A 12-YEAR REVIEW OF ELISA MONITORING OF MAJOR POTATO VIRUSES IN DORMANT SEED-TUBERS IN BRAZIL

2003 ◽  
pp. 153-159 ◽  
Author(s):  
J.A. Caram de Souza-Dias ◽  
J.A. Betti
2003 ◽  
Vol 39 (2) ◽  
pp. 209-217 ◽  
Author(s):  
H. SHIWACHI ◽  
T. AYANKANMI ◽  
R. ASIEDU ◽  
M. ONJO

A reliable means to induce sprouting in dormant seed tubers of yams (Dioscorea species) is required to enhance flexibility in planting date and rate of propagation of the crop. Experiments were conducted to assess the potential of two gibberellin inhibitors, Uniconazole-P and Prohexadione-calcium, to induce sprouting in tubers from three varieties of D. rotundata and four of D. alata. Uniconazole-P and Prohexadione-calcium shortened the period of dormancy in tubers of some varieties. In others, they either had no effect or extended dormancy. The varietal responses were also influenced by whether the tubers were treated at harvest (before shoot senescence) or four weeks after harvest by which time shoots had senesced fully. Tubers stored in darkness at a constant temperature of 30 °C sprouted earlier but lost weight faster than did those stored under natural daylight and ambient temperature. The apparent slow action of Uniconazole-P and Prohexadione-calcium, and their variable effectiveness in relation to variety, would limit their usefulness in seed or breeding programmes, or in determining treatment timing and the best storage environment for the tubers after treatment.


2018 ◽  
Vol 10 (3) ◽  
pp. 71
Author(s):  
Gidraf Onduru Okeyo ◽  
Kalpana Sharma ◽  
Elly Atieno ◽  
Rama Devi Narla ◽  
Douglas Watuku Miano ◽  
...  

Potato productivity in Kenya has remained below the potential due to the use of infected seed tubers especially by seed borne viruses. A study was carried out to asses the effectiveness of Positive Selection (PS) in managing seed borne potato viruses. Sprouted seed potato tubers of nine genotypes; three commercial varieties and six advanced International Potato Center (CIP) clones were screened in the field for two seasons, Field Generation Three (FG3) and Field Generation Four (FG4), respectively. In each season, experimental plots were divided into two sub-plots in which one sub-plot was planted with seed tubers sourced through Random Seed Selection (RSS) and the other with seed tuber sourced through Positive Selection (PS). Positive selection plots had low visual virus incidences, high number of tubers per hill and yield (t/ha) in both FG3 and FG4 growing seasons irrespective of the genotypes. ELISA test also revealed that Potato Virus S (PVS) (78%) was the most predominant virus followed by Potato Virus Y (PVY) (20%), Potato Leaf Roll Virus (PLRV) (16%) and Potato Virus M (PVM) (9%) in RSS plots either with single or multiple infections. Positive Selection plots recorded low virus incidences of PVS (47%), and PVY, PLRV and PVM were absent in the tubers tested with ELISA. This study concludes that Positive Selection is a good management strategy to manage the seed borne potato viruses, and if adopted, small holder farmers can reduce the yield losses arising from use of virus infected seed tubers.


1984 ◽  
Vol 103 (3) ◽  
pp. 487-495 ◽  
Author(s):  
H. S. Sekhon ◽  
M. Singh

SummaryField experiments to study the effect of pre-sprouting, cutting seed and seed soaking in plant regulators on the number and size of seed tubers and yield of potatoes were conducted during the autumn crop seasons of 1978–9 to 1980–1. At the same seed rate, cut seed produced significantly more sprouts and stems, seed-size tubers and seed yield than the whole seed without affecting the aggregate yield. Similar effects were recorded with soaking whole non-dormant tubers in 10 mg GA 3/1 solution for 15 min before planting. Soaking whole non-dormant seed in 0·5% thiourea solution for 1 h increased the number of sprouts and seed-size tubers, giving increases in seed yield of 2·26, 2·73 and 2·65 t/ha over soaking seed in water in the three consecutive years. The corresponding increases in aggregate yield were 1·79, 1·48 and 1·60 t/ha. Soaking cut seed in solutions of 10 mg GA 3/1 and 0·5% thiourea singly or in combination had an adverse effect on the yield of potatoes.


2017 ◽  
Vol 75 (1) ◽  
pp. 63-70
Author(s):  
Krystyna Michalak ◽  
Mirosława Chrzanowska

Abstract The paper describes testing potato cultivars for resistance to potato viruses in field and greenhouse conditions according to the scheme of the statutory trials. Seed tubers of tested cultivar are planted in the field in two replications with 30 tubers each. On both sides of each cultivar, 15 PVY-infected tubers, i.e. the source for PVY inoculation, are planted. The evaluation includes standard cultivars of known resistance levels to PVY. The progeny tubers are collected in autumn and growing-on testing is carried out in greenhouse conditions to estimate the number of plants infected with PVY. The other method is to evaluate the resistance of potato cultivars in greenhouse conditions. At present, the resistance of cultivars is evaluated only with respect to PVY, the most important potato virus. Two strains are used in the evaluation, i.e. PVYN-Wiand PVYNTN. For each testing cultivar, 10 plants are mechanically inoculated with each PVY strain. The analysis of the results makes it possible to classify the cultivars into four resistance groups on a 1-9 scale: highly resistant (with the resistance score of 8), resistant (with a value of 7), mid-resistant (with a value 5-6), and susceptible (with a value of 3-4). The ”9” rating is specified after the registration of the cultivars based on molecular tests, that validate the presence of a marker linked to extreme resistance (ER) gene Rysto.


1993 ◽  
Vol 89 (2) ◽  
pp. 262-270 ◽  
Author(s):  
G. N. Mohan Kumar ◽  
N. Richard Knowles

Author(s):  
R.A. Bagrov ◽  
◽  
V.I. Leunov

The mechanisms of transmission of potato viruses from plants to aphid vectors and from aphids to uninfected plants are described, including the example of the green peach aphid (Myzus persicae, GPA). Factors affecting the spreading of tuber necrosis and its manifestation on plants infected with potato leafroll virus (PLRV) are discussed. Recommendations for PLRV and GPA control in the field are given.


2021 ◽  
Vol 113 ◽  
pp. 101604
Author(s):  
Pablo Gutiérrez ◽  
Ary Rivillas ◽  
Daniel Tejada ◽  
Susana Giraldo ◽  
Andrea Restrepo ◽  
...  

1987 ◽  
Vol 67 (2) ◽  
pp. 599-603 ◽  
Author(s):  
A. W. McKEOWN

Shoots of the early-maturing potato (Solatium tuberosum L.) cultivar Jemseg often do not emerge uniformly when cut seed pieces are planted in cold soil. Under controlled temperatures of 10, 15 and 20 °C, shoots from whole tubers and basal portions of Jemseg tubers emerged later than those from apical portions of seed tubers. The delayed emergence of the basal portion was most pronounced at low temperatures and ranged from 5 to 17 d. Emergence from basal vs. apical portions of Conestoga was delayed by 10 d at 10 °C and by 4 d at 15 °C, only when seed tubers with a single dominant apical sprout were used. There was no delay at 20 °C. The variable time to emergence of shoots in the field can be explained by delayed emergence from basal buds on cut seed pieces.Key words: Potato, cultivars, emergence, bud position, temperature, seed tuber


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