A influência da irradiação ultrassônica de baixa intensidade em cultura de células fibroblásticas

2015 ◽  
Vol 13 ◽  
pp. 306
Author(s):  
Larissa Dragonetti Bertin ◽  
Deise Aparecida Almeida Pires-Oliveira ◽  
Priscila Daniela Oliveira ◽  
Juliana Almeida Serpeloni ◽  
Stheace Kelly Fernandes Szezerbaty ◽  
...  
Keyword(s):  
Duty Cycle ◽  
Healing Process ◽  
Control Group ◽  
Mitochondrial Activity ◽  
Fibroblast Cells ◽  
Pulse Regime ◽  
Pulsed Ultrasound ◽  
Low Intensity Pulsed Ultrasound ◽  
Low Intensity

Introduction: In vitro and in vivo put in evidence that the Low Intensity Pulsed Ultrasound therapy exerts a significant influence on cell function (cytoskeleton organization, stimulation of mitochondrial activity, ATP levels and plasma membrane). Objective: This study will analyze the radiation of low intensity pulsed ultrasound in fibroblast cells L 929. Method: In this study are presented the data from each exposure group average and standard deviation in each moment of evaluation (24 hours, 48 hours and 72 hours). The control group (received no radiation), 0.2 W/cm2 with 10% pulse regime (1: 9 duty cycle), 0.2 W / cm2 with 20% pulse regime (2: 8 cycle work), 0.4 W/cm2 with pulse scheme 10% (1: 9 duty cycle), 0.4 W/cm2 with pulse scheme 20% (2: 8 duty cycle). The analyzes will be performed through optical microscopy, MTT method 3 - (4,5-dimethylthiazol-2-yl) -2,5 diphenyl tetrazolium bromide, within the incubation times of 24, 48 and 72 hours. Results: Given the above study, the results presented in this project will be directed to increase the stimulation process and proliferation of fibroblast cells from the pulsed ultrasonic irradiation of low intensity, correlating with the healing process, neovascularization and repair. Conclusion: Therefore, the study of the effect of ultrasound from cell culture provides us with a simple and informative model on the significant aspects of the use of physical therapy in vivo system.

scholarly journals The Effect of Low Intensity Pulsed Ultrasound on Endometrial Receptivity in Infertile Patients with Adenomyosis

2022 ◽  
Author(s):  
Ling Long ◽  
Ling Zhou ◽  
Jing Zhu ◽  
Xuan He
Keyword(s):  
Western Blotting ◽  
Endometrial Receptivity ◽  
Control Group ◽  
Pulsed Ultrasound ◽  
Expression Levels ◽  
Low Intensity Pulsed Ultrasound ◽  
Low Intensity ◽  
Infertile Patients

Abstract Objective: Adenomyosis (AM) is an important cause of female infertility, and its disease mechanism remains unclear. This study preliminarily investigated the expression of endometrial receptivity markers homeobox A10 (HOXA10) and leukemia inhibitory factor (LIF) in infertile patients with AM and described the effects of low intensity pulsed ultrasound (LIPUS) on it. Methods: In vivo, tissues were obtained from the infertile female AM patient group (AG group, n=10) and healthy control group (CG group, n=11). The expression of HOXA10 and LIF in the two groups was detected by immunohistochemistry (IHC) and western blotting. In vitro, primary cells were extracted and cultured from the two groups, and the expression of HOXA10 and LIF protein was detected by western blotting. Then the AG cells were treated with 15, 30, and 60 mW/cm2 of LIPUS for 7 days (20 min/day), and detected the cell adhesion rate. Finally, treat the AG cells with 30mW/cm2 LIPUS for 7 days (20 min/day), and detect the expression level of ICAM-1 in the cell supernatant by ELISA. The AG cells was treated with 30 mW/cm2 LIPUS for 4 days (20 min/day), and the expression levels of HOXA10 and LIF were detected by western blotting, RT-PCR, and agarose gel electrophoresis. Results: In vivo, IHC staining showed that HOXA10 and LIF proteins were mainly localized in endometrial epithelial cells. Both IHC and western blot showed that the levels of HOXA10 and LIF in the AG group were significantly lower than those in the CG group (P<0.01, P<0.05). In vitro, the expression levels of HOXA10 and LIF protein in the AG cell was significantly lower than those in the CG cell (P<0.001). Then, the cell adhesion ability of the 30 and 60 mW/cm2 groups was higher than that of the 15 mW/cm2 group after LIPUS treatment. Finally, The concentration of ICAM-1 in the supernatant of AG cells treated with LIPUS was significantly higher than that of the control group (P<0.01), and the AG cells were treated with 30 mW/cm2 LIPUS for 4 days (20 min/day), the protein and mRNA expression levels of HOXA10 and LIF were higher than those of the control group (P<0.001). Conclusion: The reduction of HOXA10 and LIF may be one of the reasons for the decreased endometrial receptivity in AM. The LIPUS promoted the adhesion and the expression of HOXA10 and LIF of EEECs from the AM group, thereby increasing endometrial receptivity.

scholarly journals Osteocytes as main responders to low-intensity pulsed ultrasound treatment during fracture healing

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Tatsuya Shimizu ◽  
Naomasa Fujita ◽  
Kiyomi Tsuji-Tamura ◽  
Yoshimasa Kitagawa ◽  
Toshiaki Fujisawa ◽  
...  
Keyword(s):  
Fracture Healing ◽  
Transcriptional Control ◽  
Target Genes ◽  
Pulsed Ultrasound ◽  
Activated T Cells ◽  
In Vivo Models ◽  
Low Intensity Pulsed Ultrasound ◽  
Low Intensity ◽  
Ultrasound Stimulation

AbstractUltrasound stimulation is a type of mechanical stress, and low-intensity pulsed ultrasound (LIPUS) devices have been used clinically to promote fracture healing. However, it remains unclear which skeletal cells, in particular osteocytes or osteoblasts, primarily respond to LIPUS stimulation and how they contribute to fracture healing. To examine this, we utilized medaka, whose bone lacks osteocytes, and zebrafish, whose bone has osteocytes, as in vivo models. Fracture healing was accelerated by ultrasound stimulation in zebrafish, but not in medaka. To examine the molecular events induced by LIPUS stimulation in osteocytes, we performed RNA sequencing of a murine osteocytic cell line exposed to LIPUS. 179 genes reacted to LIPUS stimulation, and functional cluster analysis identified among them several molecular signatures related to immunity, secretion, and transcription. Notably, most of the isolated transcription-related genes were also modulated by LIPUS in vivo in zebrafish. However, expression levels of early growth response protein 1 and 2 (Egr1, 2), JunB, forkhead box Q1 (FoxQ1), and nuclear factor of activated T cells c1 (NFATc1) were not altered by LIPUS in medaka, suggesting that these genes are key transcriptional regulators of LIPUS-dependent fracture healing via osteocytes. We therefore show that bone-embedded osteocytes are necessary for LIPUS-induced promotion of fracture healing via transcriptional control of target genes, which presumably activates neighboring cells involved in fracture healing processes.

scholarly journals Low-Intensity Pulsed Ultrasound Promotes Autophagy-Mediated Migration of Mesenchymal Stem Cells and Cartilage Repair

2021 ◽  
Vol 30 ◽  
pp. 096368972098614
Author(s):  
Peng Xia ◽  
Xinwei Wang ◽  
Qi Wang ◽  
Xiaoju Wang ◽  
Qiang Lin ◽  
...  
Keyword(s):  
Cartilage Repair ◽  
Matrix Protein ◽  
Extracellular Matrix Protein ◽  
Histopathological Analysis ◽  
Pulsed Ultrasound ◽  
Low Intensity Pulsed Ultrasound ◽  
Low Intensity ◽  
Autophagy Inhibitor

Mesenchymal stem cell (MSC) migration is promoted by low-intensity pulsed ultrasound (LIPUS), but its mechanism is unclear. Since autophagy is known to regulate cell migration, our study aimed to investigate if LIPUS promotes the migration of MSCs via autophagy regulation. We also aimed to investigate the effects of intra-articular injection of MSCs following LIPUS stimulation on osteoarthritis (OA) cartilage. For the in vitro study, rat bone marrow-derived MSCs were treated with an autophagy inhibitor or agonist, and then they were stimulated by LIPUS. Migration of MSCs was detected by transwell migration assays, and stromal cell-derived factor-1 (SDF-1) and C-X-C chemokine receptor type 4 (CXCR4) protein levels were quantified. For the in vivo study, a rat knee OA model was generated and treated with LIPUS after an intra-articular injection of MSCs with autophagy inhibitor added. The cartilage repair was assessed by histopathological analysis and extracellular matrix protein expression. The in vitro results suggest that LIPUS increased the expression of SDF-1 and CXCR4, and it promoted MSC migration. These effects were inhibited and enhanced by autophagy inhibitor and agonist, respectively. The in vivo results demonstrate that LIPUS significantly enhanced the cartilage repair effects of MSCs on OA, but these effects were blocked by autophagy inhibitor. Our results suggest that the migration of MSCs was enhanced by LIPUS through the activation autophagy, and LIPUS improved the protective effect of MSCs on OA cartilage via autophagy regulation.

Low-Intensity Pulsed Ultrasound (LIPUS) May Prevent Polyethylene Induced Periprosthetic Osteolysis In Vivo

2012 ◽  
Vol 38 (2) ◽  
pp. 238-246 ◽  
Author(s):  
Xiang Zhao ◽  
Xun-Zi Cai ◽  
Zhong-Li Shi ◽  
Fang-Bing Zhu ◽  
Gang-Sheng Zhao ◽  
...  
Keyword(s):  
Pulsed Ultrasound ◽  
Periprosthetic Osteolysis ◽  
Low Intensity Pulsed Ultrasound ◽  
Low Intensity

scholarly journals Histological and Radiological Evaluation of Low-Intensity Pulsed Ultrasound Versus Whole Body Vibration on Healing of Mandibular Bone Defects in Rats

Medicina ◽  
2020 ◽  
Vol 56 (9) ◽  
pp. 457
Author(s):  
Milad Etemadi Sh ◽  
Nan-Chen Hsieh ◽  
Seyed Shahin Movahed Mohammadi ◽  
Shahrooz Momeni ◽  
Seyed Mohammad Razavi ◽  
...  
Keyword(s):  
Bone Density ◽  
Whole Body Vibration ◽  
Bone Defects ◽  
Whole Body ◽  
Control Group ◽  
Pulsed Ultrasound ◽  
Mandibular Bone ◽  
Body Vibration ◽  
Low Intensity Pulsed Ultrasound ◽  
Low Intensity

Background and Objectives: Mechanical stimulation can improve the structural properties of the fracture site and induce the differentiation of different cell types for bone regeneration. This study aimed to compare the effect of low-intensity pulsed ultrasound stimulation (LIPUS) versus whole body vibration (WBV) on healing of mandibular bone defects. Materials and Methods: A mandibular defect was created in 66 rats. The rats were randomly divided into two groups of rats. Each group was subdivided randomly by three groups (n = 11) as follows: (I) control group, (II) treatment with LIPUS, and (III) treatment with WBV. The radiographic changes in bone density, the ratio of lamellar bone to the entire bone volume, the ratio of the newly formed bone to the connective tissue and inflammation grade were evaluated after 1 and 2 months. Results: LIPUS significantly increased the radiographic bone density change compared to the control group at the first and second month postoperatively (p < 0.01). WBV only significantly increased the bone density compared to the control group at the second month after the surgery (p < 0.01). Conclusions: Application of LIPUS and WBV may enhance the regeneration of mandibular bone defects in rats. Although LIPUS and WBV are effective in mandibular bone healing, the effects of LIPUS are faster and greater than WBV.

scholarly journals 174 THE EFFECT OF LOW-INTENSITY PULSED ULTRASOUND FOR SACFFOLD-FREE CHONDROCYTE PLATE IN VITRO AND IN VIVO

2009 ◽  
Vol 17 ◽  
pp. S102-S103
Author(s):  
K. Uenaka ◽  
S. Imai ◽  
S. Shioji ◽  
K. Kumagai ◽  
N. Okumura ◽  
...  
Keyword(s):  
Pulsed Ultrasound ◽  
Low Intensity Pulsed Ultrasound ◽  
Low Intensity

scholarly journals Effects of low intensity pulsed ultrasound to reduce the effusion volumes and pain with knee osteoarthritis: a randomized controlled trial

2018 ◽  
Vol 5 (1) ◽  
pp. 77
Author(s):  
P. Shanmuga Raju ◽  
Chokkarapu Ramu ◽  
N. S. Harshavardhan ◽  
K. Rajender ◽  
G. Sachin
Keyword(s):  
Randomized Controlled Trial ◽  
Knee Osteoarthritis ◽  
Controlled Trial ◽  
Control Group ◽  
Ultrasound Therapy ◽  
Pulsed Ultrasound ◽  
Low Intensity Pulsed Ultrasound ◽  
Low Intensity ◽  
Randomized Controlled ◽  
And Control

<p class="abstract"><strong>Background:</strong> The study aim was to determine the effect of low intensity pulsed ultrasound therapy to reduce the effusion volumes and pain in patients with Knee OA.</p><p class="abstract"><strong>Methods:</strong> This study design was randomized controlled trial. Total 50 patients diagnosed with Knee osteoarthritis were randomly assigned to two groups. Group I was using treatment of low intensity pulsed ultrasound therapy and group II was administered TENS with home exercise respectively. Treatments were 6 days per week and duration of 2 week. The amount of effusion volume will be measured via ultrasonograpy in knee.<strong></strong></p><p class="abstract"><strong>Results:</strong> The<strong> </strong>maximum number of cases are lying in age group &gt;60 years which is 28% and 40% in cases and control group respectively and age distribution in both the group is statistically not significant. The mean age of patients in cases and control group is 57.08±7.40 years and 58.04±9.93 years respectively.</p><p class="abstract"><strong>Conclusions:</strong> Low intensity pulsed ultrasound therapy significantly reduced the effusion volumes and pain in patients with knee osteoarthritis.</p>

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