scholarly journals Immune status in bronchopulmonary dysplasia

2016 ◽  
Vol 97 (3) ◽  
pp. 346-350 ◽  
Author(s):  
I A Mustafayev ◽  
L I Allakhverdieva ◽  
A V Bogdanova
Keyword(s):  
Immune Response ◽  
Bronchopulmonary Dysplasia ◽  
Reference Values ◽  
Immune Complexes ◽  
Interferon Γ ◽  
Circulating Immune Complexes ◽  
Interleukin 4 ◽  
Reference Ranges ◽  
Suppressor Activity ◽  
Remission Phase

Aim. To study cell-mediated and humoral immunity in bronchopulmonary dysplasia in children.Methods. The inpatient and outpatient medical records of 103 children from the 1st day of life up to 3 years of age: 58 boys and 45 girls, were analyzed. T-lymphocytes helper and suppressor activity markers, the immunoregulatory index, B-lymphocytes markers, concentration of the three main immunoglobulins classes, pro- and anti-inflammatory interleukins, circulating immune complexes were determined.Results.. The helper activity in exacerbation phase was at the lower limit of normal (42.1±0.9) and significantly increased in remission phase (52.6±0.8), without exceeding the reference values. CD8 lymphocytes percentage in the blood remained within the reference ranges both in exacerbation of inflammation and in remission phase. Immunoregulatory index was above normal both in the exacerbation and in the remission phases, reducing in remission phase. Pro-inflammatory interleukin-4 concentration in the exacerbation period exceeded reference values by 1.5 times and amounted to 21.0±0.6 pg/ml. In the remission phase its normalization (11.1±0.4 pg/ml) appeared. Interleukin-8 and interferon-γ levels in the exacerbation stage were significantly higher than normal and amounted to 70.3±1.2 and 15.1±0.4 pg/ml, respectively. The tumor necrosis factor concentration was at the upper limit of normal (19.54±0.29 pg/ml). The humoral immune response was characterized by a slight decrease in the immunoglobulin A level, some increase in immunoglobulin G and a significant increase in the circulating immune complexes concentration (109.5±6.6 units) in the exacerbation phase.Conclusion. Pronounced immunosuppression is uncharacteristic for bronchopulmonary dysplasia; identified changes are an adequate immune response to viral and bacterial infection in the acute phase of disease.

PARTICULARITIES OF THE IMMUNE RESPONSE IN PERSONS PROFESSIONNALLY EXPOSED TO BERYLLIUM

2016 ◽  
pp. 26-30
Author(s):  
K. I. Stosman ◽  
L. V. Lukovnikova
Keyword(s):  
Immune Response ◽  
Immune Complexes ◽  
Immunoglobulin E ◽  
Circulating Immune Complexes ◽  
Professional Contact ◽  
Beryllium Compounds

An examination was performed of 50 employees at an enterprise where they were in professional contact with beryllium. In most workers, it was detected an increase of interleukine-8, interferon- , growing level of immunoglobulin E and circulating immune complexes. It was shown that the contact with beryllium compounds leads to the interferon- level growth only in women. In men, alterations are identified in the direction of increased concentrations of common immunoglobulin E and circulating immune complexes.

scholarly journals Reciprocal Protective Immunity againstBordetella pertussis and Bordetella parapertussis in a Murine Model of Respiratory Infection

2001 ◽  
Vol 69 (11) ◽  
pp. 6981-6986 ◽  
Author(s):  
Mineo Watanabe ◽  
Masaaki Nagai
Keyword(s):  
Immune Response ◽  
Respiratory Infection ◽  
Murine Model ◽  
Protective Immunity ◽  
Interferon Γ ◽  
Interleukin 4 ◽  
Spleen Cells ◽  
Bordetella Parapertussis ◽  
Ifn Γ

ABSTRACT The protective immunity induced by infection with Bordetella pertussis and with Bordetella parapertussis was examined in a murine model of respiratory infection. Convalescent mice that had been infected by aerosol with B. pertussis or with B. parapertussis exhibited a protective immune response against B. pertussis and also against B. parapertussis. Anti-filamentous hemagglutinin (anti-FHA) serum immunoglobulin G (IgG) and anti-FHA lung IgA antibodies were detected in both mice infected with B. pertussis and those infected with B. parapertussis. Antibodies against pertussis toxin (anti-PT) and against killed B. pertussis cells were detected in mice infected with B. pertussis. Pertactin-specific antibodies and antibodies against killed B. parapertussis cells were detected in mice infected with B. parapertussis. Spleen cells from mice infected with B. pertussis secreted interferon-γ (IFN-γ) in response to stimulation by FHA or PT. Spleen cells from mice infected with B. parapertussis also secreted IFN-γ in response to FHA. Interleukin-4 was not produced in response to any of the antigens tested. The profiles of cytokine secretion in vitro revealed induction of a Th1-biased immune response during convalescence from infection by B. pertussis and byB. parapertussis. It is possible that Th1 and Th2 responses against FHA might be related to the reciprocal protection achieved in our murine model.

scholarly journals Immune response in new coronavirus infection COVID-19 in children and adults

2021 ◽  
Vol 20 (4) ◽  
pp. 12-17
Author(s):  
O. P. Kovtun ◽  
O. M. Olenkova ◽  
J. B. Beikin
Keyword(s):  
Immune Response ◽  
T Lymphocytes ◽  
Immune Complexes ◽  
Process Development ◽  
Acute Infection ◽  
Age Groups ◽  
Circulating Immune Complexes ◽  
Similar Data ◽  
Activated T Lymphocytes ◽  
Coronavirus Infection

Introduction. It is known that COVID-19 occurs more often in adult patients, especially if they have concomitant somatic diseases, children are at less risk of developing it. The aim of this work is to evaluate and compare immune response parameters in new coronavirus infection COVID-19 in children and adults. Materials and methods. The results of the examination of 56 adults 19-55 years old and 57 children 14-18 years old were included in the work. The examination results of COVID-19 patients were compared with similar data in practically healthy individuals of the corresponding age groups. All patients underwent laboratory tests to determine the following parameters: presence of SARS-CoV-2 RNA, general blood test parameters, major lymphocyte subpopulations, level of total immunoglobulins (IgM, IgG, IgA), number of CIC, absorbance and bactericidal activity of leukocytes.Results. The proportion of positive findings for SARS-CoV-2 RNA in different age groups ranged from 13.6% to 25.8%. General and specific patterns of immune response in patients of different age groups were established. Common features were an increase in the number of circulating immune complexes and activated T-lymphocytes. Differences were noted in the level of serum immunoglobulins IgM and IgG, neutrophil uptake activity, the number of monocytes, as well as in the level of different subpopulations of lymphocytes. Discussion. In adults, changes in the adaptive immune response, including the cellular level, are predominantly expressed. In children, there are signs of inefficiency of innate mechanisms of immune responses. Conclusion. The dynamics of the number of examined and positive findings correlate with similar figures in Russia and have a two-wave pattern. Increased number of circulating immune complexes and activated T-lymphocytes is typical for all patients with new coronavirus infection COVID-19 regardless of age, which is a sign of acute infection-inflammatory process development and insufficiently effective elimination of antigen (pathogen).

scholarly journals CHARACTERISTICS OF SOME IMMUNOLOGICAL INDICATORS OF HIV INFECTION IN COMBINATION WITH TUBERCULOSIS

2021 ◽  
pp. 13-17
Author(s):  
V.D. Moskaliuk ◽  
T.R. Kolotylo
Keyword(s):  
Viral Load ◽  
T Lymphocytes ◽  
Hiv Infection ◽  
T Regulatory Cells ◽  
Interferon Γ ◽  
Interleukin 4 ◽  
Regulatory Cells ◽  
Suppressor Activity ◽  
Immunological Examination ◽  
Ifn Γ

The purpose of the work is to carry out a comparative analysis of epidemiological, clinical and individual laboratory parameters of groups of patients with HIV infection associated with tuberculosis (TB) and TB monoinfection. Patients and methods. A comprehensive immunological examination was performed on 231 patients, including 155 HIV-infected with active newly diagnosed tuberculosis and 76 on tuberculosis alone. The HIV/TB group was divided into 3 subgroups depending on the time of TB accession to HIV infection. The levels of interleukin-4 (IL-4) and interferon-γ (IFN-γ) were compared for groups with co-infection with HIV/TB and patients with TB monoinfection. Results. In associated HIV/TB infection, the level of CD4+T-lymphocytes is significantly lower compared to patients with TB monoinfection. In the HIV/TB group, it was established the presence of a medium feedback force between the number of CD4+T-lymphocytes and the serum concentration of IFN-γ (correlation coefficient r=-0.36, confidence level P<0.05); weak direct relationship between viral load and serum IFN-γ concentration (r=0.25, P<0.05); medium strength inverse relationship between the number of CD4+T-lymphocytes and the level of viral load (r=-0.44, P<0,01). In the group with TB monoinfection, no correlation was found between the number of CD4+T-lymphocytes and cytokine parameters. Conclusions. In associated HIV/TB infection, CD4+T-lymphocyte counts are significantly lower than in patients with TB only. As HIV infection progresses (decrease in CD4+T-lymphocytes and increase in HIV load), there is an increase in serum IFN-γ and IL-4, which probably indicates a decrease in the number of anti-inflammatory T-regulatory cells, or a decrease in their suppressor activity.

scholarly journals T Cell–dependent Immune Response in C1q-deficient Mice: Defective Interferon γ Production by Antigen-specific T Cells

1998 ◽  
Vol 187 (11) ◽  
pp. 1789-1797 ◽  
Author(s):  
Antony J. Cutler ◽  
Marina Botto ◽  
Dominic van Essen ◽  
Roberta Rivi ◽  
Kevin A. Davies ◽  
...  
Keyword(s):  
Immune Response ◽  
T Cells ◽  
T Cell ◽  
Keyhole Limpet Hemocyanin ◽  
Interferon Γ ◽  
Interleukin 4 ◽  
Classical Pathway ◽  
Classical Complement Pathway ◽  
Deficient Mice

The role of the classical complement pathway in humoral immune responses was investigated in gene-targeted C1q-deficient mice (C1qA−/−). Production of antigen-specific immunoglobulin (Ig)G2a and IgG3 in primary and secondary responses to T cell–dependent antigen was significantly reduced, whereas IgM, IgG1, and IgG2b responses were similar in control and C1qA−/− mice. Despite abnormal humoral responses, B cells from C1qA−/− mice proliferated normally to a number of stimuli in vitro. Immune complex localization to follicular dendritic cells within splenic follicles was lacking in C1qA−/− mice. The precursor frequency of antigen-specific T cells was similar in C1qA−/− and wild-type mice. However, analysis of cytokine production by primed T cells in response to keyhole limpet hemocyanin revealed a significant reduction in interferon-γ production in C1qA−/− mice compared with control mice, whereas interleukin 4 secretion was equivalent. These data suggest that the classical pathway of complement may influence the cytokine profile of antigen-specific T lymphocytes and the subsequent immune response.

The Presence and Clearance of Immune Complexes in the Untreated Generalised Psoriasis

1998 ◽  
Vol 11 (2) ◽  
pp. 91-99
Author(s):  
G. Ahangari ◽  
M. Rakhshan ◽  
A. Farhoudi ◽  
M. B. Eslami ◽  
H. Mortazavi ◽  
...  
Keyword(s):  
Reference Values ◽  
Lupus Erythematosus ◽  
Immune Complexes ◽  
Inflammatory Responses ◽  
Circulating Immune Complexes ◽  
Chromosome 1 ◽  
Complement C3 ◽  
Cell Swelling ◽  
Single Chain ◽  
Normal Ranges

Psoriasis is a hyperproliferative inflammatory disease and 70% of patients develop a chronic plaque form of the disease. The pathogenesis of psoriasis is not known but evidence exists that changes in micro vascular occur. There are micro vascular abnormalities in the capillaries which display a multilayer basement membrane with fenestration. Study of involved synovium in psoriatic arthritis reveals endothelial cell swelling, thickening of the vessel walls and inflammatory cell infiltration. Investigation on expression of CD35 molecules that clear the immune complexes were carried out in this study. CD35 is single chain glycoprotein (MW160–240 kD) and is located on the long arm of the chromosome 1. FACScan was used as laser flow cytometer. Initially 40 blood samples from normal individuals, 35 untreated Lupus Erythematosus Systemic and 35 Rheumatoid arthritis patients were studied as controls for reference values of CD35, Circulating immune Complexes (CIC), Complement C3 & C4. Next 34 patients suffering from psoriasis were studied for, Circulating immune Complexes (CIC), Complement C3 & C4. Comparison of these results with those of reference values normal ranges showed significant increase of CIC (P<0.05) and decrease of CD35 (P<0.001). Where as, the complement component levels C3 (P>0.05) and C4(P>0.05) indicates werenot significantly altered. This study shows that the decrease in expression of CD35 on Red blood cells in psoriasis patients is more crucial than C3 and C4 levels. Thus decrease of CD35 molecule in psoriasis may cause increased levels of CIC in patients' sera and promotion of inflammatory responses.

Determinants of the immune response in visceral leishmaniasis: Evidence for predominance of endogenous interleukin 4 over interferon-γ production

1990 ◽  
Vol 57 (2) ◽  
pp. 242-249 ◽  
Author(s):  
Kai Zwingenberger ◽  
Gundel Harms ◽  
Celia Pedrosa ◽  
Simone Omena ◽  
Beate Sandkamp ◽  
...  
Keyword(s):  
Immune Response ◽  
Visceral Leishmaniasis ◽  
Interferon Γ ◽  
Interleukin 4

scholarly journals The impact of immune disturbances on the failure of antituberculosis treatment

2016 ◽  
Vol 89 (4) ◽  
pp. 493-498 ◽  
Author(s):  
Evelina Lesnic ◽  
Serghei Ghinda ◽  
Carmen Monica Pop
Keyword(s):  
Immune Response ◽  
T Cell ◽  
Pulmonary Tuberculosis ◽  
Treatment Failure ◽  
Humoral Immunity ◽  
Cellular Immunity ◽  
Immune Complexes ◽  
Treatment Initiation ◽  
Circulating Immune Complexes ◽  
Antituberculosis Treatment

Background and aim. Tuberculosis is an infectious disease caused by Mycobacterium tuberculosis complex, with an evolution and treatment outcome determined by the interaction between the mycobacterial and human genotypes. Various deficiencies of innate immune response starting from the first encounter of M. tuberculosis with lung cells endanger host infection control due to decreased triggering of cellular immune resistance and disturbed humoral immunity. Disturbed cell mediated immunity, known as the basic immune response in tuberculous infection, contributes to the deficient generation of central necrosis granuloma, consequently being responsible for severe clinical aspects and low final outcome. The tuberculosis patient’s immune assessment is important before treatment initiation, for establishing the risk reduction measures and increasing success rate.Material and methods. The immune study was conducted on 54 new pulmonary tuberculosis cases with treatment failure, 34 new pulmonary tuberculosis cases that successfully ended the treatment and 50 healthy group individuals. Immune assays performed were: blastic transformation of lymphocytes induced by different antigens, quantitatitve assessment of cellular immunity through CD4+ T cell and CD8+ T cell phenotyping, humoral immunity - through immunoglobulin isotyping, innate resistance – through phagocyte activity of neutrophils, the titter of anti-tuberculosis antibodies and the serum level of circulating immune complexes. Investigations were performed at the onset the treatment and at the end of intensive phase of the standard anti-tuberculosis treatment.Results. Immune disturbances evidenced in patients with treatment failure were: important deficiencies of cellular immunity, hyperactivity of humoral immunity and deficiencies of innate immunity. High predictive value for treatment failure showed the indices: deficiency of T lymphocytes count (OR=62.5) and T helper count (OR=12.5), high level of circulating immune complexes (OR=9.801), deficiency of innate resistance (decreased phagocytating index OR=2.875).Conclusions. For increasing the treatment success rate, the study of immune disturbances must be performed before of antituberculosis treatment initiation , especially of cellular immunity for the early start of immune adaptive treatment.

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