Registro de Herpesvírus Bovino (BoHV-1 e BoHV-5) em Rebanho Leiteiro de Propriedades Agro Familiares da Cidade de Alegrete – RS

Author(s):  
Phelipe Magalhães Duarte ◽  
Vivian Tallita Pinheiro Santana

As doenças reprodutivas se constituem um dos principais motivos de prejuízo na bovinocultura, representando uma parcela significativa da diminuição dos índices de prenhes e animais nascidos. Entre estas doenças, a infecção por Herpesvírus Bovino (BoHV) tem grande destaque, principalmente, por sua grande disseminação no rebanho brasileiro. O BoHV-1 é o agente etiológico relacionado com a rinotraqueíte infecciosa bovina (IBR), aborto e reabsorção embrionária, além de infecções em nível reprodutivos e neonatais. Já o BoHV-5 é o agente responsável pela encefalite herpética bovina. Entre os diversos métodos de diagnóstico, a soroneutralização é utilizada rotineiramente para evidenciar a presença de anticorpos neutralizantes para o vírus. O presente trabalho teve por finalidade diagnosticar a prevalência sorológica por Soroneutralização (SN), referente ao herpesvírus bovino tipo 1 e tipo 5 no rebanho leiteiro, não vacinado, de propriedades agro familiares de assentados da reforma agrária localizados na cidade de Alegrete - RS. Nas avaliações realizadas para o presente estudo foram registradas proporções de 40,90% e 39,39% de animais soropositivos para os agentes BoHV-1 e BoHV-5, respectivamente. Os resultados obtidos sugerem que é necessário maior empenho no controle da enfermidade, uma vez que possui fácil disseminação. Além disso, os dados evidenciam o cenário da bovinocultura brasileira, em que há presença significativa de agentes potenciais, que resultam em diminuição das taxas reprodutivas, somada a uma cultura de não prevenção. Palavras-chave: Virologia. Herpesvírus Bovino 1.  Herpesvírus Bovino 5. AbstractThe reproductive diseases are one of the main reasons for the losses in bovine culture, being a significative part of the reduction in the numbers of pregnant and newborn animals. Among those diseases, the Bovine Herpesvirus infection (BoHV) is on the spotlight, mainly because of the great dissemination in  the Brazilian herd. BoHV-1 is the etiological agent related to the infectious bovine rhinotracheitis (IBR), abortion and embryony reabsorption, besides infections at reproductive and neonatal levels. Whereas, BoHV-5 is the agent responsible for the bovine herpetic encephalitis. Among the various methods of diagnosis, serum neutralization is routinely used to evidence the presence of neutralizing antibodies to the virus. The present work had the goal to diagnose the serological prevalence by Serum Neutralization (SN), referring to bovine herpesvirus type 1 and type 5 in the non-vaccinated dairy herd, from agrarian reform settlers of  the agrarian reform located in the city of Alegrete – RS. In the evaluations carried out for the present study, ratios of 40.90% and 39.39% of seropositive animals were recorded for BoHV-1 and BoHV-5 agents, respectively. The results obtained suggest that a greater effort is required in the disease control disease, since it has an easy dissemination. In addition, the data show that the scenario of Brazilian cattle breeding, in which there is a significant presence of potential agents that result in a decrease in reproductive rates, together with a culture of non-preveiton.Keyword: virology. Herpesvirus 1; Bovine; Herpesvirus 5 Bovine.

2011 ◽  
Vol 152 (3-4) ◽  
pp. 270-279 ◽  
Author(s):  
S.I. Chowdhury ◽  
M.C.S. Brum ◽  
C. Coats ◽  
A. Doster ◽  
Huiyong Wei ◽  
...  

2017 ◽  
Vol 38 (6) ◽  
pp. 3915
Author(s):  
Greice Japolla ◽  
Ana Flávia Batista Penido ◽  
Greyciele Rodrigues Almeida ◽  
Luiz Artur Mendes Bataus ◽  
Jair Pereira Cunha Junior ◽  
...  

The specificity of monoclonal antibodies (mAbs) to desired targets makes these molecules suitable for therapeutic and diagnostic uses against a wide range of pathogens. Phage display antibody libraries offer one method by which mAbs can be selected for, without the use of conventional hybridoma technology. In this work, phage display technology was used to construct, select and characterize a combinatorial single chain fragment variable (scFv) antibody library against bovine herpesvirus type 1 (BoHV-1) from the immune repertoire of chickens immunized with the virus. In silico analysis of the hypervariable domains of the antibody heavy chains revealed a high frequency of scFv fragments with low variability, suggesting that selection had probably been carried out and favored by a few im-munogenic viral antigens. The reactivity of the scFv fragments selected against BoHV-1 was demon-strated by Phage-ELISA. A significant increase in antibody reactivity to the target was observed after six rounds of library selection, showing its potential use as a molecule for BoHV-1 diagnosis. The strategy described here opens up a field for the use of phage display as a tool for selection of mono-clonal antibodies that could be used for theranostic applications against infectious and parasitic dis-eases of veterinary interest.


2022 ◽  
Vol 10 (1) ◽  
pp. 188
Author(s):  
Filomena Fiorito ◽  
Claudia Cerracchio ◽  
Maria Michela Salvatore ◽  
Francesco Serra ◽  
Alessia Pucciarelli ◽  
...  

Bovine herpesvirus type-1 (BoHV-1) is a widespread pathogen that provokes infectious rhinotracheitis and polymicrobial infections in cattle, resulting in serious economic losses to the farm animal industry and trade restrictions. To date, non-toxic active drugs against BoHV-1 are not available. The exploitation of bioactive properties of microbial products is of great pharmaceutical interest. In fact, fungi are a promising source of novel drugs with a broad spectrum of activities and functions, including antiviral properties. Hence, the potential antiviral properties of 3-O-methylfunicone (OMF), a secondary metabolite produced by Talaromyces pinophilus, were evaluated on BoHV-1. In this study, during BoHV-1 infection in bovine cells (MDBK), the non-toxic concentration of 5 µM OMF considerably reduced signs of cell death and increased cell proliferation. Furthermore, OMF significantly decreased the virus titer as well as the cytopathic effect and strongly inhibited the expression of bICP0, the major regulatory protein in the BoHV-1 lytic cycle. These findings were accompanied by a considerable up-regulation in the expression of the aryl hydrocarbon receptor (AhR), a multifunctional transcription factor also linked to the host’s response to a herpesvirus infection. Overall, our results suggest that by involving AhR, OMF shows potential against a BoHV-1 infection.


2016 ◽  
Vol 109 ◽  
pp. 161-165 ◽  
Author(s):  
Yao-Chi Chung ◽  
Hsiu-Yen Shen ◽  
Li-Ting Cheng ◽  
Shyh-Shyan Liu ◽  
Chun-Yen Chu

2018 ◽  
Vol 163 (6) ◽  
pp. 1549-1557 ◽  
Author(s):  
Greice Japolla ◽  
Jair Pereira Cunha-Junior ◽  
Ana Claudia Arantes Marquez Pajuaba ◽  
Ernesto Akio Taketomi ◽  
Samira Bührer-Sékula ◽  
...  

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