PRELIMINARY SCREENING OF INDIGENOUS BACTERIAL ISOLATES FROM SOIL AND FISH POND SEDIMENTS FOR THEIR BENEFICIAL EFFECT ON VEGETABLES GROWTH AND HEALTH

2012 ◽  
pp. 355-362
Author(s):  
I. Tringovska ◽  
D. Kostova ◽  
S. Masheva ◽  
V. Yankova ◽  
G. Pasev
2020 ◽  
Vol 11 (10) ◽  
pp. 5151-5163
Author(s):  
D. Dróżdż ◽  
K. Malińska ◽  
M. Kacprzak ◽  
M. Mrowiec ◽  
A. Szczypiór ◽  
...  

Abstract Increased fish pond production is associated with the generation of various waste including fish pond sediments. Fish pond sediments could be a valuable source of nutrients for growing plants, however they require further processing in order to be applied to soil. Composting is considered one of the methods for processing fish pond sediments. The aim of this work was to investigate the potential of organic fish pond sediments to be managed through laboratory composting with selected waste materials and evaluate the fertilizing potential of the obtained compost. The scope included: (1) analysis of organic fish pond sediments, (2) laboratory composting of organic fish pond sediments with wheat straw and green grass, (3) analysis of the obtained composts, (4) preparation of growing media with selected additives, (5) analysis of the properties of the prepared growing media and (6) analysis of the effect of the investigated growing media on the growth of Phaseolus vulgaris L. in pot experiments. The addition of cardboard waste and woodchips derived biochar to the obtained compost improved significantly the growth of the roots of Cardamine L. Also, the content of carbon and nitrogen in the compost mixtures increased. The addition of M1 (compost from organic fish pond sediments and 1% of biochar) to the soil had a significant impact on the growth of white beans. Graphic Abstract


Author(s):  
Kawo Abdullahi Hassan ◽  
Yahaya Sani ◽  
Olawore Yemisi Ajoke

Spent motor oil represents one of the most prominent and tenacious contaminants of soil within mechanic workshops across Nigeria. Adaptation and natural evolution proffer a rich array of a microorganism capable of producing bio-surfactants which are of high value to industry, particularly in hydrocarbon degradation. This study was undertaken to isolate and screen for bio-surfactant producing bacterial strains isolated from engine oil contaminated mechanic sites. Data obtained revealed that using 0.3% Fluconazole supplemented nutrient agar media, bacterial isolates were obtained from soil samples within Apo mechanic village, Abuja, Nigeria. Hemolytic assay method and foam capacity test were used for screening. The positive strains were grown in liquid medium and the emulsification index (EI24) was determined. A total of 3 bacterial isolates referred to as A2, B1 and C8 were positive for the hemolytic and foam capacity tests, with an emulsification index (EI24) of 40.0, 11.7 and 36.7 respectively. Growth measurements were determined by measuring optical density of the cells in broth using spectrophotometer at 605nm over a 96 hour incubatory period in nutrient broth at 4ºC, 37ºC, 42ºC. All isolates displayed mesophilic characteristics withprogressive growth. At 37ºC, isolate A2 had the highest growth rate via optical density readout 1.95, 2.11, 2.32, 2.55, measured at 605nm. Isolate A2 also the best performing isolate at 42ºC with optical density readings of 1.32, 1.00, 0.91 and 1.68, thereby suggestive of thermotolerant ability. All isolates demonstrated good growth in broth medium with pH ranging from 8.97-9.16 at 37ºC. All isolates were positive for catalase and citrate, negative for methyl red, Voges-Proskauer and indole tests. Isolate A2 was the only gram-positive, oxidase negative and non-motile bacteria. The bio-surfactants produced by the three different bacterial isolates would possess chemically distinct signatures that can be harnessed for multiple applications ranging from bioremediation to degradable detergent uses.


2016 ◽  
Vol 68 (4) ◽  
pp. 845-852 ◽  
Author(s):  
Olja Stanojevic ◽  
Svetlana Milijasevic-Marcic ◽  
Ivana Potocnik ◽  
Milos Stepanovic ◽  
Ivica Dimkic ◽  
...  

The isolation of bacteria was carried out from samples of straw and chicken manure, compost at various stages of the composting process and casing soil used for growing button mushrooms. A preliminary screening of 108 bacterial isolates for antagonistic activity against Trichoderma aggressivum f. europaeum showed that 23 tested isolates inhibited mycelial growth of the pathogenic fungus. Further screening with four indicator isolates of fungi revealed that all 23 bacterial isolates inhibited the growth of T. aggressivum f. europaeum, T. harzianum and T. koningii, while only 13 isolates inhibited the growth of T. atroviride. T. aggressivum f. europaeum proved to be the most sensitive, with many bacterial isolates generating a high percentage of growth inhibition. Only two bacterial isolates (B-129 and B-268) were successful in inhibiting the growth of all 4 tested pathogens. All 23 bacterial isolates were characterized as Gram-positive and catalase-positive and were subjected to molecular identification based on the partial sequence, the hypervariant region of the 16S rDNA. It was shown that the obtained bacterial strains belong to Bacillus subtilis, B. amyloliquefaciens, B. licheniformis and B. pumilus species.


Author(s):  
M. O. Ojo

More often than not, the emphasis is laid on the essence of employing organic manures for raising plant seedlings and even in improving the nutrient status of their growth media for higher productivity. Afrormosia elata has numerous medicinal uses but not very much available. Thus, the study on the effects of fish pond sediments (FPS) and decomposed cow dung (DCD) on the early growth of A. elata seedlings was carried out at the nursery ‘A’ of the Federal College of Forestry, Ibadan, Nigeria. A. elata seeds were sown in a finely perforated sieve (filled with washed river sand) and seedlings were pricked – out 2 weeks after seedling emergence into polythene pots with varying levels of FPS and DCD. The experimental design was Completely Randomized Design (CRD) consisting of nine treatments and eight replicates. Treatments include; T1(2 kg of FPS + 2 kg of topsoil); T2 (2 kg of DCD + 2 kg of topsoil); T3 (1.5 kg of FPS + 2 kg of topsoil); T4 (1.5 kg of DCD + 2 kg of topsoil); T5 (1 kg of FPS + 2 kg of topsoil); T6 (1 kg of DCD + 2 kg of topsoil); T7 (500 g of FPS + 2 kg of topsoil); T8 (500 g of DCD + 2 kg topsoil); and 2 kg of topsoil without any treatment served as control). Morphological parameters such as seedling height, collar diameter and leaf count as well as leaf biomass were assessed and the data collected were subjected to Analysis of Variance (ANOVA). The result showed that T3 (1.5 kg FPS + 2 Kg TS) had the best performance in height, leaf area and leaf biomass with mean values of 11.02 cm, 21.65 cm2 and 1.16 g respectively. Though, there were no significant differences amongst the growth parameters assessed for this study. But T3 (1.5 kg FPS + 2 Kg TS) could be employed in raising the seedlings of this plant for faster growth rate.


Agronomy ◽  
2020 ◽  
Vol 10 (7) ◽  
pp. 1025
Author(s):  
Chau Thi Da ◽  
Phan Anh Tu ◽  
John Livsey ◽  
Van Tai Tang ◽  
Håkan Berg ◽  
...  

The increasing intensification of aquaculture systems requires the development of strategies to reduce their environmental impacts such as pollution caused by the discharge of nutrient rich sediments into local water bodies. Recycling of fish pond sediments (FPS) as fertilizer has been proposed as a possible solution that may also reduce the reliance on synthetic fertilizers. With a case study in the Mekong Delta, Vietnam, we determined suitable mixtures of striped catfish (Pangasianodon hypophthalmus) pond sediment (PPS) and locally sourced organic amendments of rice straw (RS), or common water hyacinth (WH) to fertilize cucumber plants (Cucumis sativus L.) in an integrated cucumber–giant gourami fish (Osphronemus goramy) farming system. Highest nutrient concentrations were found when mixing 30% PPS with 70% RS or WH. When used in combination with chemical fertilizer, it was found that a 25% to 75% reduction in chemical fertilizer application could be achieved, while also increasing cucumber yields, with the highest yields found when RS was used in organic amendments. In combination with the additional income from fish production, integrated farming systems such as that demonstrated in this study, may increase both farm income and production diversity.


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