Preparation of Curcumin Nanoparticles and Cellular Uptake Study on HeLa Cells

Objective: This study aimed to evaluate the effectivity of resveratrol-gold-PEG-folate (RSV-Au-PEG-FA) nanoparticles formulation in resveratrol (RSV) targeted delivery and cytotoxicity effect on HeLa human cervical cancer cell line. Methods: Gold nanoparticles (AuNP) were used as carriers and folic acid (FA) was used as active targeting moiety, using polyethylene glycol-bis-amine (PEG-bis-amine) as linker. RSV-Au-PEG-FA nanoparticles were characterized by UV-Vis spectrophotometry, infrared spectroscopy, particle size analyzer (PSA), and transmission electron microscopy (TEM). Cellular uptake study was conducted by using fluorescence microscope. Cytotoxicity study was conducted by 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Results: Cellular uptake study has shown that RSV-Au-PEG-FA nanoparticles are potential to be accumulated intracellularly in HeLa cells more than in Vero cells. Cytotoxicity study has shown RSV-Au-PEG-FA nanoparticles IC50 67.06±2.14 mM and RSV IC50 9.66±1.44 mM on HeLa cells Conclusion: RSV-Au-PEG-FA nanoparticles are potential to enhance RSV uptake by HeLa cells selectively.

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Recent Advances in Curcumin Nanocarriers for the Treatment of Different Types of Cancer with Special Emphasis on In Vitro Cytotoxicity and Cellular Uptake Studies

Nanoscience &Nanotechnology-Asia ◽

10.2174/2210681209666190417144126 ◽

2020 ◽

Vol 10 (5) ◽

pp. 577-590

Author(s):

Jai B. Sharma ◽

Shailendra Bhatt ◽

Asmita Sharma ◽

Manish Kumar

Keyword(s):

Cancer Cells ◽

Cellular Uptake ◽

Anticancer Agents ◽

Targeted Delivery ◽

In Vitro Cytotoxicity ◽

Curcumin Nanoparticles ◽

Cytotoxicity Studies ◽

Delivery Technique ◽

Different Types

Background: The potential use of nanocarriers is being explored rapidly for the targeted delivery of anticancer agents. Curcumin is a natural polyphenolic compound obtained from rhizomes of turmeric, belongs to family Zingiberaceae. It possesses chemopreventive and chemotherapeutic activity with low toxicity in almost all types of cancer. The low solubility and bioavailability of curcumin make it unable to use for the clinical purpose. The necessity of an effective strategy to overcome the limitations of curcumin is responsible for the development of its nanocarriers. Objective: This study is aimed to review the role of curcumin nanocarriers for the treatment of cancer with special emphasis on cellular uptake and in vitro cytotoxicity studies. In addition to this, the effect of various ligand conjugated curcumin nanoparticles on different types of cancer was also studied. Methods: A systematic review was conducted by extensively surfing the PubMed, science direct and other portals to get the latest update on recent development in nanocarriers of curcumin. Results: The current data from recent studies showed that nanocarriers of curcumin resulted in the targeted delivery, higher efficacy, enhanced bioavailability and lower toxicity. The curcumin nanoparticles showed significant inhibitory effects on cancer cells as compared to free curcumin. Conclusion: It can be concluded that bioavailability of curcumin and its cytotoxic effect to cancer cells can be enhanced by the development of curcumin based nanocarriers and it was found to be a potential drug delivery technique for the treatment of cancer.

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Cellular Uptake Study of Antimycotic-Loaded Carriers Using Imaging Flow Cytometry and Confocal Laser Scanning Microscopy

Optics and Spectroscopy ◽

10.1134/s0030400x20060235 ◽

2020 ◽

Vol 128 (6) ◽

pp. 799-808

Author(s):

R. A. Verkhovskii ◽

E. V. Lengert ◽

M. S. Saveleva ◽

A. A. Kozlova ◽

V. V. Tuchin ◽

...

Keyword(s):

Flow Cytometry ◽

Confocal Laser Scanning Microscopy ◽

Cellular Uptake ◽

Laser Scanning ◽

Laser Scanning Microscopy ◽

Confocal Laser Scanning ◽

Scanning Microscopy ◽

Confocal Laser ◽

Uptake Study ◽

Imaging Flow Cytometry

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Protein-Coated Aryl Modified Gold Nanoparticles for Cellular Uptake Study by Osteosarcoma Cancer Cells

10.26434/chemrxiv.12296261 ◽

2020 ◽

Author(s):

Mehvesh Hameed ◽

Seema Panicker ◽

Sallam Hasan Abdallah ◽

Amir A. Khan ◽

Changseok Han ◽

...

Keyword(s):

Gold Nanoparticles ◽

Cancer Cells ◽

Cellular Uptake ◽

Phase Transfer ◽

Chemical Reduction ◽

Uptake Study ◽

Stabilizing Agents ◽

Coated Nanoparticles ◽

Green Route ◽

The Difference

We synthesized protein-coated gold nanoparticles using green and chemical reduction routes for cellular uptake study. In the current work, we coated gold-aryl nanoparticles of the type AuNPs-C<sub>6</sub>H<sub>4</sub>-4-COOH with BSA, collagen, zein and lysozyme proteins. Both routes were carried out without phase-transfer catalysts or extraneous stabilizing agents. High crystallinity of the AuNPs synthesized by the green route can be seen in the transmission electron microscopy images. <a>Osteosarcoma cancer cells are malignant bone tumors with abnormal cellular functions. Studies using MG-63 cells will provide mechanistic suggestions on the details of the amplification in tumors. </a>We studied the cellular uptake of the bioconjugates by MG-63 osteosarcoma cells using laser confocal fluorescence microscopy (LCFM) and flow cytometry. In the LCFM study, BSA-AuNPs was uptaken most efficiently of all protein-coated gold nanoparticles synthesized by the green route. Zein and lysozyme coated nanoparticles, though small sizes, prepared by the green method were not efficiently uptaken by MG-63. The two nanoparticles are negatively charged and zein is also a hydrophobic coat. The difference in hydrophobicity and charge might have affected the internalization. All of those coated nanoparticles that were efficiently uptaken can potentially be used as diagnostic and therapeutic agents for osteosarcoma.

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Membrane Microdomain Structures of Liposomes and Their Contribution to the Cellular Uptake Efficiency into HeLa Cells

Molecular Pharmaceutics ◽

10.1021/acs.molpharmaceut.5b00601 ◽

2016 ◽

Vol 13 (2) ◽

pp. 369-378 ◽

Cited By ~ 8

Author(s):

Yoshinori Onuki ◽

Yasuko Obata ◽

Kumi Kawano ◽

Hiromu Sano ◽

Reina Matsumoto ◽

...

Keyword(s):

Cellular Uptake ◽

Hela Cells ◽

Uptake Efficiency ◽

Membrane Microdomain ◽

Cellular Uptake Efficiency

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Cellular Uptake and Cytotoxicity of Varying Aspect Ratios of Gold Nanorods in HeLa Cells

ACS Applied Bio Materials ◽

10.1021/acsabm.9b00986 ◽

2020 ◽

Vol 3 (3) ◽

pp. 1374-1384 ◽

Cited By ~ 1

Author(s):

Deshani Fernando ◽

Shoukath Sulthana ◽

Yolanda Vasquez

Keyword(s):

Cellular Uptake ◽

Gold Nanorods ◽

Hela Cells ◽

Aspect Ratios

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Evaluation of Chitosan-Tripolyphosphate Nanoparticles as a p-shRNA Delivery Vector: Formulation, Optimization and Cellular Uptake Study

Journal of Nanopharmaceutics and Drug Delivery ◽

10.1166/jnd.2013.1027 ◽

2013 ◽

Vol 1 (3) ◽

pp. 266-278 ◽

Cited By ~ 16

Author(s):

Mahdi Karimi ◽

Pinar Avci ◽

Mohsen Ahi ◽

Tarane Gazori ◽

Michael R. Hamblin ◽

...

Keyword(s):

Cellular Uptake ◽

Uptake Study ◽

Formulation Optimization ◽

Delivery Vector

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Preparation and In Vitro Cellular Uptake Assessment of Multifunctional Rubik-Like Magnetic Nano-Assemblies

Journal of Nanoscience and Nanotechnology ◽

10.1166/jnn.2019.16129 ◽

2019 ◽

Vol 19 (6) ◽

pp. 3301-3309

Author(s):

Xiawen Zheng ◽

Yuejian Chen ◽

Zhiming Wang ◽

Lina Song ◽

Yu Zhang ◽

...

Keyword(s):

Oleic Acid ◽

Folic Acid ◽

Cellular Uptake ◽

Hela Cells ◽

Drug Loading ◽

Superparamagnetic Iron Oxide ◽

Drug Carriers ◽

High Drug ◽

High Drug Loading

Through self-assembly of nanoparticles into high-order and stable structures of cubic clusters, high drug-loading rubik-like magnetic nano-assemblies (MNAs), possessing folic acid targeting and strong magnetism-enhanced cellular uptake capabilities, were built. In this study, the core of the cubic drug assemblies consisted of four monodisperse superparamagnetic iron oxide nanoparticles coated with layers of oleic acid (Fe3O4@OA), simultaneously encapsulating fluorescein, and Paclitaxol (Flu-MNAs and PTX-MNAs) for imaging and therapeutic applications. To enable preferential tumor cellular uptake by the nanocarriers, the outermost layer of Fe3O4 was functionalized with the new dual-oleic acid-polyethylene glycol-folic acid polymer (FA-PEG-Lys-OA2) as a “shell.” The drug carriers exhibited excellent stability and biocompatibility, and showed high drug loading and excellent magnetic response In Vitro. Furthermore, preliminary evaluations of the drug carriers with Hela cells showed effective cellular targeting capability. In addition, the cubic assemblies enhanced anticancer efficiency for Hela cells compared to bare drugs. Especially, the applied external magnetic field further improved the uptake of the vectors, and thereby enhanced the inhibitory effect. In brief, all these results suggested that cubic assemblies could serve as potential strategies for targeted anticancer therapies.

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Protein-Coated Aryl Modified Gold Nanoparticles for Cellular Uptake Study by Osteosarcoma Cancer Cells

Langmuir ◽

10.1021/acs.langmuir.0c01443 ◽

2020 ◽

Vol 36 (40) ◽

pp. 11765-11775

Author(s):

Mehavesh Hameed ◽

Seema Panicker ◽

Sallam H. Abdallah ◽

Amir A. Khan ◽

Changseok Han ◽

...

Keyword(s):

Gold Nanoparticles ◽

Cancer Cells ◽

Cellular Uptake ◽

Uptake Study

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Identification of Porphyrin-Silica Composite Nanoparticles using Atmospheric Solids Analysis Probe Mass Spectrometry

MRS Advances ◽

10.1557/adv.2019.217 ◽

2019 ◽

Vol 4 (38-39) ◽

pp. 2079-2086

Author(s):

Casey Karler ◽

Kylea J. Parchert ◽

James B. Ricken ◽

Bryan Carson ◽

Curtis D. Mowry ◽

...

Keyword(s):

Mass Spectrometry ◽

Cellular Uptake ◽

Hela Cells ◽

Energy Transduction ◽

Composite Nanoparticles ◽

Growth Media ◽

Glass Capillary ◽

Silica Composite ◽

Novel Method ◽

Solids Analysis

ABSTRACTPorphyrins are vital pigments involved in biological energy transduction processes. Their abilities to absorb light, then convert it to energy, have raised the interest of using porphyrin nanoparticles as photosensitizers in photodynamic therapy. A recent study showed that self- assembled porphyrin-silica composite nanoparticles can selectively destroy tumor cells, but detection of the cellular uptake of porphyrin-silica composite nanoparticles was limited to imaging microscopy. Here we developed a novel method to rapidly identify porphyrin-silica composite nanoparticles using Atmospheric Solids Analysis Probe-Mass Spectrometry (ASAP-MS). ASAP-MS can directly analyze complex mixtures without the need for sample preparation. Porphyrin-silica composite nanoparticles were vaporized using heated nitrogen desolvation gas, and their thermo-profiles were examined to identify distinct mass- to-charge (M/Z) signatures. HeLa cells were incubated in growth media containing the nanoparticles, and after sufficient washing to remove residual nanoparticles, the cell suspension was loaded onto the end of ASAP glass capillary probe. Upon heating, HeLa cells were degraded and porphyrin-silica composite nanoparticles were released. Vaporized nanoparticles were ionized and detected by MS. The cellular uptake of porphyrin-silica composite nanoparticles was identified using this ASAP-MS method.

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