Protein Expression Changes Induced by 5-Fluorouracil in an Oral Cancer Cell Line as Determined by Immunoprecipitation High Performance Liquid Chromatography

Background: Cancer is rapidly evolving life-threatening ailment in the mankind due to changes in daily food intake and lifestyle changes. Oral carcinoma is 6th major cause of cancer death in the world and it is third major reason of cancer mortality in India. Every cell in the human body requires glucose for its metabolic energy. Besides normal cell, cancer cells also require the glucose for its endurance and multiplication. SGLT2 inhibitors which are aimed at diabetes therapy exhibited anticancer properties also in colon and pancreatic cancer lines. Present study aim is to evaluate the anticancer activity of SGLT2 inhibitors against oral cancer cell by MTT Assay.Methods: To evaluate the anticancer activity of SGLT2 inhibitors MTT Cytotoxic assay is performed as per standard protocols. Cancer cells were plated in 24-well plates and incubated at 370C with 5% CO2 condition. After convergence, samples are added to the plates in various concentrations and allowed to incubate then they are detached from the plates and cleansed with the reagents. The wells are coated with the dye and incubated. Later samples are analysed in UV-spectrophotometer.Results: Cytotoxic assay showed decrease in cell viability with increasing dose of SGLT2 inhibitors. IC50 values were determined graphically. The IC50 value of dapagliflozin is 400µg/ml and canagliflozin is 250µg/ml respectively after 24 hours of Assessment.Conclusions: The results of the current study give us an evidence that SGLT2 inhibitors dapagliflozin and canagliflozin exhibits anticancer property in Oral Cancer cell line.

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Experimental chemotherapy using a human oral floor cancer transplanted to nude mice. 1. Establishment of a oral cancer cell line in nude mice.

Japanese Journal of Oral & Maxillofacial Surgery ◽

10.5794/jjoms.33.1130 ◽

1987 ◽

Vol 33 (6) ◽

pp. 1130-1136

Author(s):

Eisuke TOMIYA ◽

Arihide MOCHIZUKI ◽

Noboru HAYASHI ◽

Takeshi HONDA ◽

Katsuma KOMOTO

Keyword(s):

Oral Cancer ◽

Cell Line ◽

Cancer Cell ◽

Nude Mice ◽

Cancer Cell Line ◽

Experimental Chemotherapy ◽

Oral Cancer Cell ◽

Oral Cancer Cell Line

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PRODUCTION OF CAMPTOTHECIN FROM ENDOPHYTIC FUNGI AND CHARACTERIZATION BY HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY AND ANTICANCER ACTIVITY AGAINST COLON CANCER CELL LINE.

Asian Journal of Pharmaceutical and Clinical Research ◽

10.22159/ajpcr.2018.v11i3.18921 ◽

2018 ◽

Vol 11 (3) ◽

pp. 166 ◽

Cited By ~ 2

Author(s):

Aswini A ◽

Soundhari C

Keyword(s):

Colon Cancer ◽

High Performance Liquid Chromatography ◽

Liquid Chromatography ◽

Cell Line ◽

Cancer Cell ◽

High Performance ◽

Cancer Cell Line ◽

Colon Cancer Cell Line ◽

Colon Cancer Cell ◽

Biologically Active

Objective: Scientists are showing increasing interest in studying fungal endophytes as sources of novel and biologically active compounds. The present study was attempted to isolate and characterize camptothecin (CPT) from endophytic fungus Aspergillus niger isolated from Indian Piper betel plant and detection of its anticancer potential on colon cancer cell line.Methods: The production of CPT was confirmed by high-performance liquid chromatography techniques. The effect of CPT on hematopoietic cell transplantation (HCT) colon cancer cell line was studied by MTT assay.Results: The amount of CPT which is isolated from A. niger was found to be around 0.175 mg/L. Significant death of HCT cells was observed and was non-toxic to normal vero cell line.Conclusion: Hence, CPT can be used as a potential lead compound in cancer research.

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