INFLUENCE OF NITROGEN CONTENT AND CYTOKININ SOURCE ON PRUNUS DOMESTICA L. PROLIFERATION AND ELONGATION IN VITRO

1998 ◽  
pp. 341-346 ◽  
Author(s):  
V. Vasar ◽  
A. Pae ◽  
H. Jänes
Keyword(s):  
Nitrogen Content ◽  
Prunus Domestica

scholarly journals Effects of Applying Lactic Acid Bacteria and Molasses on the Fermentation Quality, Protein Fractions and In Vitro Digestibility of Baled Alfalfa Silage

Agronomy ◽  
2021 ◽  
Vol 11 (1) ◽  
pp. 91
Author(s):  
Yixiao Xie ◽  
Jinze Bao ◽  
Wenqi Li ◽  
Zhiqiang Sun ◽  
Run Gao ◽  
...  
Keyword(s):  
Production Process ◽  
Nitrogen Content ◽  
Gas Production ◽  
Protein Fractions ◽  
Fresh Matter ◽  
Fermentation Quality ◽  
Alfalfa Silage ◽  
True Protein ◽  
Nitrogen Contents

Alfalfa sometimes cannot be harvested in time due to the rainy season. To improve the fermentation quality, protein quality and digestibility of alfalfa silage harvested late, Lactobacillus plantarum (LP) and molasses were applied in an actual production process in this study. Alfalfa harvested at the full bloom stage was ensiled with (1) distilled water (control), (2) 1 × 106 colony-forming units LP/g fresh matter, (3) 15 g molasses/kg fresh matter (M) or (4) LP + M (LPM) for 55 days. Alfalfa ensiled with LP and/or molasses showed significantly lower pH and ammonia nitrogen contents than the control silage (p < 0.05). All additive treatments decreased nonprotein nitrogen contents and preserved more true protein (p < 0.05). However, molasses increased the acid detergent insoluble nitrogen content in the protein fractions (p < 0.05). The LP significantly improved the maximal cumulative gas production and the maximum gas production rate (p < 0.05) in the in vitro trial. Finally, both LP and molasses improved the neutral detergent fiber digestibility of the alfalfa silage (p < 0.05). The LP and molasses improved fermentation quality and digestibility and preserved more true protein in baled alfalfa silage harvested late in an actual production process. The LP utilized the excessive molasses and partially ameliorated its negative effects of causing higher acid detergent insoluble nitrogen content.

Varietal differences in in vitro dry matter digestibility in Setaria, and the effects of site, age, and season

1972 ◽  
Vol 23 (6) ◽  
pp. 959 ◽  
Author(s):  
JB Hacker ◽  
DJ Minson
Keyword(s):  
Nitrogen Content ◽  
Dry Matter ◽  
Ash Content ◽  
High Potassium ◽  
Dry Matter Digestibility ◽  
Flowering Behaviour ◽  
High Concentrations ◽  
Ranking Order ◽  
Cutting Regimes

Six Setaria introductions were grown in small plots at three sites differing in soil type and location in south-eastern Queensland, and fertilizer and irrigation water were applied at similar rates. All plots of each introduction were genetically identical. Three different cutting regimes were imposed, such that at 12-week intervals during the 2 years of the experiment 4, 8, and 12-week regrowth was available for comparison of in vitro dry matter digestibility. There was an overall difference in digestibility between varieties of 6.6 percentage units, and the ranking order was usually the same for all sites, seasons, and ages of regrowth. Three of the introductions were consistently superior to cv. Nandi, the only commercial cultivar included in the present study. The two Setaria splendida introductions ranked second and sixth, although they were both higher in ash concentration than the other introductions, and this was associated with high potassium content. Differences between varieties in digestibility were not associated with flowering differences, despite large differences in flowering behaviour. The maximum mean difference between sites was 4.9 units and this was associated with differences in ash content. Difference in ash content, however, accounted for only 23% of the digestibility difference. The high ash content was associated with high concentrations of potassium. There was no association between nitrogen content and digestibility. The introduction lowest in digestibility had the highest nitrogen content. Digestibility was higher in winter than in summer at all sites, and the extent of this difference increased with age of regrowth. Higher digestibilities were obtained in younger regrowth.

Properties of roughage feedstuffs collected from oesophageal fistulas.

1961 ◽  
Vol 1 (3) ◽  
pp. 159 ◽  
Author(s):  
WR McManus
Keyword(s):  
Nitrogen Content ◽  
Ash Content ◽  
Orifice Diameter ◽  
In Vitro Treatment

Observations on the nature of two roughage diets collected from nine oesophageal fistulated, pen-fed sheep on twenty-three occasions are reported. The diets were lucerne chaff and a wheaten grain-chaff mixture. The fistulas were of three types. Six were latex plug stoppered fistulas with relaxed orifice diameter of 1.5 to 3.0 cm ; two were fitted with rigid screw-capped acrylic cannulas of diameter 2.5 cm ; and one was an early Torell type fistula with a 6.0 cm by 1.0 cm orifice. Over 35 per cent of the diet can be recovered from these fistulas, which is satisfactory. Mean recoveries (pooled with respect to diet) were 36 per cent for acrylic cannuluted fistulas, 46 per cent for latex plug stoppered fistulas, and 82 per cent for the Torell type fistula. For all fistulas recovery values varied widely. On the lucerne chaff diet the nitrogen content of the extruded material did not differ significantly from the feed eaten. However, the ash content was significantly increased (P<0.05). On the diet of wheaten chaff and wheaten grain there was a slight, but not significant tenden9 for the extruded material to contain a higher ratio of chaff to grain. In vitro treatment of succulent grass and dry oaten straw with saliva confirmed that the increase in ash content was of salivary origin. There was no evidence that saliva either leached nitrogen from or added it to these feedstuffs

Studies on the nutritive value of the diet selected by grazing sheep IV. Variation in the diet selected by sheep differing in age, breed, sex, strain and previous history

1969 ◽  
Vol 11 (3) ◽  
pp. 369-378 ◽  
Author(s):  
J. P. Langlands
Keyword(s):  
Nitrogen Content ◽  
Nutritive Value ◽  
Previous History ◽  
In Vitro Digestibility ◽  
Sheep Grazing ◽  
Faecal Nitrogen ◽  
Grazing Sheep ◽  
Grazing History

SUMMARYThe ability of two groups of six Merino wethers aged 6 and 66 months to digest a lucerne ration was examined in order to detect possible biases in the use of faecal nitrogen as a faecal index substance. Earlier work with this technique had indicated a difference in selectivity between young and older sheep. No difference in ability to digest the ration was observed and it was concluded that the earlier observation was not an artefact in the faecal nitrogen technique. Oesophageal fistulae were prepared in 120 sheep differing in breed, sex, age, strain and previous history. Determinations were made of the nitrogen content, and in one experiment of the in vitro digestibility of the diet selected by sheep grazing various pastures. Significant differences in the nitrogen content of the diet selected by sheep varying in breed, age and previous grazing history were recorded on some pastures on some occasions but not on others. No significant differences were observed between sheep varying in strain or sex. No reason could be suggested for the occasional differences between groups in the nitrogen content or digestibility of the diet selected, but it appeared unlikely that it would be a major source of variation in the productivity of the different groups at pasture.

Characterization of Prunus domestica L. in vitro regeneration via hypocotyls

2007 ◽  
Vol 112 (4) ◽  
pp. 462-466 ◽  
Author(s):  
Lining Tian ◽  
Susan Sibbald ◽  
Jayasankar Subramanian ◽  
Antonet Svircev
Keyword(s):  
In Vitro Regeneration ◽  
Prunus Domestica

scholarly journals Regeneration capacity of Cerasus fruticosa and Prunus domestica into the in vitro culture

2021 ◽  
Vol 209 (06) ◽  
pp. 43-52
Author(s):  
Marina Markova ◽  
Elena Somova
Keyword(s):  
In Vitro Culture ◽  
Nutrient Medium ◽  
Optimal Time ◽  
In Vitro Cultivation ◽  
Initiation Time ◽  
Multiplication Factor ◽  
Optimal Conditions ◽  
Prunus Domestica ◽  
Nutrient Media

Abstract. The aim of these studies was to introduce into the in vitro culture the steppe cherry (Cerasus fruticosa) variety Shchedraya and the domestic plum (Prunus domestica) variety Sineokaya for subsequent micropropagation. Methods. Optimal conditions for obtaining viable explants, such as sterilizing agent and initiation time, have been investigated. The suitability of various nutrient media for in vitro cultivation of these cultures has also been tested. As a result of the experiments, it was revealed that the most effective sterilizing agents were 38 % perhydrol (control) and 6% chlorhexidine: the yield of viable cherry explants was 63.8 % and 61.5 %, plums – 69.8 % and 66.6 %, respectively. The optimal time for the initiation of cherry explants in vitro was January, where the yield of viable explants averaged 53.9 %, in June – 49.1 %, and for plums the initiation time did not matter – the yield of explants was 55.8 % in winter and 53.1 % in summer. In vitro cultivation of cherries and plums on the Quoirin – Lepoivre nutrient medium provided a significantly high multiplication factor, which averaged 4.1 for cherries (2.7 in control) and 6.0 for plums (3.9 in control). On the same medium, the maximum multiplication factor was obtained, which was 6.2 for cherries and 8.2 for plums. Thus, the scientific novelty of these studies is that the optimal conditions (sterilizing agent, time, nutrient medium) have been selected for the regeneration of cherry and plum explants in vitro with their subsequent micropropagation.

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