Comparative Study to Evaluate the Surface Detail Reproduction of Dental Stone after Immersion in Various Different Disinfectant Solutions, Under Stereomicroscope 10 X Magnification –An In-Vitro Study

2017 ◽  
Vol 9 (3) ◽  
Author(s):  
Rathika Rai ◽  
M. A. Easwaran ◽  
K. T. Dhivya
Keyword(s):  
Sodium Hypochlorite ◽  
In Vitro Study ◽  
Control Group ◽  
Immersion Method ◽  
Significant Difference ◽  
Surface Irregularities ◽  
Disinfectant Solution ◽  
Surface Detail ◽  
Group B

Aim: To evaluate the surface detail reproduction of dental stone this is immersed in different disinfectant solution and studied under stereomicroscope. Methodology: Total number of 30 specimens of dental stone (Type III) were made with measurements of 1.5cm diameter and 1cm height .This samples are divided in to 3 groups group A,B,C. were A is immersed in Distilled water which was taken as control group ;B is immersed in 2% Glutaraldehyde and C is immersed in 5%sodium hypochlorite. Each specimen were immersed in the disinfectant solution for 15 minutes and dried under room temperature for 24 hrs. After 24 hrs each specimens are studied under stereomicroscope for surface details. Result: The results showed no significant difference in the surface irregularities and porosities for a group 1 and group 2 except group 3 which showed significant increase in the porosities, surface irregularities and erosions after disinfection with 5% NaHOCl by immersion method. Conclusion: The surface detail reproduction capacity of die stone was adversely affected when 5% Sodium hypochlorite was used as disinfectant solution when compare d to control group and 2% Glutaraldehyde

Sealing Ability of ProRoot MTA and MTA-Angelus Simulating a One-Step Apical Barrier Technique- An in vitro Study

2009 ◽  
Vol 33 (4) ◽  
pp. 305-310 ◽  
Author(s):  
Nikita Lolayekar ◽  
Sham Bhat ◽  
Sundeep Hegde
Keyword(s):  
Calcium Hydroxide ◽  
In Vitro Study ◽  
Control Group ◽  
Blue Dye ◽  
Root Segments ◽  
Sealing Ability ◽  
Significant Difference ◽  
Group A ◽  
Group B

Endodontic treatment of the pulpless tooth with an immature root apex poses a special challenge for the clinician due to lack of an apical stop against which to compact an interim dressing of calcium hydroxide, or the final obturation material. A one-visit apexification protocol with MTA (Mineral Trioxide Aggregate) has be seen as an alternative to the traditional treatment practices with calcium hydroxide [Ca(OH)2]. The aim of this study was to investigate the use of MTA as a 5mm apical barrier by comparing sealing ability of ProRoot MTA to that of MTA-Angelus. 50 freshly extracted Maxillary and Mandibular single canal sound human incisor teeth were used in this study, which were decoronated to standardized 10mm root lengths. The root segments were prepared to simulate the clinical situation of an open apex with Gates Glidden burs # 5-1 and randomly assigned into 2 experimental groups of 20 samples each (Group A: ProRoot MTA, Group B: MTA-Angelus) and 10 root segments were used as control (Group C) where no apical barrier was used. After obturation the coronal portion of all samples were sealed with Glass Ionomer and stored at 37°C and 100% humidity for 4 weeks. The root segments were then double coated with nail varnish except for the apical 1mm and apical ends of all root segments were suspended vertically in methylene blue dye for 48 hours at room temperature. After removal from the dye, the samples were sectioned longitudinally and linear dye penetration was measured using a Grid and stereomicroscope. The measurements were tabulated and statistically analyzed. The mean percentage of dye leakage for Group A was 55.5% and for Group B was 53.25%. The results showed no statistically significant difference in the sealing ability of ProRoot MTA and MTA-Angelus when used as apical barriers.

An In Vitro Evaluation of the Effect of Dimethyl Sulfoxide Pre-Treatment on the Bond Strength of Fiber Post

2021 ◽  
Vol 13 (4) ◽  
pp. 258-262
Author(s):  
Reshma Rajasekhar ◽  
Sooraj S ◽  
Midhula Sathyan ◽  
Varsha Maria Sebastian
Keyword(s):  
Bond Strength ◽  
Dimethyl Sulfoxide ◽  
In Vitro Study ◽  
Treated Group ◽  
Control Group ◽  
Significant Difference ◽  
Pre Treatment ◽  
Group B ◽  
Post Space

Background: The purpose of this in vitro study was to evaluate the efficacy of dimethyl sulfoxide pre-treatment of dentin on the bond strength of fiber post. Methods: Twenty single rooted mandibular premolar teeth were decoronated and shaping and cleaning was done till Protaper gold F3. After obturation, the teeth were sealed using type II GIC and stored in the distilled water for one week. Post space preparation was done using peeso reamer to a depth of 12 mm with 5 mm of remaining gutta percha in the apical portion of all samples and post space was etched with 37% phosphoric acid. The specimens were divided into two groups with 10 teeth each in group A (DMSO group) and group B (Control). In the group A, the post space was treated with 1 mL of 50% v/v dimethyl sulfoxide (DMSO) and in group B (control), the post space was treated with 1 mL of distilled water for 60 seconds with the help of paper points. Etch and rinse resin cement (Variolink II, IvoclarVivadent) was used following the manufacturer’s instructions. Fibre posts were coated with cement and placed into the post space and light cured. Push out strength of samples was performed using universal testing machine. Results: Statistical analysis of the data showed no significant difference in push out bond strength between the control group and pre-treated group. Conclusion: This in vitro study showed that there was no significant difference in the immediate bond strength scores of 50% v/v dimethyl sulfoxide pre-treated group when compared to control group. Keywords: Dentin, Dimethyl sulfoxide, Dowel, Resin cements

scholarly journals In vitro study of human osteoblast proliferation and morphology on orthodontic mini-implants

2015 ◽  
Vol 85 (6) ◽  
pp. 920-926 ◽  
Author(s):  
Ricardo Carvalho Bueno ◽  
Roberta Tarkany Basting
Keyword(s):  
Chemical Composition ◽  
In Vitro Study ◽  
Chemical Components ◽  
Control Group ◽  
Osteoblast Proliferation ◽  
Mini Implants ◽  
Significant Difference ◽  
Implant Anchorage ◽  
Positive Effect

ABSTRACT Objective:  To evaluate the proliferation and morphology of human osteoblasts cultured on two brands of mini-implants after 24, 48, and 72 hours, in addition to the chemical composition found on their surface. Materials and Methods:  Two brands of mini-implant (Morelli and Neodent) were evaluated; polystyrene was used as a control group (n  =  3). Osteoblasts were cultured on the surface of sterilized mini-implants in a CO2 incubator at different time periods (24, 48, and 72 hours). Osteoblast proliferation was quantified by scanning electron microscopy using up to 5000× magnification, and cell morphology was analyzed by a single observer. For the chemical analysis, spectroscopy X-ray fluorescence was used to identify and quantify chemical components on the surface of the mini-implants. Results:  Two-way ANOVA showed no significant interaction between the factors studied (P  =  0.686). A Tukey test revealed no significant difference in osteoblast proliferation between the mini-implants at all studied periods; however, a difference in cell proliferation was detected between the Neodent and the control group (P  =  .025). For all groups, time had a direct and positive effect on osteoblast proliferation (P < .001). The significant elements present in both brands of mini-implants were titanium, aluminum, vanadium, and iron. Conclusions:  Osteoblast proliferation was present on the mini-implants studied, which increased over time; however, no significant difference between brands was observed. No difference was seen between the mini-implants evaluated in terms of chemical composition. Cell adhesion after 72 hours suggests that areas of bone remodeling can be achieved, thus initiating the process of mini-implant anchorage.

scholarly journals The Effect of the Bioactive Glass and the Er:YAG Laser on the Remineralization of the Affected Dentin: A Comparative In Vitro Study

2020 ◽  
Vol 11 (2) ◽  
pp. 160-166
Author(s):  
Mohammad Javad Moghaddas ◽  
Horieh Moosavi ◽  
Sara Yaghoubirad ◽  
Nasim Chiniforush
Keyword(s):  
Bioactive Glass ◽  
In Vitro Study ◽  
Control Group ◽  
Dentinal Tubule ◽  
Glass Ionomer ◽  
Significant Difference ◽  
Post Hoc ◽  
Caries Lesions ◽  
Cavity Floor

Introduction: The purpose of this study was to compare the effect of the bioactive glass, the glass ionomer, and the Erbium YAG laser as liners on the remineralization of the affected dentin. Methods: The present study was conducted on 64 healthy extracted human molars divided into 4 groups, 1 control group and 3 experimental groups. After artificially inducing dentinal caries lesions, each of the experimental groups was applied to the cavity floor and then restored with a composite. The samples were stored after thermocycling in an incubator for two months. Finally, the hardness of the cavity floor was measured at 3 depths of 20, 50 and 100 μm by the Vickers microhardness tester. The dentin conditions underneath the liners were also evaluated with FESEM. Statistical analysis was performed by two-way ANOVA and the post-hoc Games-Howell test (P<0.05). Results: Among the groups, the lowest microhardness value was in the control group (P<0.05) except at a depth of 100 μm; therefore, there was no significant difference between the control group and the bioactive glass (P>0.05). The laser group had the highest microhardness value, which was significantly different from the control group (P<0.05). There was a significant difference between the laser and bioactive glass (P<0.05), except at a depth of 20 μm. The laser and glass ionomer had only a significant difference at a depth of 100 μm (P<0.05). The microhardness value induced by glass ionomer was higher than bioactive glass, which in no depth was significant (P>0.05). Partial dentinal tubule occlusion was observed with FESEM in each of the experimental groups as compared to the control group. Conclusion: The microhardness values were higher in all groups than in the control group. The laser might be more successful in remineralization than the other ones.

scholarly journals Milled versus moulded mock-ups based on the superimposition of 3D meshes from digital oral impressions: a comparative in vitro study in the aesthetic area

2019 ◽  
Vol 19 (1) ◽  
Author(s):  
Francesca Cattoni ◽  
Giulia Teté ◽  
Alessandro Mauro Calloni ◽  
Fabio Manazza ◽  
Giorgio Gastaldi ◽  
...  
Keyword(s):  
Statistical Analysis ◽  
Test Group ◽  
In Vitro Study ◽  
Mean Value ◽  
Digital Impression ◽  
Control Group ◽  
Minimal Invasiveness ◽  
Significant Difference ◽  
The Aesthetic

Abstract Background Aesthetic porcelain veneers proved to be a long-term reliable prosthetic solution, ensuring minimal invasiveness. The use of veneers requires an adhesive cementation technique, so maintaining as much enamel as possible is to ensure lasting success. A diagnostic mock-up is a key tool that allows a preview of the outcome of the aesthetic restoration: it is obtainable both in an analog and digital way. With the recent developments in impression technology and the ever so fast growing use of CAD-CAM technologies it is useful to understand the pros and cons of either one of these techniques (analog and digital) in order to identify the easier and more convenient workflow in aesthetic dentistry. Methods After taking pictures and impressions of the dental arcs of a patient in need of aesthetic rehabilitation, 52 resin models were produced and a digital drawing of the smile was outlined. Both an analog and a digital wax-up were obtained from two of the 52 models: the latter was obtained using digital impressions and a dedicated software. The analog wax-up was then used to produce 25 matrices that have later been used to mould 25 resin mock-ups using a traditional moulding protocol (Control Group - CG). The digital wax-up was used to mill 25 PMMA mock-ups. Each mock-up, both milled and moulded (total 50), was then laid on the other 50 resin models as a digital impression of it was taken. The STL. files of the milled mock-ups were compared with the 3D CAD wax-up made using a specific software. The STL. files of the analog printed mock-ups were compared with the traditional wax-up design. A statistical analysis was carried out to evaluate the difference between the groups. Results The statistical analysis showed a significant difference (P > 0.01) between the mean value of the distance between the points of the overlapping STL. meshes in GC (0.0468 mm) and in TG (Test Group - TG) (0,0109 mm). Conclusions The study showed a difference in accuracy between traditional moulded and milled mock-ups compared to their original wax-up. The data analysis reports that the digital method allows for greater accuracy. Within the limitations of this study, a fully digital workflow is to considered more reliable when it come to creating an esthetic mockup: the digital procedure has been shown to be more accurate than the one made manually which is much more operator dependent and it brings an increase to the chance of error, and that could ultimately affect the final result.

Inhibitory Effect of Resveratrol on the Pharmacokinetics of Ticagrelor in Vivo and Vitro

2021 ◽  
Author(s):  
Peng Wang ◽  
Xiao-Xia Hu ◽  
Ying-hui Li ◽  
Nan-Yong Gao ◽  
Guo-quan Chen ◽  
...  
Keyword(s):  
Rat Liver ◽  
Liver Microsomes ◽  
In Vitro Study ◽  
Inhibitory Effect ◽  
Control Group ◽  
Rat Liver Microsomes ◽  
Sprague Dawley ◽  
Group B

This study was to evaluate the effect of resveratrol on the pharmacokinetics of ticagrelor in rats and the metabolism of ticagrelor in human CYP3A4 and liver microsomes. Eighteen Sprague-Dawley rats were randomly divided into three groups: group A (control group), group B (50mg/kg resveratrol), and group C (150mg/kg resveratrol ). After 30 minutes administration of resveratrol, a single dose of ticagrelor (18mg/kg) was administered orally. The vitro experiment was performed to examine the influence of resveratrol on ticagrelor metabolism in CYP3A4*1, human, and rat liver microsomes. Serial biological samples were assayed by validated UHPLC-MS/MS methods. In vivo study, the AUC and Cmax of ticagrelor in group B and C appeared to be significantly higher than the control group, while Vz/F and CLz/F of ticagrelor in group B and C were significantly decreased. In vitro study, resveratrol exhibited an inhibitory effect on CYP3A4*1, human and rat liver microsomes. The IC50 values of resveratrol were 56.75μM，69.07μM and 14.22μM, respectively. Our results indicated that resveratrol had a inhibitory effect on the metabolism of ticagrelor in vitro and vivo. It should be paid more attention to the clinical combination of resveratrol with ticagrelor and ticagrelor plasma concentration should be monitored to avoid the occurrence of adverse reaction.

scholarly journals To Evaluate the Marginal Fit of Metal Copings Fabricated by Conventional Casting Procedure and Direct Metal Laser Sintering Technology – an In Vitro Study

2017 ◽  
Vol 05 (01) ◽  
pp. 039-046 ◽  
Author(s):  
Monica Sharma ◽  
Ajay Bansal ◽  
Sunny Panthi ◽  
Shefali Malik ◽  
Atulya Sharma
Keyword(s):  
Glass Ionomer Cement ◽  
In Vitro Study ◽  
Laser Sintering ◽  
Vitro Study ◽  
Direct Metal Laser Sintering ◽  
Marginal Fit ◽  
Significant Difference ◽  
Group A ◽  
Group B

Abstract Purpose: The purpose of this in vitro study was to evaluate marginal fit of cobalt- chromium (Co-Cr) copings fabricated by direct metal laser sintering system (DMLS) and conventional lost-wax technique (LW). Materials and method: Forty tooth preparations were carried out over extracted mandibular molars. They were divided into two groups A and B of 20 each. For group A Co-Cr copings were fabricated by direct metal laser sintering (DMLS) and for group B by lost wax technique (LW). Glass –ionomer cement (GIC) was used to tack the copings over their preparations. Marginal fit was then evaluated directly under the stereomicroscope. Results: The mean marginal gap of group A was 27.9 ± 2.4 μm and group B was 40.4 ±6 μm. Statistical analysis using t - test showed highly significant difference (P>.05) between the marginal mean of the DMLS (group A) compared to LW (group B). Conclusion: The DMLS copings demonstrated superior marginal fit compared to that of conventional Co-Cr casted copings.

scholarly journals Evaluation of the Effect of Water on Three Different Light Cured Composite Restorative Materials Stored in Water: An In Vitro Study

2012 ◽  
Vol 2012 ◽  
pp. 1-5 ◽  
Author(s):  
Basawaraj Biradar ◽  
Sudharani Biradar ◽  
Arvind MS
Keyword(s):  
Water Absorption ◽  
Physical And Mechanical Properties ◽  
In Vitro Study ◽  
Vitro Study ◽  
Weight Changes ◽  
Significant Difference ◽  
Restorative Materials ◽  
Group A ◽  
Group B

Objectives. The objective of this in vitro study was to investigate whether weight gain or loss in the three different composites occurs due to water absorption when they are stored in water.Methods. The composite restorative materials selected for this study included a microfine hybrid (Synergy) and two nanofilled composite restorative materials (Ceram X and Filtek Supreme Ultra). Twenty specimens of each material were fabricated of each composite material. Group A: Filtek Supreme Ultra, Group B: Synergy, Group C: Ceram X. Then all the specimens were stored in 10 ml Distilled water containing test tubes and placed in incubator at 37°C for six weeks. The weight changes of these specimens were measured daily for the first week and later once a week for next five weeks by using an electrical analytical balance.Results.The data was analyzed by one-way analysis of variance and Student'sttest. All groups showed maximum amount of water absorption in the first week than gradual decrease in the water absorption from the second to the sixth week, as compared to the first week and there is no statistically significant difference between the groups tested.Conclusion. All the composite restorative material absorbs some amount of water. The water absorption of the composite may decrease the physical and mechanical properties of the composites; hence it is necessary to consider the type of the material before starting the treatment.

EVALUATION OF THE EFFECT OF FERROUS SULFATE ON ENAMEL DEMINERALIZATION OF HUMAN DECIDUOUS TEETH: AN IN VITRO STUDY

2017 ◽  
Vol 8 (3) ◽  
pp. 83-89
Author(s):  
Johnny Holanda De Gauw ◽  
Lara Maria Melo Costa ◽  
Rodrigo Neves Silva ◽  
Natanael Barbosa Santos ◽  
Maria Dânia Holanda Tenorio
Keyword(s):  
Ferrous Sulfate ◽  
Polarized Light ◽  
In Vitro Study ◽  
Deciduous Teeth ◽  
Control Group ◽  
Group A ◽  
Ph Cycling ◽  
Group B ◽  
Group D

Objective: This study aimed to evaluate the effect of ferrous sulfate (FS) on demineralized and non-demineralized human deciduous teeth. Additionally, it was evaluated the penetration extent of FS and its remineralizing effect on the enamel of deciduous teeth using Polarized Light Microscopy (PLM). Method: The sample comprised 44 human deciduous teeth. The 44 crowns were divided randomly into four groups: group A (FS after demineralization), group B (FS without demineralization), group C (only demineralization), and group D (control group). FS at 0.45 mol/L-1 was used daily (15 days) and demineralization was done by pH cycling (7 days). Then, three longitudinal slices of the crowns were photographed using PLM. The degree of penetration of the lesion or stain was measured in micrometers, as well as the distance between the external enamel surface and the core of lesion. Results: Group A showed a dark stain on the outer surface of enamel larger than the group B. It is suggested, a remineralizing effect when comparing groups, A and C. The mean depth and standard deviation for groups A, B, and C were 4.27µm (±1.49), 3.72 µm (±1.68) and 5.00 µm (±1.84), respectively. No dark stains were observed in group D. Conclusion: FS stained the demineralized and non-demineralized human deciduous teeth. However, dark stains in the non-demineralized teeth were smaller or absent, than in the demineralized teeth. Therefore, FS may have a protective effect against demineralization.

Influence of prophylaxis on the microleakage of sealants: in vitro study

2002 ◽  
Vol 26 (4) ◽  
pp. 371-375 ◽  
Author(s):  
Ana Beatriz Alonso Chevitarese ◽  
Orlando Chevitarese ◽  
Ivete Pomarico Ribeiro de Souza ◽  
Roberto Braga de Carvalho Vianna
Keyword(s):  
Electron Microscope ◽  
Scanning Electron Microscope ◽  
In Vitro Study ◽  
Optical Microscope ◽  
Vitro Study ◽  
Significant Difference ◽  
Group A ◽  
Group B ◽  
Scanning Electron

The aim of this study was to evaluate the influence of prophylaxis on the sealants microleakage in 30 premolars divided into: Group A, Group B and Group C. The teeth were analyzed using the optical microscope (OM) and at scanning electron microscope (SEM). There was a statitical significant difference among the groups regarding the presence of microleakage, but not with the presence of tags.

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