scholarly journals Cases of amyloidosis with diabetic encephalopathy

2018 ◽  
Vol 20 (1) ◽  
pp. 58-62
Author(s):  
V I Golovkin ◽  
D A Gulak ◽  
T A Garan ◽  
I P Magonov
Keyword(s):  
Protein Metabolism ◽  
Congo Red ◽  
Serum Amyloid A ◽  
Interleukin 1 ◽  
Clinical Manifestations ◽  
Serum Amyloid ◽  
High Rate ◽  
Diabetic Encephalopathy ◽  
Biopsy Material ◽  
Amyloid A

Basic clinical manifestations of diabetic encephalopathy in pre-stroke and stroke stages in the elderly are considered. Psychometrical tests (Shulte tables of and Mini Mental Score Examination scale) were used to reveal cognitive impairments, which are markers of diabetic encephalopathy progression. The case of intravital visualization of diabetic cerebral angiopathy using magnetic resonance imaging in susceptibility weighted imaging mode was described in detail. And the case of amyloidosis confirmed by kidney biopsy material coloring with Congo Red. The results of immunological examination are given, proving a high rate of Interleukin-1 production - initiator of serum amyloid A synthesis in liver (serum amyloid A). Histories of lethal cases and pathohystological analysis results of ultrathin sections, obtained by the means of cerebrum autopsy with Congo Red coloring, were investigated. Autopsy materials with positive qualitative reaction on amyloid were taken for further analyzing in polarizing light and amyloid typing. AA-amyloid was discovered in all cases. Morphologic characteristic of diabetic encephalopathy was revealed using coloring by hematoxylin, eosin and Van Gieson’s stain: angioedema, microhemorrhagia, leukoaraiosis, gliomatosis and atrophy of neurons. Case of genetic polyorganic AA- amyloidosis, not diagnosed intravital, was described in detail. It was established that impaired protein metabolism with its final conformation in toxic amyloid components of tissues is an early and fairly frequent manifestation of diabetic encephalopathy metabolic disorders. The substantiated opinion, implying the necessity of deep protein metabolism investigation in cases of diabetes complicated with encephalopathy and amyloidosis, is given. The term «diabetic amyloid encephalopathy» is offered to include in diabetic encephalopathy classification.

scholarly journals Regulation of rabbit acute phase protein biosynthesis by monokines

1988 ◽  
Vol 253 (3) ◽  
pp. 851-857 ◽  
Author(s):  
A Mackiewicz ◽  
M K Ganapathi ◽  
D Schultz ◽  
D Samols ◽  
J Reese ◽  
...  
Keyword(s):  
Acute Phase ◽  
Serum Amyloid A ◽  
Interleukin 1 ◽  
Serum Amyloid ◽  
C Reactive Protein ◽  
Reactive Protein ◽  
Amyloid A ◽  
Primary Hepatocyte Cultures ◽  
Dose Dependent ◽  
Necrosis Factor

We defined the acute phase behaviour of a number of rabbit plasma proteins in studies (in vivo) and studied the effects of monokine preparations on their synthesis by rabbit primary hepatocyte cultures. Following turpentine injection, increased serum levels of C-reactive protein, serum amyloid A protein, haptoglobin, ceruloplasmin, and decreased concentrations of albumin were observed. In contrast to what is observed in man, concentrations of alpha 2-macroglobulin and transferrin were increased. Co-culture of primary hepatocyte cultures with lipopolysaccharide-activated human peripheral blood monocytes or incubation with conditioned medium prepared from lipopolysaccharide-activated human or rabbit monocytes resulted in dose-dependent induction of serum amyloid A, haptoglobin, ceruloplasmin and transferrin and depression of albumin synthesis, while C-reactive protein synthesis and mRNA levels remained unchanged. A variety of interleukin-1 preparations induced dose-dependent increases in the synthesis and secretion of serum amyloid A, haptoglobin, ceruloplasmin and transferrin and decreased albumin synthesis. Human recombinant tumour necrosis factor (cachectin) induced a dose-dependent increase in synthesis of haptoglobin and ceruloplasmin. In general, human interleukin-1 was more potent than mouse interleukin-1 and tumour necrosis factor. None of the monokines we studied had an effect on C-reactive protein synthesis or mRNA levels. These data confirm that C-reactive protein, serum amyloid A, haptoglobin and ceruloplasmin display acute phase behaviour in the rabbit, and demonstrate that, in contrast to their behaviour in man, alpha 2M and transferrin are positive acute phase proteins in this species. While both interleukin-1 and tumour necrosis factor regulate biosynthesis of a number of these acute phase proteins in rabbit primary hepatocyte cultures, neither of these monokines induced C-reactive protein synthesis. Comparison of these findings with those in human hepatoma cell lines, in which interleukin-1 does not induce serum amyloid A synthesis, suggests that the effect of interleukin-1 on serum amyloid A synthesis may be indirect.

THE ROLE OF INTERLEUKIN 1 IN ACUTE PHASE SERUM AMYLOID A (SAA) AND SERUM AMYLOID P (SAP) BIOSYNTHESIS

1982 ◽  
Vol 389 (1 C-Reactive Pr) ◽  
pp. 137-150 ◽  
Author(s):  
Jean D. Sipe ◽  
Stefanie N. Vogel ◽  
Marcela B. Sztein ◽  
Martha Skinner ◽  
Alan S. Cohen
Keyword(s):  
Acute Phase ◽  
Serum Amyloid A ◽  
Interleukin 1 ◽  
Serum Amyloid ◽  
Amyloid A ◽  
Serum Amyloid P ◽  
Amyloid P ◽  
Acute Phase Serum

scholarly journals Serum amyloid A induces interleukin-6 in dermal fibroblasts via Toll-like receptor 2, interleukin-1 receptor-associated kinase 4 and nuclear factor-κB

Immunology ◽  
2014 ◽  
Vol 143 (3) ◽  
pp. 331-340 ◽  
Author(s):  
Steven O'Reilly ◽  
Rachel Cant ◽  
Marzena Ciechomska ◽  
James Finnigan ◽  
Fiona Oakley ◽  
...  
Keyword(s):  
Interleukin 6 ◽  
Serum Amyloid A ◽  
Interleukin 1 ◽  
Nuclear Factor Κb ◽  
Dermal Fibroblasts ◽  
Serum Amyloid ◽  
Nuclear Factor ◽  
Toll Like Receptor ◽  
Amyloid A ◽  
Toll Like Receptor 2

scholarly journals Regulation of mouse serum amyloid A gene expression in transfected hepatoma cells.

1990 ◽  
Vol 10 (7) ◽  
pp. 3619-3625 ◽  
Author(s):  
J H Huang ◽  
H Y Rienhoff ◽  
W S Liao
Keyword(s):  
Serum Amyloid A ◽  
Cultured Cells ◽  
Regulatory Element ◽  
Interleukin 1 ◽  
Fold Increase ◽  
Conditioned Media ◽  
Serum Amyloid ◽  
Mouse Serum ◽  
Amyloid A ◽  
Mouse Serum Amyloid

Expression of mouse serum amyloid A (SAA1, -2, and -3) mRNAs can be induced up to 1,000-fold in the liver in response to acute inflammation. This large increase is primarily the result of a 200-fold increase in the rates of SAA gene transcription. To analyze the cis-acting regulatory element(s) responsible for regulating transcription, we fused 306 base pairs of the mouse SAA3 promoter to a reporter gene, the chloramphenicol acetyltransferase (CAT) gene, and transfected this chimeric DNA into cultured cells. In transient expression assays, this 5' sequence was sufficient to confer cell-specific expression: CAT activity was readily detectable when the construct was transfected into liver-derived cells but was not detectable in nonliver cells. Furthermore, when liver cells transfected with this construct were treated with conditioned media prepared from activated mixed lymphocyte cultures or with recombinant interleukin-1, a 10- to 15-fold increase in CAT activity was detected. Deletion analyses showed two regions of interest: a proximal region that enhanced CAT expression in a cell-specific manner and a distal region that conferred responsiveness to both conditioned media and recombinant interleukin-1. This distal responsive element had properties of an inducible transcriptional enhancer, and deletion of the proximal cell-specific region rendered the distal element responsive to stimulation by conditioned media in nonliver cells.

scholarly journals Correlation of serum amyloid A levels, clinical manifestations, treatment, and disease activity in patients with acute anterior uveitis

Eye ◽  
2019 ◽  
Vol 34 (9) ◽  
pp. 1672-1678
Author(s):  
Ma-Li Dai ◽  
Shipei Fan ◽  
Zhuoran Li ◽  
Xuewen Yu ◽  
Dan Lin ◽  
...  
Keyword(s):  
Disease Activity ◽  
Anterior Uveitis ◽  
Serum Amyloid A ◽  
Clinical Manifestations ◽  
Serum Amyloid ◽  
Acute Anterior Uveitis ◽  
Amyloid A
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