Effect of Various Concentrations of Sodium Hypochlorite on Primary Dentin: An in vitro Scanning Electron Microscopic Study

2012 ◽  
Vol 37 (1) ◽  
pp. 37-43 ◽  
Author(s):  
L Gowda ◽  
U Mohan Das
Keyword(s):  
Sodium Hypochlorite ◽  
In Vitro Study ◽  
Complete Removal ◽  
Collagen Fibrils ◽  
Smear Layer ◽  
Control Group ◽  
Electron Microscopic ◽  
Time Of Application ◽  
Anterior Teeth

Background: Sodium hypochlorite solutions have been evaluated for their effects in bonding procedures as they are found to deplete or remove the organic portion of the dentin, particularly the collagen fibrils. The aim of this in vitro study was to assess and compare the efficacies of 1%, 2.5%, 5% and 10% NaOCl at 30, 60 and 120s on etched primary dentin. Methods: 84 primary anterior teeth were ground to expose a flat dentin area on the buccal surface. The specimens were divided into fourteen groups of six each based on the dentin surface treatment (35% phosphoric acid etching for 7 seconds-AE and/or NaOCl application), NaOCl solution concentrations (1%, 2.5%, 5% and 10%) and time of application (0, 30, 60 and 120s). Specimens were prepared for SEM and photomicrographs were taken of the surface and were scored against a five point scale, based on the smear layer and amount of collagen removed. The scores were submitted to Kruskal-Wallis and Mann Whitney tests. Results: This study showed the presence of smear layer in the control group. The group treated with Acid Etchant showed a demineralized pattern of dentin with exposure of dentin tubules and collagen fibrils network on the intertubular and peritubular dentin which was not significantly different from the groups treated with 1% and 2.5% NaOCl. Groups treated with 5% NaOCl were not statistically different from each other, the surface was corroded but collagen fibrils were not completely depleted. Groups treated with 10% NaOCl were not statistically different from each other and showed complete removal of collagen fibrils with wider tubular apertures and several secondary tubules on peritubular and intertubular dentin. Conclusion: Higher concentrations of NaOCl solutions (5% and 10%) produced significant changes in the etched primary dentin. The higher the concentration of the NaOCl solution, the lower can be the time for the application of the solution for the complete removal of collagen fibrils.

Comparative Study to Evaluate the Surface Detail Reproduction of Dental Stone after Immersion in Various Different Disinfectant Solutions, Under Stereomicroscope 10 X Magnification –An In-Vitro Study

2017 ◽  
Vol 9 (3) ◽  
Author(s):  
Rathika Rai ◽  
M. A. Easwaran ◽  
K. T. Dhivya
Keyword(s):  
Sodium Hypochlorite ◽  
In Vitro Study ◽  
Control Group ◽  
Immersion Method ◽  
Significant Difference ◽  
Surface Irregularities ◽  
Disinfectant Solution ◽  
Surface Detail ◽  
Group B

Aim: To evaluate the surface detail reproduction of dental stone this is immersed in different disinfectant solution and studied under stereomicroscope. Methodology: Total number of 30 specimens of dental stone (Type III) were made with measurements of 1.5cm diameter and 1cm height .This samples are divided in to 3 groups group A,B,C. were A is immersed in Distilled water which was taken as control group ;B is immersed in 2% Glutaraldehyde and C is immersed in 5%sodium hypochlorite. Each specimen were immersed in the disinfectant solution for 15 minutes and dried under room temperature for 24 hrs. After 24 hrs each specimens are studied under stereomicroscope for surface details. Result: The results showed no significant difference in the surface irregularities and porosities for a group 1 and group 2 except group 3 which showed significant increase in the porosities, surface irregularities and erosions after disinfection with 5% NaHOCl by immersion method. Conclusion: The surface detail reproduction capacity of die stone was adversely affected when 5% Sodium hypochlorite was used as disinfectant solution when compare d to control group and 2% Glutaraldehyde

scholarly journals Effect of Incorporation of Disinfectants on Surface Hardness and Dimensional Accuracy of Die Stone: An in vitro Study

2017 ◽  
Vol 7 (3) ◽  
pp. 86-91
Author(s):  
Anubhuti Bakshi ◽  
Rajat Dang
Keyword(s):  
Sodium Hypochlorite ◽  
Testing Machine ◽  
Surface Hardness ◽  
In Vitro Study ◽  
Dimensional Accuracy ◽  
Control Group ◽  
Time Interval ◽  
Type Iv ◽  
Post Hoc

ABSTRACT Aim To evaluate surface hardness and dimensional accuracy of type IV dental stone after incorporation of disinfectants. Materials and methods A total of 100 specimens, 50 (40 mm⨯ 10 mm) disk shaped and 50 frustum cone shaped, were fab- ricated from type IV dental stone after incorporation of various disinfectants, namely glutaraldehyde (2%), iodophor (1.76%), chlorhexidine (2%), and sodium hypochlorite (1%), for evaluation of surface hardness and dimensional accuracy. Surface hardness was tested after 1- and 24-hour time interval using Rockwell hard- ness testing machine. Dimensional accuracy was measured using traveling microscope. Data obtained were compiled and analyzed statistically by using analysis of variance and post hoc test. Results Results showed significant decrease in surface hard- ness of samples incorporated with disinfectants at 1 hour as compared with control (distilled water). Surface hardness of all the samples increased with time and was almost equal to control group at 24-hour interval, with iodophor showing the maximum value in comparison with the other disinfectants used. When dimensional accuracy of all the groups was compared, iodophor and sodium hypochlorite were found to cause negative alterations. Conclusion Within the limitations of this in vitro study, it was concluded that incorporation of disinfectants in die stone during mixing did not affect the hardness value at 24-hour interval. Dimensional accuracy was altered by iodophor and sodium hypochlorite, but remained unaffected by chlorhexidine and glutaraldehyde. How to cite this article Bakshi A, Dang R. Effect of Incorpo- ration of Disinfectants on Surface Hardness and Dimensional Accuracy of Die Stone An in vitro Study. Int J Prosthodont Restor Dent 2017;7(3):86-91.

scholarly journals Electron Microscopic Comparative Analysis of Smear Layer Removal by Ethylenediaminetetraacetic Acid and Chitosan Using Ultrasonic Activation: An In Vitro Study

2019 ◽  
Vol 4 (1) ◽  
pp. 37-41
Author(s):  
Arthanarieswaran A Sivakumar ◽  
Anjaneya S Prasad ◽  
Saravanapriyan Soundappan ◽  
Chakravarthy S Vineetha ◽  
Ravi Vaiyapuri ◽  
...  
Keyword(s):  
Comparative Analysis ◽  
Ethylenediaminetetraacetic Acid ◽  
In Vitro Study ◽  
Smear Layer ◽  
Vitro Study ◽  
Electron Microscopic ◽  
Ultrasonic Activation ◽  
Layer Removal

scholarly journals The influence of vinegars on exposure of dentinal tubules: a SEM evaluation

2004 ◽  
Vol 18 (1) ◽  
pp. 63-68 ◽  
Author(s):  
Daniela Leal Zandim ◽  
Fernanda Oliveira Bello Corrêa ◽  
José Eduardo Cezar Sampaio ◽  
Carlos Rossa Júnior
Keyword(s):  
Dietary Habits ◽  
In Vitro Study ◽  
Smear Layer ◽  
Control Group ◽  
Dentin Hypersensitivity ◽  
Human Teeth ◽  
Painful Condition ◽  
Dentinal Tubules ◽  
Significant Difference

Dentin hypersensitivity is a common painful condition observed in clinics. Dietary habits have been much associated with its development and persistence during and following periodontal treatment. The aim of this in vitro study was to evaluate the influence of vinegars on the removal of smear layer and exposure of dentinal tubules. Extracted human teeth were submitted to manual scaling with Gracey curettes in order to remove the cementum as well as to form a smear layer. Dentin samples with 3 mm² were obtained and distributed into six experimental groups: one control and five types of vinegars (alcohol, apple, rice, white wine and balsamic). Each group included two methods of vinegar application: topical and friction. After routine preparation for SEM analysis, photomicrographs were assessed by a calibrated and blind examiner using an appropriate index system. Kruskal-Wallis test indicated a significant influence of vinegars on smear layer removal. There was a statistically significant difference between groups treated with apple, white and rice vinegars and the control group (p < 0.05). Nevertheless, Mann-Whitney test indicated that removal of smear layer did not vary with the method of application (topical versus friction) for any of the tested substances. We can conclude that the contact of vinegar may remove smear layer and expose dentinal tubules, regardless of the type of application. However, balsamic vinegar was associated with less removal of smear layer after both methods of application.

scholarly journals Apical extrusion of sodium hypochlorite irrigation during root canal treatment using monoject or hypodermic irrigation needle

2016 ◽  
Vol 15 (4) ◽  
pp. 575-578
Author(s):  
Ahmad Fahmi Ariffin ◽  
Mohd Hafizal Harudin ◽  
S Kanagasingam ◽  
MM Rahman ◽  
WA Wan Noorina
Keyword(s):  
Sodium Hypochlorite ◽  
Root Canal ◽  
Medical Science ◽  
In Vitro Study ◽  
Root Canal Treatment ◽  
Hypodermic Needle ◽  
Anterior Teeth ◽  
Diffusion Technique ◽  
Apical Extrusion

Objective: Sodium hypochlorite has been routinely delivered into the pulp canal via irrigation needle. The study aimed at to determine the advantage and disadvantage of apical extrusion of sodium hypochloride using monoject or hypodermic irrigation needle in root canal treatment.Materials and Methods: Eighty single rooted teeth with closed apices were used in this in vitro study to determine apical extrusion of sodium hypochlorite using the gel diffusion technique. Extracted human anterior teeth were used as study samples. Monoject (size 27G) and hypodermic (27G× ½, 0.40×13mm, Terumo Needle) irrigation needle were used. The highest concentration of 5.25% hypochlorite solution was used for irrigation. To standardize the time diffusion of the dye, the gel was photographed at exactly 20 minutes after the initial irrigation with sodium hypochlorite.Results: A total of 36 out of 40 (90%) teeth in the hypodermic needle group showed positive apical extrusion compared to 14 of 40 teeth (35%) in the monoject group regardless of apical size. The discoloration of agar was clearly obtained after the sample tooth had been irrigated with sodium hypochlorite indicating the sign of apical extrusion. It is therefore; recommend that monoject irrigation needle should be used by students regardless of costs during root canal treatment in the polyclinic or dental clinic due to its safety in order to prevent sodium hypochlorite accident.Conclusion: Monoject needle showed significantly less sodium hypochlorite extrusion compared to hypodermic needleBangladesh Journal of Medical Science Vol.15(4) 2016 p.575-578

Sign in / Sign up

Export Citation Format

Share Document