 ◽  
Author(s):  
M. Y. MANGA-GONZÁLEZ ◽  
C. GONZÁLEZ-LANZA ◽  
E. CABANAS ◽  
R. CAMPO
Keyword(s):  
Special Reference ◽  
Epidemiological Study ◽  
Isoelectric Focusing ◽  
Infection Prevalence ◽  
Intermediate Hosts ◽  
Nw Spain ◽  
Flat Area ◽  
Dicrocoelium Dendriticum

An epidemiological study on dicrocoeliosis caused by Dicrocoelium dendriticum was carried out on sheep, molluscs and ants in the mountains of León province (NW Spain) between 1987–1991. The results concerning the intermediate hosts and a review of some aspects of dicrocoeliosis are summarized. Mollusc collection for the helminthological study was random throughout the study area at fortnightly intervals. Twenty-nine Gastropoda species were identified. D. dendriticum infection was only detected in 2·98% of the 2084 Helicella itala examined and in 1·06% of 852 H. corderoi. The highest infection prevalence was detected in H. itala in September and in H. corderoi in February. Daughter sporocysts with well-developed cercariae predominated in spring and autumn. Infection prevalence increased with mollusc age and size. Ants were collected from anthills or plants to which they were attached. The behaviour of ants in tetania was followed. Twenty-one Formicidae species were identified, but only the following harboured D. dendriticum: Formica cunicularia (1158 examined specimens, 0·69% infection prevalence, 2–56 metacercariae per ant); F. sanguinea (234, 1·28%, 2–63); F. nigricans (1770, 4·97%, 1–186); F. rufibarbis (288, 6·59%, 2–107). In a flat area close to León town, 95·39% of the 2085 F. rufibarbis specimens collected in tetania contained metacercariae (1–240) in the abdomen. These were used for parasite characterization by isoelectric focusing and to infect lambs and hamsters. Only one brainworm per ant was found.

scholarly journals Detection of Dicrocoelium dendriticum larval stages in mollusc and ant intermediate hosts by PCR, using mitochondrial and ribosomal internal transcribed spacer (ITS-2) sequences

Parasitology ◽  
2011 ◽  
Vol 138 (14) ◽  
pp. 1916-1923 ◽  
Author(s):  
A. M. MARTÍNEZ-IBEAS ◽  
M. MARTÍNEZ-VALLADARES ◽  
C. GONZÁLEZ-LANZA ◽  
B. MIÑAMBRES ◽  
M. Y. MANGA-GONZÁLEZ
Keyword(s):  
Polymerase Chain Reaction ◽  
Early Detection ◽  
Post Infection ◽  
Internal Transcribed Spacer ◽  
Intermediate Hosts ◽  
Chain Reaction ◽  
Accurate Identification ◽  
Dicrocoelium Dendriticum ◽  
Larval Stages ◽  
Polymerase Chain

SUMMARYThe aim of this study was to develop, perfect and validate the PCR (polymerase chain reaction) technique using mitochondrial (mt) and ribosomal (ITS-2) DNA for the accurate identification of Dicrocoelium dendriticum in molluscs and ants, the first and second intermediate hosts, and their early detection. The first primers that were designed amplified a 169 pb mtDNA fragment of D. dendriticum permitted the detection of a single D. dendriticum metacercaria from the Formica rufibarbis and Formica pratensis abdomen, as well as the detection of the brainworm in the head of the ants collected in tetania. Although these primers did not amplify Dicrocoelium chinensis DNA and permitted detected D. dendriticum in the molluscs, they did not discriminate Brachylaimidae metacercariae found in the same mollusc. The PCR that was designed to amplify a 93 bp fragment of the ITS-2 is D. dendriticum specific as it did not amplify D. chinensis, Brachylaimidae and other trematodes. This technique is very sensitive since it permitted the detection of D. dendriticum in the molluscs from the first day post-infection, the brainworm in the head of the ants and only 1 D. dendriticum metacercaria from the abdomen of the ants. Both techniques are important, mainly the latter.

Ultrastructure of Cercarial Tails

1974 ◽  
Vol 32 ◽  
pp. 180-181
Author(s):  
Richard S. Demaree ◽  
Donald M. Wootton
Keyword(s):  
Schistosoma Japonicum ◽  
Intermediate Hosts ◽  
Suitable Host ◽  
Ultrastructural Studies ◽  
Tail Structure

Cercariae (juvenile trematodes with tails) emerge from mollusk intermediate hosts and swim toward definitive hosts or encystment objects. The locomotor power is furnished by the tail. Upon reaching a suitable host or encystment object, the tail is cast off and the cercariae penetrate and/or encyst. Ultrastructural studies of cercariae are sparse. There is even lessUltrastructural studies of cercariae are sparse. There is even less information about the tail structure; and body-to-tail morphology has been documented only for Acanthatrium oregonense and Schistosoma japonicum.

EFFECT OF SOME ALGAL SPECIES ON THE SNAIL INTERMEDIATE HOSTS OF SCHISTOSOMIASIS IN EGYPT II. GROWTH, INFECTION AND MORTALITY RATES

2005 ◽  
Vol 6 (1) ◽  
pp. 93-110
Author(s):  
Faiza El-Assal ◽  
Sanaa Shanab ◽  
Amin Abou-El-Hassan ◽  
Kadria Mahmoud
Keyword(s):  
Algal Species ◽  
Mortality Rates ◽  
Intermediate Hosts

Taeniasis in a German Shepherd Pup: A Case Report

2019 ◽  
Vol 15 (01) ◽  
pp. 83-84
Author(s):  
B J Thakre ◽  
Joice P Joseph ◽  
Binod Kumar ◽  
Nilima Brahmbhatt ◽  
Krishna Gamit
Keyword(s):  
Case Report ◽  
Life Cycle ◽  
Definitive Host ◽  
Gastrointestinal Diseases ◽  
Intermediate Hosts ◽  
German Shepherd ◽  
Taenia Hydatigena ◽  
Taenia Multiceps ◽  
Taenia Crassiceps ◽  
Dipylidium Caninum

Taenia spp. are long, segmented, parasitic tapeworms and are relatively uncommon in canine gastrointestinal diseases compared to other tapeworms like Dipylidium caninum. These parasites have an indirect life cycle, cycling between definitive and intermediate hosts. Dogs act as definitive hosts of different species of Taenia including Taenia multiceps, Taenia serialis, Taenia crassiceps, Taenia hydatigena, Taenia pisiformis, etc. Taenia multiceps is of greatest zoonotic relevance in human. In the definitive host, it causes only mild infection. Larvae are more likely to cause disease than adult tapeworms. Taeniasis in pets should be cautiously handled because of its zoonotic importance. This communication reports a case of 3 months old pup suffering from Taenia infection that was successfully managed with a combination of praziquantel and fenbendazole.

Biology of Crassicutis cichlasomae, a parasite of cichlid fishes in Mexico and Central America

1995 ◽  
Vol 69 (1) ◽  
pp. 69-75 ◽  
Author(s):  
T. Scholz ◽  
M.C.F. Pech-Ek ◽  
R. Rodriguez-Canul
Keyword(s):  
Central America ◽  
Developmental Stages ◽  
Infected Fish ◽  
Constant Darkness ◽  
Intermediate Hosts ◽  
Cichlid Fishes ◽  
Feeding Experiments ◽  
Light Darkness ◽  
Higher Temperature ◽  
Cichlasoma Urophthalmus

AbstractField study on the biology of Crassicutis cichlasomae Manter, 1936 (Digenea: Homalometridae) was carried out in a small swamp in a limestone factory near Mérida, Yucatán, Mexico. Aquatic snails, Littorina (Littoridinopsis) angulifera, harbouring C. cichlasomae rediae, cercariae and metacercariae, served both as the first and second intermediate hosts. Feeding experiments confirmed the conspecificity of metacercariae from naturally infected snails with adults from naturally infected fish. Gravid C. cichlasomae worms were obtained from experimentally infected fish 19 days post exposure at 22–24°C. Examination of fish from the swamp in Mitza and other localities in the Yucatan Peninsula showed that the cichlids Cichlasoma urophthalmus and C. meeki were definitive hosts of C. cichlasomae. There was no pronounced preference of C. cichlasomae adults for the site of their location in the intestine of the definitive host; a slightly higher proportion (41%) of worms was only found in the anterior third of the gut. The time of miracidium development varied from 18.5 to 27.5 days; different temperature (20.1–35.7°C) or light/darkness regimes influenced only slightly the rate of embryonic development, with shorter development times at higher temperature (34.8–35.7°C) and constant darkness and/or light. With the exception of the sporocyst, all developmental stages are described and figured.

scholarly journals Molecular detection of cattle Sarcocystis spp. in North-West Italy highlights their association with bovine eosinophilic myositis

2021 ◽  
Vol 14 (1) ◽  
Author(s):  
Selene Rubiola ◽  
Tiziana Civera ◽  
Felice Panebianco ◽  
Davide Vercellino ◽  
Francesco Chiesa
Keyword(s):  
18S Rdna ◽  
Inflammatory Myopathy ◽  
Molecular Techniques ◽  
Diaphragm Muscle ◽  
Economic Losses ◽  
Intermediate Hosts ◽  
Slaughter Cattle ◽  
Significant Difference ◽  
Pcr Targeting

Abstract Background Cattle are intermediate hosts of six Sarcocystis species, among which Sarcocystis hominis and Sarcocystis heydorni can infect humans through the consumption of raw or undercooked meat. In addition to the zoonotic potential, there is increasing interest in these protozoa because of the evidence supporting the role of Sarcocystis spp. in the occurrence of bovine eosinophilic myositis (BEM), a specific inflammatory myopathy which leads to carcass condemnation and considerable economic losses. Actually, all the prevalence studies carried out on cattle in Italy have been based on either morphological or 18S rDNA-based molecular techniques, most likely leading to misidentification of closely related species. Therefore, there is a strong need for new data on the prevalence of the different Sarcocystis spp. in cattle in Italy and their association with bovine eosinophilic myositis. Methods To reach our aim, individual striated muscle samples from BEM condemned carcasses (N = 54) and diaphragm muscle samples from randomly sampled carcasses (N = 59) were obtained from Northwest Italy slaughterhouses. Genomic DNA was extracted and analyzed by multiplex-PCR targeting 18S rDNA and cox1 genes. PCR products amplified using the genus-specific primer set in absence of the specific fragment for S. hirsuta, S. cruzi, S. hominis or S. bovifelis were sequenced to achieve species identification. Results Sarcocystis DNA was detected in 67.8% of the samples from slaughter cattle and in 90.7% of the samples from BEM condemned carcasses. S. cruzi was identified as the most prevalent species in slaughter cattle (61%), followed by S. bovifelis (10.2%), S. hominis (8.5%) and S. hirsuta (1.7%). Notably, among the different Sarcocystis spp. detected, the presence of S. bovifelis and S. hominis was significantly higher in samples isolated from BEM condemned carcasses (46.3% and 40.7% respectively), while there was no statistically significant difference between the presence of S. cruzi or S. hirsuta in BEM condemned carcasses (42.6% and 1.8%, respectively) and randomly sampled carcasses. Furthermore, DNA sequence analysis revealed the presence of a putative new species in two carcasses. Conclusions Our study contributes to updating the data on the prevalence of the different Sarcocystis spp. in cattle in Italy, highlighting the presence of three Sarcocystis spp., S. cruzi, S. hominis and S. bovifelis, in BEM lesions and allowing us to speculate on the possible role of S. hominis and S. bovifelis as the major sarcosporidian species involved in bovine eosinophilic myositis. Graphic Abstract

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