Comparison of waterlogging tolerance between Actinidia macrosperma and Actinidia deliciosa

Low oxygen level is a phenomenon often occurring during the cucumber cultivation period. Genes involved in adaptations to stress can be regulated by non-coding RNA. The aim was the identification of long non-coding RNAs (lncRNAs) involved in the response to long-term waterlogging stress in two cucumber haploid lines, i.e., DH2 (waterlogging tolerant—WL-T) and DH4 (waterlogging sensitive—WL-S). Plants, at the juvenile stage, were waterlogged for 7 days (non-primed, 1xH), and after a 14-day recovery period, plants were stressed again for another 7 days (primed, 2xH). Roots were collected for high-throughput RNA sequencing. Implementation of the bioinformatic pipeline made it possible to determine specific lncRNAs for non-primed and primed plants of both accessions, highlighting differential responses to hypoxia stress. In total, 3738 lncRNA molecules were identified. The highest number (1476) of unique lncRNAs was determined for non-primed WL-S plants. Seventy-one lncRNAs were depicted as potentially being involved in acquiring tolerance to hypoxia in cucumber. Understanding the mechanism of gene regulation under long-term waterlogging by lncRNAs and their interactions with miRNAs provides sufficient information in terms of adaptation to the oxygen deprivation in cucumber. To the best of our knowledge, this is the first report concerning the role of lncRNAs in the regulation of long-term waterlogging tolerance by priming application in cucumber.

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Identification and Functional Analysis of ThADH1 and ThADH4 Genes Involved in Tolerance to Waterlogging Stress in Taxodium hybrid ‘Zhongshanshan 406’

Genes ◽

10.3390/genes12020225 ◽

2021 ◽

Vol 12 (2) ◽

pp. 225

Author(s):

Lei Xuan ◽

Jianfeng Hua ◽

Fan Zhang ◽

Zhiquan Wang ◽

Xiaoxiao Pei ◽

...

Keyword(s):

Ethanol Metabolism ◽

Flooding Tolerance ◽

Transcriptome Data ◽

Low Oxygen ◽

Anaerobic Conditions ◽

Waterlogging Tolerance ◽

Waterlogging Stress ◽

Adh Genes ◽

Oxygen Conditions ◽

Stems And Leaves

The Taxodium hybrid ‘Zhongshanshan 406’ (T. hybrid ‘Zhongshanshan 406’) [Taxodium mucronatum Tenore × Taxodium distichum (L.). Rich] has an outstanding advantage in flooding tolerance and thus has been widely used in wetland afforestation in China. Alcohol dehydrogenase genes (ADHs) played key roles in ethanol metabolism to maintain energy supply for plants in low-oxygen conditions. Two ADH genes were isolated and characterized—ThADH1 and ThADH4 (GenBank ID: AWL83216 and AWL83217—basing on the transcriptome data of T. hybrid ‘Zhongshanshan 406’ grown under waterlogging stress. Then the functions of these two genes were investigated through transient expression and overexpression. The results showed that the ThADH1 and ThADH4 proteins both fall under ADH III subfamily. ThADH1 was localized in the cytoplasm and nucleus, whereas ThADH4 was only localized in the cytoplasm. The expression of the two genes was stimulated by waterlogging and the expression level in roots was significantly higher than those in stems and leaves. The respective overexpression of ThADH1 and ThADH4 in Populus caused the opposite phenotype, while waterlogging tolerance of the two transgenic Populus significantly improved. Collectively, these results indicated that genes ThADH1 and ThADH4 were involved in the tolerance and adaptation to anaerobic conditions in T. hybrid ‘Zhongshanshan 406’.

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EFFECT OF A CYTOKININ-LIKE COMPOUND (CPPU) ON KIWIFRUIT (ACTINIDIA DELICIOSA) RIPENING AND STORAGE LIFE

Acta Horticulturae ◽

10.17660/actahortic.1995.379.52 ◽

1995 ◽

pp. 421-428 ◽

Cited By ~ 1

Author(s):

G. Costa ◽

R. Biasi ◽

S. Brigati ◽

M. Morigi ◽

E. Antognozzi

Keyword(s):

Storage Life ◽

Actinidia Deliciosa ◽

And Storage

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Functional Characterization of Waterlogging and Heat Stresses Tolerance Gene Pyruvate decarboxylase 2 from Actinidia deliciosa

International Journal of Molecular Sciences ◽

10.3390/ijms18112377 ◽

2017 ◽

Vol 18 (11) ◽

pp. 2377 ◽

Cited By ~ 4

Author(s):

Hui-Ting Luo ◽

Ji-Yu Zhang ◽

Gang Wang ◽

Zhan-Hui Jia ◽

Sheng-Nan Huang ◽

...

Keyword(s):

Functional Characterization ◽

Pyruvate Decarboxylase ◽

Actinidia Deliciosa ◽

Tolerance Gene

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A somaclonal myrobalan rootstock increases waterlogging tolerance to peach cultivar in controlled conditions

Scientia Horticulturae ◽

10.1016/j.scienta.2013.03.014 ◽

2013 ◽

Vol 156 ◽

pp. 1-8 ◽

Cited By ~ 9

Author(s):

Calogero Iacona ◽

Marco Cirilli ◽

Alessandra Zega ◽

Eleonora Frioni ◽

Cristian Silvestri ◽

...

Keyword(s):

Waterlogging Tolerance ◽

Peach Cultivar ◽

Controlled Conditions

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Experimental comparison of two methods to study barley responses to partial submergence

Plant Methods ◽

10.1186/s13007-021-00742-5 ◽

2021 ◽

Vol 17 (1) ◽

Author(s):

Alexandra Miricescu ◽

Tomás Byrne ◽

Catherine M. Doorly ◽

Carl K. Y. Ng ◽

Susanne Barth ◽

...

Keyword(s):

Global Climate ◽

Experimental Comparison ◽

Soil Conditions ◽

Early Developmental Stage ◽

Starch Solution ◽

Waterlogging Tolerance ◽

Climate Conditions ◽

Flooding Events ◽

Staple Crop ◽

Early Developmental

Abstract Background Crop yield is dependent on climate conditions, which are becoming both more variable and extreme in some areas of the world as a consequence of global climate change. Increased precipitation and flooding events are the cause of important yield losses due to waterlogging or (partial) submergence of crops in the field. Our ability to screen efficiently and quickly for varieties that have increased tolerance to waterlogging or (partial) submergence is important. Barley, a staple crop worldwide, is particularly sensitive to waterlogging. Screening for waterlogging tolerant barley varieties has been ongoing for many years, but methods used to screen vary greatly, from the type of soil used to the time at which the treatment is applied. This variation makes it difficult to cross-compare results. Results Here, we have devised a scoring system to assess barley tolerance to waterlogging and compare two different methods when partial submergence is applied with either water or a starch solution at an early developmental stage, which is particularly sensitive to waterlogging or partial submergence. The use of a starch solution has been previously shown to result in more reducing soil conditions and has been used to screen for waterlogging tolerance. Conclusions Our results show that the two methods provide similar results to qualitatively rank varieties as tolerant or sensitive, while also affecting plants differently, in that application of a starch solution results in stronger and earlier symptoms than applying partial submergence with water.

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Long-Term Waterlogging as Factor Contributing to Hypoxia Stress Tolerance Enhancement in Cucumber: Comparative Transcriptome Analysis of Waterlogging Sensitive and Tolerant Accessions

Genes ◽

10.3390/genes12020189 ◽

2021 ◽

Vol 12 (2) ◽

pp. 189

Author(s):

Kinga Kęska ◽

Michał Wojciech Szcześniak ◽

Izabela Makałowska ◽

Małgorzata Czernicka

Keyword(s):

Stress Tolerance ◽

De Novo ◽

Average Length ◽

Marker Genes ◽

Long Term Memory ◽

Low Oxygen ◽

Waterlogging Tolerance ◽

Pre Treatment ◽

Waterlogging Treatment

Waterlogging (WL), excess water in the soil, is a phenomenon often occurring during plant cultivation causing low oxygen levels (hypoxia) in the soil. The aim of this study was to identify candidate genes involved in long-term waterlogging tolerance in cucumber using RNA sequencing. Here, we also determined how waterlogging pre-treatment (priming) influenced long-term memory in WL tolerant (WL-T) and WL sensitive (WL-S) i.e., DH2 and DH4 accessions, respectively. This work uncovered various differentially expressed genes (DEGs) activated in the long-term recovery in both accessions. De novo assembly generated 36,712 transcripts with an average length of 2236 bp. The results revealed that long-term waterlogging had divergent impacts on gene expression in WL-T DH2 and WL-S DH4 cucumber accessions: after 7 days of waterlogging, more DEGs in comparison to control conditions were identified in WL-S DH4 (8927) than in WL-T DH2 (5957). Additionally, 11,619 and 5007 DEGs were identified after a second waterlogging treatment in the WL-S and WL-T accessions, respectively. We identified genes associated with WL in cucumber that were especially related to enhanced glycolysis, adventitious roots development, and amino acid metabolism. qRT-PCR assay for hypoxia marker genes i.e., alcohol dehydrogenase (adh), 1-aminocyclopropane-1-carboxylate oxidase (aco) and long chain acyl-CoA synthetase 6 (lacs6) confirmed differences in response to waterlogging stress between sensitive and tolerant cucumbers and effectiveness of priming to enhance stress tolerance.

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